FlyBase Allele Report: Dmel\S6k[07084]

General Information

Symbol

Dmel\S6k⁰⁷⁰⁸⁴

Species

D. melanogaster

Name

FlyBase ID

FBal0004707

Feature type

allele

Associated gene

Dmel\S6k

Associated Insertion(s)

P{PZ}S6k⁰⁷⁰⁸⁴

Carried in Construct

Also Known As

dS6K⁰⁷⁰⁸⁴, fs(3)07084, S6k^P1713

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

Progenitor genotype

Associated Insertion(s)

P{PZ}S6k⁰⁷⁰⁸⁴

Cytology

Description

A P{PZ} insertion in the 5' non-coding region of the S6k open reading frame, 48bp upstream of the initial ATG.

(Montagne et al., 1999)

Allele components

Component

Use(s)

Inserted element

P{PZ}

enhancer trap

Encoded product / tool

lacZ

reporter enzyme

Tagged with

Tag:NLS(P)

nuclear protein localization tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

3L:5,810,448..5,810,448 [-]

(Murillo-Maldonado et al., 2011, Cho et al., 2010, Kim et al., 2010, Wang and Huang, 2009, Chen et al., 2008, McClure and Schubiger, 2008, Buffin et al., 2007, Montagne et al., 1999, Spradling et al., 1999, Harvie et al., 1998, BDGP Project Members, 1994-1999)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of type 2 diabetes mellitus

CEA with S6k^l-1

(Murillo-Maldonado et al., 2011)

model of glucose metabolism disease

CEA with S6k^l-1

(Álvarez-Rendón and Riesgo-Escovar, 2023, Álvarez-Rendón and Riesgo-Escovar, 2020)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates autosomal dominant cerebellar ataxia

modeled by Hsap\ATXN8OS^CTG112.UAS

(Mutsuddi et al., 2004)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neurophysiology | adult stage (with S6k^l-1)

(Murillo-Maldonado et al., 2011)

decreased body size

(Montagne et al., 1999)

decreased body size | adult stage (with S6k^l-1)

(Murillo-Maldonado et al., 2011)

decreased cell size

(Montagne et al., 1999)

female sterile

(Perrimon et al., 1996, BDGP Project Members, 1994-1999)

female sterile | recessive

(Spradling et al., 1999, Berkeley Drosophila Genome Project, 1993.6.1)

lethal | recessive

(Ghosh et al., 2015, Harvie et al., 1998)

lethal - all die before end of larval stage | recessive

(Perrimon et al., 1996)

partially lethal

(Montagne et al., 1999)

partially lethal - majority die | recessive

(Berkeley Drosophila Genome Project, 1993.6.1)

some die during larval stage | recessive

(Perrimon et al., 1996)

viable (with S6k^l-1)

(Murillo-Maldonado et al., 2011)

visible

(Montagne et al., 1999)

visible | adult stage (with S6k^l-1)

(Murillo-Maldonado et al., 2011)

Phenotype Manifest In

adult

(Montagne et al., 1999)

oocyte

(Perrimon et al., 1996)

wing (with S6k^l-1)

(Murillo-Maldonado et al., 2011)

Detailed Description

Statement

Reference

S6k^l-1/S6k⁰⁷⁰⁸⁴ mutant adult flies have a reduced body weight compared to heterozygous controls. Wing area is also reduced compared to controls.

Lipid content is increased in S6k^l-1/S6k⁰⁷⁰⁸⁴ mutant flies compared to controls. Carbohydrate content is similar to controls.

S6k^l-1/S6k⁰⁷⁰⁸⁴ mutant flies exhibit defective electroretinogram (ERG) readings.

Cholinesterase activity is significantly reduced in S6k^l-1/S6k⁰⁷⁰⁸⁴ mutant flies compared to controls.

(Murillo-Maldonado et al., 2011)

25% of the expected number of homozygotes emerge as adults with a 3-day delay and reduced body size. Cell numbers are wild-type, and cell size is intermediate between wild-type and S6k^l-1 homozygotes.

(Montagne et al., 1999)

Germline clones produce abnormal eggs due to abnormal oogenesis: no eggs laid, few vitellogenic oocytes.

(Perrimon et al., 1996)

No eggs laid. Df(3L)ZN47/S6k⁰⁷⁰⁸⁴ are female sterile; lethality probably due to secondary mutation.

(BDGP Project Members, 1994-1999)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

S6k[+]/S6k⁰⁷⁰⁸⁴ is an enhancer of abnormal size phenotype of L^fee

(Wang and Huang, 2009)

Suppressor of

Statement

Reference

S6k[+]/S6k⁰⁷⁰⁸⁴, Scer\GAL4^GMR.PU is a suppressor of visible phenotype of Elp6^UAS.cBa, Scer\GAL4^GMR.PU

(Bolukbasi et al., 2012)

S6k[+]/S6k⁰⁷⁰⁸⁴ is a suppressor | partially of short lived phenotype of wrd^KO1

(Hahn et al., 2010)

S6k[+]/S6k⁰⁷⁰⁸⁴ is a suppressor of partially lethal - majority die phenotype of Atg1^EP3348

(Lee et al., 2007)

S6k[+]/S6k⁰⁷⁰⁸⁴ is a suppressor of abnormal neuroanatomy phenotype of Hsap\ATXN8OS^CTG112.UAS, Scer\GAL4^GMR.PF

(Mutsuddi et al., 2004)

Other

Statement

Reference

Keap1^EY02632, S6k⁰⁷⁰⁸⁴/S6k^l-1 has increased rate of adult locomotory behavior phenotype

(Álvarez-Rendón and Riesgo-Escovar, 2023)

Keap1^EY02632, S6k⁰⁷⁰⁸⁴/S6k^l-1 has chemical resistant | adult stage phenotype

(Álvarez-Rendón and Riesgo-Escovar, 2023)

Keap1^EY02632, S6k⁰⁷⁰⁸⁴/S6k^l-1 has abnormal oxidative stress response | adult stage phenotype

(Álvarez-Rendón and Riesgo-Escovar, 2023)

S6k⁰⁷⁰⁸⁴, kri^F1/kri[+] has viable phenotype

(Ghosh et al., 2015)

S6k⁰⁷⁰⁸⁴, kri^H5/kri[+] has viable phenotype

(Ghosh et al., 2015)

Mmus\Rps6kb1^αTub.PM, S6k⁰⁷⁰⁸⁴ has wild-type phenotype

(Montagne et al., 1999)

Phenotype Manifest In

Suppressed by

Statement

Reference

S6k⁰⁷⁰⁸⁴ has phenotype, suppressible by Mmus\Rps6kb1^αTub.PM

(Montagne et al., 1999)

Enhancer of

Statement

Reference

S6k[+]/S6k⁰⁷⁰⁸⁴ is an enhancer of eye phenotype of L^fee

(Wang and Huang, 2009)

Suppressor of

Statement

Reference

S6k[+]/S6k⁰⁷⁰⁸⁴, Scer\GAL4^GMR.PU is a suppressor of eye phenotype of Elp6^UAS.cBa, Scer\GAL4^GMR.PU

(Bolukbasi et al., 2012)

S6k⁰⁷⁰⁸⁴/S6k^l-1 is a suppressor of eye phenotype of Hsap\MAPT^V337M.UAS, Scer\GAL4^GMR.PF

(Khurana et al., 2006)

NOT Suppressor of

Statement

Reference

S6k[+]/S6k⁰⁷⁰⁸⁴ is a non-suppressor of autophagosome | conditional phenotype of Atg1^EP3348

(Lee et al., 2007)

Additional Comments

Genetic Interactions

Statement

Reference

kri^F1/S6k⁰⁷⁰⁸⁴ mutant flies are viable.

kri^H5/S6k⁰⁷⁰⁸⁴ mutant flies are viable.

(Ghosh et al., 2015)

The rough eye phenotype caused by expression of poly^Scer\UAS.cBa under the control of Scer\GAL4^GMR.PU is suppressed by S6k⁰⁷⁰⁸⁴/+.

(Bolukbasi et al., 2012)

The increased rate of egg laying seen in PRAS40^KO females is rescued by one copy of S6k⁰⁷⁰⁸⁴.

(Pallares-Cartes et al., 2012)

L^fee; S6k⁰⁷⁰⁸⁴/+ flies have a reduced eye size compared to L^fee flies.

(Wang and Huang, 2009)

Defects in autophagosome formation in starved Atg1^EP3348 homozygous larvae are not rescued in a S6k⁰⁷⁰⁸⁴ heterozygous background. However, eclosion rates are increased in these animals in a dose-dependent manner and approximately 50% of Atg1^EP3348, S6k⁰⁷⁰⁸⁴/+ animals eclose.

(Lee et al., 2007)

No difference in body weight between S6k⁰⁷⁰⁸⁴/S6k^l-1 scyl³¹/scyl³¹ double mutants and S6k⁰⁷⁰⁸⁴/S6k^l-1 single mutants is seen.

(Reiling and Hafen, 2004)

Xenogenetic Interactions

Statement

Reference

The rough-eye phenotype observed in animals expressing Hsap\MAPT^{V337M.Scer\UAS} under the control of Scer\GAL4^GMR.PF is suppressed in a S6k⁰⁷⁰⁸⁴/S6k^l-1 background.

(Khurana et al., 2006)

One copy of Mmus\S6K^αTub.PM rescues the S6k⁰⁷⁰⁸⁴ phenotype.

(Montagne et al., 1999)

Complementation and Rescue Data

Fails to complement

S6k^l-1

(Montagne et al., 1999)

Rescued by

S6k⁰⁷⁰⁸⁴ is rescued by S6k^+t14.8

(Buffin et al., 2007)

S6k⁰⁷⁰⁸⁴ is rescued by S6k^αTub.PM

(Montagne et al., 1999)

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

11713

P{PZ}S6k⁰⁷⁰⁸⁴ ry⁵⁰⁶/TM3, ry^RK Sb¹ Ser¹

Notes on Origin

Discoverer

A. Spradling.

Comments

Excision of the P-element rescues the phenotype.

(Montagne et al., 1999)

Complements: tsl⁰⁰⁶¹⁷. Complements: vn^rF264.

(BDGP Project Members, 1994-1999)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (10)

Reported As

Symbol Synonym

S6K⁰⁷⁰⁸⁴

(Bolukbasi et al., 2012, Pallares-Cartes et al., 2012, Hahn et al., 2010, Reiling and Hafen, 2004)

S6k⁰⁷⁰⁸⁴

(Ghosh et al., 2015, McClure and Schubiger, 2008, Khurana et al., 2006)

S6k^P1713

(Álvarez-Rendón and Riesgo-Escovar, 2020)

S6k^p1713

(Álvarez-Rendón and Riesgo-Escovar, 2023)

dS6K⁰⁷⁰⁸⁴

(Lee et al., 2007, Montagne et al., 1999)

dS6K^P1713

(Murillo-Maldonado et al., 2011)

fs(3)07084

(Mutsuddi et al., 2004, Spradling et al., 1999)

fs(3)07084⁰⁷⁰⁸⁴

(Perrimon et al., 1996, Meister and Braun, 1995.10, BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project, 1993.6.1)

l(3)07084⁰⁷⁰⁸⁴

p70s6K⁰⁷⁰⁸⁴

Name Synonyms

Secondary FlyBase IDs

FBal0009592

References (24)

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