FB2024_03 , released June 25, 2024
Recombinant Construct: P{UASp-α-Cat.R.ΔM1.HA}
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General Information
Symbol
P{UASp-α-Cat.R.ΔM1.HA}
FlyBase ID
FBtp0141963
Feature type
transgenic_transposable_element
Component Allele(s)
α-CatΔM1.UASp.R.Tag:HA
Size
Expression Data
Associated insertion(s)
1 available
Molecular map
Description and Uses
Construct components
Component allele α-CatΔM1.UASp.R.Tag:HA
Transgenic product class
inframe_deletion
(Sarpal et al., 2019)
Component
Use(s)
Regulatory region(s)
UASp
Encoded product / tool
α-Cat
Tagged with
Tag:HA
Description

UASp regulatory sequences drive expression of α-Cat that lacks part of the M region (amino acid residues 1-292 and 399-917 are present), tagged at the C-terminal end with Tag:HA. The α-Cat sequence has been modified to render it resistant to RNAi by either P{UAS-α-Cat.RNAi.1} (5'-ATGTTAAAA-3' changed to 5'-ATGCTGAAG-3' at the start of the coding region) or P{TRiP.HMS00317} (5'-GCAGCATCGATATTGACTGTT-3' changed to 5'-GCCGCCTCCATCCTGACCGTG-3' in the M2 domain).

(Sarpal et al., 2019)
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (0)
Stocks containing insertion(s) of this recombinant construct
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
P{UASp-α-Cat.R.ΔM1.HA}
(FlyBase, 1992-)
UAS-α-CatR-ΔM1
(Sarpal et al., 2019)
α-CatR-ΔM1
(Sheppard et al., 2023)
Secondary FlyBase IDs
    References (3)