UASt regulatory sequences drive expression of the Hsap\GRK4 open reading frame, tagged at the C-terminal end with three copies of Tag:HA. The ORF (originally amplified from the ccsbBroadEn_06321 clone) has been mutated to carry p.P385A (c.1153C>G), a variant associated with Autism Spectrum disorder (PMID:25363768).