FB2024_03 , released June 25, 2024
Allele: Dmel\Ubxpbx-1
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General Information
Symbol
Dmel\Ubxpbx-1
Species
D. melanogaster
Name
FlyBase ID
FBal0017568
Feature type
allele
Associated gene
Dmel\Ubx
Associated Insertion(s)
Carried in Construct
Also Known As
pbx1, pbx
Key Links
Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
X ray
(Lindsley and Zimm, 1992, Bender et al., 1983)
Progenitor genotype
Caused by aberration
Df(3R)Ubxpbx-1
Cytology

Polytene chromosomes normal.

(Lindsley and Zimm, 1992, Castelli-Gair and Garcia-Bellido, 1990)
Description

The deletion that causes this allele also deletes regions of the neighbouring bxd non-coding RNA gene.

(Petruk et al., 2006)

Cis-regulatory mutation.

(LaJeunesse and Shearn, 1995)

Deletion of DNA in the distal part of the bxd regulatory region.

(Castelli-Gair et al., 1992)

Deletion at Ubx at -30kb with respect to transcription start site.

(Muller and Bienz, 1991)

Deletion of DNA within the pbx regulatory region.

(Castelli-Gair and Garcia-Bellido, 1990)

Deletion of DNA.

(Castelli-Gair et al., 1990, Micol et al., 1990)

Deletion in the bxd region, upstream of the Ubx transcription unit.

(Little et al., 1990)

Deletion of 17kb from map position -3 to +14kb.

(Bender et al., 1983)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      UbxLDN/Ubxabx-1 Ubxbx-3 Ubxpbx-1 adults show transformation of the distal part of the haltere (the capitellum) into the wing; the haltere is bigger than normal and has bristles on the margins and veins on the surface.

      (de Navas et al., 2011)

      The P compartment of Ubxpbx-1/Ubx9.22 haltere discs is increased in size relative to the disc, such that the P:A size ration is 1.45. This effect is not seen in Ubx9.22/+ discs.

      (Crickmore and Mann, 2006)

      The posterior compartment is increased in size in Ubx130/Ubxpbx-1 and Ubx6.28/Ubxpbx-1 haltere discs.

      (de Navas et al., 2006)

      The haltere is partially transformed to a wing in Ubxabx-1 Ubxbx-3 Ubxpbx-1/UbxLDN flies.

      (de Navas et al., 2006)

      In Ubxbx-3 Ubxpbx-1 Ubx6.28 L3 larvae, tracheal metamere 3 is transformed into tracheal metamere 2. Tracheal metamere 2 appears normal.

      (Guha and Kornberg, 2005)

      Ubx61d, Ubxpbx-1/Ubxbx-34e mutants show a haltere-to-wing transformation.

      (Ronshaugen et al., 2005)

      Ubxpbx-1/Df(3R)P2 flies show transformation of the posterior compartment of T3 (T3p) into the posterior compartment of T2 (T2p). The homeotically transformed T3 segment does not contain DVM-I, DVM-II, DVM-III, DLM or DFM muscles. Ubxabx-1 Ubxbx-3 Ubx61d Ubxpbx-1/Df(3R)P2 mutants show transformation of T3 into T2 resulting in the replacement of halteres with a second set of wings. The homeotically transformed T3 segment resembles T2 in size. The homeotically transformed T3 segment can contain homeotic DVM-I, DVM-II, DVM-III, DLM and DFM muscles.

      (Rivlin et al., 2001)

      Ubxabx-1, Ubxbx-3 and Ubxpbx-1 triple mutants cause muscle patterning defects in A1 of pupae, transforming them towards a T3 thoracic identity.

      (Roy and VijayRaghavan, 1997)

      Ubxabx-1 Ubxbx-3 Ubxpbx-1 triple mutant adults have four wings.

      (Roy et al., 1997)

      A few double heterozygotes for ash128 at 27oC exhibit haltere to wing transformation, at 20oC they also exhibit third to second leg transformation. Double heterozygotes for ash218 at 20oC exhibit very mild haltere to wing transformation.

      (LaJeunesse and Shearn, 1995)

      In Ubxabx-1 Ubxpbx-1 Ubxbx-3 triple mutant flies the T3 ectoderm transforms toward T2 identity. Transformation changes the pattern of myoblasts on the transformed haltere disc and their pattern of migration, but indirect flight muscles do not form as there are no larval templates. The haltere disc is almost completely transformed into a wing disc. Adults have two pairs of wings, but the homeotically transformed T3 has only rudimentary indirect flight muscles.

      (Fernandes et al., 1994)

      The giant fibre (GF) of Ubxabx-1 Ubxbx-3 Ubxpbx-1/Df(3R)P2 mutants makes a midline tuft and extends a lateral process to T2 and T3 segment. In the presence of ben1 the GF is capable of extending a process into the T3 segment but no lateral processes from the midline.

      (Muralidhar and Thomas, 1993)

      Flies triply mutant for Ubxabx-1, Ubxbx-3 and Ubxpbx-1 over Df(3R)P2 have a transformed metathorax including neural elements and jump muscle, that function in the escape-jump response within that segment. Corresponding transformation of the flight muscles is infrequent.

      (Schneiderman et al., 1993)

      Ubx1/Ubxpbx-1 transheterozygotes are viable, with the following transformation in the adult: T3p to T2p.

      (Castelli-Gair et al., 1992)

      Homozygotes show complete T3p to T2p ("pbx") transformation. A1a is not affected.

      (Castelli-Gair et al., 1992)

      Increases the frequency of the trx bithorax-variegated phenotype in heterozygous combination with Df(3R)red-P52.

      (Castelli-Gair and Garcia-Bellido, 1990)

      Transheterozygotes with Ubx1 show an enhancement of the bx phenotype when in combination with E(bx)2. Haltere to winglike transformation is enhanced.

      (Bhosekar and Babu, 1987)

      Different genetic combinations of Pc alleles and Ubx alleles suggest that the products of the bx region of Ubx are responsible for the "cbx" phenotype of the Pc mutations and in the absence of Pc the overexpression of bx gene products suppresses the pbx insufficiency phenotype in the posterior segments.

      (Capdevila et al., 1986)

      Homozygous flies have normal transversal fascicles in the adult CNS. Ubxpbx-1/Df(3R)P10 flies have abnormal posterior internal T3 and anterior dorsal A1 fascicles and sometimes abnormal posterior dorsal T3, and anterior internal A1 fascicles.

      (Teugels and Ghysen, 1985)

      Ubxpbx-1/Df(3R)Ubx109 flies show transformation of posterior haltere to wing and have extra dorsal postnotal tissue. These flies show variable slight reduction of the first abdominal tergite.

      (Bender et al., 1983)

      Ubxbx-3 Ubxpbx-1 flies show a transformation of haltere to wing. The transformed appendage is smaller than wild-type, the cuticle is often folded, and the veins are often obscure. The phenotype of the sensilla is similar to Ubxbx-3 homozygotes, except that the homeotic transformation in the proximal radius region is more complete, and d.Rad.D contains sensilla resembling, but not identical to, the pedicellar sensilla of the haltere. Ubxabx-1 Ubxbx-3 Ubxpbx-1 flies have a transformation of haltere to wing. The homeotic appendage is somewhat smaller than a normal wing, but usually has the full wild-type complement of wing sensilla, with each type located in the normal position and having normal morphology. The only exception is an occasional decrease in the number of sensilla in fields d.Rad.B and d.Rad.C, and rarely, one or a few extra haltere pedicellar bristles.

      (Cole and Palka, 1982)

      Hemizygous Ubxpbx-1/Df(3R)P115 tissue shows a transformation of the metathoracic segment (both dorsal and ventral discs) to the mesothoracic segment in gynandromorphs. This transformation is restricted to the posterior compartment.

      (Minana and Garcia-Bellido, 1982)

      The frequency of variegated bithorax transformations seen in Df(3R)red-P52 heterozygotes is not increased in flies also heterozygous for Ubxpbx-1.

      (Capdevila and Garcia-Bellido, 1981)

      Transforms posterior metathoracic segment into a posterior mesothoracic structure. Transformation suppressed by UbxCbx-1. Ubxbx-3 Ubxpbx-1 homozygotes show virtually complete mesothoracic transformation of the metathorax. Ubxbx-3 +/+ Ubxpbx-1 is wild type. bxd Ubxpbx-1/+ + is wild type, but Ubxbxd-1 +/+ Ubxpbx-1 shows moderate Ubxpbx-1-like transformation. RK3.

      (Lindsley and Grell, 1968)

      The posterior portion of the halteres shows a slight wing-like modification in Ubxbx-3/Ubxpbx-1 flies.

      (Lewis, 1959)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Suppressed by
      Statement
      Reference

      Ubxpbx-1/Ubx101 has viable phenotype, suppressible by xen[+]/Asxxen-72-3

      (Bischoff et al., 2009)
      Phenotype Manifest In
      Enhanced by
      Statement
      Reference

      Ubxpbx-1 has haltere phenotype, enhanceable by zae(bx)

      (Lewis, 1959)
      Suppressed by
      NOT suppressed by
      Statement
      Reference

      Ubxpbx-1 has phenotype, non-suppressible by su(Hw)2

      (Lindsley and Grell, 1968)
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Heterozygosity for Asxxen-72-3 in a Ubxpbx-1/Ubx101 mutant background causes lethality at the third instar larval stage.

      (Bischoff et al., 2009)

      Expression of tkvScer\UAS.cNa under the control of Scer\GAL4Act5C in a Ubxpbx-1/Ubx9.22 background reduces the size of the P compartment of haltere discs relative to that observed in Ubxpbx-1/Ubx9.22 discs (from 1.45 to 0.83), although this ratio is still greater than that of wild-type discs.

      (Crickmore and Mann, 2006)

      The increased size of the posterior compartment that is seen in the haltere discs of Ubx6.28/Ubxpbx-1 animals is partially suppressed by expression of tkvScer\UAS.cLa under the control of Scer\GAL4en-e16E.

      (de Navas et al., 2006)

      Expression of abd-A1dB5.Scer\UAS results in an almost complete rescue of the transformed haltere phenotype of Ubxabx-1 Ubxbx-3 Ubxpbx-1/UbxLDN flies. The P{GawB}UbxLDN insertion within the UbxLDN flies drives expresssion of abd-A1dB5.Scer\UAS.

      (de Navas et al., 2006)

      Ubxabx-1 Ubxbx-3 Ubxpbx-1/Ubxlac1 combination transforms the metathorax into mesothorax (including transformation of halteres into wings). This phenotype can be rescued by Scer\GAL4Bx-MS1096 induced expression abd-A. Ectopic expression of Abd-B allows partial rescue.

      (Casares et al., 1996)

      The Ubxbx-3/Ubxpbx-1 phenotype is enhanced by zae(bx).

      (Lewis, 1959)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments

      The partial transformation of haltere into wing which is seen in UbxLDN/Ubxabx-1 Ubxbx-3 Ubxpbx-1 flies is strongly suppressed by expression of either UbxScer\UAS.cCa or UbxIIa.Scer\UAS.cRa under the control of Scer\GAL4Ubx-LDN.

      The partial transformation of haltere into wing which is seen in UbxLDN/Ubxabx-1 Ubxbx-3 Ubxpbx-1 flies is partially suppressed by expression of one copy of UbxIVa.Scer\UAS.cRa under the control of Scer\GAL4Ubx-LDN and is almost completely suppressed by expression of two copies of UbxIVa.Scer\UAS.cRa under the control of Scer\GAL4Ubx-LDN.

      (de Navas et al., 2011)

      Ubxabx-1 Ubxbx-3 Ubxpbx-1/Ubxlac1 combination transforms the metathorax into mesothorax (including transformation of halteres into wings). This phenotype can be rescued by Scer\GAL4Bx-MS1096 induced expression of Ubx.

      (Casares et al., 1996)
      Images (0)
      Mutant
      Wild-type
      Stocks (9)
      Notes on Origin
      Discoverer

      Lewis, 1954.

      (Lindsley and Zimm, 1992)
      Comments
      Comments

      The phenotype of a number of combinations of Ubx mutations has been investigated to determine the role of the bxd regulatory region in the modulation of Ubx expression.

      (Castelli-Gair et al., 1992)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (5)
      Reported As
      Symbol Synonym
      Ubxpbx-1
      Ubxpbx1
      (Bischoff et al., 2009)
      pbx
      (Paul et al., 2021, Bischof et al., 2018, de Navas et al., 2011, Crickmore and Mann, 2006, Crickmore and Mann, 2006, de Navas et al., 2006, de Navas et al., 2006, Bajpai et al., 2004, Crow and Bender, 2004, Duncan and Montgomery, 2002, Lewis, 1998, Rivlin et al., 1997, Roy and VijayRaghavan, 1997, Roy et al., 1997, Casares et al., 1996, Shashidhara et al., 1995, Fernandes et al., 1994, Muralidhar and Thomas, 1993, Schneiderman et al., 1993, Cash et al., 1992, Capdevila et al., 1986, Cole and Palka, 1982, Kerridge and Morata, 1982, Minana and Garcia-Bellido, 1982, Capdevila and Garcia-Bellido, 1981, Lewis, 1959)
      pbx-1
      (O'Keefe et al., 2005)
      pbx1
      (Simoes da Silva et al., 2019, Makhijani et al., 2007, Petruk et al., 2006, Petruk et al., 2006, Guha and Kornberg, 2005, Duncan, 2002, Rivlin et al., 2001, Casares et al., 1997, Singh and Brown, 1997, Laney and Biggin, 1996, Martinez-Laborda et al., 1996, LaJeunesse and Shearn, 1995, Camprodon and Castelli-Gair, 1994, Castelli-Gair et al., 1992, Castelli-Gair et al., 1992, Lindsley and Zimm, 1992, Irvine et al., 1991, Muller and Bienz, 1991, Castelli-Gair and Garcia-Bellido, 1990, Castelli-Gair et al., 1990, Gonzalez-Gaitan et al., 1990, Little et al., 1990, Mathog, 1990, Micol et al., 1990, Botas et al., 1988, Casanova et al., 1988, Bhosekar and Babu, 1987, Lipshitz et al., 1987, Weinzierl et al., 1987, Teugels and Ghysen, 1985, Sanchez-Herrero and Morata, 1984, Bender et al., 1983)
      Name Synonyms
      Secondary FlyBase IDs
        References (66)