FlyBase Recombinant Construct Report: P{VALIUM20-EGFP.RNAi.2}

UAS regulatory sequences drive expression of a hairpin RNA that targets the EGFP fluorescent protein and a number of its derivatives (ECFP, EYFP and YFP-2) but does not target plain GFP. The hairpin was made using the following oligos - forward: ctagcagtCAAGGACGACGGCAACTACAAtagttatattcaagcataTTGTAGTTGCCGTCGTCCTTGgcg, reverse: aattcgcCAAGGACGACGGCAACTACAAtatgcttgaatataactaTTGTAGTTGCCGTCGTCCTTGactg.

(Transgenic RNAi Project, 2011.6.14)

UAS regulatory sequences drive expression of a small hairpin RNA which is directed against EGFP (the hairpin contains an inverted repeat of CAAGGACGACGGCAACTACAA).

(Neumüller et al., 2012)

Sequence Data

Sequence (FB)

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA Sequence

Extent

Segments and Size

Total Size

Left end

Right end

Segments

Num

Orientation

Symbol

Reference

Phenotypic Data

Component Allele

Phenotypic Data

Avic\GFP^RNAi.UAS.2

Phenotypic Class Phenotype Manifest In Detailed Description

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Progenitors and Descendants

progenitor(s)

pVALIUM20

(Transgenic RNAi Project, 2011.6.14)

descendant(s)

Comments

Stocks (1)

Stocks containing insertion(s) of this recombinant construct

Bloomington

41554

y¹ sc^* v¹ sev²¹; P{VALIUM20-EGFP.RNAi.2}attP2

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (7)

Reported As

Symbol Synonym

P{UAS-EGFP.shRNA.2}

(FlyBase, 1992-)

P{VALIUM20-EGFP.RNAi.2}

(FlyBase, 1996-)

P{VALIUM20-EGFP.shRNA.2}

(FlyBase, 1996-)

P{VALIUM20-EGFP}

(Transgenic RNAi Project, 2011.6.14, FlyBase, 1996-)

UAS-EGFP-shRNA

(Neumüller et al., 2012)

UAS-GFP^RNAi

(Robles-Murguia et al., 2020)

UAS-sh-GFP

(Dubruille and Loppin, 2015)

Secondary FlyBase IDs

FBtp0067876
FBtp0071999

References (7)

Report Sections

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