FlyBase Allele Report: Dmel\CycD[UAS.cMa]

General Information

Symbol

Dmel\CycD^UAS.cMa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Meyer

FlyBase ID

FBal0119680

Feature type

allele

Associated gene

Dmel\CycD

Associated Insertion(s)

Carried in Construct

P{UAS-CycD.M}

Also Known As

UAS-CycD, UAS-Cyclin D

Key Links

Transgenic product class

wild_type

(Meyer et al., 2000)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Meyer et al., 2000)

Mutagen

in vitro construct

(Meyer et al., 2000)

Progenitor genotype

Carried in construct

P{UAS-CycD.M}

Cytology

Description

UASt regulatory sequences drive expression of CycD.

(Meyer et al., 2000)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

CycD

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

decreased cell size, with Scer\GAL4^da.G32

(Emmerich et al., 2004)

increased body size, with Scer\GAL4^da.G32

(Emmerich et al., 2004)

increased cell number, with Scer\GAL4^da.G32

(Emmerich et al., 2004)

Phenotype Manifest In

wing, with Scer\GAL4^da.G32

(Emmerich et al., 2004)

Detailed Description

Statement

Reference

Flies expressing CycD^Scer\UAS.cMa under the control of Scer\GAL4^da.G32 in a wild-type background weigh on average 111% of the weight of control males. The average area of the wing is 105% that of wild-type, the average cell number in the wing is 113% that of wild-type and the average cell size in the wing is slightly reduced compared to wild type.

(Emmerich et al., 2004)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Cdk4^UAS.cMa, CycD^UAS.cMa, Scer\GAL4^Act5C.PP has increased occurrence of cell division | somatic clone | larval stage phenotype, suppressible by Rbf^280.UAS, Scer\GAL4^Act5C.PP

(Xin et al., 2002)

Suppressor of

Statement

Reference

CycD^UAS.cMa/Cdk4^UAS.cMa, Scer\GAL4^hs.2sev is a suppressor | partially of visible phenotype of Scer\GAL4^hs.2sev, ex^UAS.cBa

(Hipfner et al., 2002)

NOT Suppressor of

Statement

Reference

CycD^UAS.cMa, Scer\GAL4^ey.PH is a non-suppressor of visible phenotype of Mmus\Shisa5^UAS.cGa, Scer\GAL4^ey.PH

(Gupta et al., 2008)

CycD^UAS.cMa, Scer\GAL4^ey.PH is a non-suppressor of abnormal size phenotype of Mmus\Shisa5^UAS.cGa, Scer\GAL4^ey.PH

(Gupta et al., 2008)

Other

Statement

Reference

CycD^UAS.cMa, Hsap\MAPT^R406W.UAS, Scer\GAL4^elav.PLu has partially lethal - majority die phenotype

(Khurana et al., 2006)

Cdk4^UAS.cMa, CycD^UAS.cMa, Scer\GAL4^Aug21 has abnormal cell growth phenotype

(Colombani et al., 2005)

Cdk4^UAS.cMa, CycD^UAS.cMa, Scer\GAL4^Act5C.PP has increased occurrence of cell division | somatic clone | larval stage phenotype

(Xin et al., 2002)

Phenotype Manifest In

Suppressed by

Statement

Reference

Cdk4^UAS.cMa, CycD^UAS.cMa, Scer\GAL4^Act5C.PP has S phase | ectopic | somatic clone phenotype, suppressible by Rbf^280.UAS, Scer\GAL4^Act5C.PP

(Xin et al., 2002)

Cdk4^UAS.cMa, CycD^UAS.cMa, Scer\GAL4^Act5C.PP has eye disc posterior to the morphogenetic furrow | somatic clone | larval stage phenotype, suppressible by Rbf^280.UAS, Scer\GAL4^Act5C.PP

(Xin et al., 2002)

Enhancer of

Statement

Reference

CycD^UAS.cMa, Scer\GAL4^GMR.PF is an enhancer of eye phenotype of Hsap\MAPT^E14.UAS, Scer\GAL4^GMR.PF

(Khurana et al., 2006)

CycD^UAS.cMa, Scer\GAL4^GMR.PF is an enhancer of eye phenotype of Hsap\MAPT^V337M.UAS, Scer\GAL4^GMR.PF

(Khurana et al., 2006)

CycD^UAS.cMa/Scer\GAL4^GMR.PF is an enhancer of eye | conditional phenotype of Hsap\MAPT^UAS.cWa, Scer\GAL4^elav.PLu

(Khurana et al., 2006)

Suppressor of

Statement

Reference

CycD^UAS.cMa/Cdk4^UAS.cMa, Scer\GAL4^hs.2sev is a suppressor | partially of eye phenotype of Scer\GAL4^hs.2sev, ex^UAS.cBa

(Hipfner et al., 2002)

NOT Suppressor of

Statement

Reference

CycD^UAS.cMa, Scer\GAL4^ey.PH is a non-suppressor of eye phenotype of Mmus\Shisa5^UAS.cGa, Scer\GAL4^ey.PH

(Gupta et al., 2008)

Other

Statement

Reference

Cdk4^UAS.cMa, CycD^UAS.cMa, Scer\GAL4^Aug21 has ring canal phenotype

(Colombani et al., 2005)

Cdk4^UAS.cMa, CycD^UAS.cMa, Scer\GAL4^en-e16E has wing blade dorsal compartment phenotype

(Hipfner et al., 2002)

Cdk4^UAS.cMa, CycD^UAS.cMa, Scer\GAL4^en-e16E has wing blade posterior compartment phenotype

(Hipfner et al., 2002)

Cdk4^UAS.cMa, CycD^UAS.cMa, Scer\GAL4^Act5C.PP has eye disc | somatic clone phenotype

(Xin et al., 2002)

Cdk4^UAS.cMa, CycD^UAS.cMa, Scer\GAL4^Act5C.PP has S phase | ectopic | somatic clone phenotype

(Xin et al., 2002)

Cdk4^UAS.cMa, CycD^UAS.cMa, Scer\GAL4^Act5C.PP has eye disc posterior to the morphogenetic furrow | somatic clone | larval stage phenotype

(Xin et al., 2002)

Cdk4^UAS.cMa, CycD^UAS.cMa, Scer\GAL4^F4 has salivary gland phenotype

(Datar et al., 2000)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of CycD^Scer\UAS.cMa and Cdk4^Scer\UAS.cMa in the ring gland using Scer\GAL4^P0206 induces autonomous growth.

(Colombani et al., 2005)

Co-expression of both CycD^Scer\UAS.cMa and Cdk4^Scer\UAS.cMa under the control of Scer\GAL4^en-e16E results in overgrowth of the posterior compartment of the wing, significantly increasing the posterior:anterior ratio by 11%. This overgrowth phenotype is unaffected by Df(3L)ban^Δ1/+. Co-expression of both CycD^Scer\UAS.cMa and Cdk4^Scer\UAS.cMa in animals expressing ex^Scer\UAS.cBa under the control of Scer\GAL4^hs.2sev increases the overall size of the eye but has little effect on the roughness and blistering.

(Hipfner et al., 2002)

Co-expression of CycD^Scer\UAS.cMa and Cdk4^Scer\UAS.cMa under the control of Scer\GAL4^Act5C.PP results in ectopic S phase in the posterior part of the developing eye but not in the morphogenetic furrow. Co-expression of Rbf^280.Scer\UAS blocks the ectopic S phase in the eye disc caused by co-expression of CycD^Scer\UAS.cMa and Cdk4^Scer\UAS.cMa under the control of Scer\GAL4^Act5C.PP.

(Xin et al., 2002)

When Cdk4^Scer\UAS.cMa and CycD^Scer\UAS.cDa are driven by either Scer\GAL4^ey.PH no eye phenotype is seen. Co-expression of Cdk4^Scer\UAS.cMa and CycD^Scer\UAS.cDa under the control of Scer\GAL4^F4, results in oversized glands with nuclei containing excessively endoreduplicated polytene chromosomes. Co-expression of CycD^Scer\UAS.cDa with the kinase impaired Cdk4^{D175N.Scer\UAS.T:Hsap\MYC} or cdc2c^{Scer\UAS.T:Hsap\MYC} does not induce this size increase. Co-expression of Rbf^Scer\UAS.cDa and CycD^Scer\UAS.cDa when driven by Scer\GAL4^GMR.PF no eye phenotype is seen. When driven by Scer\GAL4^ey.PH, slightly reduced eyes are seen. This phenotype is slightly enhanced by the addition of Cdk4^{D175N.Scer\UAS.T:Hsap\MYC}. Co-expression of Rbf^Scer\UAS.cDa, Cdk4^Scer\UAS.cMa and CycD^Scer\UAS.cDa, when driven by Scer\GAL4^hs.2sev or Scer\GAL4^GMR.PF leads to a slight eye phenotype. When driven by Scer\GAL4^ey.PH a slightly reduced eye phenotype is seen. When Cdk4^{D175N.Scer\UAS.T:Hsap\MYC} and CycD^Scer\UAS.cDa are driven by Scer\GAL4^ey.PH no eye phenotype is seen. When either Scer\GAL4^hs.2sev or Scer\GAL4^GMR.PF is the driver, no eye overgrowth is seen, though a slight eye phenotype is seen.

(Datar et al., 2000)

Xenogenetic Interactions

Statement

Reference

Expression of CycD^Scer\UAS.cMa does not suppress the eye phenotypes seen when Mmus\Shisa5^Scer\UAS.cGa is expressed under the control of Scer\GAL4^ey.PH.

(Gupta et al., 2008)

The rough-eye phenotype observed in animals expressing Hsap\MAPT^E14.Scer\UAS under the control of Scer\GAL4^GMR.PF is enhanced by co-expression of CycD^Scer\UAS.cMa.

Expression of CycD^Scer\UAS.cMa in adult brains expressing Hsap\MAPT^{R406W.Scer\UAS} under the control of Scer\GAL4^elav.PLu results in semi-lethality.

The rough-eye phenotype observed in animals expressing Hsap\MAPT^{V337M.Scer\UAS} under the control of Scer\GAL4^GMR.PF is enhanced by co-expression of CycD^Scer\UAS.cMa.

(Khurana et al., 2006)

Complementation and Rescue Data

Rescues

CycD^UAS.cMa/Scer\GAL4^da.G32 rescues CycD¹

(Emmerich et al., 2004)

CycD^UAS.cMa/Scer\GAL4^da.G32 rescues CycD²

(Emmerich et al., 2004)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

CycD^Scer\UAS.cMa

CycD^UAS.cMa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Meyer

(Datar et al., 2000, Meyer et al., 2000)

Secondary FlyBase IDs

References (12)

Report Sections

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