FlyBase Allele Report: Dmel\Sxl[F1.hs]

General Information

Symbol

Dmel\Sxl^F1.hs

Species

D. melanogaster

Name

FlyBase ID

FBal0030319

Feature type

allele

Associated gene

Dmel\Sxl

Associated Insertion(s)

Carried in Construct

P{SxlcF1}

Key Links

Transgenic product class

cDNA

(Bell et al., 1991)

wild_type

(Bell et al., 1991)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Bell et al., 1991)

wild_type

(Bell et al., 1991)

Mutagen

in vitro construct

(Bashaw and Baker, 1997, Hilfiker et al., 1994, Sakamoto et al., 1992, Bell et al., 1991, Inoue et al., 1990)

Progenitor genotype

Carried in construct

P{SxlcF1}

Cytology

Description

A female-specific Sxl cDNA is expressed under the control of an Hsp70 promoter.

(Bashaw and Baker, 1997)

Sxl⁺ cDNA under the control of an Hsp70 promoter.

(Hilfiker et al., 1994)

Female Sxl cDNA, cF1, expression is under the control of an Hsp70 promoter.

(Bell et al., 1991)

Female specific Sxl cDNA is fused to the Hsp70 promoter.

(Inoue et al., 1990)

Allele components

Component

Use(s)

Regulatory region(s)

Hsp70

Encoded product / tool

Sxl

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal | male

(Salz and Flickinger, 1996, Hilfiker et al., 1995)

Phenotype Manifest In

Detailed Description

Statement

Reference

One copy of P{SxlcF1} in wild type males, even under nonheat shock conditions, recovers only 2% of expected males.

(Salz and Flickinger, 1996)

Phenotype varies upon site of insertion. Weak class insertions have no obvious effect on male phenotype at 25^oC or 29^oC, with one or two copies of the transgene. One copy of the moderate class of insertion reduces male viability at 25^oC and becomes more severe at 29^oC. At 25^oC males are intersexes with varying degrees of feminization and are sterile, at 29^oC male escapers display this same phenotype. The strong class of insertion causes male lethality at 25^oC and 29^oC. All phenotype are in the presence of Sxl⁺.

(Bell et al., 1991)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhanced by

Statement

Reference

Sxl^F1.hs, Sxl^f1, tra¹ has abnormal sex-determination phenotype, non-enhanceable by snf^+t5.5

(Cline et al., 1999)

NOT Enhancer of

Statement

Reference

Sxl^F1.hs, tra¹, Sxl^f1, snf^+t5.5 is a non-enhancer of abnormal sex-determination phenotype of snf^+t5.5

(Cline et al., 1999)

Sxl^F1.hs, tra¹, Sxl^f1, snf^+t5.5 is a non-enhancer of lethal | male phenotype of snf^+t5.5

(Cline et al., 1999)

Suppressor of

Statement

Reference

Sxl^F1.hs is a suppressor of lethal | female | larval stage phenotype of vir^unspecified/vir^2f

(Hilfiker et al., 1995)

NOT Suppressor of

Statement

Reference

Sxl^F1.hs is a non-suppressor of lethal | female | larval stage phenotype of vir^unspecified/vir³

(Hilfiker et al., 1995)

Sxl^F1.hs is a non-suppressor of lethal | female | larval stage phenotype of vir^unspecified/vir⁴

(Hilfiker et al., 1995)

Sxl^F1.hs is a non-suppressor of lethal | female | larval stage phenotype of vir^unspecified/vir⁵

(Hilfiker et al., 1995)

Sxl^F1.hs is a non-suppressor of lethal | female | larval stage phenotype of vir^unspecified/vir⁶

(Hilfiker et al., 1995)

Sxl^F1.hs is a non-suppressor of lethal | female | larval stage phenotype of vir^unspecified/vir⁷

(Hilfiker et al., 1995)

Sxl^F1.hs is a non-suppressor of lethal | female | larval stage phenotype of vir^unspecified/vir^ts

(Hilfiker et al., 1995)

Phenotype Manifest In

Suppressed by

Statement

Reference

Sxl^F1.hs has phenotype, suppressible by snf¹

(Salz and Flickinger, 1996)

Additional Comments

Genetic Interactions

Statement

Reference

The addition of up to three copies of P{snf⁺,dhd⁺} to Sxl^f1/Y;Sxl^F1.hs, tra¹ partially feminized males does not enhance the feminisation or lethality phenotypes seen in these flies.

(Cline et al., 1999)

75% of expected males are recovered from snf¹; P{SxlcF1} males, 67% of expected males are recovered from snf^e8H; P{SxlcF1} males and 15% of expected males are recovered from snf^JA2; P{SxlcF1} males.

(Salz and Flickinger, 1996)

The presence of Sxl^F1.hs, failed to rescue female lethality in vir transheterozygotes. vir^2f/vir^- males are fertile in the presence of Sxl^F1.hs.

(Hilfiker et al., 1995)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Comments

Carried in plasmid "pB-Shsp-SxlF1cDNA", transfected into Schneider cells and used to study the role of competing splice sites in Sxl autoregulation.

(Penalva et al., 2001)

Carried in plasmid "hsp-SxlF1" and assayed in Drosophila Kc cells.

(Inoue et al., 1990)

Phenotype variability is due to quantitative variation of expression at different sites of insertion. Transgene does not have full female function.

(Bell et al., 1991)

Carried in plasmid "hsp-SxlF1cDNA".

(Sakamoto et al., 1992)

Strong class of insertion exhibits constitutive expression in the absence of heat shock.

(Hilfiker et al., 1994)

Expresses the female-specific protein in the male.

(Salz and Flickinger, 1996)

Carried in a plasmid, and cotransfected into SL-2 cells, to study the effect.

(Bashaw and Baker, 1997)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

Sxl^F1.hs

Name Synonyms

Secondary FlyBase IDs

References (13)

Report Sections

Open Close