UAS regulatory sequences drive expression of a hairpin RNA that targets the EGFP fluorescent protein and a number of its derivatives (ECFP, EYFP and YFP-2) but does not target plain GFP. The hairpin was made using the following oligos - forward: ctagcagtCAAGGACGACGGCAACTACAAtagttatattcaagcataTTGTAGTTGCCGTCGTCCTTGgcg, reverse: aattcgcCAAGGACGACGGCAACTACAAtatgcttgaatataactaTTGTAGTTGCCGTCGTCCTTGactg.
Adult HipHop rescue males - HipHopT:Avic\GFP;HipHop1/Df(3L)BSC776 - expressing Avic\GFPdsRNA.shRNA.Scer\UAS.2 under the control of Scer\GAL4nos.UTR.T:Hsim\VP16 are sterile with severely atrophied testes.
The dsRNA produced by P{VALIUM20-EGFP} targets the EGFP variant of Avic\GFP and a number of other EGFP derivatives (ECFP, EYFP and YFP-2) but does not target plain GFP.