FlyBase Allele Report: Dmel\trol[13]

General Information

Symbol

Dmel\trol¹³

Species

D. melanogaster

Name

FlyBase ID

FBal0017058

Feature type

allele

Associated gene

Dmel\trol

Associated Insertion(s)

Carried in Construct

Also Known As

trol^b22, l(1)zw1^b22

Key Links

Allele class

hypomorphic allele - genetic evidence

Mutagen

X ray

Nature of the Allele

Allele class

hypomorphic allele - genetic evidence

(Park et al., 2003)

Mutagen

X ray

Progenitor genotype

Cytology

Polytene chromosomes normal (Judd).

Description

Single nucleotide deletion at position 1181 of the putative mRNA results in a frameshift. This is predicted to reduce the size of the protein product from 450kD to 400kD.

(Park et al., 2003)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal | recessive

(Robbins, 1983, Liu and Lim, 1975, Judd et al., 1972, Shannon et al., 1972)

partially lethal - majority die | recessive

(Park et al., 1998)

viable (with trol^d8)

(Park et al., 2001)

viable (with trol^p4)

(Park et al., 2001)

Phenotype Manifest In

larval brain & neuroblast

(Park et al., 1998)

Detailed Description

Statement

Reference

trol¹³ homozygotes have normal numbers of S phase larval neuroblasts per brain lobe.

(Park et al., 2003)

trol¹³/Y males are about 71% viable compared to wild-type males at 25^oC. Hemizygous trol¹³ male larvae have a normal number of S phase neuroblasts per brain lobe.

(Park et al., 1998)

Normal larval brain histology. Male adult escapers have a roughened eye phenotype: irregular packing and size of ommatidia, aberrant bristle placement and number. Gynandromorph analysis shows the brain phenotype is due to misrotation of the optic lobes and misrouted retinula or lamina fibres. Hemizygous males and homozygous females show reduced flight/escape response.

(Datta and Kankel, 1992)

Lethality occurs during larval and pupal stages. The growth rate begins to decrease during the second larval instar. Mosaic analysis demonstrates mutant phenotype is non-autonomous.

(Shannon et al., 1972)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

trol¹³ has decreased occurrence of cell division | larval stage phenotype, enhanceable by ttv[+]/ttv^00681b

(Park et al., 2003)

trol¹³ has lethal phenotype, enhanceable by eve^10-5/eve[+]

(Park et al., 2001)

trol¹³ has lethal phenotype, enhanceable by eve[+]/eve^20-35

(Park et al., 2001)

trol¹³ has lethal phenotype, enhanceable by eve^58-11/eve[+]

(Park et al., 2001)

trol¹³ has partially lethal - majority die phenotype, enhanceable by +/Df(2R)X1

(Park et al., 1998)

trol¹³ has partially lethal - majority die phenotype, enhanceable by Df(2R)B5/+

(Park et al., 1998)

trol¹³ has partially lethal - majority die phenotype, enhanceable by Df(2R)eve/+

(Park et al., 1998)

trol¹³ has partially lethal - majority die phenotype, enhanceable by eve[+]/eve⁵

(Park et al., 1998)

trol¹³ has partially lethal - majority die phenotype, enhanceable by eve[+]/eve¹

(Park et al., 1998)

trol¹³ has partially lethal - majority die phenotype, enhanceable by eve³/eve[+]

(Park et al., 1998)

trol¹³ has partially lethal - majority die phenotype, enhanceable by eve[+]/eve⁴

(Park et al., 1998)

trol¹³ has partially lethal - majority die phenotype, enhanceable by eve^tPa

(Park et al., 1998)

NOT Enhanced by

Statement

Reference

trol¹³ has lethal phenotype, non-enhanceable by eve^14-10/eve[+]

(Park et al., 2001)

trol¹³ has partially lethal - majority die phenotype, non-enhanceable by Df(2R)stan2

(Park et al., 1998)

Suppressed by

Statement

Reference

eve³/eve[+], trol¹³ has decreased occurrence of cell division | third instar larval stage phenotype, suppressible | partially by CycE^hs.PR

(Park et al., 2001)

NOT suppressed by

Statement

Reference

eve³/eve[+], trol¹³ has lethal phenotype, non-suppressible by CycE^hs.PR

(Park et al., 2001)

Other

Statement

Reference

hh[+]/hh²¹, trol¹³ has decreased occurrence of cell division | larval stage phenotype

(Park et al., 2003)

btl^LG19/btl[+], trol¹³ has decreased occurrence of cell division | larval stage phenotype

(Park et al., 2003)

hh[+]/hh², trol¹³ has decreased occurrence of cell division | larval stage phenotype

(Park et al., 2003)

bnl⁰⁶⁹¹⁶/bnl[+], trol¹³ has decreased occurrence of cell division | larval stage phenotype

(Park et al., 2003)

hh[+]/hh^ts2, trol¹³ has decreased occurrence of cell division | larval stage | temperature conditional phenotype

(Park et al., 2003)

eve³/eve[+], trol¹³ has lethal phenotype

(Park et al., 2001)

eve³/eve[+], trol¹³ has decreased occurrence of cell division | third instar larval stage phenotype

(Park et al., 2001)

eve³/eve[+], trol¹³ has decreased occurrence of cell division | larval stage phenotype

(Park et al., 1998)

eve[+]/eve⁴, trol¹³ has decreased occurrence of cell division | larval stage phenotype

(Park et al., 1998)

Phenotype Manifest In

Enhanced by

Statement

Reference

trol¹³ has neuroblast | larval stage phenotype, enhanceable by btl^LG19/btl[+]

(Park et al., 2003)

trol¹³ has neuroblast | larval stage phenotype, enhanceable by hh[+]/hh²

(Park et al., 2003)

trol¹³ has neuroblast | larval stage phenotype, enhanceable by hh[+]/hh^AC

(Park et al., 2003)

trol¹³ has neuroblast | larval stage phenotype, enhanceable by bnl⁰⁶⁹¹⁶/bnl[+]

(Park et al., 2003)

trol¹³ has phenotype, enhanceable by eve⁶

(Park and Datta, 1998)

trol¹³ has phenotype, enhanceable by eve⁵

(Park and Datta, 1998)

NOT Enhanced by

Statement

Reference

trol¹³ has neuroblast | larval stage phenotype, non-enhanceable by htl^AB42/htl[+]

(Park et al., 2003)

Suppressed by

Statement

Reference

eve³/eve[+], trol¹³ has larval brain & neuroblast phenotype, suppressible by CycE^hs.PR

(Park et al., 2001)

Suppressor of

Statement

Reference

trol¹³/trol¹³ is a suppressor of neuroblast | larval stage | heat sensitive phenotype of hh^ts2

(Park et al., 2003)

Other

Statement

Reference

hh[+]/hh²¹, trol¹³ has neuroblast | larval stage phenotype

(Park et al., 2003)

trol¹³, ttv^00681b has neuroblast | larval stage phenotype

(Park et al., 2003)

hh²¹, trol¹³ has neuroblast | larval stage phenotype

(Park et al., 2003)

eve³, trol¹³ has larval brain & neuroblast phenotype

(Park et al., 2001, Park et al., 1998)

eve³/eve[+], trol¹³ has larval brain & neuroblast phenotype

(Park et al., 2001)

eve⁴, trol¹³ has larval brain & neuroblast phenotype

(Park et al., 1998)

eve⁵, trol¹³ has larval brain & neuroblast phenotype

(Park et al., 1998)

Additional Comments

Genetic Interactions

Statement

Reference

trol¹³/trol¹³; bnl⁰⁶⁹¹⁶/+ and trol¹³/trol¹³; btl^LG19/+ larvae, but not trol¹³/trol¹³; htl^AB42/+ larvae have reduced numbers S phase neuroblasts per brain lobe compared to trol¹³ homozygotes. trol¹³/trol¹³; larvae, heterozygous for hh²or hh^AC have reduced numbers S phase neuroblasts per brain lobe compared to trol¹³ homozygotes. trol¹³/trol¹³; heterozygous for hh²¹ have increased numbers of S phase neuroblasts per brain lobe compared to trol¹³ homozygotes.

(Park et al., 2003)

96% of trol¹³/Y ; eve³/+ animals show defective proliferation of larval brain neuroblasts. The addition of CycE^hs.PR (without heat shock) reduces the number of animals with defective proliferation to 35%, and expression of CycE^hs.PR using a 30 minute heat shock rescues the neuroblast phenotype almost completely. Weak induction of CycE^hs.PR does not rescue the enhanced lethality of trol¹³/Y ; eve³/+ animals. trol¹³/Y ; eve³/+ brains cultured in an explant assay in larval extract from eve³/+ or trol¹³/Y ; eve³/+ animals show a reduced amount of proliferation compared to wild-type brains cultured in larval extract from wild-type animals. In contrast, trol¹³/Y ; eve³/+ brains cultured in larval extract from wild-type animals have a level of proliferation close to that seen in controls (wild-type brains cultured in larval extract from wild-type animals). No trol¹³ brains cultured in larval extract from eve³/+ animals show normal proliferation. Addition of ecdysone rescues defective proliferation in trol¹³/Y ; eve³/+ brains cultured in larval extract from trol¹³/Y ; eve³/+ animals.

(Park et al., 2001)

The trol¹³/Y semi-lethality is dominantly enhanced by Df(2R)X1, Df(2R)B5 or Df(2R)eve. These deficiences also show a strong transheterozygous effect in trol¹³/+ females, resulting in a reduction in viability. The semi-lethality is not dominantly enhanced by Df(2R)stan2. The semi-lethality of trol¹³/Y males is also dominantly enhanced by eve⁵, eve¹, eve³ and eve⁴ and these alleles also show a transheterozygous effect in trol¹³/+ females, resulting in a reduction in viability. trol¹³/Y males carrying eve^tPa show reduced viability compared to control siblings. If the trol¹³ larvae are also heterozygous for either eve³ or eve⁴ then the number of S phase neuroblasts per brain lobe is reduced. If the trol¹³ larvae are also heterozygous for eve⁵, then the number of S phase neuroblasts per brain lobe is reduced in 66% of larvae and unaffected in 33% of larvae.

(Park et al., 1998)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

trol^G0374

(Voigt et al., 2002)

trol^G0271

(Voigt et al., 2002)

trol^G0023

(Voigt et al., 2002)

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

4284

trol¹³/FM7a/Dp(1;2;Y)w⁺

Kyoto

107669

trol¹³/FM7a/Dp(1;2;Y)w⁺

Notes on Origin

Discoverer

Judd, 22 Feb. 1962.

Comments

Shows no maternal-zygotic interaction.

(Robbins, 1983)

Heteroallelic combinations are fully lethal.

(Datta and Kankel, 1992)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (8)

Reported As

Symbol Synonym

62b22

(Thierry-Mieg, 1982)

l(1)3Ac¹³

l(1)trol^b22

(Datta and Kankel, 1992)

l(1)zw1^b22

(Judd et al., 1972, Shannon et al., 1972)

trol¹³

(Jin et al., 2020, Thuveson et al., 2019, Voigt et al., 2002)

trol^b22

(Park et al., 2003, Park et al., 2001, Park et al., 1999, Park and Datta, 1998, Park et al., 1998)

zw-1^62b22

(Liu and Lim, 1975)

zw1^b22

(Robbins, 1983)

Name Synonyms

Secondary FlyBase IDs

References (15)

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