FlyBase Allele Report: Mmus\Shisa5[UAS.cGa]

General Information

Symbol

Mmus\Shisa5^UAS.cGa

Species

M. musculus

Name

FlyBase ID

FBal0283101

Feature type

allele

Associated gene

Mmus\Shisa5

Associated Insertion(s)

Carried in Construct

P{UAS-Shisa5.G}

Transgenic product class

wild_type

(Gupta et al., 2008)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Gupta et al., 2008)

Mutagen

in vitro construct

(Gupta et al., 2008)

Progenitor genotype

Carried in construct

P{UAS-Shisa5.G}

Cytology

Description

UASt regulatory sequences drive expression of full length Mmus\Shisa5.

(Gupta et al., 2008)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Mmus\Shisa5

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal size, with Scer\GAL4^ey.PH

(Gupta et al., 2008)

increased cell death, with Scer\GAL4^bbg-C96

(Gupta et al., 2008)

increased cell death, with Scer\GAL4^dpp.PH

(Gupta et al., 2008)

increased cell death, with Scer\GAL4^GMR.PS

(Gupta et al., 2008)

increased cell death, with Scer\GAL4^sd-SG29.1

(Gupta et al., 2008)

lethal - all die before end of larval stage, with Scer\GAL4^da.G32

(Gupta et al., 2008)

lethal - all die before end of larval stage, with Scer\GAL4^hs.PB

(Gupta et al., 2008)

lethal - all die before end of larval stage, with Scer\GAL4^Tub.PU

(Gupta et al., 2008)

some die during larval stage, with Scer\GAL4^da.G32

(Gupta et al., 2008)

some die during larval stage, with Scer\GAL4^hs.PB

(Gupta et al., 2008)

some die during larval stage, with Scer\GAL4^Tub.PU

(Gupta et al., 2008)

visible, with Scer\GAL4^bbg-C96

(Gupta et al., 2008)

visible, with Scer\GAL4^dpp.PH

(Gupta et al., 2008)

visible, with Scer\GAL4^ey.PH

(Gupta et al., 2008)

visible, with Scer\GAL4^GMR.PS

(Gupta et al., 2008)

visible, with Scer\GAL4^ptc-559.1

(Gupta et al., 2008)

visible, with Scer\GAL4^sd-SG29.1

(Gupta et al., 2008)

Phenotype Manifest In

eye, with Scer\GAL4^ey.PH

(Gupta et al., 2008)

eye, with Scer\GAL4^GMR.PS

(Gupta et al., 2008)

wing, with Scer\GAL4^bbg-C96

(Gupta et al., 2008)

wing, with Scer\GAL4^dpp.PH

(Gupta et al., 2008)

wing, with Scer\GAL4^GMR.PS

(Gupta et al., 2008)

wing, with Scer\GAL4^ptc-559.1

(Gupta et al., 2008)

wing, with Scer\GAL4^sd-SG29.1

(Gupta et al., 2008)

Detailed Description

Statement

Reference

Expression of Mmus\Shisa5^Scer\UAS.cGa under the control of Scer\GAL4^ey.PH results in a reduction in eye size. Although individual ommatidia look normal, their arrangement is irregular. Expression under the control of Scer\GAL4^GMR.PS results in a glossy eye phenotype with black patches in the eye. Ommatidia are fused and eyes exhibit near-complete loss of inter-ommatidial bristles.

Expression of Mmus\Shisa5^Scer\UAS.cGa in the entire wing pouch of the wing disc under the control of Scer\GAL4^sd-SG29.1 results in complete absence of the wing blade.

Expression of Mmus\Shisa5^Scer\UAS.cGa in the A/P boundary of the developing wing under the control of either Scer\GAL4^ptc-559.1 or Scer\GAL4^dpp.PH causes bifurcation of the adult wing.

Expression of Mmus\Shisa5^Scer\UAS.cGa under the control of Scer\GAL4^bbg-C96 causes a serrated wing margin.

Expression of Mmus\Shisa5^Scer\UAS.cGa under the control of either Scer\GAL4^GMR.PS, Scer\GAL4^bbg-C96, Scer\GAL4^dpp.PH or Scer\GAL4^sd-SG29.1 results in apoptosis that coincides with the expression domain.

(Gupta et al., 2008)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Mmus\Shisa5^UAS.cGa, Scer\GAL4^ey.PH has visible phenotype, enhanceable by CycE^AR95

(Gupta et al., 2008)

Mmus\Shisa5^UAS.cGa, Scer\GAL4^ey.PH has visible phenotype, enhanceable by dap^UAS.cLa, Scer\GAL4^ey.PH

(Gupta et al., 2008)

Mmus\Shisa5^UAS.cGa, Scer\GAL4^ey.PH has abnormal size phenotype, enhanceable by dap^UAS.cLa, Scer\GAL4^ey.PH

(Gupta et al., 2008)

Mmus\Shisa5^UAS.cGa, Scer\GAL4^ey.PH has visible phenotype, enhanceable by Dp^49Fk-1

(Gupta et al., 2008)

Mmus\Shisa5^UAS.cGa, Scer\GAL4^ey.PH has abnormal size phenotype, enhanceable by Dp^49Fk-1

(Gupta et al., 2008)

Mmus\Shisa5^UAS.cGa, Scer\GAL4^ey.PH has visible phenotype, enhanceable by Cdk1^E1-24

(Gupta et al., 2008)

Mmus\Shisa5^UAS.cGa, Scer\GAL4^ey.PH has abnormal size phenotype, enhanceable by Cdk1^E1-24

(Gupta et al., 2008)

Mmus\Shisa5^UAS.cGa, Scer\GAL4^ey.PH has visible phenotype, enhanceable by Cdk1^B47

(Gupta et al., 2008)

Mmus\Shisa5^UAS.cGa, Scer\GAL4^ey.PH has abnormal size phenotype, enhanceable by Cdk1^B47

(Gupta et al., 2008)

Mmus\Shisa5^UAS.cGa, Scer\GAL4^ey.PH has abnormal size phenotype, enhanceable by CycE^AR95

(Gupta et al., 2008)

Mmus\Shisa5^UAS.cGa, Scer\GAL4^ey.PH has visible phenotype, enhanceable by Cdk4³

(Gupta et al., 2008)

Mmus\Shisa5^UAS.cGa, Scer\GAL4^ey.PH has abnormal size phenotype, enhanceable by Cdk4³

(Gupta et al., 2008)

Mmus\Shisa5^UAS.cGa, Scer\GAL4^ey.PH has visible phenotype, enhanceable by Dp^a1

(Gupta et al., 2008)

Mmus\Shisa5^UAS.cGa, Scer\GAL4^ey.PH has abnormal size phenotype, enhanceable by Dp^a1

(Gupta et al., 2008)

Suppressed by

Statement

Reference

Mmus\Shisa5^UAS.cGa, Scer\GAL4^bbg-C96 has increased cell death phenotype, suppressible by BacA\p35^GMR.PH, Scer\GAL4^bbg-C96

(Gupta et al., 2008)

Mmus\Shisa5^UAS.cGa, Scer\GAL4^GMR.PS has increased cell death phenotype, suppressible by BacA\p35^GMR.PH, Scer\GAL4^GMR.PS

(Gupta et al., 2008)

Mmus\Shisa5^UAS.cGa, Scer\GAL4^sd-SG29.1 has increased cell death phenotype, suppressible by BacA\p35^GMR.PH, Scer\GAL4^sd-SG29.1

(Gupta et al., 2008)

Mmus\Shisa5^UAS.cGa, Scer\GAL4^dpp.PH has increased cell death phenotype, suppressible | partially by BacA\p35^GMR.PH, Scer\GAL4^dpp.PH

(Gupta et al., 2008)

Mmus\Shisa5^UAS.cGa, Scer\GAL4^ey.PH has visible phenotype, suppressible | partially by CycE^UAS.cRa, Scer\GAL4^ey.PH

(Gupta et al., 2008)

Mmus\Shisa5^UAS.cGa, Scer\GAL4^ey.PH has abnormal size phenotype, suppressible | partially by CycE^UAS.cRa, Scer\GAL4^ey.PH

(Gupta et al., 2008)

Mmus\Shisa5^UAS.cGa, Scer\GAL4^dpp.PH has visible phenotype, suppressible | partially by CycE^UAS.cRa, Scer\GAL4^dpp.PH

(Gupta et al., 2008)

Mmus\Shisa5^UAS.cGa, Scer\GAL4^dpp.PH has increased cell death phenotype, suppressible | partially by CycE^UAS.cRa, Scer\GAL4^dpp.PH

(Gupta et al., 2008)

Mmus\Shisa5^UAS.cGa, Scer\GAL4^ey.PH has visible phenotype, suppressible by Cdk1^UAS.cWa, Scer\GAL4^ey.PH

(Gupta et al., 2008)

Mmus\Shisa5^UAS.cGa, Scer\GAL4^ey.PH has abnormal size phenotype, suppressible by Cdk1^UAS.cWa, Scer\GAL4^ey.PH

(Gupta et al., 2008)

Mmus\Shisa5^UAS.cGa, Scer\GAL4^ey.PH has visible phenotype, suppressible | partially by stg^UAS.cNa, Scer\GAL4^ey.PH

(Gupta et al., 2008)

Mmus\Shisa5^UAS.cGa, Scer\GAL4^ey.PH has abnormal size phenotype, suppressible | partially by stg^UAS.cNa, Scer\GAL4^ey.PH

(Gupta et al., 2008)

NOT suppressed by

Statement

Reference

Mmus\Shisa5^UAS.cGa, Scer\GAL4^ey.PH has abnormal size phenotype, non-suppressible by Cdk2^UAS.Tag:MYC, Scer\GAL4^ey.PH

(Gupta et al., 2008)

Mmus\Shisa5^UAS.cGa, Scer\GAL4^ey.PH has visible phenotype, non-suppressible by Cdk4^UAS.cMa, Scer\GAL4^ey.PH

(Gupta et al., 2008)

Mmus\Shisa5^UAS.cGa, Scer\GAL4^ey.PH has abnormal size phenotype, non-suppressible by Cdk4^UAS.cMa, Scer\GAL4^ey.PH

(Gupta et al., 2008)

Mmus\Shisa5^UAS.cGa, Scer\GAL4^ey.PH has visible phenotype, non-suppressible by dap⁴

(Gupta et al., 2008)

Mmus\Shisa5^UAS.cGa, Scer\GAL4^ey.PH has abnormal size phenotype, non-suppressible by dap⁴

(Gupta et al., 2008)

Mmus\Shisa5^UAS.cGa, Scer\GAL4^bbg-C96 has visible phenotype, non-suppressible by CycE^UAS.cRa, Scer\GAL4^bbg-C96

(Gupta et al., 2008)

Mmus\Shisa5^UAS.cGa, Scer\GAL4^sd-SG29.1 has increased cell death phenotype, non-suppressible by Scer\GAL4^bbg-C96/CycE^UAS.cRa

(Gupta et al., 2008)

Mmus\Shisa5^UAS.cGa, Scer\GAL4^ey.PH has visible phenotype, non-suppressible by CycD^UAS.cMa, Scer\GAL4^ey.PH

(Gupta et al., 2008)

Mmus\Shisa5^UAS.cGa, Scer\GAL4^ey.PH has abnormal size phenotype, non-suppressible by CycD^UAS.cMa, Scer\GAL4^ey.PH

(Gupta et al., 2008)

Mmus\Shisa5^UAS.cGa, Scer\GAL4^ey.PH has visible phenotype, non-suppressible by Cdk2^UAS.Tag:MYC, Scer\GAL4^ey.PH

(Gupta et al., 2008)

Phenotype Manifest In

Enhanced by

Statement

Reference

Mmus\Shisa5^UAS.cGa, Scer\GAL4^ey.PH has eye phenotype, enhanceable by CycE^AR95

(Gupta et al., 2008)

Mmus\Shisa5^UAS.cGa, Scer\GAL4^ey.PH has eye phenotype, enhanceable by Cdk1^E1-24

(Gupta et al., 2008)

Mmus\Shisa5^UAS.cGa, Scer\GAL4^ey.PH has eye phenotype, enhanceable by Cdk1^B47

(Gupta et al., 2008)

Mmus\Shisa5^UAS.cGa, Scer\GAL4^ey.PH has eye phenotype, enhanceable by Cdk4³

(Gupta et al., 2008)

Mmus\Shisa5^UAS.cGa, Scer\GAL4^ey.PH has eye phenotype, enhanceable by Dp^a1

(Gupta et al., 2008)

Mmus\Shisa5^UAS.cGa, Scer\GAL4^ey.PH has eye phenotype, enhanceable by dap^UAS.cLa, Scer\GAL4^ey.PH

(Gupta et al., 2008)

Suppressed by

Statement

Reference

Mmus\Shisa5^UAS.cGa, Scer\GAL4^bbg-C96 has wing phenotype, suppressible by BacA\p35^GMR.PH, Scer\GAL4^bbg-C96

(Gupta et al., 2008)

Mmus\Shisa5^UAS.cGa, Scer\GAL4^GMR.PS has wing phenotype, suppressible by BacA\p35^GMR.PH, Scer\GAL4^GMR.PS

(Gupta et al., 2008)

Mmus\Shisa5^UAS.cGa, Scer\GAL4^sd-SG29.1 has wing phenotype, suppressible by BacA\p35^GMR.PH, Scer\GAL4^sd-SG29.1

(Gupta et al., 2008)

Mmus\Shisa5^UAS.cGa, Scer\GAL4^dpp.PH has wing phenotype, suppressible | partially by BacA\p35^GMR.PH, Scer\GAL4^dpp.PH

(Gupta et al., 2008)

Mmus\Shisa5^UAS.cGa, Scer\GAL4^ey.PH has eye phenotype, suppressible | partially by CycE^UAS.cRa, Scer\GAL4^ey.PH

(Gupta et al., 2008)

Mmus\Shisa5^UAS.cGa, Scer\GAL4^dpp.PH has wing phenotype, suppressible | partially by CycE^UAS.cRa/Scer\GAL4^ey.PH

(Gupta et al., 2008)

Mmus\Shisa5^UAS.cGa, Scer\GAL4^ey.PH has eye phenotype, suppressible by Cdk1^UAS.cWa, Scer\GAL4^ey.PH

(Gupta et al., 2008)

Mmus\Shisa5^UAS.cGa, Scer\GAL4^ey.PH has eye phenotype, suppressible | partially by stg^UAS.cNa, Scer\GAL4^ey.PH

(Gupta et al., 2008)

NOT suppressed by

Statement

Reference

Mmus\Shisa5^UAS.cGa, Scer\GAL4^bbg-C96 has wing phenotype, non-suppressible by CycE^UAS.cRa, Scer\GAL4^bbg-C96

(Gupta et al., 2008)

Mmus\Shisa5^UAS.cGa, Scer\GAL4^ey.PH has eye phenotype, non-suppressible by CycD^UAS.cMa, Scer\GAL4^ey.PH

(Gupta et al., 2008)

Mmus\Shisa5^UAS.cGa, Scer\GAL4^ey.PH has eye phenotype, non-suppressible by Cdk2^UAS.Tag:MYC, Scer\GAL4^ey.PH

(Gupta et al., 2008)

Mmus\Shisa5^UAS.cGa, Scer\GAL4^ey.PH has eye phenotype, non-suppressible by Cdk4^UAS.cMa, Scer\GAL4^ey.PH

(Gupta et al., 2008)

Mmus\Shisa5^UAS.cGa, Scer\GAL4^ey.PH has eye phenotype, non-suppressible by dap⁴

(Gupta et al., 2008)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Co-expression of BacA\p35^GMR.PH suppresses the apoptosis seen when Mmus\Shisa5^Scer\UAS.cGa is expressed under the control of either Scer\GAL4^GMR.PS, Scer\GAL4^sd-SG29.1 or Scer\GAL4^bbg-C96. Partial suppression is seen when Mmus\Shisa5^Scer\UAS.cGa is expressed under the control of Scer\GAL4^dpp.PH.

Expression of CycE^Scer\UAS.cRa significantly suppresses the eye phenotypes seen when Mmus\Shisa5^Scer\UAS.cGa is expressed under the control of Scer\GAL4^ey.PH.

Expression of CycE^Scer\UAS.cRa significantly suppresses the wing phenotypes seen when Mmus\Shisa5^Scer\UAS.cGa is expressed under the control of Scer\GAL4^dpp.PH. The amount of apoptosis seen in the wing disc is also significantly reduced.

Expression of CycE^Scer\UAS.cRa does not suppress the wing phenotypes seen when Mmus\Shisa5^Scer\UAS.cGa is expressed under the control of Scer\GAL4^bbg-C96. The amount of apoptosis seen in the wing disc is not reduced.

CycE^AR95 enhances the eye phenotypes seen when Mmus\Shisa5^Scer\UAS.cGa is expressed under the control of Scer\GAL4^dpp.PH.

Cdk4³ enhances the eye phenotypes seen when Mmus\Shisa5^Scer\UAS.cGa is expressed under the control of Scer\GAL4^dpp.PH.

Dp^49Fk-1 enhances the eye phenotypes seen when Mmus\Shisa5^Scer\UAS.cGa is expressed under the control of Scer\GAL4^dpp.PH.

Dp^a1 enhances the eye phenotypes seen when Mmus\Shisa5^Scer\UAS.cGa is expressed under the control of Scer\GAL4^dpp.PH.

Expression of CycD^Scer\UAS.cMa does not suppress the eye phenotypes seen when Mmus\Shisa5^Scer\UAS.cGa is expressed under the control of Scer\GAL4^ey.PH.

Expression of cdc2c^{Scer\UAS.T:Hsap\MYC} does not suppress the eye phenotypes seen when Mmus\Shisa5^Scer\UAS.cGa is expressed under the control of Scer\GAL4^ey.PH.

Expression of Cdk4^Scer\UAS.cMa does not suppress the eye phenotypes seen when Mmus\Shisa5^Scer\UAS.cGa is expressed under the control of Scer\GAL4^ey.PH.

Expression of dap^Scer\UAS.cLa greatly enhances the eye phenotypes seen when Mmus\Shisa5^Scer\UAS.cGa is expressed under the control of Scer\GAL4^ey.PH.

dap⁴ does not suppress the eye phenotypes seen when Mmus\Shisa5^Scer\UAS.cGa is expressed under the control of Scer\GAL4^dpp.PH.

Expression of cdc2^Scer\UAS.cWa suppresses the eye phenotypes seen when Mmus\Shisa5^Scer\UAS.cGa is expressed under the control of Scer\GAL4^dpp.PH.

cdc2^E1-24 enhances the eye phenotypes seen when Mmus\Shisa5^Scer\UAS.cGa is expressed under the control of Scer\GAL4^dpp.PH.

cdc2^B47 enhances the eye phenotypes seen when Mmus\Shisa5^Scer\UAS.cGa is expressed under the control of Scer\GAL4^dpp.PH.

Expression of stg^Scer\UAS.cNa partially suppresses the eye phenotypes seen when Mmus\Shisa5^Scer\UAS.cGa is expressed under the control of Scer\GAL4^dpp.PH.

(Gupta et al., 2008)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Mmus\Shisa5^Scer\UAS.cGa

Mmus\Shisa5^UAS.cGa

Name Synonyms

Secondary FlyBase IDs

References (1)

Report Sections

Open Close