FlyBase Allele Report: Dmel\Lam[04643]

(Muñoz-Alarcón et al., 2007, Osouda et al., 2005, Patterson et al., 2004, Guillemin et al., 2001, Spradling et al., 1999, BDGP Project Members, 1994-1999)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

dorsal appendage | germline clone

(Guillemin et al., 2001)

dorsal appendage | germline clone | increased number

(Guillemin et al., 2001)

dorsal tracheal branch primordium

(Guillemin et al., 2001)

egg | germline clone

(Guillemin et al., 2001)

egg chamber | germline clone

(Guillemin et al., 2001)

eye disc & nucleus | ectopic | somatic clone

(Patterson et al., 2004)

ganglionic tracheal branch primordium

(Guillemin et al., 2001)

nucleus & eye photoreceptor cell & eye disc

(Kracklauer et al., 2007)

nurse cell | germline clone

(Guillemin et al., 2001)

oocyte | germline clone

(Guillemin et al., 2001)

presumptive embryonic/larval muscle system

(Muñoz-Alarcón et al., 2007)

presumptive embryonic/larval tracheal system

(Guillemin et al., 2001)

Detailed Description

Statement

Reference

Photoreceptor nuclei fail to migrate apically and are positioned randomly within the apical-basal axis in Lam⁰⁴⁶⁴³ mutant eye discs.

(Kracklauer et al., 2007)

Lam⁰⁴⁶⁴³ homozygous and heterozygous mutants exhibit lower pupariation height, indicative of reduced larval locomotion and negative gravitaxis.

Lam⁰⁴⁶⁴³ mutant adult escapers do not exhibit great changes in muscle bulk, placement, or organisation. Muscle 5 in the larval abdominal body wall is often absent or slightly reduced in size and mis-inserted. In addition, fine structure defects in fibrillar organisation are also found in Lam^G262 larvae that are never observed in age-matched control larvae.

(Muñoz-Alarcón et al., 2007)

nuclear membrane | somatic clone | cell autonomous Abnormal clustering of nucleoporins is observed in the nuclear envelope of Lam⁰⁴⁶⁴³ mosaic imaginal disc clones in a cell-autonomous manner. This clustering occurs in clones that have low levels of LamC protein.

(Osouda et al., 2005)

Lam⁰⁴⁶⁴³ heterozygous third instar larvae containing a homozygous eye disc (generated by mitotic recombination using the "EGUF/hid method") are recovered. In the homozygous eye disc the nuclei of R-cells are spread throughout the apical/basal axis of the disc, instead of being exclusively apical, as in wild type.

(Patterson et al., 2004)

20% of homozygous embryos show misguided and stalled terminal tracheal branches at 25^oC. The cytoplasmic projections of the terminal cells do not extend normally; 9% of terminal cells form multiple cytoplasmic blebs, or thin spindle processes that grow in inappropriate directions or stop short before reaching their targets. 2% of terminal cells show defects in nuclear morphology, such as a spherical nucleus protruding off the main axis of the cell. At 29^oC, only 16% of dorsal terminal branches are normal. Females containing homozygous germline clones show occasional egg chambers with oocyte differentiation defects (containing 2 small and 14 large nuclei) and nuclear morphology defects. Egg production is greatly reduced in these females and 86% of eggs are dorsalised; 55% have expanded dorsal appendages, 18% have a single fused or circumferential dorsal appendage, 9% have multiple dorsal appendages and 4% have no dorsal appendage.

(Guillemin et al., 2001)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhancer of

Statement

Reference

Lam[+]/Lam⁰⁴⁶⁴³ is a non-enhancer of visible | heat sensitive phenotype of peb¹

(Wilk et al., 2004)

NOT Suppressor of

Statement

Reference

Lam[+]/Lam⁰⁴⁶⁴³ is a non-suppressor of visible | heat sensitive phenotype of peb¹

(Wilk et al., 2004)

Other

Statement

Reference

Lam⁰⁴⁶⁴³, mod(mdg4)^ul has lethal | embryonic stage phenotype

(Capelson and Corces, 2005)

Phenotype Manifest In

NOT Enhancer of

Statement

Reference

Lam[+]/Lam⁰⁴⁶⁴³ is a non-enhancer of eye | heat sensitive phenotype of peb¹

(Wilk et al., 2004)

NOT Suppressor of

Statement

Reference

Lam[+]/Lam⁰⁴⁶⁴³ is a non-suppressor of eye | heat sensitive phenotype of peb¹

(Wilk et al., 2004)

Additional Comments

Genetic Interactions

Statement

Reference

Body colour: y² ; Lam⁰⁴⁶⁴³/+ ; mod(mdg4)^ul flies show increased production of black pigment in the abdomen compared to y² ; mod(mdg4)^ul flies.

(Capelson and Corces, 2005)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

Lam⁸³

(Patterson et al., 2004)

Lam^A25

(Patterson et al., 2004)

Lam^Ari3

(Patterson et al., 2004)

Lam^Ari7

(Patterson et al., 2004)

Lam^K2

(Patterson et al., 2004)

Lam^sz18

(Guillemin et al., 2001)

Lam^sz9

(Guillemin et al., 2001)

Lam^a7

(Guillemin et al., 2001)

Lam⁰²⁴⁵⁹

(BDGP Project Members, 1994-1999)

Partially rescued by

Lam⁰⁴⁶⁴³ is partially rescued by Lam^UAS.cGa/Scer\GAL4^bs.Term

(Guillemin et al., 2001)

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

11384

P{PZ}Lam⁰⁴⁶⁴³ cn¹/CyO; ry⁵⁰⁶

Notes on Origin

Discoverer

A. Spradling.

Comments

Complements: Hel25E^k11511.

(Fischer, 2003.2.24)

Based on lethality, the following alleles can form an allelic class, from the most severe to the weakest: Lam^P; Lam^D395; Lam⁰⁴⁶⁴³; Lam^G262.

(Muñoz-Alarcón et al., 2007)

Complements: Hel25E^k11511. Complements: cype⁰³⁷⁷¹. Complements: l(2)k07109a^k07109a.

(BDGP Project Members, 1994-1999)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (9)

Reported As

Symbol Synonym

Lam⁰⁴⁶⁴³

(Muñoz-Alarcón et al., 2007, Christensen and Cook, 2006.7.11)

Lam⁴⁶⁴³

(Cavaliere et al., 2020, Kracklauer et al., 2007)

l(2)04643

(Guillemin et al., 2001, Gildea et al., 2000, Spradling et al., 1999)

l(2)04643⁰⁴⁶⁴³

(Meister and Braun, 1995.10, BDGP Project Members, 1994-1999)

lam⁰⁴⁶⁴³

(Cavaliere et al., 2020, Wilk et al., 2004)

lamB⁰⁴⁶⁴³

(Napoletano et al., 2021)

lamin⁰⁴⁶⁴³

(Rohrbaugh et al., 2013)

lamin⁴⁶⁴³

(Capelson and Corces, 2005)

misg⁴⁶⁴³

(Anonymous, 2001, Guillemin et al., 2001)

Name Synonyms

Secondary FlyBase IDs

References (18)

Report Sections

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