FlyBase Allele Report: Dmel\vn[ddd-3]

General Information

Symbol

Dmel\vn^ddd-3

Species

D. melanogaster

Name

FlyBase ID

FBal0002306

Feature type

allele

Associated gene

Dmel\vn

Associated Insertion(s)

Carried in Construct

Also Known As

vn^RG, vn^ddd3, RG436, vn^dddRG

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

(Schnepp et al., 1996, Simcox et al., 1996, Wurst et al., 1984)

Progenitor genotype

Cytology

Polytene chromosomes normal.

(Simcox et al., 1996)

Description

Nucleotide substitution: C?T.

(Donaldson et al., 2004)

Amino acid replacement: Q336term.

(Donaldson et al., 2004)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

3L:5,842,550..5,842,550 [-]

Nucleotide change:

C5842550T

Reported nucleotide change:

C?T

Amino acid change:

Q337term | vn-PA; Q337term | vn-PC

Reported amino acid change:

Q336term

(Donaldson et al., 2004)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal - all die before end of larval stage | partially

(Wurst et al., 1984)

lethal - all die before end of prepupal stage | recessive

(Simcox et al., 1996)

some die during larval stage | partially

(Wurst et al., 1984)

some die during prepupal stage | recessive

(Simcox et al., 1996)

visible | recessive

(Amin et al., 1999, Baonza and Garcia-Bellido, 1999)

Phenotype Manifest In

adult cuticle & head capsule | dorsal

(Amin et al., 1999)

embryonic/larval brain

(Wurst et al., 1984)

haltere disc

(Wurst et al., 1984)

larval ventral nerve cord | larval stage

(Wurst et al., 1984)

ocellar bristle

(Amin et al., 1999)

ocellus

(Amin et al., 1999)

postocellar bristle

(Amin et al., 1999)

wing

(Simcox et al., 1996)

wing disc

(Wurst et al., 1984)

wing disc (with vn^L6)

(Austin et al., 2014)

wing vein L4

(Baonza and Garcia-Bellido, 1999)

Detailed Description

Statement

Reference

vn^L6/vn^ddd-3 wing discs fail to grow beyond a rudimentary size.

(Austin et al., 2014)

In homozygous mutant clones, the ocelli and ocellar bristles are deleted and the postvertical bristles, which lie near the lateral ocelli are also lost. However, most of the interocellar cuticle is retained, although occasionally limited invasion of frons into the ocellar region sometimes occurs. Occasionally irregularities in the dorsal head cuticle are seen.

(Amin et al., 1999)

vn¹/vn^ddd-3 flies show loss of wing vein L4.

(Baonza and Garcia-Bellido, 1999)

vn^ddd-3/vn^ddd-4 embryos exhibit a wild type phenotype. Leg and eye/antenna vn^ddd-3/vn^ddd-4 mutant discs can be in vivo cultured. Wing and haltere discs cannot.

(Simcox, 1997)

Individuals transheterozygous with a vn deletion exhibit small wings.

(Simcox et al., 1996)

The viability of homozygous larvae is reduced, with only 21% surviving to the third instar stage, and only 6% surviving to form pupae. Third instar homozygous larvae have no wing or haltere discs. Cells that probably represent the precursors of these discs are found in the positions where wing and haltere discs are normally found. There are numerous cavities in the brain and ventral ganglion. Homozygous vn^ddd-3 1st and 2nd leg discs cultured in vivo transdetermine to wing at approximately the same frequency as wild-type leg discs.

(Wurst et al., 1984)

L3 larval/pupal lethal

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

vn^ddd-3/vn¹ has visible phenotype, enhanceable by emc^D

(Baonza and Garcia-Bellido, 1999)

Suppressed by

Statement

Reference

vn^ddd-3/vn¹ has visible phenotype, suppressible by emc¹/emc¹¹

(Baonza and Garcia-Bellido, 1999)

NOT Suppressor of

Statement

Reference

vn^ddd-3 is a non-suppressor of visible phenotype of AdamTS-B^KK108644, Scer\GAL4^Act5C.PU

(Butchar et al., 2012)

Phenotype Manifest In

Enhanced by

Statement

Reference

vn^ddd-3/vn¹ has wing vein phenotype, enhanceable by emc^D

(Baonza and Garcia-Bellido, 1999)

Suppressed by

Statement

Reference

vn^ddd-3/vn¹ has wing vein L4 phenotype, suppressible by emc¹/emc¹¹

(Baonza and Garcia-Bellido, 1999)

vn^ddd-3 has phenotype, suppressible by bs⁰³²⁶⁷

(Roch et al., 1998)

NOT Suppressor of

Statement

Reference

vn^ddd-3 is a non-suppressor of wing vein | ectopic phenotype of AdamTS-B^KK108644, Scer\GAL4^Act5C.PU

(Butchar et al., 2012)

Additional Comments

Genetic Interactions

Statement

Reference

The extra wing vein phenotype caused by expression of CG4096^KK108644 under the control of Scer\GAL4^Act5C.PU is not suppressed if the flies also carry vn^ddd-3.

(Butchar et al., 2012)

The vn¹/vn^ddd-3 loss of wing vein L4 phenotype is suppressed by emc¹/emc¹¹. emc^D enhances the loss of wing veins seen in vn¹/vn^ddd-3 flies.

(Baonza and Garcia-Bellido, 1999)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Shearn.

Comments

Homozygous early third instar larvae can support the in vivo culture of wild-type wing discs.

(Wurst et al., 1984)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (10)

Reported As

Symbol Synonym

RG436

(Amin et al., 1999, Wurst et al., 1984)

ddd³

l(3)RG436

vn³

(Butchar et al., 2012)

vn^RG436

(Jussen et al., 2016)

vn^RG

(Austin et al., 2014, Galindo et al., 2005, Galindo et al., 2002, Wang et al., 2000, Simcox, 1997)

vn^ddd-3

vn^ddd3

(Butchar et al., 2012, Baonza and Garcia-Bellido, 1999, Garcia-Bellido et al., 1994)

vn^dddRG436

(Roch et al., 1998)

vn^dddRG

(Schnepp et al., 1996, Simcox et al., 1996)

Name Synonyms

Secondary FlyBase IDs

References (16)

Report Sections

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