Human Disease Model Report: cancer, multiple, NOTCH-PI3K-AKT-related

General Information

Name

cancer, multiple, NOTCH-PI3K-AKT-related

FlyBase ID

FBhh0000937

Disease Ontology Term

cancer

Parent Disease

OMIM

Overview

This report describes Drosophila models of cancer that combine mutations that result in overexpression of the Notch signaling pathway with misexpression of various genes within the PI3K/AKT pathway. Targeted overexpression of the Notch pathway is achieved by driving overexpression of the Notch ligand Delta (Dl); overexpression of Dl in the adult eye results in a mild overgrowth phenotype. In combination with increased expression of Akt, hyperproliferation in observed; in combination with increased expression of Pi3K92E, invasive and metastatic growth is observed. Overexpression of Dl in combination with increased expression of Pten results suppression of overgrowth phenotypes; when combined with knockdown of Pten effected by RNAi, a dramatic increase in hyperproliferation results. These systems have been used to assess the efficacy of antitumorigenic drugs that target inflammatory pathways.

In human, there are 4 genes that encode NOTCH family transmembrane receptor proteins (NOTCH1-NOTCH4); there are 2 genes similar to the Drosophila Notch ligand Delta (DLL1 and DLL4) and 2 genes similar to the Drosophila Notch ligand Serrate (JAG1 and JAG2). For PI3K-AKT signaling pathway genes, Dmel\Pi3K92E is orthologous to human PIK3C kinase catalytic subunit genes; Dmel\ Pten is orthologous to human PTEN phosphatase, a negative regulator of the PI3K/AKT pathway; Dmel\Pdk1 is orthologous to human PDPK1 kinase; and Dmel\Akt is orthologous to human AKT kinase genes.

[updated Dec. 2018 by FlyBase; FBrf0222196]

Disease Summary Information

Disease Summary: cancer, multiple, NOTCH-PI3K-AKT-related

OMIM report

Human gene(s) implicated

Symptoms and phenotype

Genetics

Cellular phenotype and pathology

Molecular information

External links

Disease synonyms

cancer, multiple, Akt/Notch signaling

Notch-PI3K/Akt cooperative oncogenesis

Search term: metastatic phenotype(s)

Related Diseases

Related human health report(s)

cancer, multiple, Notch signaling pathway

cancer, multiple, PI3K-AKT signaling pathway

Ortholog Information

Human gene(s) in FlyBase

Other mammalian ortholog(s) used

D. melanogaster Gene Information (4)

Dmel\Delta

Gene Snapshot

Delta (Delta) encodes a single pass transmembrane EGF family protein and one of two ligands of the Notch signaling pathway. It regulates cell fate decisions and cell proliferation. Post transcriptional modification (such as by fucosylation, ubiquitination and proteolysis) of the product of Delta is key for its functions. [Date last reviewed: 2019-03-07]

Molecular function (GO)

Cellular component (GO)

Gene Groups / Pathways

NTCH-C
DSL

Comments on ortholog(s)

Moderate- to high-scoring ortholog of human DLL1 and DLL4 (1 Drosophila to 2 human); additional less closely related genes in human. Dmel\Dl shares 39% identity and 51-53% similarity with human DLL1 and DLL4.

Orthologs and Alignments from DRSC

DIOPT - DRSC Integrative Ortholog Prediction Tool - Click the link below to search for orthologs in Humans

H. sapiens (Human)

Dmel\Akt

Gene Snapshot

Akt kinase (Akt) encodes the core kinase component of the insulin/insulin-like growth factor pathway. It functions downstream of the product of Pi3K92E and is activated by phosphatidylinositol binding and phosphorylation. It regulates cell growth and survival, stress responses and ageing. [Date last reviewed: 2019-03-07]

Molecular function (GO)

Cellular component (GO)

Gene Groups / Pathways

Comments on ortholog(s)

Moderate- to high-scoring ortholog of human genes AKT2, AKT3, and AKT1 (1 Drosophila to 3 human). Dmel\Akt shares 60-64% identity and 75-77% similarity with these human genes.

Orthologs and Alignments from DRSC

DIOPT - DRSC Integrative Ortholog Prediction Tool - Click the link below to search for orthologs in Humans

H. sapiens (Human)

Dmel\Pi3K92E

Gene Snapshot

Pi3K92E (Pi3K92E) encodes the catalytic subunit of a class I phosphatidylinositol 3-kinase that functions downstream of the product of InR and other receptors during several processes, including cell and tissue growth. [Date last reviewed: 2018-09-13]

Molecular function (GO)

Cellular component (GO)

Gene Groups / Pathways

Comments on ortholog(s)

High-scoring ortholog of human PIK3CD and PIK3CB; moderate-scoring ortholog of PIK3CG and PIK3CA (1 Drosophila to 4 human). Dmel\Pi3K92E shares 31-39% identity and 50-58% similarity with the human genes.

Orthologs and Alignments from DRSC

DIOPT - DRSC Integrative Ortholog Prediction Tool - Click the link below to search for orthologs in Humans

H. sapiens (Human)

Dmel\Pten

Gene Snapshot

Phosphatase and tensin homolog (Pten) encodes a dual lipid and protein phosphatase that primarily counters the effects of the insulin-regulated lipid kinase, encoded by Pi3K92E. It inhibits cell growth, cell proliferation and cellular events controlling cytoskeletal and junctional rearrangements. [Date last reviewed: 2018-10-18]

Molecular function (GO)

Cellular component (GO)

Gene Groups / Pathways

Comments on ortholog(s)

High-scoring ortholog of human PTEN (1 Drosophila to 1 human). Dmel\Pten shares 41% identity and 57% similarity with the human gene.

Orthologs and Alignments from DRSC

DIOPT - DRSC Integrative Ortholog Prediction Tool - Click the link below to search for orthologs in Humans

H. sapiens (Human)

Other Genes Used: Viral, Bacterial, Synthetic (0)

Summary of Physical Interactions (265 groups)

Delta

RNA-RNA

Interacting group

Assay

References

Delta - mir-1

immunohistochemistry, luminiscence technology

(Kwon et al., 2005)

protein-protein

Interacting group

Assay

References

Delta

bead aggregation assay, fluorescence microscopy, inferred by author, atomic force microscopy

(Parks et al., 2006, Ahimou et al., 2004)

Delta - kuz

protease assay, western blot, inferred by author

(Qi et al., 1999)

Delta - mib1

enzymatic study, anti tag coimmunoprecipitation, anti tag western blot

(Daskalaki et al., 2011, Lai et al., 2005)

Delta - N

bead aggregation assay, fluorescence microscopy, inferred by author, affinity technology, anti bait coimmunoprecipitation, cross-linking study, western blot, tag visualisation by alkaline phosphatase activity, tag visualisation, phenotype-based detection assay, enzyme linked immunosorbent assay, atomic force microscopy, pull down

(Pandey et al., 2019, Yamamoto et al., 2012, Acar et al., 2008, Pei and Baker, 2008, Xu et al., 2007, Parks et al., 2006, Ahimou et al., 2004, Bruckner et al., 2000, Wesley, 1999, Rebay et al., 1991, Fehon et al., 1990)

Delta - neur

anti tag coimmunoprecipitation, anti tag western blot, anti bait coimmunoprecipitation, pull down, western blot, enzymatic study

(Liu et al., 2012, Daskalaki et al., 2011, Fontana and Posakony, 2009, Commisso and Boulianne, 2007, Skwarek et al., 2007, Pitsouli and Delidakis, 2005)

Delta - O-fut1

anti tag coimmunoprecipitation, anti tag western blot

(Okajima et al., 2005)

Delta - sca

coimmunoprecipitation, western blot, anti bait coimmunoprecipitation, anti tag coimmunoprecipitation, affinity technology, fluorescence microscopy

(Petruccelli et al., 2018, Mok et al., 2005)

Akt

protein-protein

Interacting group

Assay

References

Akt - Act57B

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Akt - Afg3l2

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Akt - Akap200

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Akt - αCOP

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Akt - Arc42

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Akt - Arf1

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Akt - AsnRS

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Akt - Atpα

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Akt - ATPsynβ

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Akt - ATPsynγ

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Akt - ben

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Akt - βTub60D

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Akt - βTub97EF

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Akt - blw

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Akt - cathD

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Akt - CCT1

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Akt - CCT2

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Akt - CCT4

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Akt - CCT5

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Akt - CCT6

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Akt - CCT7

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Akt - CCT8

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Akt - CG1416

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Akt - CG2614

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Akt - CG5504

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Akt - CG8036

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Akt - CG9281

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Akt - CG15784

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Akt - CG33303

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Akt - Chc

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Akt - clu

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Akt - cpa

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Akt - cpb

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Akt - CtsK1

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Akt - δCOP

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Akt - Desat1

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Akt - Dref

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Akt - Drice

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Akt - Droj2

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Akt - eEF1α1

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Akt - eEF2

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Akt - eIF2α

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Akt - eIF2γ

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Akt - eIF4A

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Akt - eIF4G1

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Akt - FASN1

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Akt - fax

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Akt - fkh

anti tag coimmunoprecipitation, anti tag western blot

(Bolukbasi et al., 2017)

Akt - GCS2β

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Akt - Gfat2

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Akt - Gmppb

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Akt - GstE7

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Akt - Hel25E

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Akt - Hsc70-2

proximity ligation assay, fluorescence microscopy

(Azuma et al., 2021)

Akt - Hsc70-3

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Akt - Hsc70-5

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Akt - Hsp60A

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Akt - hts

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Akt - Ipo9

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Akt - Kap-α3

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Akt - Kcmf1

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Akt - l(1)G0320

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Akt - l(2)09851

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Akt - Ldh

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Akt - lic

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Akt - Lon

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Akt - Mcad

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Akt - MetRS

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Akt - Mkk4

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Akt - mRpL12

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Akt - mts

anti tag coimmunoprecipitation, anti tag western blot

(Gil-Ranedo et al., 2019)

Akt - Nap1

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Akt - Nfs1

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Akt - Nph

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Akt - nst

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Akt - Ogdh1

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Akt - Ost48

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Akt - Pcb

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Akt - pch2

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Akt - PCNA

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Akt - Pdha1

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Akt - pic

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Akt - Pp2A-29B

anti tag coimmunoprecipitation, Identification by mass spectrometry, anti tag western blot

(Vinayagam et al., 2016)

Akt - Psa

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Akt - Pyk

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Akt - Rack1

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Akt - r-l

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Akt - Rop

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Akt - RpL9

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Akt - RpL17

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Akt - RpL23

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Akt - RpL31

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Akt - RpLP1

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Akt - Rpn1

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Akt - Rpn5

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Akt - Rpn9

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Akt - Rpn10

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Akt - Rpn13

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Akt - RpS7

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Akt - RpS9

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Akt - RpS14a

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Akt - RpS15Aa

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Akt - RpS16

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Akt - RpS17

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Akt - Rpt1

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Akt - Rpt2

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Akt - Rpt4

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Akt - Rpt5

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Akt - Rpt6

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Akt - Sams

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Akt - Scox

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Akt - ScsβA

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Akt - ScsβG

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Akt - SERCA

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Akt - Shmt

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Akt - slmb

anti bait coimmunoprecipitation, anti tag western blot, anti tag coimmunoprecipitation, western blot

(Wong et al., 2013)

Akt - smid

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Akt - Sply

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Akt - sqd

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Akt - Srlp

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Akt - SsRβ

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Akt - Tctp

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Akt - TER94

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Akt - trbl

anti bait coimmunoprecipitation, western blot, two hybrid, pull down, autoradiography

(Fischer et al., 2017, Das et al., 2014)

Akt - Trmt2a

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Akt - tud

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Akt - UQCR-C2

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Akt - Vha68-2

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Akt - wal

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Akt - wdb

anti bait coimmunoprecipitation, western blot

(Fischer et al., 2015, Vereshchagina et al., 2008)

Akt - yki

enzymatic study, western blot

(Li et al., 2022)

Akt - yps

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Pi3K92E

protein-protein

Interacting group

Assay

References

Pi3K92E

protein kinase assay, autoradiography

(Weinkove et al., 1997)

Pi3K92E - Act5C

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Pi3K92E - alt

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Pi3K92E - CG15784

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Pi3K92E - CG30122

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Pi3K92E - chico

anti tag coimmunoprecipitation, peptide massfingerprinting, Identification by mass spectrometry

(Breitkopf et al., 2016, Vinayagam et al., 2016)

Pi3K92E - Droj2

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Pi3K92E - eIF3a

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Pi3K92E - eIF3b

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Pi3K92E - eIF3c

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Pi3K92E - eIF3m

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Pi3K92E - eIF4A

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Pi3K92E - Fib

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Pi3K92E - Git

anti tag coimmunoprecipitation, peptide massfingerprinting

(Glatter et al., 2011)

Pi3K92E - Hsc70-3

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Pi3K92E - InR

anti tag coimmunoprecipitation, peptide massfingerprinting, Identification by mass spectrometry

(Vinayagam et al., 2016, Friedman et al., 2011)

Pi3K92E - iPLA2-VIA

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Pi3K92E - Ipo9

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Pi3K92E - Iswi

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Pi3K92E - larp

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Pi3K92E - Mec2

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Pi3K92E - msn

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Pi3K92E - ncd

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Pi3K92E - nonA

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Pi3K92E - Nsun2

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Pi3K92E - Pi3K21B

anti tag coimmunoprecipitation, peptide massfingerprinting, pull down, anti tag western blot, protein kinase assay, autoradiography, Identification by mass spectrometry, western blot, anti bait coimmunoprecipitation

(Breitkopf et al., 2016, Vinayagam et al., 2016, Glatter et al., 2011, Orme et al., 2006, Wittwer et al., 2005, Weinkove et al., 1997)

Pi3K92E - Psi

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Pi3K92E - Pvr

anti tag coimmunoprecipitation, Identification by mass spectrometry, peptide massfingerprinting

(Breitkopf et al., 2016, Vinayagam et al., 2016)

Pi3K92E - Pyk

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Pi3K92E - Ras64B

anti tag coimmunoprecipitation, anti tag western blot, pull down, autoradiography

(Vega-Cuesta et al., 2020)

Pi3K92E - Ras85D

two hybrid, pull down, western blot

(Orme et al., 2006)

Pi3K92E - rin

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Pi3K92E - Rm62

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Pi3K92E - RpL6

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Pi3K92E - RpL7A

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Pi3K92E - RpL8

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Pi3K92E - RpL9

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Pi3K92E - RpL10

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Pi3K92E - RpL13A

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Pi3K92E - RpL14

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Pi3K92E - RpL17

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Pi3K92E - RpL18

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Pi3K92E - RpL19

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Pi3K92E - RpL21

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Pi3K92E - RpL28

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Pi3K92E - RpL35A

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Pi3K92E - RpLP1

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Pi3K92E - RpS6

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Pi3K92E - RpS7

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Pi3K92E - RpS9

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Pi3K92E - RpS11

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Pi3K92E - RpS15

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Pi3K92E - RpS15Aa

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Pi3K92E - RpS17

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Pi3K92E - RpS23

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Pi3K92E - RpS26

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Pi3K92E - Rpt4

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Pi3K92E - RtGEF

anti tag coimmunoprecipitation, peptide massfingerprinting

(Glatter et al., 2011)

Pi3K92E - rump

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Pi3K92E - sta

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Pi3K92E - Syp

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Pi3K92E - vig

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Pi3K92E - vig2

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Pi3K92E - yps

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Pi3K92E - Zn72D

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Pten

protein-protein

Interacting group

Assay

References

Pten - 14-3-3ζ

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Pten - ACC

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Pten - AGO2

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Pten - α-Spec

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Pten - alt

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Pten - ana2

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Pten - AspRS-m

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Pten - Bap111

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Pten - baz

anti tag coimmunoprecipitation, anti tag western blot, two hybrid

(von Stein et al., 2005)

Pten - Brr2

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Pten - Caf1-55

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Pten - CaMKII

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Pten - Cand1

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Pten - CCT1

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Pten - CG2691

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Pten - CG8939

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Pten - CG10333

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Pten - CG13185

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Pten - CG15099

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Pten - CG15784

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Pten - CG30122

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Pten - Cpsf6

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Pten - eIF3a

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Pten - eIF3c

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Pten - eIF3e

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Pten - eIF3m

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Pten - faf

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Pten - Gnf1

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Pten - Gp150

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Pten - gw

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Pten - HDAC1

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Pten - His3:CG31613

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Pten - HnRNP-K

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Pten - Hsc70-3

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Pten - HUWE1

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Pten - kst

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Pten - larp

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Pten - lva

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Pten - MEP-1

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Pten - mRpL12

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Pten - msk

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Pten - ncd

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Pten - ncm

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Pten - Nup98-96

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Pten - Nup107

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Pten - Nup358

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Pten - poe

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Pten - Prp8

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Pten - puf

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Pten - RanGAP

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Pten - Rm62

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Pten - RpS7

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Pten - RpS14a

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Pten - Rpt2

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Pten - ScsβA

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Pten - sta

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Pten - Syp

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Pten - Tep4

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Pten - Uggt

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Pten - wal

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Alleles Reported to Model Human Disease (Disease Ontology) (47 alleles)

Delta

Models Based on Experimental Evidence ( 2 )

Allele

Disease

Evidence

References

Delta^UAS.cDa

model of cancer

CEA with lola^GS88A8, psq^GS88A8

(Even et al., 2019, Vallejo et al., 2011, Bossuyt et al., 2009, Ferres-Marco et al., 2006)

CEA with ato^{ERD.UAS.Tag:MYC}

(Bossuyt et al., 2009)

CEA with bsk^DN.UAS

(Bossuyt et al., 2009)

CEA with ato^RNAi.UAS.J

(Bossuyt et al., 2009)

CEA with Not3^KK102144

(Vicente et al., 2018)

CEA with Not3^GD4068

(Vicente et al., 2018)

CEA with twin^KK108897

(Vicente et al., 2018)

CEA with Pop2^GD12804

(Vicente et al., 2018)

CEA with Rga^GL00386

(Vicente et al., 2018)

CEA with Not1^GD9640

(Vicente et al., 2018)

CEA

(Zhai et al., 2012)

CEA with ct^RNAi.cGa.UAS

(Zhai et al., 2012)

model of hematologic cancer

CEA with ct^RNAi.cGa.UAS, lola^GS88A8, psq^GS88A8

(Zhai et al., 2012)

Delta^DN.UAS

model of dilated cardiomyopathy

CEA

(Kim et al., 2010)

Modifiers Based on Experimental Evidence ( 9 )

Allele

Disease

Interaction

References

Delta^HMS01309

ameliorates brain cancer

modeled by Vps35^E42

(Li et al., 2018)

Delta^9P

ameliorates multiple endocrine neoplasia type 2B

modeled by Ret^MEN2B.GMR

(Read et al., 2005)

ameliorates multiple endocrine neoplasia type 2A

modeled by Ret^MEN2A.GMR

(Read et al., 2005)

Delta^RevF10

ameliorates cancer

modeled by Src64B^UAS.cCa

(Enomoto et al., 2021)

ameliorates amyotrophic lateral sclerosis

modeled by Hsap\TARDBP^UAS.cHa, Ser^RX82

(Zhan et al., 2013)

Delta^S049520

ameliorates multiple endocrine neoplasia type 2B

modeled by Ret^MEN2B.GMR

(Read et al., 2005)

ameliorates multiple endocrine neoplasia type 2A

modeled by Ret^MEN2A.GMR

(Read et al., 2005)

Delta^UAS.cDa

model of cancer

is exacerbated by mys^HMS00043

(Zhai et al., 2012)

is exacerbated by ItgaPS4^KK105806

(Zhai et al., 2012)

is exacerbated by ct^RNAi.cGa.UAS

(Zhai et al., 2012)

is exacerbated by Not3^KG10496

(De Keersmaecker et al., 2013)

is exacerbated by Not3^GD4068

(De Keersmaecker et al., 2013)

is exacerbated by Not3^KK102144

(De Keersmaecker et al., 2013)

Delta^JF02867

ameliorates carcinoma

modeled by Egfr^UAS.cBa, psq^KK111691

(Boukhatmi et al., 2020)

Delta⁰⁵¹⁵¹

exacerbates ataxia telangiectasia

modeled by tefu^RNAi.UAS

(Rimkus et al., 2008)

Delta^KK107312

exacerbates Alzheimer's disease

modeled by Psn^RNAi.UAS.3

(Kang et al., 2024)

Delta^DN.UAS

ameliorates brain cancer

modeled by Vps35^E42

(Li et al., 2018)

DOES NOT ameliorate cancer

modeled by Hipk^UAS.Tag:HA

(Blaquiere et al., 2018)

ameliorates cancer

modeled by Src64B^UAS.cCa

(Enomoto et al., 2021)

Akt

Models Based on Experimental Evidence ( 5 )

Allele

Disease

Evidence

References

Akt¹

model of type 2 diabetes mellitus

CEA with Akt³

(Murillo-Maldonado et al., 2011)

Akt³

model of type 2 diabetes mellitus

CEA with Akt¹

(Murillo-Maldonado et al., 2011)

Akt^EY10012

model of Parkinson's disease

CEA

(Inamdar et al., 2014)

Akt^UAS.Exel

model of alcohol use disorder

CEA

(Eddison et al., 2011)

Akt⁰⁴²²⁶

model of Parkinson's disease

CEA

(Inamdar et al., 2014)

Modifiers Based on Experimental Evidence ( 14 )

Allele

Disease

Interaction

References

Akt^HMS00007

ameliorates cancer

modeled by yki^{S111A.S168A.S250A.UAS.Tag:V5}

(Lee et al., 2021)

exacerbates frontotemporal dementia

modeled by Hsap\CHMP2B^Intron5.UAS

(West et al., 2018)

Akt^GD1361

ameliorates brain glioma

modeled by btl::Egfr^λ.UAS, Pi3K92E^{UAS.Tag:MYC,Tag:PM(hKRAS)}

(Read et al., 2009)

ameliorates acute myeloid leukemia

modeled by Hsap\RUNX1::Hsap\RUNX1T1^UAS.cSa

(Sinenko et al., 2010)

Akt¹

ameliorates carcinoma

modeled by Ras85D^V12.UAS, scrib¹

(Igaki et al., 2006)

Akt³

exacerbates frontotemporal dementia

modeled by Hsap\CHMP2B^Intron5.UAS

(West et al., 2018)

Akt^EY10012

ameliorates alcohol use disorder

modeled by aru^8.128

(Eddison et al., 2011)

Akt^UAS.Exel

ameliorates neurodegenerative disease

modeled by Hsap\HTT^Q93.ex1.UAS

(Liévens et al., 2008)

ameliorates frontotemporal dementia and/or amyotrophic lateral sclerosis 1

modeled by Zzzz\poly-GR^{80.UAS.Tag:FLAG}

(Li et al., 2020)

modeled by Zzzz\poly-PR^{80.UAS.Tag:FLAG}

(Li et al., 2020)

ameliorates cancer

modeled by Ras85D^V12.QUAS.cLa, scrib^{QUAS.HMS01490}

(Bakopoulos et al., 2023)

Akt^UAS.Tag:HA

exacerbates autosomal dominant cerebellar ataxia

modeled by Hsap\ATXN1^82Q.UAS

(Chen et al., 2003)

ameliorates Huntington's disease

modeled by Hsap\HTT^{128Q.1-336.UAS}

(Branco et al., 2008)

exacerbates olivopontocerebellar atrophy

modeled by Hsap\ATXN1^82Q.UAS

(Branco et al., 2008)

Akt^{T342D.S505D.GMR}

ameliorates frontotemporal dementia and/or amyotrophic lateral sclerosis 1

modeled by Zzzz\GGGGCC^36.UAS

(Atilano et al., 2021)

Akt⁰⁴²²⁶

ameliorates autosomal dominant cerebellar ataxia

modeled by Hsap\ATXN1^82Q.UAS

(Chen et al., 2003)

exacerbates Huntington's disease

modeled by Hsap\HTT^{128Q.1-336.UAS}

(Branco et al., 2008)

ameliorates olivopontocerebellar atrophy

modeled by Hsap\ATXN1^82Q.UAS

(Branco et al., 2008)

exacerbates frontotemporal dementia

modeled by Hsap\CHMP2B^Intron5.UAS

(West et al., 2018)

ameliorates cancer

modeled by Csk^Q156Stop, Ras85D^V12.UAS

(Hirabayashi et al., 2013)

Akt^RNAi.UAS.cUa

ameliorates Alzheimer's disease 1

modeled by Hsap\APP^{Aβ42.UAS.cUa}

(Cheng et al., 2021)

Akt^KK100495

ameliorates acute myeloid leukemia

modeled by Hsap\RUNX1::Hsap\RUNX1T1^UAS.cSa

(Sinenko et al., 2010)

ameliorates high grade glioma

modeled by Wdr62^UAS.Tag:HA

(Shohayeb et al., 2020)

Akt^{UAS.Tag:Myr(Unk)}

ameliorates glucose metabolism disease

modeled by Tl^10b.UAS.cUa

(Roth et al., 2018)

Akt^{T342D.UAS.Tag:HA}

ameliorates glucose metabolism disease

modeled by Tl^10b.UAS.cUa

(Roth et al., 2018)

Akt^{ΔPH.UAS.Tag:Myr(Unk)}

exacerbates frontotemporal dementia

modeled by Hsap\CHMP2B^Intron5.UAS

(West et al., 2018)

ameliorates intestinal cancer

modeled by yki^{3SA.lexAop.sfGFP}

(Saavedra et al., 2023)

ameliorates cancer

modeled by yki^{3SA.lexAop.Tag:Myr(Unk),GFP}

(Chen et al., 2022)

ameliorates type 2 diabetes mellitus

modeled by Uch^KO

(Lee et al., 2024)

Pi3K92E

Models Based on Experimental Evidence ( 10 )

Allele

Disease

Evidence

References

Pi3K92E^2H1

model of type 2 diabetes mellitus

CEA

(Murillo-Maldonado et al., 2011)

CEA with Pi3K92E^A

(Murillo-Maldonado et al., 2011)

CEA with Pi3K92E^5W3

(Murillo-Maldonado et al., 2011)

Pi3K92E^5W3

model of type 2 diabetes mellitus

CEA

(Murillo-Maldonado et al., 2011)

CEA with Pi3K92E^2H1

(Murillo-Maldonado et al., 2011)

CEA with Pi3K92E^A

(Murillo-Maldonado et al., 2011)

model of glucose metabolism disease

CEA with Pi3K92E^A

(Álvarez-Rendón and Riesgo-Escovar, 2020)

Pi3K92E^A

model of type 2 diabetes mellitus

CEA with Pi3K92E^2H1

(Murillo-Maldonado et al., 2011)

CEA with Pi3K92E^5W3

(Murillo-Maldonado et al., 2011)

model of glucose metabolism disease

CEA with Pi3K92E^5W3

(Álvarez-Rendón and Riesgo-Escovar, 2020)

Pi3K92E^A2860C.UAS

model of alcohol use disorder

CEA

(Eddison et al., 2011)

Pi3K92E^CAAX.UAS

model of high grade glioma

CEA with btl::Egfr^λ.UAS

(Courant et al., 2022, Ariss et al., 2020)

CEA with Egfr^Act.cUa.UAS

(Pryszlak et al., 2021)

model of heart disease

CEA

(Karekar et al., 2021)

model of glioblastoma

CEA with btl::Egfr^λ.UAS

(Chi et al., 2019)

Pi3K92E^UAS.Exel

model of alcohol use disorder

CEA

(Eddison et al., 2011)

Pi3K92E^{UAS.Tag:MYC,Tag:PM(hKRAS)}

model of brain glioma

CEA with btl::Egfr^λ.UAS

(Read et al., 2013, Read et al., 2009)

model of high grade glioma

CEA with btl::Egfr^λ.UAS

(Jarabo et al., 2022, Losada-Pérez et al., 2022, Conte et al., 2021, Jarabo et al., 2021, Maravat et al., 2021, Vigneswaran et al., 2021, Portela et al., 2020, Chen et al., 2019, Portela et al., 2019, Portela et al., 2019, Agnihotri et al., 2016)

CEA with Hsap\EGFR^Δ.UAS

(Terrasi et al., 2019)

model of glioblastoma

CEA with btl::Egfr^λ.UAS

(Park et al., 2017)

model of cancer

CEA

(Wang et al., 2023, Ganguly et al., 2021)

CEA with Hsap\RAF1^gof.UAS

(Wang et al., 2023)

model of neurodegenerative disease

CEA with btl::Egfr^λ.UAS

(Jarabo et al., 2022)

CEA with cry^UAS.cEa

(Jarabo et al., 2022)

Pi3K92E^GD11228

model of glucose metabolism disease

CEA

(Mascolo et al., 2022)

Pi3K92E^KK102291

model of glucose metabolism disease

CEA

(Mascolo et al., 2022)

Pi3K92E^UAS.cUa

model of high grade glioma

CEA

(Witte et al., 2009)

Modifiers Based on Experimental Evidence ( 8 )

Allele

Disease

Interaction

References

Pi3K92E^CAAX.UAS

exacerbates high grade glioma

modeled by aPKC^{UAS.Tag:CAAX(Unk)}

(Paglia et al., 2017)

ameliorates Huntington's disease

modeled by Hsap\HTT^{Q138.UAS.mRFP1}

(Hansen et al., 2019)

ameliorates type 2 diabetes mellitus

modeled by Uch^KO

(Lee et al., 2024)

Pi3K92E^{D954A.UAS.Tag:MYC}

ameliorates cancer

modeled by dlg1^GD4689, Ras85D^V12.UAS

(Katheder et al., 2017)

exacerbates Parkinson's disease

modeled by DJ-1α^RNAi.UAS

(Yang et al., 2005)

exacerbates Huntington's disease

modeled by Hsap\HTT^{Q138.UAS.mRFP1}

(Hansen et al., 2019)

ameliorates high grade glioma

modeled by Wdr62^UAS.Tag:HA

(Shohayeb et al., 2020)

ameliorates fragile X syndrome

modeled by Fmr1³

(Monyak et al., 2017)

Pi3K92E^UAS.Tag:MYC

ameliorates Parkinson's disease

modeled by DJ-1α^RNAi.UAS

(Yang et al., 2005)

exacerbates autosomal dominant cerebellar ataxia

modeled by Hsap\ATXN1^82Q.UAS

(Chen et al., 2003)

ameliorates Huntington's disease

modeled by Hsap\HTT^{128Q.1-336.UAS}

(Branco et al., 2008)

exacerbates olivopontocerebellar atrophy

modeled by Hsap\ATXN1^82Q.UAS

(Branco et al., 2008)

exacerbates Alzheimer's disease 1

modeled by Hsap\APP^{Aβ42.UAS.cUa}

(Cheng et al., 2021)

Pi3K92E^{UAS.Tag:MYC,Tag:PM(hKRAS)}

ameliorates cancer

modeled by Myc^αTub84B.PBb

(Levayer et al., 2015)

ameliorates obesity

modeled by Itpr^ka1091, Itpr^ug3

(Subramanian et al., 2013)

model of cancer

is exacerbated by Hsap\PTEN^4A.UAS

(Ganguly et al., 2021)

is ameliorated by Hsap\PTEN^C124R.UAS

(Ganguly et al., 2021)

is ameliorated by Hsap\PTEN^D107V.UAS

(Ganguly et al., 2021)

is ameliorated by Hsap\PTEN^F241S.UAS

(Ganguly et al., 2021)

is ameliorated by Hsap\PTEN^G129E.UAS

(Ganguly et al., 2021)

is ameliorated by Hsap\PTEN^H93R.UAS

(Ganguly et al., 2021)

is ameliorated by Hsap\PTEN^M35V.UAS

(Ganguly et al., 2021)

is ameliorated by Hsap\PTEN^P246L.UAS

(Ganguly et al., 2021)

is ameliorated by Hsap\PTEN^R130Q.UAS

(Ganguly et al., 2021)

is ameliorated by Hsap\PTEN^T131I.UAS

(Ganguly et al., 2021)

is ameliorated by Hsap\PTEN^T167N.UAS

(Ganguly et al., 2021)

is ameliorated by Hsap\PTEN^UAS.cPa

(Ganguly et al., 2021)

is ameliorated by Hsap\PTEN^Y138L.UAS

(Ganguly et al., 2021)

is ameliorated by Hsap\PTEN^Y27C.UAS

(Ganguly et al., 2021)

Pi3K92E^GD11228

ameliorates cancer

modeled by Ras85D^V12.UAS, dlg1^GD4689

(Willecke et al., 2011)

ameliorates high grade glioma

modeled by Pi3K92E^{UAS.Tag:MYC,Tag:PM(hKRAS)}, btl::Egfr^λ.UAS

(Losada-Pérez et al., 2022)

Pi3K92E^KK102291

exacerbates cancer

modeled by Delta^UAS.cDa, ct^RNAi.cGa.UAS

(Zhai et al., 2012)

Pi3K92E^HMC05152

ameliorates prostate cancer

modeled by Ras85D^V12.UAS

(Rambur et al., 2020)

Pi3K92E^UAS.cUa

exacerbates high grade glioma

modeled by btl::Egfr^λ.UAS

(Witte et al., 2009)

model of high grade glioma

is exacerbated by btl::Egfr^λ.UAS

(Witte et al., 2009)

Pten

Models Based on Experimental Evidence ( 9 )

Allele

Disease

Evidence

References

Pten^HMS00044

model of cancer

CEA with Ras85D^G13Q.UAS, Rbf^HMS03004

(Parkhitko et al., 2021)

Pten¹¹⁷

model of cancer

CEA

(Han et al., 2021)

CEA with Pten^2L100

(Ganguly et al., 2021)

Pten^2L100

model of cancer

CEA with Pten¹¹⁷

(Ganguly et al., 2021)

Pten^{RNAi.Exel.UAS}

model of high grade glioma

CEA with Ras85D^V12.cUa.UAS

(Gangwani et al., 2020)

Pten^GD13500

model of colorectal cancer

CEA with p53^VDRC.cUa, Ras85D^V12.cUa.UAS, Apc^VDRC.cUa, Med^VDRC.cUa

(Bangi et al., 2016)

CEA with p53^VDRC.cUa, Ras85D^V12.cUa.UAS, Apc^VDRC.cUa

(Bangi et al., 2016)

CEA with Ras85D^V12.cUa.UAS, Apc^VDRC.cUa, Med^VDRC.cUa

(Bangi et al., 2016)

Pten^KK109278

model of cancer

CEA

(Parniewska and Stocker, 2020)

Pten^NIG.5671R

model of disease of metabolism

CEA

(Devilliers et al., 2021)

Pten^RNAi.UAS.cUa

model of intestinal cancer

CEA with Apc^GD1333, Ras85D^V12.cUa.UAS, p53^GD4483

(Quintero and Bangi, 2023)

Pten^sgRNA.UAS

model of colorectal cancer

CEA with Ras85D^V12.cUa.UAS

(Zipper et al., 2022)

Modifiers Based on Experimental Evidence ( 3 )

Allele

Disease

Interaction

References

Pten^UAS.cUa

ameliorates brain glioma

modeled by btl::Egfr^λ.UAS, Pi3K92E^{UAS.Tag:MYC,Tag:PM(hKRAS)}

(Read et al., 2009)

exacerbates Alzheimer's disease 1

modeled by Hsap\APP^{Aβ42.UAS.cBa}

(Hwang et al., 2019)

ameliorates Huntington's disease

modeled by Hsap\HTT^Q128.UAS

(Martin et al., 2021)

Pten^UAS.cGb

ameliorates Alzheimer's disease 1

modeled by Hsap\APP^{Aβ42.UAS.Tag:SS(rPENK)}

(Chiang et al., 2010)

Pten^KK109278

ameliorates chromosome 16p11.2 deletion syndrome, 593-kb

modeled by Pp4-19C^GD9561

(Iyer et al., 2018)

model of cancer

is ameliorated by CG10565^KK102428

(Parniewska and Stocker, 2020)

is exacerbated by B52^KK108253

(Parniewska and Stocker, 2020)

is ameliorated by Girdin^KK101725

(Parniewska and Stocker, 2020)

is ameliorated by Mo25^GD11213

(Parniewska and Stocker, 2020)

is ameliorated by Osbp^KK107784

(Parniewska and Stocker, 2020)

is ameliorated by S6k^KK107986

(Parniewska and Stocker, 2020)

is ameliorated by SF2^GD12097

(Parniewska and Stocker, 2020)

is ameliorated by SF2^HM05199

(Parniewska and Stocker, 2020)

is ameliorated by SF2^HMS00358

(Parniewska and Stocker, 2020)

is ameliorated by SRPK^KK100641

(Parniewska and Stocker, 2020)

is ameliorated by chico^KK107581

(Parniewska and Stocker, 2020)

is exacerbated by Srp54^GD10663

(Parniewska and Stocker, 2020)

is ameliorated by Vha100-1^KK105748

(Parniewska and Stocker, 2020)

is ameliorated by brp^KK108677

(Parniewska and Stocker, 2020)

is ameliorated by mib1^GD11786

(Parniewska and Stocker, 2020)

is ameliorated by Slik^GD11338

(Parniewska and Stocker, 2020)

is ameliorated by cic^KK112641

(Parniewska and Stocker, 2020)

is ameliorated by mask^KK100529

(Parniewska and Stocker, 2020)

is ameliorated by tra2^KK108747

(Parniewska and Stocker, 2020)

is ameliorated by ttv^GD1993

(Parniewska and Stocker, 2020)

is ameliorated by zfh1^KK109931

(Parniewska and Stocker, 2020)

Alleles Representing Disease-Implicated Variants

Genetic Tools, Stocks and Reagents

Sources of Stocks

Contact lab of origin for a reagent not available from a public stock center.

Bloomington Stock Center Disease Page

Related mammalian, viral, bacterial, or synthetic transgenes

Allele

Transgene

Publicly Available Stocks

Selected Drosophila transgenes

Allele

Transgene

Publicly Available Stocks

Akt^{T342D.UAS.Tag:HA}

P{UAS-Akt.T342D.HA}

Akt^{ΔPH.UAS.Tag:Myr(Unk)}

P{UAS-myr-Akt.DeltaPH}

w^*; P{UAS-myr-Akt.ΔPH}3/TM3, Sb¹

Akt^UAS.Tag:HA

P{UAS-Akt.HA}

Akt^{T342D.S505D.GMR}

P{GMR-Akt.T342D.S505D}

P{GMR-Akt.T342D.S505D}1, y¹ w¹¹¹⁸

Akt^UAS.Exel

P{UAS-Akt.Exel}

y¹ w¹¹¹⁸; P{UAS-Akt.Exel}2

Akt^{UAS.Tag:Myr(Unk)}

P{UAS-myr-Akt.V}

Delta^DN.UAS

P{UAS-Delta.DN}

w¹¹¹⁸; Dr¹ e¹/TM3, P{UAS-Delta.DN}TJ1, Sb¹

Pi3K92E^{UAS.Tag:MYC,Tag:PM(hKRAS)}

P{Dp110-CAAX}

P{Dp110-CAAX}1, y¹ w^*

Pi3K92E^{D954A.UAS.Tag:MYC}

P{Dp110^D954A}

y¹ w^*; P{Dp110^D954A}2

Pi3K92E^UAS.Exel

P{UAS-Pi3K92E.Exel}

y¹ w¹¹¹⁸; P{UAS-Pi3K92E.Exel}2

Pi3K92E^CAAX.UAS

P{UAS-Pi3K92E.CAAX}

P{UAS-Pi3K92E.CAAX}1, y¹ w¹¹¹⁸

Pi3K92E^A2860C.UAS

P{UAS-Pi3K92E.A2860C}

y¹ w¹¹¹⁸; P{UAS-Pi3K92E.A2860C}1

Pten^sgRNA.UAS

P{UAS-Apc,Apc2,p53,Med,Pten,cic.sgRNA}

RNAi constructs available

Allele

Transgene

Publicly Available Stocks

Akt^HMS06047

P{TRiP.HMS06047}

y¹ v¹; P{TRiP.HMS06047}attP40/CyO

Akt^{lexAop.HMS00007}

P{lexAop-HMS00007}

Akt^{RNAi.13xlexAop.cZa}

P{13xlexAop-Akt.RNAi.Z}

Akt^GD1361

P{GD1361}

w¹¹¹⁸; P{GD1361}v2902

y¹ v¹; P{TRiP.HM04007}attP2

Akt^HMS00007

P{TRiP.HMS00007}

y¹ v¹; P{TRiP.HMS00007}attP2

y¹ v¹; P{TRiP.HMJ21422}attP40/CyO

P{UAS-Delta.RNAi.XK8}

w¹¹¹⁸; P{GD2642}v37287

y¹ v¹; P{TRiP.JF02867}attP2

Delta^HMS01309

P{TRiP.HMS01309}

y¹ sc^* v¹ sev²¹; P{TRiP.HMS01309}attP2

y¹ sc^* v¹ sev²¹; P{TRiP.GL00520}attP40

w¹¹¹⁸; P{GD11228}v38985

y¹ v¹; P{TRiP.JF02770}attP2/TM3, Sb¹

Pi3K92E^RNAi.UAS.cUa

P{UAS-Pi3K92E.RNAi.U}

Pi3K92E^GL00311

P{TRiP.GL00311}

y¹ sc^* v¹ sev²¹; P{TRiP.GL00311}attP2/TM3, Sb¹

y¹ sc^* v¹ sev²¹; P{TRiP.HMC05152}attP40

Pten^{lexAop.HMS00044}

P{lexAop-HMS00044}

Pten^{RNAi.Exel.UAS}

P{UAS-Pten.RNAi.Exel}

P{UAS-Pten.RNAi.Exel}1, y¹ w¹¹¹⁸

Pten^GD13500

P{GD13500}

w¹¹¹⁸; P{GD13500}v35731

Pten^JF01859

P{TRiP.JF01859}

y¹ v¹; P{TRiP.JF01859}attP2

Pten^JF01987

P{TRiP.JF01987}

y¹ v¹; P{TRiP.JF01987}attP2/TM3, Sb¹

y¹ v¹; P{TRiP.HMS00044}attP2

Selected Drosophila classical alleles

Allele

Allele class

Mutagen

Publicly Available Stocks

Akt^EY10012

P-element activity

y¹ w^67c23; P{EPgy2}Akt^EY10012/TM3, Sb¹ Ser¹

Akt¹

loss of function allele

Akt³

Akt⁰⁴²²⁶

P-element activity

ry⁵⁰⁶ P{PZ}Akt⁰⁴²²⁶/TM3, ry^RK Sb¹ Ser¹

Delta⁰⁵¹⁵¹

P-element activity

Delta^S049520

Delta2-3 transposase

Delta^ΔExon2

amorphic allele - molecular evidence

CRISPR/Cas9

Delta^BX44

amorphic allele - genetic evidence

X ray

Delta^FE27

amorphic allele - genetic evidence

ethyl methanesulfonate

Delta^9P

amorphic allele - genetic evidence

ethyl methanesulfonate

ru¹ hry¹ Diap1^th-1 st¹ cu¹ sr¹ Delta^9P e^s ca¹/TM3, Sb¹

Delta^BE30

amorphic allele - genetic evidence

ethyl methanesulfonate

Delta^B2

amorphic allele - genetic evidence

ethyl methanesulfonate

Delta^B2 e¹/TM6C, Sb¹

Delta³

loss of function allele

spontaneous

Delta³/In(3R)C, sprd¹ e¹

Delta^RevF10

loss of function allele

Delta^RevF10 e^* Ser^RX82 P{neoFRT}82B/TM6B, Tb¹

Delta^M1

loss of function allele

X ray

Delta^BE32

amorphic allele - genetic evidence

ethyl methanesulfonate

Delta^M

loss of function allele

spontaneous

Delta^X

amorphic allele - genetic evidence

Delta^X/In(3L)P, In(3R)P

Delta^vi3

loss of function allele

ethyl methanesulfonate

Delta^vi1

loss of function allele

ethyl methanesulfonate

Delta^CE21

amorphic allele - genetic evidence

ethyl methanesulfonate

Delta^BX38

amorphic allele - genetic evidence

X ray

Delta^CE23

amorphic allele - genetic evidence

ethyl methanesulfonate

Delta^BX39

amorphic allele - genetic evidence

X ray

Delta^KX1

amorphic allele - genetic evidence

X ray

Delta^M3

loss of function allele

ethyl methanesulfonate

Delta^M2

loss of function allele

spontaneous

Delta^EP3816.A

loss of function allele

Delta2-3 transposase

Pi3K92E^KO

amorphic allele - molecular evidence

CRISPR/Cas9

w^*; Pi3K92E^KO/TM3, Sb¹

Pi3K92E^A

P-element activity

w^*; Df(3R)Pi3K92E^A, H^A Pi3K92E^A, P{HBS}3/TM6B, Tb¹

Pi3K92E^5W3

Pi3K92E^2H1

Pten^KO

amorphic allele - molecular evidence

CRISPR/Cas9

w^*; Pten^KO/CyO

Pten^2L100

Pten^c494

loss of function allele

ethyl methanesulfonate

Pten¹¹⁷

loss of function allele

y¹ w^*; Pten¹¹⁷/CyO

References (6)

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