UASt regulatory sequences drive expression of the Hsap\TRIP12 open reading frame. The endogenous stop codon is present, thus even though three copies of the Tag:HA tag are present downstream of the ORF, they are not expected to form part of the translated protein. The ORF (originally amplified from the MHS6278-211690497 Clone ID:40083165 clone) has been mutated to carry p.R1643Q (c.4928G>A), a variant associated with Autism Spectrum disorder (PMID:25363768).