FB2024_03 , released June 25, 2024
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
AGO2, hpRNA:CR46342
FlyBase ID
FBig0000111028
Interaction Network
Interactions Browser links
AGO2 network
hpRNA:CR46342 network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0206362-35.coIP.PCR
Reference
Zhou et al., 2008
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
AGO2 

Ago2

bait

FLAG tag

hpRNA:CR46342 

esi-2.1

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Zhou et al., 2008)
FBrf0207830-2.coIP.NB
Reference
Flynt et al., 2009
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2R+
Participants
Corresponds to
Reported as
Role
Note
AGO2 

AGO2

bait
hpRNA:CR46342 

hp-CG4068B

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from tagged transfected construct; prey produced from endogenous gene.

(Flynt et al., 2009)

Positive control.

(Flynt et al., 2009)
FBrf0210024-12a.coIP.NB
Reference
Miyoshi et al., 2010
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
AGO2 

AGO2

bait
hpRNA:CR46342 

esi-sl-1

prey
hpRNA:CR46342 
hpRNA:CR46342 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
hpRNA:CR46342 
esi-sl-1
sufficient binding region
UUGACUCCAACAAGUUCGCUCC
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from endogenous gene; prey produced from endogenous gene.

(Miyoshi et al., 2010)
FBrf0222990-11.coIP.NB
Reference
Xiong et al., 2013
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
AGO2 

AGO2

bait

FLAG tag

hpRNA:CR46342 

esi-2.1

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Xiong et al., 2013)

Positive control.

(Xiong et al., 2013)
FBrf0223644-1.coIP.NB
Reference
Abe et al., 2014
Description
physical association
Assay
Collection
Source/Stage
organism / maleadult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
AGO2 

AGO2

bait

FLAG tag

hpRNA:CR46342 

esi-2

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult male flies; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Abe et al., 2014)
FBrf0224072-5.coIP.NB
Reference
Yang et al., 2014
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2R+
Participants
Corresponds to
Reported as
Role
Note
AGO2 

AGO2

bait

FLAG tag

hpRNA:CR46342 

esi-2

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Yang et al., 2014)
FBrf0227265-3a.coIP.NB
Reference
Wen et al., 2015
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
AGO2 

AGO2

bait

FLAG tag

hpRNA:CR46342 

hp-CG4068B

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

hpRNA:CR46342 hpRNA is preferentially associated with AGO2 over AGO1.

(Wen et al., 2015)

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Wen et al., 2015)
FBrf0228536-1.coIP.NB
Reference
Iwasaki et al., 2015
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
AGO2 

AGO2

bait

FLAG tag

hpRNA:CR46342 

esi-2

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Iwasaki et al., 2015)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (8)