FlyBase Allele Report: Hsap\MAPT[E14.UAS]

General Information

Symbol

Hsap\MAPT^E14.UAS

Species

H. sapiens

Name

FlyBase ID

FBal0246848

Feature type

allele

Associated gene

Hsap\MAPT

Associated Insertion(s)

Carried in Construct

P{UAS-Hsap\MAPT.E14}

Also Known As

UAS-Tau^E14, tau^E14, UAS-hTau^E14

Key Links

Transgenic product class

missense_variant

(Khurana et al., 2006)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

missense_variant

(Khurana et al., 2006)

Mutagen

in vitro construct

(Khurana et al., 2006)

Progenitor genotype

Carried in construct

P{UAS-Hsap\MAPT.E14}

Cytology

Description

UASt regulatory sequences drive expression of Hsap\MAPT, carrying mutations in fourteen disease-associated Ser-Thr-Pro sites (Thr 111, Thr 153, Ser 175, Thr 181, Ser 199, Ser 202, Thr 205, Thr 212, Thr 217, Thr 231, Ser 235, Ser 396, Ser 404, and Ser 422) to glutamate.

(Khurana et al., 2006)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Hsap\MAPT

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of tauopathy

CEA

(Zhang et al., 2022, Abtahi et al., 2020, Zhou et al., 2017, Frost et al., 2016, Duboff et al., 2012, Talmat-Amar et al., 2011, Feuillette et al., 2010, Kosmidis et al., 2010, Yeh et al., 2010, Dias-Santagata et al., 2007)

model of Alzheimer's disease

CEA

(Frost et al., 2014)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

model of tauopathy

is exacerbated by Trxr1^Δ1

(Dias-Santagata et al., 2007)

is exacerbated by Sod2ⁿ²⁸³

(Dias-Santagata et al., 2007)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

This allele represents a human variant implicated in disease.

(Khurana et al., 2006)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy, with Scer\GAL4^elav.PU

(Kosmidis et al., 2010)

abnormal neuroanatomy | adult stage, with Scer\GAL4^elav.PU

(Dias-Santagata et al., 2007)

abnormal neuroanatomy | adult stage | progressive, with Scer\GAL4^ple.PF

(Wu et al., 2013)

abnormal neuroanatomy | third instar larval stage, with Scer\GAL4^RapGAP1-OK6

(Talmat-Amar et al., 2011)

increased cell death | adult stage, with Scer\GAL4^elav.PU

(Dias-Santagata et al., 2007)

long lived | heat sensitive, with Scer\GAL4^elav.PU, Scer\GAL80^ts.αTub84B

(Yeh et al., 2010)

partially lethal - majority live, with Scer\GAL4^elav-C155

(Talmat-Amar et al., 2011)

some die during pupal stage, with Scer\GAL4^elav-C155

(Talmat-Amar et al., 2011)

visible, with Scer\GAL4^elav.PU

(Yeh et al., 2010)

visible, with Scer\GAL4^GMR.PF

(Feuillette et al., 2010, Yeh et al., 2010)

visible, with Scer\GAL4^GMR.PU

(Wu et al., 2013)

Phenotype Manifest In

adult brain, with Scer\GAL4^elav.PLu

(Khurana et al., 2006)

adult brain, with Scer\GAL4^elav.PU

(Duboff et al., 2012)

adult brain cell body rind, with Scer\GAL4^elav.PU

(Dias-Santagata et al., 2007)

adult mushroom body, with Scer\GAL4^elav.PU

(Kosmidis et al., 2010)

dopaminergic PPL1 neuron | progressive, with Scer\GAL4^ple.PF

(Wu et al., 2013)

eye, with Scer\GAL4^elav.PU

(Yeh et al., 2010)

eye, with Scer\GAL4^GMR.PF

(Feuillette et al., 2010, Yeh et al., 2010, Yeh et al., 2010, Fulga et al., 2007, Khurana et al., 2006)

eye, with Scer\GAL4^GMR.PU

(Wu et al., 2013)

larval abdominal segmental nerve | third instar larval stage, with Scer\GAL4^RapGAP1-OK6

(Talmat-Amar et al., 2011)

macrochaeta, with Scer\GAL4^Eq

(Yeh et al., 2010)

mitochondrion, with Scer\GAL4^elav.PU

(Duboff et al., 2012)

neuron | adult stage, with Scer\GAL4^elav.PU

(Dias-Santagata et al., 2007)

neuropil | adult stage, with Scer\GAL4^elav.PU

(Dias-Santagata et al., 2007)

sensory organ cell, with Scer\GAL4^Eq

(Yeh et al., 2010)

wing blade | adult stage, with Scer\GAL4^RapGAP1-OK6

(Talmat-Amar et al., 2011)

Detailed Description

Statement

Reference

4 week old flies expressing Hsap\MAPT^E14.Scer\UAS under the control of Scer\GAL4^ple.PF show a significant loss of PPL1 dopaminergic neurons in the brain. No significant loss of these neurons is seen in younger flies.

(Wu et al., 2013)

The mitochondria in the neurons of adult brains expressing Hsap\MAPT^E14.Scer\UAS under the control of Scer\GAL4^elav.PU are markedly elongated. Mitochondria length is on average greater than twice that of controls.

(Duboff et al., 2012)

Overexpression of Hsap\MAPT^E14.UAS under the control of Scer\GAL4^RapGAP1-OK6 in larval segmental nerves effects synaptic vesicle kinetics (there is a significant increase in instant velocity); there is no significant reduction in MT density; no effect on the number of mobile vesicles within axons. Emerging flies have normal wing expansion.

(Talmat-Amar et al., 2011)

Compared to controls, expression of Hsap\MAPT^E14.Scer\UAS under the control of Scer\GAL4^elav.PU results in severe mushroom body (MB) abnormalities. However, although the number of MB neurons is severely reduced, they are not completely eliminated.

(Kosmidis et al., 2010)

Expression of Hsap\MAPT^E14.Scer\UAS in the developing eye under the control of Scer\GAL4^GMR.PF generates a severe rough eye phenotype. The orderly ommatidial architecture is disorganized and photoreceptors are fused with missing mechanosensory bristles.

Expression of Hsap\MAPT^E14.Scer\UAS in the developing external sensory organs under the control of Scer\GAL4^Eq1 generates a moderate degree of bristle loss.

(Yeh et al., 2010)

Expression of Hsap\MAPT^E14.Scer\UAS under the control of Scer\GAL4^GMR.PF results in a severe rough eye phenotype.

Expression of Hsap\MAPT^E14.Scer\UAS under the control of Scer\GAL4^elav.PU results in a rough eye phenotype.

Conditional expression of Hsap\MAPT^E14.Scer\UAS only during the adult stages under the control of Scer\GAL4^elav.PU and Scer\GAL80^ts.αTub84B substantially and significantly elongates the lifespan of flies compared with wild-type.

(Yeh et al., 2010)

Expression of Hsap\MAPT^E14.Scer\UAS under the control of Scer\GAL4^elav.PU results in neurodegeneration of both the cortex and neuropil in 5-day old flies, with these regions exhibiting an increase in neuron apoptosis, as compared to controls.

(Dias-Santagata et al., 2007)

Expression of Hsap\MAPT^E14.Scer\UAS in the retina under the control of Scer\GAL4^GMR.PF results in a rough-eye phenotype.

(Fulga et al., 2007)

Expression of Hsap\MAPT^E14.Scer\UAS under the control of Scer\GAL4^GMR.PF results in a rough-eye phenotype.

Expression of Hsap\MAPT^E14.Scer\UAS in the brain under the control of Scer\GAL4^elav.PLu results in neurodegeneration, and increased cell cycle activation is also observed.

(Khurana et al., 2006)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Hsap\MAPT^E14.UAS, Scer\GAL4^elav.PU has abnormal neuroanatomy | adult stage phenotype, enhanceable by Sod2ⁿ²⁸³/Sod2[+]

(Dias-Santagata et al., 2007)

Hsap\MAPT^E14.UAS, Scer\GAL4^elav.PU has increased cell death | adult stage phenotype, enhanceable by Sod2ⁿ²⁸³/Sod2[+]

(Dias-Santagata et al., 2007)

Hsap\MAPT^E14.UAS, Scer\GAL4^elav.PU has abnormal neuroanatomy | adult stage phenotype, enhanceable by Trxr-1[+]/Trxr1^Δ1

(Dias-Santagata et al., 2007)

Hsap\MAPT^E14.UAS, Scer\GAL4^elav.PU has increased cell death | adult stage phenotype, enhanceable by Trxr-1[+]/Trxr1^Δ1

(Dias-Santagata et al., 2007)

Other

Statement

Reference

Hsap\MAPT^E14.UAS, Scer\GAL4^elav.PU, Trxr-1[+]/Trxr1^Δ1 has short lived phenotype

(Dias-Santagata et al., 2007)

Hsap\MAPT^E14.UAS, Scer\GAL4^elav.PU, Sod2ⁿ²⁸³/Sod2[+] has short lived phenotype

(Dias-Santagata et al., 2007)

Phenotype Manifest In

Enhanced by

Statement

Reference

Hsap\MAPT^E14.UAS, Scer\GAL4^elav.PU has adult brain cell body rind phenotype, enhanceable by Sod2ⁿ²⁸³/Sod2[+]

(Dias-Santagata et al., 2007)

Hsap\MAPT^E14.UAS, Scer\GAL4^elav.PU has neuropil | adult stage phenotype, enhanceable by Sod2ⁿ²⁸³/Sod2[+]

(Dias-Santagata et al., 2007)

Hsap\MAPT^E14.UAS, Scer\GAL4^elav.PU has neuron | adult stage phenotype, enhanceable by Sod2ⁿ²⁸³/Sod2[+]

(Dias-Santagata et al., 2007)

Hsap\MAPT^E14.UAS, Scer\GAL4^elav.PU has adult brain cell body rind phenotype, enhanceable by Trxr-1[+]/Trxr1^Δ1

(Dias-Santagata et al., 2007)

Hsap\MAPT^E14.UAS, Scer\GAL4^elav.PU has neuropil | adult stage phenotype, enhanceable by Trxr-1[+]/Trxr1^Δ1

(Dias-Santagata et al., 2007)

Hsap\MAPT^E14.UAS, Scer\GAL4^elav.PU has neuron | adult stage phenotype, enhanceable by Trxr-1[+]/Trxr1^Δ1

(Dias-Santagata et al., 2007)

Hsap\MAPT^E14.UAS, Scer\GAL4^GMR.PF has eye phenotype, enhanceable by Act5C^UAS.GFP, Scer\GAL4^GMR.PF

(Fulga et al., 2007)

Hsap\MAPT^E14.UAS, Scer\GAL4^GMR.PF has eye phenotype, enhanceable by Act5C^G0010/Act5C[+]

(Fulga et al., 2007)

Hsap\MAPT^E14.UAS, Scer\GAL4^GMR.PF has eye phenotype, enhanceable by CycD^UAS.cMa, Scer\GAL4^GMR.PF

(Khurana et al., 2006)

Hsap\MAPT^E14.UAS, Scer\GAL4^GMR.PF has eye phenotype, enhanceable by CycE^UAS.cUa, Scer\GAL4^GMR.PF

(Khurana et al., 2006)

NOT Enhanced by

Statement

Reference

Hsap\MAPT^E14.UAS, Scer\GAL4^GMR.PF has eye phenotype, non-enhanceable by Hsap\APP^{Aβ42.UAS.Tag:SS(rPENK)}, Scer\GAL4^GMR.PF

(Fulga et al., 2007)

Suppressed by

Statement

Reference

Hsap\MAPT^E14.UAS, Scer\GAL4^GMR.PF has eye phenotype, suppressible by dap^UAS.cdNa/Rbf^UAS.cDa, Scer\GAL4^GMR.PF

(Khurana et al., 2006)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Either Trxr-1^Δ1 heterozygotes or Sod2ⁿ²⁸³ heterozygotes expressing Hsap\MAPT^E14.Scer\UAS under the control of Scer\GAL4^elav.PU are short lived as compared to controls.

(Dias-Santagata et al., 2007)

Expression of Act5C^{Scer\UAS.T:Avic\GFP} in animals expressing Hsap\MAPT^E14.Scer\UAS in the retina under the control of Scer\GAL4^GMR.PF enhances the rough-eye phenotype.

The rough-eye phenotype observed in animals expressing Hsap\MAPT^E14.Scer\UAS in the retina under the control of Scer\GAL4^GMR.PF is enhanced in a Act5C^G0010/+ background.

Expression of Hsap\APP^{Aβ42.Scer\UAS} in flies expressing Hsap\MAPT^E14.Scer\UAS under the control of Scer\GAL4^GMR.PF does not enhance the rough-eye phenotype.

(Fulga et al., 2007)

The rough-eye phenotype observed in animals expressing Hsap\MAPT^E14.Scer\UAS under the control of Scer\GAL4^GMR.PF is suppressed by co-expression of dap^{Scer\UAS.cdNa} and Rbf^Scer\UAS.cDa.

The rough-eye phenotype observed in animals expressing Hsap\MAPT^E14.Scer\UAS under the control of Scer\GAL4^GMR.PF is enhanced by co-expression of CycD^Scer\UAS.cMa.

The rough-eye phenotype observed in animals expressing Hsap\MAPT^E14.Scer\UAS under the control of Scer\GAL4^GMR.PF is enhanced by co-expression of CycE^Scer\UAS.cUa.

(Khurana et al., 2006)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Hsap\MAPT^E14.Scer\UAS

Hsap\MAPT^E14.UAS

tau^E14

(Frost et al., 2016)

Name Synonyms

Secondary FlyBase IDs

References (18)

Report Sections

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