FlyBase Allele Report: Dmel\Dp[49Fk-1]

General Information

Symbol

Dmel\Dp^49Fk-1

Species

D. melanogaster

Name

FlyBase ID

FBal0009081

Feature type

allele

Associated gene

Dmel\Dp

Associated Insertion(s)

Carried in Construct

Also Known As

dDP^vr10

Key Links

Genomic Maps

JBrowse

Allele class

hypomorphic allele - genetic evidence

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

hypomorphic allele - genetic evidence

(Myster et al., 2000)

Mutagen

ethyl methanesulfonate

(Duronio et al., 1998, Lasko and Pardue, 1988)

Progenitor genotype

Cytology

Description

Amino acid replacement: R217H.

(Duronio et al., 1998)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

2R:13,226,235..13,226,235 [+]

Nucleotide change:

G13226235A

Amino acid change:

R217H | Dp-PA; R213H | Dp-PB; R189H | Dp-PC

Comment:

site of nucleic acid difference inferred by FlyBase curator based on reported amino acid change

(Duronio et al., 1998)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates Parkinson's disease

modeled by Lrrk^I1915T.UAS

(Gehrke et al., 2010)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

anterior midgut proper primordium & S phase

(Duronio et al., 1998)

dorsal appendage | germline clone

(Myster et al., 2000)

egg | germline clone

(Myster et al., 2000)

embryonic/first instar larval cuticle | germline clone

(Myster et al., 2000)

nurse cell & actin filament | germ-line clone

(Myster et al., 2000)

nurse cell | ectopic | germline clone

(Myster et al., 2000)

nurse cell | germline clone

(Myster et al., 2000)

Detailed Description

Statement

Reference

One copy of Dp^49Fk-1 is unable to suppress position effect variegation (PEV) at the w locus caused by In(1)w^m4.

One copy of Dp^49Fk-1 is unable to suppress the telomeric position effect (TPE) in stocks carrying a variegating P{hsp26-pt-T}39C-5 insertion at the telomere of the left arm of chromosome two.

(Doheny et al., 2008)

Females carrying homozygous germline clones are sterile. Eggs show a range of different chorion phenotypes. 17% of eggs have two somewhat normal dorsal appendages, 80% have a single fused appendage located at the dorsal midline, and 3% have either misplaced appendages or lack appendages all together. 99% of the eggs do not develop to a stage when a cuticle is produced; they are either never fertilised or do not develop sufficiently that a cuticle is secreted. Of the small number of embryos that do develop a cuticle, several show severe but variable pattern defects, while others appear normal. Occasionally a larva hatches from an egg laid by females carrying homozygous germline clones. Young homozygous egg chambers (in females carrying homozygous germline clones) show an aberrant accumulation of actin in several brightly stained foci, which persist throughout oogenesis. Actin bundles do form in mutant nurse cells in late stage egg chambers, but their density is reduced compared to wild type. Mutant nurse cells fail to degenerate on schedule. Approximately 10% of mutant egg chambers contain approximately double the normal number of nurse cell nuclei.

(Myster et al., 2000)

Semilethal over Df(2R)vg56 and Df(2R)vg33 at 25[o]C. Dp^49Fk-1/Df(2R)vg56 and Dp^49Fk-1/Df(2R)vg33 flies do not eclose at 29[o]C. Homozygous stage 13 to 14 embryos incorporate much less BrdU in a 15 minute pulse labeling than wild-type embryos. S phase is longer than in wild-type embryos in cells of the anterior midgut.

(Duronio et al., 1998)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

Dp^49Fk-1 is an enhancer of visible phenotype of Mmus\Shisa5^UAS.cGa, Scer\GAL4^ey.PH

(Gupta et al., 2008)

Dp^49Fk-1 is an enhancer of abnormal size phenotype of Mmus\Shisa5^UAS.cGa, Scer\GAL4^ey.PH

(Gupta et al., 2008)

Dp^49Fk-1 is an enhancer of visible | recessive phenotype of CycE^JP

(Secombe et al., 1998)

NOT Enhancer of

Statement

Reference

Dp^49Fk-1 is a non-enhancer of visible phenotype of upd1^GMR.PB

(Mukherjee et al., 2006)

Dp^49Fk-1 is a non-enhancer of visible phenotype of Dp^GMR.PD, E2f1^GMR.PD

(Staehling-Hampton et al., 1999)

Suppressor of

Statement

Reference

Dp[+]/Dp^49Fk-1 is a suppressor of abnormal neuroanatomy phenotype of Lrrk^I1915T.UAS, Scer\GAL4^ple.PF

(Gehrke et al., 2010)

Dp[+]/Dp^49Fk-1 is a suppressor of abnormal neuroanatomy | recessive phenotype of mir-let7^Δ.cSa

(Gehrke et al., 2010)

NOT Suppressor of

Statement

Reference

Dp^49Fk-1 is a non-suppressor of visible phenotype of upd1^GMR.PB

(Mukherjee et al., 2006)

Dp^49Fk-1 is a non-suppressor of visible phenotype of Dp^GMR.PD, E2f1^GMR.PD

(Staehling-Hampton et al., 1999)

Phenotype Manifest In

Enhancer of

Statement

Reference

Dp^49Fk-1 is an enhancer of eye phenotype of Scer\GAL4^hs.2sev, mbl^C.UAS

(Vicente-Crespo et al., 2008)

Dp^49Fk-1 is an enhancer of eye phenotype of CycE^JP

(Secombe et al., 1998)

NOT Enhancer of

Statement

Reference

Dp^49Fk-1 is a non-enhancer of eye phenotype of upd1^GMR.PB

(Mukherjee et al., 2006)

Suppressor of

Statement

Reference

Dp[+]/Dp^49Fk-1 is a suppressor of dopaminergic neuron phenotype of Lrrk^I1915T.UAS, Scer\GAL4^ple.PF

(Gehrke et al., 2010)

Dp[+]/Dp^49Fk-1 is a suppressor of dopaminergic neuron phenotype of mir-let7^Δ.cSa

(Gehrke et al., 2010)

NOT Suppressor of

Statement

Reference

Dp^49Fk-1 is a non-suppressor of eye phenotype of upd1^GMR.PB

(Mukherjee et al., 2006)

Additional Comments

Genetic Interactions

Statement

Reference

Heterozygosity for Dp^49Fk-1 suppresses the dopaminergic neuronal phenotypes in animals expressing Lrrk^{I1915T.Scer\UAS} under the control of Scer\GAL4^ple.PF.

Heterozygosity for Dp^49Fk-1 suppresses the dopaminergic neuronal phenotypes of homozygous let-7^Δ.cSa animals.

(Gehrke et al., 2010)

Dominantly enhances the rough eye phenotype of CycE^JP homozygotes.

(Secombe et al., 1998)

Xenogenetic Interactions

Statement

Reference

Dp^49Fk-1 enhances the eye phenotypes seen when Mmus\Shisa5^Scer\UAS.cGa is expressed under the control of Scer\GAL4^dpp.PH.

(Gupta et al., 2008)

Complementation and Rescue Data

Fails to complement

Su(z)2⁵

(Doheny et al., 2008)

Rescued by

Df(2R)BSC272/Dp^49Fk-1 is rescued by Dp^+tgDPwt

(Gehrke et al., 2010)

Dp^49Fk-1 is rescued by Dp^+mMa

(Myster et al., 2000)

Partially rescued by

Df(2R)BSC272/Dp^49Fk-1 is partially rescued by Dp^gDPmut

(Gehrke et al., 2010)

Dp^49Fk-1/Df(2R)vg33 is partially rescued by Dp^hs.PD

(Duronio et al., 1998)

Dp^49Fk-1/Df(2R)vg56 is partially rescued by Dp^hs.PD

(Duronio et al., 1998)

Comments

Dp^+tgDPwt rescues the viability of Dp^49Fk-1/Df(2R)BSC272 mutants.

Although Dp^gDPmut rescues the viability of Dp^49Fk-1/Df(2R)BSC272 mutants, the rescued animals show a loss of dopaminergic neurons and locomotor defects.

(Gehrke et al., 2010)

The two major defects seen in females carrying homozygous Dp^49Fk-1 germline clones (loss of dorsal-ventral polarity in the egg and a failure to correctly form a 16-cell cyst) are significantly rescued by Dp^+mMa.

(Myster et al., 2000)

Dp^49Fk-1/Df(2R)vg56 and Dp^49Fk-1/Df(2R)vg33 flies failure to eclose at 29^oC is partially rescued by Dp^hs.PD.

(Duronio et al., 1998)

Images (0)

Mutant

Wild-type

Stocks (2)

5553

108295

Notes on Origin

Discoverer

Lasko.

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (11)

Reported As

Symbol Synonym

49Fk-1

(Mukherjee et al., 2006)

49Fk¹

(Lasko and Pardue, 1988)

DP^vr10-13

(Heriche et al., 2003)

Dp^49Fk-1

(Thomson et al., 2024, Doheny et al., 2008, Gupta et al., 2008, Vicente-Crespo et al., 2008)

dDP^vr10

(Myster et al., 2000, Duronio et al., 1998, Secombe et al., 1998)

dpR217H

(Gehrke et al., 2010)

l(2)13

l(2)49Fk¹

vr10-13

(Duronio et al., 1998)

vr10¹³

(Wu and Howe, 1995)

vr^10-13

(Staehling-Hampton et al., 1999)

Name Synonyms

Secondary FlyBase IDs

References (13)

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