FlyBase Recombinant Construct Report: PBac{13XLexAop2-IVS-Syn21-shi[ts1]-p10}

General Information

Symbol

PBac{13XLexAop2-IVS-Syn21-shi^ts1-p10}

FlyBase ID

FBtp0115094

Feature type

transgenic_transposable_element

Component Allele(s)

shi^{ts1.IVS.Syn21.p10.13xlexAop}

Also Known As

LexAop-shi^ts

Size

Expression Data

Associated insertion(s)

1 available

Molecular map

Description and Uses

Construct components

Component allele shi^{ts1.IVS.Syn21.p10.13xlexAop}

Transgenic product class

dominant_negative_variant

(Pfeiffer et al., 2012)

missense_variant

(Pfeiffer et al., 2012)

Component

Use(s)

Regulatory region(s)

lexAop

binary expression system - regulatory region

Encoded product / tool

shi

Description

13 copies of the lexAop site are fused upstream of the shi¹ coding region (this encodes a temperature-sensitive dominant negative form of shi). A small intron derived from the Mhc gene followed by a synthetic AT-rich 21bp sequence made by combining the Cavener consensus sequence with elements from the Malacosoma neustria nucleopolyhedrovirus (MnNPV) polyhedrin gene has been inserted between the promoter and coding sequences. The 3' UTR (666bp) from the Autographa californica nucleopolyhedrovirus (AcNPV) p10 gene is present downstream of the coding sequence.

(Pfeiffer et al., 2012)

Sequence Data

Sequence (FB)

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA Sequence

Extent

Segments and Size

Total Size

Left end

Right end

Segments

Num

Orientation

Symbol

Reference

Phenotypic Data

Component Allele

Phenotypic Data

shi^{ts1.IVS.Syn21.p10.13xlexAop}

Phenotypic Class Phenotype Manifest In Detailed Description

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Progenitors and Descendants

progenitor(s)

descendant(s)

Comments

Stocks (0)

Stocks containing insertion(s) of this recombinant construct

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (13)

Reported As

Symbol Synonym

13XLexAop2-IVS-Syn21-Shibire^ts1-p10

(Pfeiffer et al., 2012)

13XLexAop2-IVS-syn21-shibire^ts-p10

(Wohl et al., 2023)

Aop-shi^ts

(Scheunemann et al., 2018)

LexAop-Shi^ts1

(Cho et al., 2018)

LexAop-Shi^ts

(Dag et al., 2019)

LexAop-Shibire^ts1

(Behnke et al., 2021)

LexAop-Shibire^ts

(Lei et al., 2022)

LexAop-shi^ts

(Ueno et al., 2020, Krüttner et al., 2015)

LexAop2-Shi^ts1

(Dolan et al., 2018)

LexAopShi^ts

(Sadaf et al., 2015)

PBac{13XLexAop2-IVS-Syn21-shi^ts1-p10}

(FlyBase, 1992-)

lexAop-shi[ts]

(Lamaze et al., 2018)

pJFRC104

(Pfeiffer et al., 2012)

Secondary FlyBase IDs

References (13)

Report Sections

Open Close