FlyBase Gene Report: Dmel\CSN5

FB2024_03 , released June 25, 2024

FB2024_03 , released June 25, 2024

Gene: Dmel\CSN5

General Information

Symbol

Dmel\CSN5

Species

D. melanogaster

Name

COP9 signalosome subunit 5

Annotation Symbol

CG14884

Feature Type

protein_coding_gene

FlyBase ID

FBgn0027053

Gene Model Status

Stock Availability

11 publicly available

Gene Summary

COP9 signalosome subunit 5 (CSN5) encodes the subunit 5 of the COP9 signalosome (CSN). The CSN plays a central role in the regulation of E3-cullin RING ubiquitin ligases. The product of CSN5 is an isopeptidase that deNEDDylates the cullin subunit of E3-cullin RING ubiquitin ligases. [Date last reviewed: 2019-03-07] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
Gene Group
UniProtKB
Interactive Fly

Also Known As

DCH5, Jab1, l(3)L4032, COP9 complex homolog subunit 5, l(3)89Cf

Key Links

Genomic Location

Cytogenetic map

Sequence location

3R:16,470,099..16,471,406 [+]

Recombination map

3-58

RefSeq locus

NT_033777 REGION:16470099..16471406

Sequence

Get Decorated FASTA

Genomic Maps

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (23 terms)

Molecular Function (4 terms)

Terms Based on Experimental Evidence (2 terms)

CV Term

Evidence

References

enables metal-dependent deNEDDylase activity

inferred from direct assay

(Doronkin et al., 2003)

enables protein binding

inferred from physical interaction with FLYBASE:alien; FB:FBgn0013746

(Freilich et al., 1999)

inferred from physical interaction with UniProtKB:Q7KRW1

(Gemmill et al., 2002)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

enables deNEDDylase activity

inferred from biological aspect of ancestor with PANTHER:PTN000045369

(GO Reference Genome Project, 2011-)

enables metallopeptidase activity

inferred from electronic annotation with InterPro:IPR000555

(InterPro Project Members, 2004-)

inferred from biological aspect of ancestor with PANTHER:PTN000045368

(GO Reference Genome Project, 2011-)

Biological Process (16 terms)

Terms Based on Experimental Evidence (16 terms)

CV Term

Evidence

References

involved_in axonogenesis

inferred from mutant phenotype

(Suh et al., 2002)

involved_in compound eye photoreceptor cell differentiation

inferred from mutant phenotype

(Suh et al., 2002)

involved_in female germ-line stem cell population maintenance

inferred from mutant phenotype

(Pan et al., 2014)

involved_in germ cell development

inferred from mutant phenotype

(Qian et al., 2015)

involved_in germarium-derived cystoblast division

inferred from mutant phenotype

(Pan et al., 2014)

involved_in glial cell migration

inferred from mutant phenotype

(Suh et al., 2002)

involved_in male germ-line cyst encapsulation

inferred from mutant phenotype

(Qian et al., 2015)

involved_in negative regulation of hemocyte proliferation

inferred from mutant phenotype

(Harari-Steinberg et al., 2007)

involved_in negative regulation of lamellocyte differentiation

inferred from mutant phenotype

(Harari-Steinberg et al., 2007)

involved_in negative regulation of proteasomal ubiquitin-dependent protein catabolic process

inferred from mutant phenotype

(Ullah et al., 2007)

involved_in oocyte anterior/posterior axis specification

inferred from mutant phenotype

(Doronkin et al., 2002)

involved_in oocyte dorsal/ventral axis specification

inferred from mutant phenotype

(Doronkin et al., 2002)

involved_in positive regulation of cell cycle

inferred from mutant phenotype

(Ullah et al., 2007)

involved_in positive regulation of protein catabolic process

inferred from mutant phenotype

(Doronkin et al., 2003)

involved_in protein deneddylation

inferred from mutant phenotype

(Doronkin et al., 2003)

involved_in protein stabilization

inferred from mutant phenotype

(Ullah et al., 2007)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

involved_in protein deneddylation

inferred from biological aspect of ancestor with PANTHER:PTN000045369

(GO Reference Genome Project, 2011-)

Cellular Component (3 terms)

Terms Based on Experimental Evidence (3 terms)

CV Term

Evidence

References

part_of COP9 signalosome

inferred from direct assay

(Freilich et al., 1999)

located_in cytoplasm

inferred from direct assay

(Harari-Steinberg et al., 2007, Suh et al., 2002)

located_in nuclear periphery

inferred from direct assay

(Harari-Steinberg et al., 2007)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

part_of COP9 signalosome

inferred from biological aspect of ancestor with PANTHER:PTN000045369

(GO Reference Genome Project, 2011-)

is_active_in cytoplasm

inferred from biological aspect of ancestor with PANTHER:PTN000045369

(GO Reference Genome Project, 2011-)

Gene Group (FlyBase)

COP9 SIGNALOSOME

METALLO-TYPE DENEDDYLASES

COP9 SIGNALOSOME COMPLEX - TESTIS-SPECIFIC VARIANT

Protein Family (UniProt)

Belongs to the peptidase M67A family. CSN5 subfamily. (Q9XZ58)

Protein Signatures (InterPro)

Summaries

Gene Snapshot

COP9 signalosome subunit 5 (CSN5) encodes the subunit 5 of the COP9 signalosome (CSN). The CSN plays a central role in the regulation of E3-cullin RING ubiquitin ligases. The product of CSN5 is an isopeptidase that deNEDDylates the cullin subunit of E3-cullin RING ubiquitin ligases. [Date last reviewed: 2019-03-07]

Gene Group (FlyBase)

COP9 SIGNALOSOME -: The COP9 signalosome is an eight-subunit complex that is structurally and evolutionarily related to the 19S lid of the 26S proteasome. The COP9 signalosome plays a central role in the regulation of the E3-cullin RING ubiquitin ligases, deNEDDylating the cullin subunit via the isopeptidase activity of the CNS5 subunit. (Adapted from FBrf0225508 and FBrf0225509).
METALLO-TYPE DENEDDYLASES -: Neddylation is a post-translational protein modification that conjugates the ubiquitin-like protein NEDD8 to target proteins. Neddylation is countered by the action of deneddylases via a process termed deneddylation. By altering the substrate's conformation, stability, subcellular localization or binding affinity to DNA or proteins, neddylation regulates diverse cellular processes including the ubiquitin-proteasome system-mediated protein degradation, protein transcription and cell signaling. Only a minority of deneddylases, including the COP9 signalosome (CSN) and NEDP1 (aka DEN1/SENP8), are NEDD8-specific, whereas the majority are also known to regulate deubiquitination. Deneddylases may be metallopeptidases (this group; e.g. CSN) or cysteine-type peptidases (e.g. NEDP1). (Adapted from PMID:25628956.)
COP9 SIGNALOSOME COMPLEX - TESTIS-SPECIFIC VARIANT -: The COP9 signalosome is an eight-subunit complex that is structurally and evolutionarily related to the 19S lid of the 26S proteasome. The COP9 signalosome plays a central role in the regulation of the E3-cullin RING ubiquitin ligases, deNEDDylating the cullin subunit via the isopeptidase activity of the CNS5 subunit. (Adapted from FBrf0225508 and FBrf0225509).

Protein Function (UniProtKB)

Probable protease subunit of the COP9 signalosome complex (CSN), a complex involved in various cellular and developmental processes. The CSN complex is an essential regulator of the ubiquitin (Ubl) conjugation pathway by mediating the deneddylation of the cullin subunits of the SCF-type E3 ligase complexes, leading to decrease the Ubl ligase activity of SCF. In the complex, it probably acts as the catalytic center that mediates the cleavage of Nedd8 from cullins. It however has no metalloprotease activity by itself and requires the other subunits of the CSN complex. The CSN complex plays an essential role in oogenesis and embryogenesis and is required for proper photoreceptor R cell differentiation and promote lamina glial cell migration or axon targeting. It also promotes Ubl-dependent degradation of cyclin E (CycE) during early oogenesis. Also involved in regulation of axis formation by checkpoint-dependent, translational control of Gurken.

(UniProt, Q9XZ58)

Summary (Interactive Fly)

Component of COP9 signalsome - regulates ubiquitin-dependent protein degradation - Jab1/MPN domain metalloenzyme motif - COP9-Hedgehog axis regulates the function of the germline stem cell progeny differentiation niche

Interactive Fly

Gene Model and Products

Number of Transcripts

2

Number of Unique Polypeptides

2

Please see the JBrowse view of Dmel\CSN5 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry Q9XZ58)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Gene model reviewed during 5.47

Sequence Ontology: Class of Gene

protein_coding_gene

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

FBtr0083292

1196

327

FBtr0334535

1190

325

Additional Transcript Data and Comments

Reported size (kB)

1.198 (longest cDNA)

(Freilich et al., 1999)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

FBpp0082743

37.1

327

5.28

FBpp0306602

36.9

325

5.13

Polypeptides with Identical Sequences

None of the polypeptides share 100% sequence identity.

Additional Polypeptide Data and Comments

Reported size (kDa)

327 (aa)

(Freilich et al., 1999)

Comments

External Data

Subunit Structure (UniProtKB)

Component of the CSN complex, probably composed of CSN1b, alien/CSN2, CSN3, CSN4, CSN5, CSN6, CSN7 and CSN8. Interacts directly with CSN2. Also exists as monomeric form. Interacts via its MPN domain with Trc8.

(UniProt, Q9XZ58)

Domain

The JAMM motif is essential for the protease activity of the CSN complex resulting in deneddylation of cullins. It constitutes the catalytic center of the complex (By similarity).

(UniProt, Q9XZ58)

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\CSN5 using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

CSN5_1

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

3.02

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 3

Comment: maternally deposited

(Fisher et al., 2012)

embryonic stage 4 -- 6

(Fisher et al., 2012)

embryonic stage 7 -- 8

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 9 -- 10

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 13 -- 16

embryonic/larval midgut

(Fisher et al., 2012)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

• membrane

(Aradska et al., 2015)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

part_of COP9 signalosome

inferred from direct assay

(Freilich et al., 1999)

located_in cytoplasm

inferred from direct assay

(Harari-Steinberg et al., 2007, Suh et al., 2002)

located_in nuclear periphery

inferred from direct assay

(Harari-Steinberg et al., 2007)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\CSN5 in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\CSN5

View exonic expression by tissue for Dmel\CSN5

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0027053

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

42000/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0027053

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0027053

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0027053

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG14884

Flygut - An atlas of the Drosophila adult midgut

FBgn0027053

Images

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 20 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 25 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of CSN5

Transgenic constructs containing regulatory region of CSN5

Aberrations (Deficiencies and Duplications) ( 10 )

Inferred from experimentation ( 10 )

Gene disrupted in

(Freilich et al., 1999, Spradling et al., 1999, BDGP Project Members, 1994-1999)

(Ryder, 2004.4.26)

(Ryder, 2004.4.26)

(Sanchez-Herrero, 1996.3.7)

(Sanchez-Herrero, 1996.3.7)

(Suh et al., 2002)

Gene not disrupted in

(Sanchez-Herrero, 1996.3.7)

(Sanchez-Herrero, 1996.3.7)

(Suh et al., 2002)

(Sanchez-Herrero, 1996.3.7)

Inferred from location ( 0 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

CSN5^N

lethal (with CSN5^quo-2)

CSN5^quo-3

lethal (with CSN5^quo-3)

CSN5^quo-2

lethal | recessive

lethal | recessive | heat sensitive

CSN5^L4032

lethal - all die before end of larval stage

CSN5^N

lethal - all die before end of larval stage | recessive

CSN5^null

lethal - all die before end of pupal stage (with CSN5^null)

CSN5^quo-1

lethal - all die before end of pupal stage (with CSN5^quo-1)

CSN5^null

lethal - all die before end of pupal stage | recessive

lethal - all die before end of second instar larval stage (with CSN5^null)

CSN5^quo-2

lethal - all die before end of second instar larval stage (with CSN5^quo-2)

CSN5^null

partially lethal - majority die (with CSN5^null)

CSN5^quo-3

partially lethal - majority die (with CSN5^quo-3)

CSN5^null

viable (with CSN5^ex21)

CSN5^L4032

viable (with CSN5^L4032)

CSN5^ex21

Sterility

Allele

female sterile | germline clone | recessive

CSN5^null

female sterile germline-dependent | germline clone

CSN5^N

male sterile, with Scer\GAL4^bam.PU

CSN5^HMJ30047

Other Phenotypes

Allele

abnormal developmental rate

CSN5^null

abnormal size (with CSN5^null)

CSN5^quo-3

abnormal size (with CSN5^quo-3)

CSN5^null

chemical sensitive | larval stage

CSN5^null

decreased fecundity | male, with Scer\GAL4^bam.PU

CSN5^HMJ30047

decreased occurrence of cell division | adult stage | female | germline clone

CSN5^N

decreased size | male, with Scer\GAL4^bam.PU

CSN5^HMJ30047

increased cell number | oogenesis (with CSN5^ex13)

CSN5^L4032

increased cell number | oogenesis (with CSN5^ex21)

CSN5^L4032

increased cell number | oogenesis (with CSN5^L4032)

increased occurrence of cell division | germline clone | oogenesis

CSN5^L4032

some die during immature adult stage | recessive (with CSN5^quo-2)

CSN5^quo-3

some die during immature adult stage | recessive (with CSN5^quo-3)

CSN5^quo-2

some die during larval stage

CSN5^N

some die during larval stage | recessive

CSN5^null

some die during pupal stage (with CSN5^null)

CSN5^quo-1

some die during pupal stage (with CSN5^quo-1)

CSN5^null

some die during pupal stage | recessive

some die during second instar larval stage (with CSN5^null)

CSN5^quo-2

some die during second instar larval stage (with CSN5^quo-2)

CSN5^null

visible, with Scer\GAL4^dpp.PU

CSN5^NIG.14884R

visible | adult stage | somatic clone

CSN5^null

visible | somatic clone

CSN5^null

CSN5^Tag:MYC

Phenotype manifest in

Allele

cyst cell of ovary | germline clone

CSN5^N

dorsal appendage (with CSN5^ex21)

CSN5^L4032

dorsal appendage (with CSN5^L4032)

CSN5^ex21

dorsal appendage | germline clone

egg | germline clone

egg chorion | germline clone

CSN5^L4032

embryonic/larval optic lobe

CSN5^quo-2

eye | somatic clone

CSN5^null

female germline stem cell | germline clone

CSN5^N

fusome | germline clone | oogenesis

CSN5^L4032

CSN5^L4032

karyosome, with Scer\GAL4^{VP16.nanos.UTR}

CSN5^HMJ30047

lamina anlage glial cell

CSN5^quo-1

lamina anlage glial cell | somatic clone

CSN5^quo-1

CSN5^null

nurse cell (with CSN5^ex13)

CSN5^L4032

nurse cell (with CSN5^ex21)

CSN5^L4032

nurse cell (with CSN5^L4032)

nurse cell fusome | germline clone

CSN5^N

nurse cell spectrosome | germline clone

CSN5^quo-2

ovary | germline clone

photoreceptor cell R1 & axon | somatic clone

CSN5^quo-1

photoreceptor cell R2 & axon

CSN5^quo-1

photoreceptor cell R2 & axon (with Df(3R)RK6-3)

CSN5^quo-1

photoreceptor cell R2 & axon | somatic clone

CSN5^quo-1

photoreceptor cell R3 & axon

CSN5^quo-1

photoreceptor cell R3 & axon (with Df(3R)RK6-3)

CSN5^quo-1

photoreceptor cell R3 & axon | somatic clone

CSN5^quo-1

photoreceptor cell R4 & axon

CSN5^quo-1

photoreceptor cell R4 & axon (with Df(3R)RK6-3)

CSN5^quo-1

photoreceptor cell R4 & axon | somatic clone

CSN5^quo-1

photoreceptor cell R5 & axon

CSN5^quo-1

photoreceptor cell R5 & axon (with Df(3R)RK6-3)

CSN5^quo-1

photoreceptor cell R5 & axon | somatic clone

CSN5^quo-1

photoreceptor cell R6 & axon | somatic clone

CSN5^quo-1

primordial germ cell | germline clone

CSN5^L4032

scutellum, with Scer\GAL4^dpp.PU

CSN5^NIG.14884R

seminal vesicle, with Scer\GAL4^bam.PU

CSN5^HMJ30047

spermatozoon | absent, with Scer\GAL4^bam.PU

CSN5^HMJ30047

synaptonemal complex | oogenesis, with Scer\GAL4^{VP16.nanos.UTR}

CSN5^HMJ30047

testis, with Scer\GAL4^A3.eya

CSN5^JF03159

testis, with Scer\GAL4^c784

CSN5^JF03159

testis, with Scer\GAL4^NP1624

CSN5^JF03159

trichogen cell | increased number, with Scer\GAL4^dpp.PU

CSN5^NIG.14884R

trichogen cell | somatic clone | increased number

CSN5^null

wing (with CSN5^null)

CSN5^quo-3

wing (with CSN5^quo-3)

CSN5^null

wing vein | ectopic | somatic clone

CSN5^null

wing vein | somatic clone

CSN5^null

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (12)

14 of 14

Yes

Yes

2

3 of 14

No

No

2 of 14

No

Yes

2 of 14

No

No

2 of 14

No

No

2 of 14

No

Yes

2 of 14

No

No

2 of 14

No

No

1

2 of 14

No

No

1

1 of 14

No

No

1 of 14

No

No

1 of 14

No

No

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (13)

14 of 14

Yes

Yes

2 of 14

No

Yes

2 of 14

No

No

2 of 14

No

No

2 of 14

No

No

2 of 14

No

Yes

2 of 14

No

No

2 of 14

No

No

2 of 14

No

No

2 of 14

No

No

1 of 14

No

No

Rnor\LOC108348062

1 of 14

No

No

1 of 14

No

No

Mus musculus (laboratory mouse) (13)

13 of 14

Yes

Yes

3 of 14

No

No

2 of 14

No

Yes

2 of 14

No

No

2 of 14

No

No

2 of 14

No

No

2 of 14

No

Yes

2 of 14

No

No

2 of 14

No

No

2 of 14

No

No

1 of 14

No

No

1 of 14

No

Yes

1 of 14

No

No

Xenopus tropicalis (Western clawed frog) (11)

11 of 13

Yes

Yes

2 of 13

No

No

1 of 13

No

Yes

1 of 13

No

No

1 of 13

No

No

1 of 13

No

No

1 of 13

No

Yes

1 of 13

No

No

1 of 13

No

Yes

1 of 13

No

No

1 of 13

No

No

Danio rerio (Zebrafish) (13)

14 of 14

Yes

Yes

3 of 14

No

No

2 of 14

No

No

2 of 14

No

No

2 of 14

No

Yes

2 of 14

No

No

2 of 14

No

No

2 of 14

No

No

1 of 14

No

Yes

1 of 14

No

No

1 of 14

No

No

1 of 14

No

No

1 of 14

No

No

Caenorhabditis elegans (Nematode, roundworm) (7)

14 of 14

Yes

Yes

4 of 14

No

No

3 of 14

No

No

2 of 14

No

No

2 of 14

No

No

1 of 14

No

No

1 of 14

No

No

Anopheles gambiae (African malaria mosquito) (8)

Agam\AgaP_AGAP002880

12 of 12

Yes

Yes

Agam\AgaP_AGAP008617

3 of 12

No

No

Agam\AgaP_AGAP000960

2 of 12

No

No

Agam\AgaP_AGAP008156

2 of 12

No

No

Agam\AgaP_AGAP008858

2 of 12

No

No

Agam\AgaP_AGAP012135

2 of 12

No

No

Agam\EIF3F_ANOGA

2 of 12

No

No

Agam\EIF3H_ANOGA

1 of 12

No

No

Arabidopsis thaliana (thale-cress) (14)

13 of 13

Yes

Yes

13 of 13

Yes

Yes

3 of 13

No

No

2 of 13

No

No

2 of 13

No

No

2 of 13

No

No

2 of 13

No

Yes

2 of 13

No

Yes

2 of 13

No

No

2 of 13

No

No

2 of 13

No

No

2 of 13

No

No

2 of 13

No

No

1 of 13

No

No

Saccharomyces cerevisiae (Brewer's yeast) (3)

9 of 13

Yes

Yes

2 of 13

No

No

2 of 13

No

No

Schizosaccharomyces pombe (Fission yeast) (6)

11 of 12

Yes

Yes

2 of 12

No

No

2 of 12

No

No

Spom\SPBC4C3.07

2 of 12

No

No

2 of 12

No

No

Spom\SPAC821.05

1 of 12

No

No

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:CSN5. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (8)

5 of 13

3 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

COPS5; COP9 signalosome subunit 5

14 of 14

PSMD14; proteasome 26S subunit, non-ATPase 14

3 of 14

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Show neighbor-neighbor interactions:

Off

On

Show/hide secondary interactors
(data from AllianceMine provided by esyN)

Select Layout:

Force Directed

Circle

Concentric

Legend:

Protein

RNA

Selected Interactor(s)

Other Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

anti tag coimmunoprecipitation, anti tag western blot

(Pan et al., 2014)

anti tag coimmunoprecipitation, anti tag western blot

(Pan et al., 2014)

anti tag coimmunoprecipitation, anti tag western blot

(Huang et al., 2014)

two hybrid, pull down, western blot, autoradiography

(Lee et al., 2010, Gemmill et al., 2005, Gemmill et al., 2002)

Summary of Genetic Interactions

esyN Network Diagram

Show/hide secondary interactors
(data from AllianceMine provided by esyN)

esyN Network Key:

Suppression

Enhancement

Other Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

suppressible

(Doronkin et al., 2003)

suppressible

(Doronkin et al., 2002)

enhanceable

(Doronkin et al., 2003)

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

enhanceable

(Pan et al., 2014)

suppressible

(Doronkin et al., 2003)

enhanceable

(Doronkin et al., 2002)

enhanceable

(Doronkin et al., 2002)

suppressible

(Broemer et al., 2010)

suppressible

(Broemer et al., 2010)

enhanceable

(Muzzopappa and Wappner, 2005)

suppressible

(Gemmill et al., 2005)

External Data

Subunit Structure (UniProtKB)

Component of the CSN complex, probably composed of CSN1b, alien/CSN2, CSN3, CSN4, CSN5, CSN6, CSN7 and CSN8. Interacts directly with CSN2. Also exists as monomeric form. Interacts via its MPN domain with Trc8.

(UniProt, Q9XZ58 )

Linkouts

BioGRID - A database of protein and genetic interactions.

67045

DroID - A comprehensive database of gene and protein interactions.

FBgn0027053

MIST (genetic) - An integrated Molecular Interaction Database

42000

MIST (protein-protein) - An integrated Molecular Interaction Database

42000

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

External Data

Linkouts

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Genomic Location and Detailed Mapping Data

Chromosome (arm)

3R

Recombination map

3-58

Cytogenetic map

Sequence location

3R:16,470,099..16,471,406 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

89B17-89B17

Limits computationally determined from genome sequence between P{PZ}gish⁰⁴⁸⁹⁵&P{EP}EP3171 and P{lacW}CSN5^L4032

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

89D1-89D2

(Freilich et al., 1999, BDGP Project Members, 1994-1999)

89D1-89D2

(determined by in situ hybridisation)

(Spradling et al., 1999)

Experimentally Determined Recombination Data

Location

3-58

(FlyBase, 2016)

3-58.4

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (11)

Aberrations including deletions of this gene Aberrations including duplications of this gene

28732

y¹ v¹; P{TRiP.JF03159}attP2

42781

y¹ v¹; P{TRiP.GL01151}attP2

62970

y¹ v¹; P{TRiP.HMJ30047}attP40

62622

w¹¹¹⁸; PBac{IT.GAL4}CSN5^0076-G4/TM6B, Tb¹

10301

y¹ w¹¹¹⁸; P{lacW}CSN5^L4032/TM3, Ser¹

80178

y¹ sc^* v¹ sev²¹; P{TOE.GS02231}attP40

F001368

M{UAS-CSN5.ORF}ZH-86Fb

F001149

M{UAS-CSN5.ORF.3xHA}ZH-86Fb

111044

y^d2 w¹¹¹⁸ P{ey-FLP.N}2 P{5xglBS-lacZ.38-1}TPN1; P{neoFRT}82B P{lacW}CSN5^L4032/TM6B, P{Car20y}TPN1, Tb¹

HMJ30047

y¹ v¹; P{TRiP.HMJ30047}attP40/CyO

v330496

P{VSH330496}attP40

Genomic Clones (14)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (30)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

LD14392

Other clones

DPiM_LD14392

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0027053

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0027053

Antibody Information

Laboratory Generated Antibodies

polyclonal

(Freilich et al., 1999)

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

S2 cells are treated with dsRNA made from templates generated with primers directed against this gene to assess effect on degradation of dup and geminin genes.

(Higa et al., 2003)

Homozygous mutant clones in the female germline disrupt both the dorsoventral and anterioposterior axes of the oocyte as a result of decreased grk protein.

(Doronkin et al., 2002)

CSN5 is required in R cells to induce lamina glial cell migration.

(Suh et al., 2002)

COP9 signalosome is essential for development.

(Freilich et al., 1999)

Relationship to Other Genes

Source for database merge of

Source for merge of: CH5 l(3)L4032

(Freilich et al., 1999)

Source for merge of: CSN5 l(3)89Cf

(Doronkin et al., 2003)

Additional comments

Nomenclature History

Source for database identify of

Nomenclature comments

Etymology

Synonyms and Secondary IDs (28)

Reported As

Symbol Synonym

BcDNA.LD14392

(Tsang, 1999.3.2)

BcDNA:LD14392

CG14884

(Páhi et al., 2022, Meadows, 2018.4.4, Yang et al., 2014, Schertel et al., 2013, Tsou et al., 2012, Zhang et al., 2012, Ni et al., 2010.12.1, Perkins et al., 2009, Gandhi et al., 2007)

CH5

CSN-5

(Gemmill et al., 2002)

CSN5

(Barbaste et al., 2023, Heigwer et al., 2023, Páhi et al., 2022, Zhao et al., 2022, Zong et al., 2022, Badmos et al., 2021, Barbosa et al., 2021, Evans et al., 2021, Lin et al., 2020, Cho et al., 2018, Kim et al., 2017, Stobdan et al., 2017, Suisse et al., 2017, Transgenic RNAi Project members, 2017-, Clandinin and Owens, 2016-, Appel et al., 2015, Lu et al., 2015, Qian et al., 2015, Huang et al., 2014, Kim and Choe, 2014, Singer et al., 2014, Yan et al., 2014, Bischof et al., 2013, McPhee et al., 2013, Schertel et al., 2013, Joyce et al., 2012, Tsou et al., 2012, Axelsson et al., 2011, Guest et al., 2011, Kondo and Perrimon, 2011, White et al., 2011, Wu et al., 2011, Broemer et al., 2010, Kim et al., 2010, Djagaeva and Doronkin, 2009, Dworkin et al., 2009, Knowles et al., 2009, Mummery-Widmer et al., 2009, Chan et al., 2008, Chan et al., 2008, Oren-Giladi et al., 2008, Rencus-Lazar et al., 2008, Buckley et al., 2007, Ullah et al., 2007, Buckley et al., 2006, Higa et al., 2006, Gemmill et al., 2005, Wu et al., 2005, Schwechheimer, 2004, Stanyon et al., 2004)

Csn5

(Pan et al., 2014, Lee et al., 2010, Wei et al., 2008, Ullah et al., 2007, Cope et al., 2002, Oron et al., 2001)

DCH5

(Segal et al., 2000, Freilich, 1999.2.17, Segal et al., 1999)

Dch5

(Wei and Deng, 2003, Freilich et al., 1999)

DmCSN5

(Gao et al., 2023)

JAB1

(Hadjieconomou et al., 2011, Gemmill et al., 2002)

JAB1/CSN5

(Suh et al., 2002)

Jab1

(Pandey et al., 2018, Cope et al., 2002, Doronkin et al., 2001)

JadBp

(Spradling et al., 1999)

Q9XZ58

(Clague et al., 2013)

csn5

(Lu et al., 2015, Lee et al., 2010, Oren-Giladi et al., 2008, Rencus-Lazar et al., 2008, Harari-Steinberg et al., 2007, Oron et al., 2007, Ullah et al., 2007, Harari-Steinberg et al., 2005, Oron et al., 2005, Park et al., 2004, Wei and Deng, 2003, Oron et al., 2002)

dch5

(Freilich et al., 1999)

jab1/csn5

(Chotard and Salecker, 2004, Tayler and Garrity, 2003)

l(3)89Cf

(Sanchez-Herrero, 1996.3.7)

l(3)L4032

(Beaton, 2000.1.31, BDGP Project Members, 1994-1999)

l(3)M203

(Sanchez-Herrero, 1998.8.30)

quo

(Suh et al., 2002)

Name Synonyms

COP9 complex homolog subunit 5

(Freilich, 1999.2.17)

COP9 complex subunit 5

(Davis et al., 2011)

COP9 signalosome subunit 5

Drosophila COP9 signalosome homolog 5

(Freilich et al., 1999)

JUN activation domain-binding protein-1

(Doronkin et al., 2001)

Jun activation domain binding protein

(Spradling et al., 1999)

Secondary FlyBase IDs

FBgn0016027
FBgn0024551

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 47 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

42000

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

Q9XZ58

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

Q9XZ58

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

A0A0B4KHM2

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

Q9XZ58

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0027053

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

42000/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0027053

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0027053

FlyMine - An integrated database for Drosophila genomics

FBgn0027053

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG14884

MARRVEL_MODEL - MARRVEL (model organism gene)

42000

Linkouts

BioGRID - A database of protein and genetic interactions.

67045

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0027053

DroID - A comprehensive database of gene and protein interactions.

FBgn0027053

DRSC - Results frm RNAi screens

FBgn0027053

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

CSN5_1

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0027053

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0027053

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG14884

Flygut - An atlas of the Drosophila adult midgut

FBgn0027053

FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.

FBgn0027053

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC011816

Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution

Interactive Fly

MIST (genetic) - An integrated Molecular Interaction Database

42000

MIST (protein-protein) - An integrated Molecular Interaction Database

42000

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

References (130)