FlyBase Allele Report: Dmel\spn-A[1]

General Information

Symbol

Dmel\spn-A¹

Species

D. melanogaster

Name

FlyBase ID

FBal0016029

Feature type

allele

Associated gene

Dmel\spn-A

Associated Insertion(s)

Carried in Construct

Also Known As

spnA¹, spn-A⁰⁰³, rad51¹

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

(Staeva-Vieira et al., 2003, Lindsley and Zimm, 1992, Tearle and Nusslein-Volhard, 1987)

Progenitor genotype

Cytology

Description

Amino acid replacement: G149D.

(Staeva-Vieira et al., 2003)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

3R:30,055,431..30,055,431 [-]

Nucleotide change:

G30055431A

Amino acid change:

G149D | spn-A-PA; G92D | spn-A-PB

Reported amino acid change:

G149D

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(Staeva-Vieira et al., 2003)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal DNA repair

(Wei and Rong, 2007)

decreased fecundity | female

(Lindsley and Zimm, 1992)

lethal | maternal effect

(Lindsley and Zimm, 1992)

radiation sensitive | dominant

(Holmes et al., 2006)

viable (with Df(3R)X3F)

(Staeva-Vieira et al., 2003)

Phenotype Manifest In

chromosome (with Df(3R)X3F)

(Beaucher et al., 2012)

chromosome | third instar larval stage

(Merigliano et al., 2019)

dorsal appendage

(Lindsley and Zimm, 1992)

dorsal appendage (with spn-A^093A)

(Bayer et al., 2020)

dorsal appendage | maternal effect (with spn-A^093A)

(Ferguson et al., 2012)

egg

(Lindsley and Zimm, 1992)

egg chorion (with spn-A^093A)

(Barbosa et al., 2007)

embryonic/larval brain | third instar larval stage

(Merigliano et al., 2019)

karyosome

(Nagel et al., 2012)

karyosome | adult stage

(Zhaunova et al., 2016)

neuroblast | third instar larval stage

(Merigliano et al., 2019)

presumptive oocyte

(Joyce and McKim, 2009)

Detailed Description

Statement

Reference

spn-A^093A/spn-A¹ but not spn-A¹/+ females lay eggs with defective dorsal appendages (fused, narrowly spaced or missing) when compared to controls.

(Bayer et al., 2020)

spn-A¹ homozygous third instar larval brain neuroblasts show a small increase in the frequency of chromosomal aberrations, as compared to controls.

(Merigliano et al., 2019)

spn-A¹/spn-A¹ third instar larval brains exhibit a similar frequency of cells with chromosome aberrations and no telomeric fusions compared to controls.

(Merigliano et al., 2017)

spn-A¹ mutant female ovarioles exhibit abnormal karyosome morphology, as compared to controls.

(Zhaunova et al., 2016)

Eggs laid by spn-A^093A/spn-A¹ females display a range of ventralisation phenotypes, ranging from fusion at the base of the dorsal appendages through to complete lack of the appendages.

(Ferguson et al., 2012)

Mutant oocytes show defects in karyosome morphology. The DNA is thread-like or oblong in 68% of egg chambers and 27% of egg chambers show a distorted oocyte nuclear membrane.

(Nagel et al., 2012)

spn-A¹ mutants exhibit an accumulation of γHis2Av foci that persist through meiotic prophase, corresponding to unrepaired meiotic double-strand breaks. A mean of 22.8 γ-His2Av foci is present in spn-A¹ region 3 oocytes, which is similar to estimates for the total number of double strand breaks per nucleus.

(Joyce et al., 2011)

Homozygous females show a high frequency (approximately 70%) of region 3 cysts with two pro-oocytes (as assayed by c(3)G staining) compared to a frequency of only 9.5% in wild type.

(Joyce and McKim, 2009)

spn-A¹/spn-A^093A females produce eggs with ventralised eggshells.

(Barbosa et al., 2007)

spn-A¹/spn-A¹ and spn-A¹/Df(3R)X3F mutants have a significantly elevated frequency of single-strand annealing repair (SSA) compared to controls in a P{wIw.FRT} hemizygous assay to study DNA double-stranded break repair.

spn-A¹/Df(3R)X3F mutants have a significantly elevated frequency of single-strand annealing repair (SSA) compared to controls in a P{wIw.FRT}/P{wIw.FRT.8z} homozygous assay to study DNA double-stranded break repair, while interhomolog gene conversion (GC) is abolished.

(Wei and Rong, 2007)

Heterozygotes show a reduced survival rate following irradiation compared to control animals.

(Holmes et al., 2006)

Egg shape affected; in extreme cases dorsal appendages are lacking and eggs have little or no dorsal-ventral polarity; some eggs have one fused dorsal appendage. Low fecundity; eggs often slightly collapsed.

(Lindsley and Zimm, 1992)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Other

Statement

Reference

spn-A¹, tws⁴³⁰ has lethal - all die before end of larval stage phenotype

(Merigliano et al., 2017)

Blm^D2, mus81^5.1, spn-A¹ has viable phenotype

(Johnson-Schlitz and Engels, 2006)

Phenotype Manifest In

Enhanced by

Statement

Reference

spn-A¹ has embryonic/larval brain | third instar larval stage phenotype, enhanceable by aurA⁹⁴⁹/aurA⁹⁴⁹

(Merigliano et al., 2019)

spn-A¹ has neuroblast | third instar larval stage phenotype, enhanceable by aurA⁹⁴⁹/aurA⁹⁴⁹

(Merigliano et al., 2019)

spn-A¹ has FlyBase_internalproperty type:chromosome:chromosome | third instar larval stage phenotype, enhanceable by aurA⁹⁴⁹/aurA⁹⁴⁹

(Merigliano et al., 2019)

NOT Enhanced by

Statement

Reference

spn-A^093A/spn-A¹ has dorsal appendage phenotype, non-enhanceable by Xrcc2^CC/Xrcc2^CC

(Bayer et al., 2020)

Suppressed by

Statement

Reference

spn-A¹ has karyosome | adult stage phenotype, suppressible by lok[+]/lok^p6

(Zhaunova et al., 2016)

spn-A^093A/spn-A¹ has dorsal appendage | maternal effect phenotype, suppressible by Lnk^d07478/Lnk^CR642

(Ferguson et al., 2012)

spn-A¹ has presumptive oocyte phenotype, suppressible by rec²

(Joyce and McKim, 2009)

spn-A^093A/spn-A¹ has egg chorion phenotype, suppressible by mei-W68¹/mei-W68¹

(Barbosa et al., 2007)

NOT suppressed by

Statement

Reference

spn-A^093A/spn-A¹ has dorsal appendage phenotype, non-suppressible by Xrcc2^CC/Xrcc2^CC

(Bayer et al., 2020)

spn-A¹ has presumptive oocyte phenotype, non-suppressible by mei-P22¹⁰³

(Joyce and McKim, 2009)

Enhancer of

Statement

Reference

spn-A¹/spn-A¹ is an enhancer of embryonic/larval brain | third instar larval stage phenotype of aurA⁹⁴⁹

(Merigliano et al., 2019)

spn-A¹/spn-A¹ is an enhancer of neuroblast | third instar larval stage phenotype of aurA⁹⁴⁹

(Merigliano et al., 2019)

spn-A¹/spn-A¹ is an enhancer of FlyBase_internalproperty type:chromosome:chromosome | third instar larval stage phenotype of aurA⁹⁴⁹

(Merigliano et al., 2019)

Suppressor of

Statement

Reference

spn-A¹/spn-A¹ is a suppressor | partially of nuclear chromosome | third instar larval stage phenotype of tws⁴³⁰

(Merigliano et al., 2017)

spn-A¹/spn-A¹ is a suppressor | partially of embryonic/larval brain | third instar larval stage phenotype of tws⁴³⁰

(Merigliano et al., 2017)

Other

Statement

Reference

Xrcc2^CC, spn-A¹ has dorsal appendage phenotype

(Bayer et al., 2020)

Additional Comments

Genetic Interactions

Statement

Reference

Xrcc2^CC homozygous females in the heterozygous background of spn-A¹ lay eggs with defective dorsal appendages (fused, narrowly spaced, branched or shortened) when compared to controls.

(Bayer et al., 2020)

A very small proportion of Pol32^L30/Pol32^L27 adults show an etched abdomen phenotype; this phenotype is not much affected in a spn-A¹/+ background.

(Ji et al., 2019)

lok^p6/+ suppresses the abnormal karyosome morphology observed in spn-A¹ mutants.

(Zhaunova et al., 2016)

The high frequency of region 3 cysts containing two pro-oocytes that is seen in spn-A¹ homozygous females is suppressed by rec² but is not suppressed by mei-P22¹⁰³.

(Joyce and McKim, 2009)

The ventralised eggshell phenotype produced by spn-A¹/spn-A^093A females is suppressed by mei-W68¹.

(Barbosa et al., 2007)

The triple mutant of mus81^5.1, mus309^D2 and spn-A¹ is lethal.

The elevated recombination caused by mus309^D2 is abolished by spn-A¹.

(Johnson-Schlitz and Engels, 2006)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

3322

Dp(1;Y)Bar^S; ru¹ st¹ e¹ spn-A¹ ca¹/TM3, Sb¹

Notes on Origin

Discoverer

Comments

Weak mutation.

(Gonzalez-Reyes et al., 1997)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (7)

Reported As

Symbol Synonym

003

(Staeva-Vieira et al., 2003)

(Johnson-Schlitz et al., 2007)

rad51¹

(Porrazzo et al., 2022, Merigliano et al., 2019, Merigliano et al., 2017)

spn-A⁰⁰³

(Bayer et al., 2020, Ferguson et al., 2012, Gonzalez-Reyes et al., 1997, Lindsley and Zimm, 1992, Tearle and Nusslein-Volhard, 1987)

spn-A¹

(Nagel et al., 2012, Joyce et al., 2011, Joyce and McKim, 2010, Joyce and McKim, 2009, Barbosa et al., 2007)

spnA⁰⁰³

(Yoo and McKee, 2005)

spnA¹

(Ji et al., 2019, Wei et al., 2019, Zhaunova et al., 2016, Breuer and Ohkura, 2015, Molla-Herman et al., 2015, Beaucher et al., 2012, Wei and Rong, 2007, Holmes et al., 2006, Johnson-Schlitz and Engels, 2006)

Name Synonyms

Secondary FlyBase IDs

References (27)

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