FlyBase Gene Report: Dmel\pygo

General Information

Symbol

Dmel\pygo

Species

D. melanogaster

Name

pygopus

Annotation Symbol

CG11518

Feature Type

protein_coding_gene

FlyBase ID

FBgn0043900

Gene Model Status

Current

Stock Availability

11 publicly available

Gene Summary

pygopus (pygo) encodes a key nuclear component of the Wnt signaling pathway. It facilitates the recruitment of the product of arm to Wnt-responsive genes by interacting with the transcription cofactor encoded by Chi. It also binds the product of lgs and the methylated tail of the product of His3. Together, they associate with the product of arm in a process that may function as a transition from gene silence to Wnt-induced transcription. [Date last reviewed: 2019-03-14] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
Pathway
Gene Group
UniProtKB
Interactive Fly

Also Known As

gam, BEST:LD21971 , dPygo

Key Links

Genomic Location

Cytogenetic map

100C6-100C6

Sequence location

3R:31,575,835..31,580,699 [-]

Recombination map

3-102

RefSeq locus

NT_033777 REGION:31575835..31580699

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (14 terms)

Molecular Function (2 terms)

Terms Based on Experimental Evidence (2 terms)

CV Term

Evidence

References

enables methylated histone binding

inferred from direct assay

(Fiedler et al., 2008)

enables transcription coactivator activity

inferred from genetic interaction with FLYBASE:arm; FB:FBgn0000117

(Hoffmans et al., 2005, Thompson, 2004)

inferred from direct assay

(Thompson, 2004)

inferred from physical interaction with UniProtKB:P18824,UniProtKB:Q961D9

(Thompson, 2004)

Terms Based on Predictions or Assertions (0 terms)

Biological Process (10 terms)

Terms Based on Experimental Evidence (10 terms)

CV Term

Evidence

References

involved_in canonical Wnt signaling pathway

inferred from mutant phenotype

(Carrera et al., 2008, Thompson, 2004, Belenkaya et al., 2002, Kramps et al., 2002, Parker et al., 2002)

inferred from genetic interaction with FLYBASE:arm; FB:FBgn0000117

(Hoffmans et al., 2005, Parker et al., 2002, Thompson et al., 2002)

inferred from genetic interaction with FLYBASE:Axn; FB:FBgn0026597

(Parker et al., 2002)

inferred from genetic interaction with FLYBASE:wg; FB:FBgn0284084

(Parker et al., 2002)

inferred from direct assay

(Bhambhani et al., 2011)

inferred from genetic interaction with FLYBASE:Apc2; FB:FBgn0026598

(Kramps et al., 2002)

involved_in chitin-based larval cuticle pattern formation

inferred from mutant phenotype

(Thompson, 2004)

involved_in delamination

inferred from mutant phenotype

(Kiger et al., 2007)

involved_in embryonic pattern specification

inferred from mutant phenotype

(Fiedler et al., 2015, Thompson et al., 2002)

involved_in eye-antennal disc development

inferred from mutant phenotype

(Lin et al., 2004)

involved_in imaginal disc-derived wing expansion

inferred from mutant phenotype

(Kiger et al., 2007)

involved_in positive regulation of transcription by RNA polymerase II

inferred from mutant phenotype

(Thompson et al., 2002)

inferred from direct assay

(Thompson, 2004)

inferred from genetic interaction with FLYBASE:arm; FB:FBgn0000117

(Hoffmans et al., 2005)

involved_in regulation of transcription by RNA polymerase II

inferred from physical interaction with UniProtKB:P18824

(Kramps et al., 2002)

inferred from physical interaction with UniProtKB:Q961D9

(Kramps et al., 2002)

involved_in segment polarity determination

inferred from mutant phenotype

(Thompson, 2004)

inferred from mutant phenotype

(Kramps et al., 2002)

involved_in wing disc morphogenesis

inferred from mutant phenotype

(Kiger et al., 2007)

Terms Based on Predictions or Assertions (0 terms)

Cellular Component (2 terms)

Terms Based on Experimental Evidence (2 terms)

CV Term

Evidence

References

part_of beta-catenin-TCF complex

inferred from direct assay

(Thompson, 2004)

located_in nucleus

inferred from direct assay

(Hoffmans et al., 2005, Thompson, 2004, Thompson et al., 2002)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

located_in nucleus

non-traceable author statement

(Kramps et al., 2002)

Gene Group (FlyBase)

WNT ENHANCEOSOME

Pathway (FlyBase)

Wnt-TCF Signaling Pathway Core Components

Protein Family (UniProt)

Protein Signatures (InterPro)

Summaries

Gene Snapshot

Pathway (FlyBase)

Wnt-TCF Signaling Pathway Core Components -: The canonical Wnt signaling pathway is initiated by the binding of a Wnt ligand to a frizzled family receptor on the cell surface. Activation of the pathway leads to the inhibition of cytoplasmic β-catenin (arm) degradation and its subsequent accumulation in the nucleus, where it regulates the transcription of target genes. (Adapted from FBrf0218499 and FBrf0223299).

Gene Group (FlyBase)

WNT ENHANCEOSOME -: The Wnt enhanceosome complex binds to Wnt/wg-enhancer elements via the TCF/LEF protein, pan. In the absence nuclear β-catenin (arm), Wnt/wg-responsive genes are silenced by the repressor gro binding to the enhanceosome. Activation of the canonical Wnt signaling pathway leads to arm accumulation in the nucleus, incorporation into the enhanceosome and the stimulation of transcription. (Adapted from FBrf0235074 and FBrf0229654).

Protein Function (UniProtKB)

Involved in signal transduction through the Wnt pathway.

(UniProt, Q9V9W8)

Summary (Interactive Fly)

nuclear PHD-finger protein - a core component of the Wingless signaling pathway that acts downstream of or at the level of Pangolin - a complex of Armadillo, Legless, and Pygopus coactivates dTCF to activate Wingless target genes

Interactive Fly

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\pygo for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry Q9V9W8)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

PDB - An information portal to biological macromolecular structures

3ZPV

Comments on Gene Model

Gene model reviewed during 5.44

Gene model reviewed during 5.53

Sequence Ontology: Class of Gene

nuclear_gene

protein_coding_gene

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

pygo-RA

FBtr0085806

NM_143615

4507

815

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

pygo-PA

FBpp0085167

80.5

815

8.38

Q9V9W8

NP_651872

AAF57161

Polypeptides with Identical Sequences

There is only one protein coding transcript and one polypeptide associated with this gene

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Subunit Structure (UniProtKB)

Binds to BCL9 via the PHD-type zinc finger motif, and thereby becomes part of the nuclear ARM/PAN complex.

(UniProt, Q9V9W8)

Crossreferences

InterPro - A database of protein families, domains and functional sites

PDB - An information portal to biological macromolecular structures

3ZPV

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Q9V9W8

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\pygo using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

pygo_1

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

0.67

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage

organism

(Parker et al., 2002)

larval stage

organism

(Parker et al., 2002)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

immunolocalization

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

cardioblast

(Tang et al., 2013)

Additional Descriptive Data

Anti-pygo staining shows nuclear localization in both small and large myocardial (cardioblast) nuclei.

(Tang et al., 2013)

Marker for

Subcellular Localization

CV Term

Evidence

References

part_of beta-catenin-TCF complex

inferred from direct assay

(Thompson, 2004)

located_in nucleus

inferred from direct assay

(Hoffmans et al., 2005, Thompson, 2004, Thompson et al., 2002)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\pygo in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\pygo

View exonic expression by tissue for Dmel\pygo

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

43718/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0043900

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0043900

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0043900

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG11518

Flygut - An atlas of the Drosophila adult midgut

FBgn0043900

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 17 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 26 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of pygo

Transgenic constructs containing regulatory region of pygo

Aberrations (Deficiencies and Duplications) ( 6 )

Inferred from experimentation ( 6 )

Gene not disrupted in

Df(3R)awd-KRB

(Cook, 2006.1.31)

Df(3R)tll-e

(Cook, 2006.1.31)

Df(3R)tll-g

(Cook, 2006.1.31)

Gene partially disrupted in

Df(3R)γCOP⁵⁷⁷

(Grieder et al., 2008)

Df(3R)γCOP⁶⁷⁷

(Grieder et al., 2008)

Df(3R)pygo^11-3

(Grieder et al., 2008)

Inferred from location ( 0 )

Dp(3;2)GV-CH321-70F21

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal, with Scer\GAL4^insc-Mz1407

pygo^GD1516

lethal (with pygo^S28)

pygo^S123

lethal (with pygo^S123)

pygo^S28

lethal | embryonic stage | germline clone | maternal effect

pygo¹⁰

lethal | germline clone | recessive

pygo¹³⁰

lethal | larval stage | recessive

pygo^unspecified

lethal | pupal stage | recessive

lethal - all die before end of pupal stage

pygo^EP1076

lethal - all die before end of pupal stage (with pygo⁹)

pygo¹⁰

lethal - all die before end of pupal stage (with pygo¹⁰)

pygo⁹

lethal - all die during embryonic stage | maternal effect | recessive

lethal - all die during larval stage, with Scer\GAL4^Mef2.PR

pygo^GD1516

partially lethal - majority die, with Scer\GAL4^pnr-MD237

pygo^GD1516

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

pygo^GD1516

viable, with Scer\GAL4^tin.CΔ4

pygo^GD1516

Other Phenotypes

Allele

abnormal cell growth | somatic clone

pygo^S28

abnormal cell shape | pupal stage | somatic clone

pygo^S123

abnormal cell shape | somatic clone | third instar larval stage

pygo^S123

abnormal neuroanatomy | larval stage, with Scer\GAL4^insc-Mz1407

pygo^GD1516

abnormal size | larval stage | somatic clone

pygo^S123

decreased cell death | somatic clone

pygo¹⁰

increased cell death | somatic clone

pygo^S28

increased cell number | adult stage | cell non-autonomous | somatic clone

pygo^S123

increased cell number | adult stage | somatic clone

pygo^S123

pair rule phenotype | maternal effect

segment polarity phenotype | germline clone | recessive

pygo¹³⁰

segment polarity phenotype | maternal effect

segment polarity phenotype | rescuable maternal effect

pygo^F107

some die during embryonic stage | recessive | maternal effect | recessive

some die during pupal stage

pygo^EP1076

some die during pupal stage, with Scer\GAL4^pnr-MD237

pygo^GD1516

some die during pupal stage (with pygo⁹)

pygo¹⁰

some die during pupal stage (with pygo¹⁰)

pygo⁹

visible, with Scer\GAL4^en-e16E

pygo^EP1076

visible, with Scer\GAL4^ey.PH

pygo^EP1076

visible, with Scer\GAL4^pnr-MD237

pygo^GD1516

visible, with Scer\GAL4^ptc-559.1

pygo^UAS.cPa

visible, with Scer\GAL4^sca-537.4

pygo^EP1076

visible | recessive | somatic clone

pygo^F66

visible | somatic clone

pygo^S28

wild-type

pygo^αTub84B.PK

Phenotype manifest in

Allele

adult cuticle

pygo^EP1076

adult midgut | cell non-autonomous | somatic clone

pygo^S123

adult midgut | somatic clone

pygo^S123

cone cell | pupal stage | somatic clone

pygo^S123

cone cell | somatic clone | third instar larval stage

pygo^S123

cuticle

pygo¹⁰

denticle | ectopic

pygo^S28

denticle | ectopic | germline clone | rescuable maternal effect

pygo^S123

denticle field | embryonic stage | maternal effect

pygo^S123

dorsocentral bristle, with Scer\GAL4^sca-537.4

pygo^EP1076

embryo | maternal effect

embryo | rescuable maternal effect

pygo^F107

embryonic/first instar larval cuticle

pygo^S28

embryonic/first instar larval cuticle | germline clone

embryonic/first instar larval cuticle | germline clone | rescuable maternal effect

pygo^S123

embryonic/first instar larval cuticle | maternal effect

embryonic/first instar larval cuticle | rescuable maternal effect

pygo^F107

embryonic epidermis

pygo¹⁰

embryonic midgut constriction 2 | germline clone

eye, with Scer\GAL4^ey.PH

pygo^EP1076

eye photoreceptor cell | P-stage | ectopic | somatic clone

pygo¹⁰

ganglion mother cell | larval stage, with Scer\GAL4^insc-Mz1407

pygo^GD1516

haltere disc | larval stage | somatic clone

pygo^S123

imaginal disc

pygo¹⁰

larval neuroblast | larval stage, with Scer\GAL4^insc-Mz1407

pygo^GD1516

larval RP2 motor neuron

pygo^unspecified

leg

pygo^EP1076

leg | distal | somatic clone

pygo^S28

mesothoracic tergum, with Scer\GAL4^pnr-MD237

pygo^GD1516

ommatidium | P-stage | ectopic | somatic clone

pygo¹⁰

photoreceptor neuron | ectopic | somatic clone

pygo¹⁰

sarcomere, with Scer\GAL4^Mef2.PR

pygo^GD1516

scutellar bristle, with Scer\GAL4^sca-537.4

pygo^EP1076

sex comb

pygo^EP1076

trichogen cell, with Scer\GAL4^pnr-MD237

pygo^GD1516

ventral denticle belt

pygo¹⁰

ventral denticle belt | maternal effect

ventral denticle belt | rescuable maternal effect

pygo^F107

wing, with Scer\GAL4^30A

pygo^UAS.cPa

wing, with Scer\GAL4^en-e16E

pygo^EP1076

wing | somatic clone

wing disc | somatic clone

pygo^S28

wing margin, with Scer\GAL4^ptc-559.1

pygo^UAS.cPa

wing margin | somatic clone

wing margin bristle | cell non-autonomous | ectopic | somatic clone

pygo^F66

Z disc, with Scer\GAL4^Mef2.PR

pygo^GD1516

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (2)

Hsap\PYGO2

6 of 14

Yes

Hsap\PYGO1

5 of 14

Yes

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (2)

Rnor\Pygo2

6 of 14

Yes

Rnor\Pygo1

5 of 14

Yes

Mus musculus (laboratory mouse) (2)

Mmus\Pygo2

5 of 14

Yes

Mmus\Pygo1

4 of 14

Yes

Xenopus tropicalis (Western clawed frog) (1)

Xtro\pygo2

2 of 13

Yes

Danio rerio (Zebrafish) (2)

Drer\pygo2

7 of 14

Yes

Drer\pygo1

4 of 14

Yes

Caenorhabditis elegans (Nematode, roundworm) (1)

Cele\C02B10.5

2 of 14

Yes

Anopheles gambiae (African malaria mosquito) (1)

Agam\AgaP_AGAP013538

8 of 12

Yes

Arabidopsis thaliana (thale-cress) (0)

Saccharomyces cerevisiae (Brewer's yeast) (1)

Scer\DEF1

1 of 13

Yes

Schizosaccharomyces pombe (Fission yeast) (1)

Spom\def1

1 of 12

Yes

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:pygo. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (1)

lgs

1 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

cardiac dysfunction (postulated), PYGO-related

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Allele

Disease

Evidence

References

pygo^unspecified

model of heart conduction disease

CEA

(Ocorr et al., 2017)

CEA with sei¹

(Ocorr et al., 2017)

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Allele

Disease

Interaction

References

pygo^KK108114

ameliorates gastrointestinal system cancer

modeled by Apc2^g10, Apc^Q8

(Wang et al., 2013)

ameliorates retinitis pigmentosa 4

modeled by ninaE^P37H.UAS

(Park et al., 2023)

ameliorates Huntington's disease

modeled by Hsap\HTT^Q128.UAS

(Martin et al., 2021)

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

PYGO2; pygopus family PHD finger 2

6 of 14

606903

PYGO1; pygopus family PHD finger 1

5 of 14

606902

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Dmel gene

Ortholog showing functional complementation

Supporting References

pygo

Hsap\PYGO1

(Kramps et al., 2002)

pygo

Hsap\PYGO2

(Kramps et al., 2002)

Interactions

Summary of Physical Interactions

esyN Network Diagram

Show neighbor-neighbor interactions:

Off

Show/hide secondary interactors
(data from AllianceMine provided by esyN)

Select Layout:

Force Directed

Circle

Concentric

Legend:

Protein

RNA

Selected Interactor(s)

Other Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

pygo - Chi

nuclear magnetic resonance

(Fiedler et al., 2015)

pygo - His3

isothermal titration calorimetry, predetermined participant, pull down, western blot, nuclear magnetic resonance

(Miller et al., 2013, Kessler et al., 2009, Fiedler et al., 2008)

pygo - Taf4

pull down, western blot

(Wright and Tjian, 2009)

pygo - arm

anti tag coimmunoprecipitation, western blot, anti tag western blot

(Thompson, 2004, Belenkaya et al., 2002)

pygo - ebd1

anti tag coimmunoprecipitation, anti tag western blot, pull down

(Benchabane et al., 2011)

pygo - lgs

anti tag coimmunoprecipitation, anti tag western blot, x-ray crystallography, predetermined participant, pull down, autoradiography, two hybrid, western blot

(Miller et al., 2013, Kessler et al., 2009, Hoffmans et al., 2005, Thompson, 2004, Townsley et al., 2004, Kramps et al., 2002)

pygo - skd

anti bait coimmunoprecipitation, anti tag western blot

(Carrera et al., 2008)

Summary of Genetic Interactions

esyN Network Diagram

Show/hide secondary interactors
(data from AllianceMine provided by esyN)

esyN Network Key:

Suppression

Enhancement

Other Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

pygo

suppressible

arm

(Townsley et al., 2004)

pygo

suppressible

CtBP

(Bhambhani et al., 2011)

pygo

suppressible

CycE

(Tseng and Hariharan, 2002)

pygo

suppressible

lgs::pygo

(Thompson, 2004, Kramps et al., 2002)

pygo

suppressible

pan::pygo

(Thompson, 2004)

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Apc2|Apc

suppressible

pygo

(Wang et al., 2013)

arm

suppressible

pygo

(van Tienen et al., 2017, Parker et al., 2002, Thompson et al., 2002)

Axn

suppressible

pygo

(Zhang et al., 2017, Parker et al., 2002)

Chi

suppressible

pygo

(van Tienen et al., 2017)

suppressible

pygo

(Singh et al., 2005)

suppressible

pygo

(Cordero and Cagan, 2010)

ninaE

suppressible

pygo

(Park et al., 2023)

suppressible

pygo

(Kramps et al., 2002, Parker et al., 2002)

External Data

Subunit Structure (UniProtKB)

Binds to BCL9 via the PHD-type zinc finger motif, and thereby becomes part of the nuclear ARM/PAN complex.

(UniProt, Q9V9W8 )

Linkouts

BioGRID - A database of protein and genetic interactions.

68561

DroID - A comprehensive database of gene and protein interactions.

FBgn0043900

MIST (genetic) - An integrated Molecular Interaction Database

43718

MIST (protein-protein) - An integrated Molecular Interaction Database

43718

Pathways

Signaling Pathways (FlyBase)

Wnt-TCF Signaling Pathway Core Components -: The canonical Wnt signaling pathway is initiated by the binding of a Wnt ligand to a frizzled family receptor on the cell surface. Activation of the pathway leads to the inhibition of cytoplasmic β-catenin (arm) degradation and its subsequent accumulation in the nucleus, where it regulates the transcription of target genes. (Adapted from FBrf0218499 and FBrf0223299).

Metabolic Pathways

External Data

Linkouts

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

SignaLink - A signaling pathway resource with multi-layered regulatory networks.

Q9V9W8

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

3-102

Cytogenetic map

100C6-100C6

Sequence location

3R:31,575,835..31,580,699 [-]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

100C6-100C6

Limits computationally determined from genome sequence between P{PZ}l(3)rM731^rM731 and P{EP}pygo^EP1076

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

100C6-100C7

(Kramps, 2001.12.11)

100D-100D

(Parker, 2002.1.24)

100C6-100C7

(determined by in situ hybridisation)

(Abdelilah-Seyfried et al., 2000)

Experimentally Determined Recombination Data

Location

3-102

(FlyBase, 2016, Thompson et al., 2002)

3-99.1

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (11)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

29049

w^*; P{UAS-pygo.HA}3/TM6B, Tb¹

29720

w^*; pygo¹⁰/TM6B, Tb¹

80251

y¹ sc^* v¹ sev²¹; P{TOE.GS02793}attP40

93505

w^*; TI{CRIMIC.TG4.2}pygo^{CR60177-TG4.2}/TM3, Sb¹

7208

w^*; P{neoFRT}82B pygo^S28/TM3, Sb¹ Ser¹

38208

y¹ sc^* v¹ sev²¹; P{TRiP.GL00647}attP40

57469

y¹ sc^* v¹ sev²¹; P{TRiP.HMC04780}attP40

7209

w^*; P{neoFRT}82B pygo^S123/TM6B, P{Ubi-GFP.S65T}PAD2, Tb¹

Kyoto

123912

w¹¹¹⁸; P{RS3}pygo^CB-6097-3

VDRC

v100724

P{KK108114}VIE-260B

v19692

w¹¹¹⁸; P{GD1516}v19692

Genomic Clones (19)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (59)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

LD18280

Other clones

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0043900

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0043900

Antibody Information

Laboratory Generated Antibodies

polyclonal

(de la Roche and Bienz, 2007)

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

dsRNA made from templates generated with primers directed against this gene.

(Fang et al., 2006, Hoffmans et al., 2005)

dsRNA has been made from templates generated with primers directed against this gene. RNAi of pygo causes a reduction in the arborization of ddaD and ddaE neurons. There is a severe reduction in primary branch outgrowth, lateral branching and lateral branch length. Additionally, there is also inappropriate dendritic routing of ddaE neurons.

(Parrish et al., 2006)

pygo is necessary for apoptosis of excess photoreceptors at the periphery of the developing eye during pupal stages.

(Lin et al., 2004)

In order to activate pan protein, arm protein must enter the nucleus and form a complex with lgs and pygo proteins.

(Thompson, 2004)

Null mutations have been isolated that affect the epidermis, denticle belts, antenna, legs and wings and are recessive viable.

(Cadigan, 2002.1.24)

pygo and lgs are required for wg signal transduction at the level of nuclear arm.

(Kramps et al., 2002)

pygo is required for wg signalling.

(Parker et al., 2002)

Identification: in a screen to identify P{EP} lines that suppress the small eye phenotype caused by overexpression of wg when coexpressed in the eye.

(Cadigan et al., 2001)

pygo inhibits wg signalling when overexpressed and when reduced in activity.

(Cadigan et al., 2001)

Identification: transcription unit identified during molecular analysis of the rod genomic region.

(Scaerou et al., 1999)

Relationship to Other Genes

Source for database merge of

Source for merge of: BEST:LD21971 CG11518

(Bayraktaroglu, 2000.11.2)

Source for merge of: gam BEST:LD21971

(Cadigan, 2002.1.24)

Source for merge of: pygo anon-WO02077023.1

(FlyBase, 1996-)

Additional comments

FlyBase curator comment: "EP3408" overexpression phenotype stated to be due to its effect on pygo but orientation of the P{EP} element suggests otherwise.

(Singh et al., 2005)

Source for merge of pygo anon-WO02077023.1 was sequence comparison ( date:051113 ).

(FlyBase, 1996-)

Nomenclature History

Source for database identify of

Source for identity of: pygo gam

(Cadigan et al., 2002.3.13)

Nomenclature comments

Etymology

A pygopus is a legless lizard with scaly skin.

(Kramps et al., 2002)

Synonyms and Secondary IDs (15)

Reported As

Symbol Synonym

BEST:LD21971

(Abdelilah-Seyfried et al., 2000)

CG11518

(Hosono et al., 2015, Ni et al., 2010.12.1, Keleman et al., 2009.8.5, Bienz, 2006, Tseng and Hariharan, 2002)

Dm Pygo

(Konikoff et al., 2010)

Pygo

(Cannon and Bodmer, 2016, Jenny and Basler, 2014, Mbodj et al., 2013, Benchabane et al., 2011, Benchabane et al., 2011, Bhambhani et al., 2011, Buechling et al., 2011, Wright and Tjian, 2009, Carrera et al., 2008, Stadeli, 2006, Parker and Cadigan, 2005)

anon-WO02077023.1

dPygo

(Shi et al., 2020, Kessler et al., 2009)

gam

(Cadigan, 2002.1.24, Kramps et al., 2002, Cadigan et al., 2001)

pNB5.2

(Scaerou et al., 1999)

pyg

(Parrish et al., 2006)

pygo

(Sood et al., 2024, Chen et al., 2022, Ecovoiu et al., 2022, López-Varea et al., 2021, Martin et al., 2021, Bosch et al., 2020, Tian et al., 2019, Wang et al., 2019, Billmann et al., 2018, Gene Disruption Project members, 2018-, Ocorr et al., 2017, Transgenic RNAi Project members, 2017-, van Tienen et al., 2017, Zhu et al., 2017, Jenny and Basler, 2016, Tian et al., 2016, Wang et al., 2016, Fiedler et al., 2015, Luo et al., 2015, Gersten et al., 2014, Tang et al., 2014, Miller et al., 2013, Shen et al., 2013, Tang et al., 2013, Wang et al., 2013, Japanese National Institute of Genetics, 2012.5.21, Toku et al., 2011, Cordero and Cagan, 2010, Kuwamura et al., 2010, Kessler et al., 2009, Benchabane et al., 2008, Fiedler et al., 2008, Grieder et al., 2008, Lebreton et al., 2008, Mieszczanek et al., 2008, Parker et al., 2008, DasGupta et al., 2007, de la Roche and Bienz, 2007, Kiger et al., 2007, Bienz, 2006, Fang et al., 2006, Jordan et al., 2006, Joulia et al., 2006, Ma et al., 2006, Mosimann et al., 2006, DasGupta et al., 2005, Jordan et al., 2005, Staedeli and Basler, 2005, Thompson, 2004)

pypo

(Furlong, 2005)

Name Synonyms

Pygopus

(Ahmed-de-Prado and Baonza, 2018, Cannon and Bodmer, 2016, Tian et al., 2016, Jenny and Basler, 2014, Bhambhani et al., 2011, Kessler et al., 2009, Wright and Tjian, 2009, Carrera et al., 2008, Fiedler et al., 2008, de la Roche and Bienz, 2007, Tountas and Fortini, 2007, Bienz, 2006, Clevers, 2006, He and Axelrod, 2006, Staedeli and Basler, 2005)

gammy legs

(Cadigan et al., 2001)

pygopous

(Grieder et al., 2008)

pygopus

(Tang et al., 2013, Cordero and Cagan, 2010, Lebreton et al., 2008, Parker et al., 2008, Pfleger et al., 2007, Budde, 2006, Fang et al., 2006, Ma et al., 2006, Parrish et al., 2006, Jordan et al., 2005, Cadigan et al., 2002.3.13, Parker, 2002.1.24, Kramps, 2001.12.11)

Secondary FlyBase IDs

FBgn0028951
FBgn0039853
FBgn0064198

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 50 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

43718

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

Q9V9W8

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

Q9V9W8

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

Q9V9W8

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

43718/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0043900

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0043900

FlyMine - An integrated database for Drosophila genomics

FBgn0043900

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG11518

MARRVEL_MODEL - MARRVEL (model organism gene)

43718

PDB - An information portal to biological macromolecular structures

3ZPV

Linkouts

BioGRID - A database of protein and genetic interactions.

68561

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0043900

DroID - A comprehensive database of gene and protein interactions.

FBgn0043900

DRSC - Results frm RNAi screens

FBgn0043900

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

pygo_1

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0043900

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0043900

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG11518

Flygut - An atlas of the Drosophila adult midgut

FBgn0043900

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC004669

Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution

Interactive Fly

MIST (genetic) - An integrated Molecular Interaction Database

43718

MIST (protein-protein) - An integrated Molecular Interaction Database

43718

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

SignaLink - A signaling pathway resource with multi-layered regulatory networks.

Q9V9W8

References (138)

The following external sites may use different assemblies or annotations than FlyBase.

0.67

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