FlyBase Allele Report: Dmel\ex[UAS.cBa]

Ectopic expression of ex^Scer\UAS.cBa under the control of Scer\GAL4^nub.PU results in severely undergrown wings and expression under Scer\GAL4^GMR.PU results in loss of eye tissue and smaller eyes in adult flies.

(Gaspar et al., 2015)

Expression of ex^Scer\UAS.cBa under the control of Scer\GAL4^C587 has no apparent germarium phenotype.

(Li et al., 2015)

Expression of ex^Scer\UAS.cBa under the control of Scer\GAL4^GMR.PF induces a stripe of dying cells posterior to the morphogenetic furrow in third instar eye discs.

(Koontz et al., 2013)

Expression of ex^Scer\UAS.cBa under the control of Scer\GAL4^32B results in a substantial reduction in wing size.

(Angus et al., 2012)

Cells in the wing disc expressing ex^Scer\UAS.cBa under the control of Scer\GAL4^hh-Gal4 grow very poorly.

Expression of ex^Scer\UAS.cBa under the control of Scer\GAL4^hh-Gal4 from the early third larval instar onwards (the temperature sensitive Scer\GAL80^ts.αTub84B allele is used to restrict expression before this stage) does not disrupt the apical F-actin network in the wing disc.

(Fernández et al., 2011)

Expression of ex^UAS.cBa under the control of Scer\GAL4^dpp.PU causes slight undergrowth of the expressing region of the wing disc in the larva. Expression of ex^UAS.cBa under the control of Scer\GAL4^nub.PU causes an extreme reduction in the size of the adult wing.

(Sansores-Garcia et al., 2011)

Expression of ex^Scer\UAS.cBa in the germline, under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16}, does not result in germ-cell over-proliferation, or any alteration of cell morphology or loss of fertility.

Expression of ex^Scer\UAS.cBa in the somatic cells of the testis, under the control of Scer\GAL4^C587, results in non-autonomous over-proliferation of spermatogonial cells.

(Sun et al., 2008)

Expression of ex^Scer\UAS.cBa, under the control of Scer\GAL4^GMR.PF at 25^oC elicits a severe reduction in eye size.

(Bennett and Harvey, 2006)

Larval wing disc clones of cells overexpressing ex^Scer\UAS.cBa under the control of Scer\GAL4^{Scer\FRT.Act5C} are normally composed of only a few cells and are lost over time.

(Cho et al., 2006)

ex^Scer\UAS.cBa; Scer\GAL4^GMR.PF flies have severely reduced and mispatterned eyes. At midpupal stages, the developing retinas in these animals are disorganised with very reduced numbers of inter-ommatidial cells.

Increased levels of cell death are seen in the eye discs of these animals at late third instar.

(Hamaratoglu et al., 2006)

Expression of ex^Scer\UAS.cBa under the control of Scer\GAL4^hs.2sev results in a small, externally roughened eye. Expression of ex^Scer\UAS.cBa under the control of Scer\GAL4^dpp.blk1 results in a reduction of the L3-L4 intervein area. Flies expressing ex^Scer\UAS.cBa under the control of Scer\GAL4^{mir-278-Gal4KI} are lean, measured as the normalised ratio of total body triglycerides to total protein.

(Teleman and Cohen, 2006)

Overexpression of ex^Scer\UAS.cBa during eye development, under the control of Scer\GAL4^GMR.PF severely disrupts eye development and results in small and rough eyes.

(Willecke et al., 2006)

Expression of ex^Scer\UAS.cBa under the control of Scer\GAL4^hs.2sev results in smaller eyes than normal and roughening and blistering of the eye.

(Hipfner et al., 2002)

Expression of ex^Scer\UAS.cBa under the control of Scer\GAL4^ey.PH leads to adult eyes that are often bulged and misshapened, and reduced in the ventral region. Mild eye roughening is seen. Mild patterning defects in the eye are seen, primarily affecting the position of the equator. Expression of ex^Scer\UAS.cBa under the control of Scer\GAL4^32B, leads to external roughening of the eye, reflected ommatidial rotations and a loss of pigment cells are seen. Expression of ex^Scer\UAS.cBa under the control of Scer\GAL4^GMR.PF leads to results in severe defects in eye development - adult eyes are blistered over the entire surface and are reduced in size. Expression of ex^Scer\UAS.cBa under the control of Scer\GAL4^hs.2sev results in severe defects in eye development - adult eyes are blistered and reduced in size. Blistering is visible in anterior regions but morphology is relatively normal in posterior ommatidial rows. The blistered areas show highly disorganised ommatidia. The non-blistered areas exhibit a lack of pigment cells, defects in photoreceptor recruitment and defects in planar polarity. Expression of ex^Scer\UAS.cBa under the control of Scer\GAL4^ey.PH produces defects in the eye disc. Discs are not flat and small relative to the antennal portions of the same disc. However, ommatidial preclusters look fairly well ordered. Expression of ex^Scer\UAS.cBa under the control of Scer\GAL4^32B in eye discs leads to normal alignment of photoreceptor precursor pairs, but misrotated clusters are observed even in more anterior rows. Rotation defects are also prominent in anterior rows if ex^Scer\UAS.cBa is driven by Scer\GAL4^hs.2sev or Scer\GAL4^GMR.PF. In Scer\GAL4^hs.2sev, ex^Scer\UAS.cBa discs one of the R3/R4 precursor pair is occasionally missing. The number of accessory cells which normally surround the photoreceptors appears to be greatly reduced. Organisation of ommatidia is severely disrupted and in many cases ommatidial preclusters have too few cone cell nuclei. Expression of ex^Scer\UAS.cBa under the control of Scer\GAL4^hs.2sev, Scer\GAL4^GMR.PF or Scer\GAL4^ey.PH causes an increase in apoptosis (as indicated by incorporation of Acridine Orange) in the eye disc. Expression of ex^Scer\UAS.cBa under the control of Scer\GAL4^bi-omb-Gal4 results in increased cell death in the wing pouch and parts of the leg disc. producing adult flies with severely reduced wings with patterning defects including loss of vein material and disruption of the margin. Legs appear to be fragile and twisted. Expression of ex^Scer\UAS.cBa under the control of Scer\GAL4^Bx-MS1096 results in small, unpatterned wings and misshapen legs.

(Blaumueller and Mlodzik, 2000)

When expression is driven by Scer\GAL4^32B in an otherwise wild type background the wing area is severely reduced. There is greater size reduction between L2 and L3 than anywhere else. Reduction in size is due to reduced cell number, not size. Some wings show ectopic anterior crossveins, with delta-like wing veins and notched wing margins appearing at lower frequencies.

(Boedigheimer et al., 1997)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Scer\GAL4^hs.2sev, ex^UAS.cBa has visible phenotype, enhanceable by +/Df(3L)mir-ban^Δ1

(Hipfner et al., 2002)

NOT Enhanced by

Statement

Reference

Scer\GAL4^hs.2sev, ex^UAS.cBa has visible phenotype, non-enhanceable by Cdk4³/Cdk4[+]

(Hipfner et al., 2002)

Suppressed by

Statement

Reference

Scer\GAL4^GMR.PU, ex^UAS.cBa has visible | adult stage phenotype, suppressible | partially by Zyx^{UAS.Tag:Myr(Src42A)}/Scer\GAL4^GMR.PU

(Gaspar et al., 2015)

Scer\GAL4^nub.PU, ex^UAS.cBa has visible | adult stage phenotype, suppressible by Scer\GAL4^nub.PU/Zyx^UAS.cGa

(Gaspar et al., 2015)

Scer\GAL4^nub.PU, ex^UAS.cBa has hypoplasia | adult stage phenotype, suppressible by Scer\GAL4^nub.PU/Zyx^UAS.cGa

(Gaspar et al., 2015)

Scer\GAL4^GMR.PF, ex^UAS.cBa has increased cell death | third instar larval stage phenotype, suppressible | partially by sd^JF02514/Scer\GAL4^GMR.PF

(Koontz et al., 2013)

Scer\GAL4^GMR.PF, ex^UAS.cBa has increased cell death | third instar larval stage phenotype, suppressible | partially by Tgi^GD7081/Scer\GAL4^GMR.PF

(Koontz et al., 2013)

Scer\GAL4^FRT.Act5C, ex^UAS.cBa has decreased cell number | somatic clone | larval stage phenotype, suppressible by wts^x1

(Cho et al., 2006)

Scer\GAL4^FRT.Act5C, ex^UAS.cBa has decreased cell number | somatic clone | larval stage phenotype, suppressible by BacA\p35^UAS.cHa, Scer\GAL4^FRT.Act5C

(Cho et al., 2006)

Scer\GAL4^GMR.PF, ex^UAS.cBa has increased cell death | third instar larval stage phenotype, suppressible | partially by hpo^42-47/hpo^42-47

(Hamaratoglu et al., 2006)

Scer\GAL4^GMR.PF, ex^UAS.cBa has increased cell death | third instar larval stage phenotype, suppressible | partially by wts^x1/wts^x1

(Hamaratoglu et al., 2006)

Scer\GAL4^hs.2sev, ex^UAS.cBa has visible phenotype, suppressible | partially by mir-278^UAS.cTa/Scer\GAL4^hs.2sev

(Teleman and Cohen, 2006)

Scer\GAL4^hs.2sev, ex^UAS.cBa has visible phenotype, suppressible | partially by CycD^UAS.cMa/Cdk4^UAS.cMa, Scer\GAL4^hs.2sev

(Hipfner et al., 2002)

Scer\GAL4^hs.2sev, ex^UAS.cBa has visible phenotype, suppressible by mir-ban^EP3622/Scer\GAL4^hs.2sev

(Hipfner et al., 2002)

Scer\GAL4^hs.2sev, ex^UAS.cBa has visible phenotype, suppressible by BacA\p35^UAS.cHa, Scer\GAL4^hs.2sev

(Blaumueller and Mlodzik, 2000)

NOT suppressed by

Statement

Reference

Scer\GAL4^nub.PU, ex^UAS.cBa has visible | adult stage phenotype, non-suppressible by Zyx^{Δ208-231.UAS}/Scer\GAL4^nub.PU

(Gaspar et al., 2015)

Scer\GAL4^nub.PU, ex^UAS.cBa has hypoplasia | adult stage phenotype, non-suppressible by Zyx^{Δ208-231.UAS}/Scer\GAL4^nub.PU

(Gaspar et al., 2015)

Scer\GAL4^hs.2sev, ex^UAS.cBa has visible phenotype, non-suppressible by Cdk4³/Cdk4[+]

(Hipfner et al., 2002)

Suppressor of

Statement

Reference

Scer\GAL4^Act.PU/ex^UAS.cBa is a suppressor of abnormal size | adult stage | somatic clone - Minute background phenotype of Df(3L)H99, mop^sfv3

(Gilbert et al., 2011)

Scer\GAL4^C855a/ex^UAS.cBa is a suppressor of abnormal neuroanatomy phenotype of l(3)mbt^GM76

(Richter et al., 2011)

ex^UAS.cBa/Scer\GAL4^dpp.PU is a suppressor | partially of neoplasia | larval stage phenotype of Scer\GAL4^dpp.PU, dia^{CA.UAS.Tag:HA}

(Sansores-Garcia et al., 2011)

hpo^UAS.Tag:FLAG, ex^UAS.cBa, Scer\GAL4^Act5C.PI, wts^UAS.Tag:MYC is a suppressor of neoplasia | somatic clone | larval stage phenotype of Scer\GAL4^Act5C.PI, yki^{S111A.S168A.S250A.UAS.Tag:V5}

(Oh et al., 2009)

hpo^UAS.Tag:FLAG, ex^UAS.cBa, wts^UAS.Tag:MYC, Scer\GAL4^GMR.PU is a suppressor of neoplasia | larval stage phenotype of Scer\GAL4^GMR.PU, yki^{S111A.S168A.S250A.UAS.Tag:V5}

(Oh et al., 2009)

hpo^UAS.Tag:FLAG, ex^UAS.cBa, Scer\GAL4^GMR.PU is a suppressor | partially of neoplasia | larval stage phenotype of Scer\GAL4^GMR.PU, yki^{S111A.S168A.S250A.UAS.Tag:V5}

(Oh et al., 2009)

ex^UAS.cBa/Scer\GAL4^GMR.PF is a suppressor | partially of abnormal cell growth | somatic clone phenotype of ft⁴²²

(Willecke et al., 2006)

NOT Suppressor of

Statement

Reference

ex^UAS.cBa/Scer\GAL4^GMR.PU is a non-suppressor of neoplasia | larval stage phenotype of Scer\GAL4^GMR.PU, yki^{S111A.S168A.S250A.UAS.Tag:V5}

(Oh et al., 2009)

ex^UAS.cBa, wts^UAS.Tag:MYC, Scer\GAL4^GMR.PU is a non-suppressor of neoplasia | larval stage phenotype of Scer\GAL4^GMR.PU, yki^{S111A.S168A.S250A.UAS.Tag:V5}

(Oh et al., 2009)

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^hs.2sev, ex^UAS.cBa has eye phenotype, enhanceable by +/Df(3L)mir-ban^Δ1

(Hipfner et al., 2002)

Scer\GAL4^hs.2sev, ex^UAS.cBa has ommatidium phenotype, enhanceable by +/Df(3L)mir-ban^Δ1

(Hipfner et al., 2002)

NOT Enhanced by

Statement

Reference

Scer\GAL4^hs.2sev, ex^UAS.cBa has eye phenotype, non-enhanceable by Cdk4³/Cdk4[+]

(Hipfner et al., 2002)

Suppressed by

Statement

Reference

Scer\GAL4^GMR.PU, ex^UAS.cBa has eye | adult stage phenotype, suppressible | partially by Zyx^{UAS.Tag:Myr(Src42A)}/Scer\GAL4^GMR.PU

(Gaspar et al., 2015)

Scer\GAL4^nub.PU, ex^UAS.cBa has wing blade phenotype, suppressible by Scer\GAL4^nub.PU/Zyx^UAS.cGa

(Gaspar et al., 2015)

Scer\GAL4^GMR.PF, ex^UAS.cBa has eye disc | third instar larval stage phenotype, suppressible | partially by sd^JF02514/Scer\GAL4^GMR.PF

(Koontz et al., 2013)

Scer\GAL4^GMR.PF, ex^UAS.cBa has eye disc | third instar larval stage phenotype, suppressible | partially by Tgi^GD7081/Scer\GAL4^GMR.PF

(Koontz et al., 2013)

Scer\GAL4^GMR.PF, ex^UAS.cBa has eye disc morphogenetic furrow | third instar larval stage phenotype, suppressible | partially by sd^JF02514/Scer\GAL4^GMR.PF

(Koontz et al., 2013)

Scer\GAL4^GMR.PF, ex^UAS.cBa has eye disc morphogenetic furrow | third instar larval stage phenotype, suppressible | partially by Tgi^GD7081/Scer\GAL4^GMR.PF

(Koontz et al., 2013)

Scer\GAL4^FRT.Act5C, ex^UAS.cBa has wing disc | somatic clone | larval stage phenotype, suppressible by wts^x1

(Cho et al., 2006)

Scer\GAL4^FRT.Act5C, ex^UAS.cBa has wing disc | somatic clone | larval stage phenotype, suppressible by BacA\p35^UAS.cHa, Scer\GAL4^FRT.Act5C

(Cho et al., 2006)

Scer\GAL4^GMR.PF, ex^UAS.cBa has interommatidial cell | pupal stage phenotype, suppressible by hpo^42-47/hpo^42-47

(Hamaratoglu et al., 2006)

Scer\GAL4^GMR.PF, ex^UAS.cBa has interommatidial cell | pupal stage phenotype, suppressible by wts^x1/wts^x1

(Hamaratoglu et al., 2006)

Scer\GAL4^GMR.PF, ex^UAS.cBa has eye disc | third instar larval stage phenotype, suppressible | partially by hpo^42-47/hpo^42-47

(Hamaratoglu et al., 2006)

Scer\GAL4^GMR.PF, ex^UAS.cBa has eye disc | third instar larval stage phenotype, suppressible | partially by wts^x1/wts^x1

(Hamaratoglu et al., 2006)

Scer\GAL4^GMR.PF, ex^UAS.cBa has interommatidial cell | pupal stage phenotype, suppressible | partially by BacA\p35^UAS.cHa

(Hamaratoglu et al., 2006)

Scer\GAL4^hs.2sev, ex^UAS.cBa has eye phenotype, suppressible | partially by mir-278^UAS.cTa/Scer\GAL4^hs.2sev

(Teleman and Cohen, 2006)

Scer\GAL4^hs.2sev, ex^UAS.cBa has eye phenotype, suppressible by mir-ban^EP3622/Scer\GAL4^hs.2sev

(Hipfner et al., 2002)

Scer\GAL4^hs.2sev, ex^UAS.cBa has eye phenotype, suppressible | partially by CycD^UAS.cMa/Cdk4^UAS.cMa, Scer\GAL4^hs.2sev

(Hipfner et al., 2002)

Scer\GAL4^hs.2sev, ex^UAS.cBa has ommatidium phenotype, suppressible by mir-ban^EP3622/Scer\GAL4^hs.2sev

(Hipfner et al., 2002)

Scer\GAL4^hs.2sev, ex^UAS.cBa has eye phenotype, suppressible by BacA\p35^UAS.cHa, Scer\GAL4^hs.2sev

(Blaumueller and Mlodzik, 2000)

Scer\GAL4^hs.2sev, ex^UAS.cBa has ommatidium phenotype, suppressible by BacA\p35^UAS.cHa, Scer\GAL4^hs.2sev

(Blaumueller and Mlodzik, 2000)

Scer\GAL4^hs.2sev, ex^UAS.cBa has pigment cell phenotype, suppressible by BacA\p35^UAS.cHa, Scer\GAL4^hs.2sev

(Blaumueller and Mlodzik, 2000)

Scer\GAL4^hs.2sev, ex^UAS.cBa has eye photoreceptor cell phenotype, suppressible by BacA\p35^UAS.cHa, Scer\GAL4^hs.2sev

(Blaumueller and Mlodzik, 2000)

NOT suppressed by

Statement

Reference

Scer\GAL4^hs.2sev, ex^UAS.cBa has eye phenotype, non-suppressible by Cdk4³/Cdk4[+]

(Hipfner et al., 2002)

Suppressor of

Statement

Reference

Scer\GAL4^Act.PU/ex^UAS.cBa is a suppressor of head | somatic clone - Minute background phenotype of Df(3L)H99, mop^sfv3

(Gilbert et al., 2011)

Scer\GAL4^C855a/ex^UAS.cBa is a suppressor of embryonic/larval optic lobe phenotype of l(3)mbt^GM76

(Richter et al., 2011)

ex^UAS.cBa/Scer\GAL4^dpp.PU is a suppressor | partially of wing disc phenotype of Scer\GAL4^dpp.PU, dia^{CA.UAS.Tag:HA}

(Sansores-Garcia et al., 2011)

hpo^UAS.Tag:FLAG, ex^UAS.cBa, Scer\GAL4^Act5C.PI, wts^UAS.Tag:MYC is a suppressor of wing pouch | somatic clone | larval stage phenotype of Scer\GAL4^Act5C.PI, yki^{S111A.S168A.S250A.UAS.Tag:V5}

(Oh et al., 2009)

hpo^UAS.Tag:FLAG, ex^UAS.cBa, wts^UAS.Tag:MYC, Scer\GAL4^GMR.PU is a suppressor of eye disc | larval stage phenotype of Scer\GAL4^GMR.PU, yki^{S111A.S168A.S250A.UAS.Tag:V5}

(Oh et al., 2009)

hpo^UAS.Tag:FLAG, ex^UAS.cBa, Scer\GAL4^GMR.PU is a suppressor | partially of eye disc | larval stage phenotype of Scer\GAL4^GMR.PU, yki^{S111A.S168A.S250A.UAS.Tag:V5}

(Oh et al., 2009)

ex^UAS.cBa/Scer\GAL4^GMR.PF is a suppressor of eye | somatic clone phenotype of ft⁴²²

(Willecke et al., 2006)

ex^UAS.cBa/Scer\GAL4^GMR.PF is a suppressor of interommatidial bristle | somatic clone phenotype of ft⁴²²

(Willecke et al., 2006)

ex^UAS.cBa/Scer\GAL4^GMR.PF is a suppressor of pigment cell | somatic clone phenotype of ft⁴²²

(Willecke et al., 2006)

NOT Suppressor of

Statement

Reference

ex^UAS.cBa/Scer\GAL4^GMR.PU is a non-suppressor of eye disc | larval stage phenotype of Scer\GAL4^GMR.PU, yki^{S111A.S168A.S250A.UAS.Tag:V5}

(Oh et al., 2009)

ex^UAS.cBa, wts^UAS.Tag:MYC, Scer\GAL4^GMR.PU is a non-suppressor of eye disc | larval stage phenotype of Scer\GAL4^GMR.PU, yki^{S111A.S168A.S250A.UAS.Tag:V5}

(Oh et al., 2009)

ex^UAS.cBa/Scer\GAL4^GMR.PF is a non-suppressor of adult antennal segment | somatic clone phenotype of ft⁴²²

(Willecke et al., 2006)

ex^UAS.cBa/Scer\GAL4^GMR.PF is a non-suppressor of adult thorax | somatic clone phenotype of ft⁴²²

(Willecke et al., 2006)

ex^UAS.cBa/Scer\GAL4^GMR.PF is a non-suppressor of wing | somatic clone phenotype of ft⁴²²

(Willecke et al., 2006)

ex^UAS.cBa/Scer\GAL4^GMR.PF is a non-suppressor of leg | somatic clone phenotype of ft⁴²²

(Willecke et al., 2006)

ex^UAS.cBa/Scer\GAL4^GMR.PF is a non-suppressor of adult head | somatic clone phenotype of ft⁴²²

(Willecke et al., 2006)

ex^UAS.cBa/Scer\GAL4^GMR.PF is a non-suppressor of vibrissal bristle | somatic clone phenotype of ft⁴²²

(Willecke et al., 2006)

Additional Comments

Genetic Interactions

Statement

Reference

The severely undergrown wing phenotype seen in adult flies expressing ex^Scer\UAS.cBa under the control of Scer\GAL4^nub.PU can be strongly alleviated by co-expression of Zyx^Scer\UAS.cGa.

The small eye phenotype seen in adult flies expressing ex^Scer\UAS.cBa under the control of Scer\GAL4^GMR.PU can be strongly alleviated by co-expression of Zyx^{Scer\UAS.T:Myr-Src42A}.

The severely undergrown wing phenotype seen in adult flies expressing ex^Scer\UAS.cBa under the control of Scer\GAL4^nub.PU cannot be suppressed by co-expression of Zyx^{Δ208-231.Scer\UAS} : the wings of flies expressing both ex^Scer\UAS.cBa and Zyx^{Δ208-231.Scer\UAS} are even smaller than those of animals expressing ex^Scer\UAS.cBa alone, the wing blade being lost altogether.

(Gaspar et al., 2015)

Expression of sd^JF02514 (co-expressed with Dcr-2^Scer\UAS.cDa) significantly suppresses the morphogenetic furrow cell death seen when ex^Scer\UAS.cBa is expressed under the control of Scer\GAL4^GMR.PF.

Expression of Tgi^GD7081 (co-expressed with Dcr-2^Scer\UAS.cDa) significantly suppresses the morphogenetic furrow cell death seen when ex^Scer\UAS.cBa is expressed under the control of Scer\GAL4^GMR.PF.

(Koontz et al., 2013)

Co-expression of ex^Scer\UAS.cBa partially suppresses the F-actin accumulation caused by expression of cpa^{dsRNA.Scer\UAS.cFa} under the control of Scer\GAL4^hh-Gal4 from the early third larval instar onwards (the temperature sensitive Scer\GAL80^ts.αTub84B allele is used to restrict expression before this stage).

(Fernández et al., 2011)

Expression of ex[Scer\UAS.cBa] under the control of Scer\GAL4^Act.PU suppresses the head overgrowth seen in mop^sfv3 Df(3L)H99 mutant clones (generated in a minute background).

(Gilbert et al., 2011)

Expression of ex^Scer\UAS.cBa under the control of Scer\GAL4^C855a suppresses the increase in volume of the optic lobe neuroepithelium which is seen in l(3)mbt^GM76 homozygotes.

(Richter et al., 2011)

Co-expression of ex^Scer\UAS.cBa in clones expressing yki^{S168A.Scer\UAS.T:SV5\V5}, wts^{Scer\UAS.T:Hsap\MYC} and hpo^{Scer\UAS.T:Zzzz\FLAG} under the control of Scer\GAL4^Act5C.PI decreases the size and number of clones recovered in the wing disc.

Clones co-expressing yki^{S111A.S168A.S250A.Scer\UAS.T:SV5\V5} ex^Scer\UAS.cBa, wts^{Scer\UAS.T:Hsap\MYC} and hpo^{Scer\UAS.T:Zzzz\FLAG} under the control of Scer\GAL4^Act5C.PI in the wing disc are large and round.

(Oh and Irvine, 2009)

Clones of cells in the wing or eye disc in which wts^{Scer\UAS.T:Hsap\MYC}, hpo^{Scer\UAS.T:Zzzz\FLAG} and ex^Scer\UAS.cBa are over-expressed together with yki^{S111A.S168A.S250A.Scer\UAS.T:SV5\V5} generally overgrow compared to control clones, but can be less overgrown than yki^{S111A.S168A.S250A.Scer\UAS.T:SV5\V5}-expressing clones alone. This is most evident in the wing pouch.

The overgrowth and folding of the eye disc induced by Scer\GAL4^GMR.PU-mediated expression of yki^{S111A.S168A.S250A.Scer\UAS.T:SV5\V5} is largely suppressed by co-expression of wts^{Scer\UAS.T:Hsap\MYC}, hpo^{Scer\UAS.T:Zzzz\FLAG} and ex^Scer\UAS.cBa.

The overgrowth and folding of the eye disc induced by Scer\GAL4^GMR.PU-mediated expression of yki^{S111A.S168A.S250A.Scer\UAS.T:SV5\V5} is not suppressed by co-expression of ex^Scer\UAS.cBa alone, nor by co-expression of wts^{Scer\UAS.T:Hsap\MYC} and ex^Scer\UAS.cBa together.

The overgrowth and folding of the eye disc induced by Scer\GAL4^GMR.PU-mediated expression of yki^{S111A.S168A.S250A.Scer\UAS.T:SV5\V5} is partly suppressed by co-expression of hpo^{Scer\UAS.T:Zzzz\FLAG} and ex^Scer\UAS.cBa together.

(Oh et al., 2009)

Eye clones of ft⁸ misexpressing ex^Scer\UAS.cBa, under the control of Scer\GAL4^GMR.PF at 25^oC exhibit small eyes, indicating that the small-eye phenotype produced by misexpressing ex^Scer\UAS.cBa is epistatic to the eye size resulting from ft⁸.

In eye-discs mosaic for ft⁸, the expression ex^Scer\UAS.cBa under the control of Scer\GAL4^GMR.PF induces apoptosis as in wild-type tissue.

(Bennett and Harvey, 2006)

A wts^x1 mutant background allows recovery of larval wing disc clones expressing ex^Scer\UAS.cBa under the control of Scer\GAL4^{Scer\FRT.Act5C}.

(Cho et al., 2006)

The loss of inter-ommatidial cells in the developing retinas of ex^Scer\UAS.cBa; Scer\GAL4^GMR.PF animals at the mid-pupal stage is completely suppressed by hpo^42-47/hpo^42-47 or in wts^x1/wts^x1 somatic clones. In each case, the resulting retinas have an excess of inter-ommatidial cells as do wts^x1/wts^x1 or hpo^42-47/hpo^42-47 animals. Heterozygosity for wts^x1 partially suppresses the reduction in size and patterning defects seen in the eyes of ex^Scer\UAS.cBa; Scer\GAL4^GMR.PF animals.

The increase in cell death the late third instar eye discs of ex^Scer\UAS.cBa; Scer\GAL4^GMR.PF animals is largely suppressed by wts^x1/wts^x1 or hpo^42-47/hpo^42-47.

(Hamaratoglu et al., 2006)

Overexpression of ex^Scer\UAS.cBa in the developing eyes of ft⁴²² mutant heads, under the control of Scer\GAL4^GMR.PF results in small and rough eyes, very similar to the overexpression of ex^Scer\UAS.cBa in a wild-type background, indicating that ex^Scer\UAS.cBa expression suppresses the ft⁴²² mutant eye phenotype. ft⁴²² mutant phenotypes, such as extra vibrassae, are still observed in the head cuticle, where ex^Scer\UAS.cBa is not overexpressed (under the control of Scer\GAL4^GMR.PF).

(Willecke et al., 2006)

Co-expression of ban^EP3622 almost completely suppresses the eye phenotype caused by expression of ex^Scer\UAS.cBa under the control of Scer\GAL4^hs.2sev. The eye phenotype caused by expression of ex^Scer\UAS.cBa under the control of Scer\GAL4^hs.2sev is enhanced by Df(3L)ban^Δ1/+. Co-expression of both CycD^Scer\UAS.cMa and Cdk4^Scer\UAS.cMa in animals expressing ex^Scer\UAS.cBa under the control of Scer\GAL4^hs.2sev increases the overall size of the eye but has little effect on the roughness and blistering. The eye phenotype caused by expression of ex^Scer\UAS.cBa under the control of Scer\GAL4^hs.2sev is not altered by Cdk4³/+.

(Hipfner et al., 2002)

Addition of BacA\p35^Scer\UAS.cHa to ex^Scer\UAS.cBa, Scer\GAL4^hs.2sev flies rescues the phenotypes seen in ex^Scer\UAS.cBa, Scer\GAL4^hs.2sev flies alone. Externally the eye is much smoother and in sections most of the cell loss is rescued, although occasional photoreceptor and pigment cell loss is evident. Defects in planar polarity are still prominent.

(Blaumueller and Mlodzik, 2000)

Xenogenetic Interactions

Statement

Reference

Co-expression of BacA\p35^Scer\UAS.cHa allows recovery of larval wing disc clones expressing ex^Scer\UAS.cBa under the control of Scer\GAL4^{Scer\FRT.Act5C}.

(Cho et al., 2006)

The loss of inter-ommatidial cells in the developing retinas of ex^Scer\UAS.cBa; Scer\GAL4^GMR.PF animals at the mid-pupal stage is only partially suppressed by BacA\p35^Scer\UAS.cHa.

(Hamaratoglu et al., 2006)

Complementation and Rescue Data

Rescues

Scer\GAL4^32B/ex^UAS.cBa rescues ex^e1

(Boedigheimer et al., 1997)

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

600515

w^*; P{UAS-ex.B}12.5

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

ex^Scer\UAS.cBa

ex^UAS.cBa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Boedigheimer

(Boedigheimer et al., 1997)

Secondary FlyBase IDs

References (24)

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