UASt regulatory sequences drive expression of the Hsap\PEAR1 open reading frame. The ORF was obtained from the human ORFeome v8.1 collection ( ORF_ID:100068990 ) and corresponds to Ensembl transcript ENST00000292357.7. The endogenous stop codon is present, thus even though three copies of the Tag:HA tag are present downstream of the ORF, they are not expected to form part of the translated protein.