FlyBase Allele Report: Dmel\snf[+t5.5]

General Information

Symbol

Dmel\snf^+t5.5

Species

D. melanogaster

Name

FlyBase ID

FBal0299617

Feature type

allele

Associated gene

Dmel\snf

Associated Insertion(s)

Carried in Construct

P{snf⁺,dhd⁺}

Key Links

Transgenic product class

genomic_DNA

(Larochelle et al., 1998)

wild_type

(Larochelle et al., 1998)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

genomic_DNA

(Larochelle et al., 1998)

wild_type

(Larochelle et al., 1998)

Mutagen

in vitro construct

(Larochelle et al., 1998, Hager and Cline, 1997)

Progenitor genotype

Carried in construct

P{snf⁺,dhd⁺}

Cytology

Description

Contains a 5.5kb fragment containing the entire snf and dhd genomic regions, and part of the Cdk7 gene.

(Larochelle et al., 1998)

Allele components

Component

Use(s)

Regulatory region(s)

snf

Encoded product / tool

snf

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

wild-type

(Larochelle et al., 1998)

Phenotype Manifest In

Detailed Description

Statement

Reference

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

snf^+t5.5 has lethal | male phenotype, enhanceable by Sxl^Mf1

(Cline et al., 1999)

Sxl^Mf1, snf^+t5.5 has visible phenotype, enhanceable by vir^2f

(Cline et al., 1999)

Sxl^Mf1, snf^+t5.5 has partially lethal - majority die | male phenotype, enhanceable by noi²/noi^D

(Cline et al., 1999)

NOT Enhanced by

Statement

Reference

snf^+t5.5 has abnormal sex-determination phenotype, non-enhanceable by Sxl^F1.hs/tra¹/Sxl^f1, snf^+t5.5

(Cline et al., 1999)

snf^+t5.5 has lethal | male phenotype, non-enhanceable by Sxl^F1.hs/tra¹/Sxl^f1, snf^+t5.5

(Cline et al., 1999)

Suppressed by

Statement

Reference

Sxl^M12, snf^+t5.5 has lethal phenotype, suppressible by vir^2f

(Cline et al., 1999)

NOT suppressed by

Statement

Reference

Sxl^M12, snf^+t5.5 has lethal | male phenotype, non-suppressible by snRNP-U1-70K⁶²

(Cline et al., 1999)

Sxl^M12, snf^+t5.5 has lethal | male phenotype, non-suppressible by noi²/noi^D

(Cline et al., 1999)

Sxl^M12, snf^+t5.5 has lethal phenotype, non-suppressible by snRNP-U1-70K⁶²

(Cline et al., 1999)

Sxl^M12, snf^+t5.5 has lethal phenotype, non-suppressible by noi²/noi^D

(Cline et al., 1999)

Enhancer of

Statement

Reference

snf^+t5.5 is an enhancer of partially lethal - majority die | male phenotype of Sxl^Mf1

(Cline et al., 1999)

snf^+t5.5 is an enhancer of lethal | male phenotype of Tp(1;3)sn^13a1/Sxl^f7,M1

(Cline et al., 1999)

NOT Enhancer of

Statement

Reference

snf^+t5.5 is a non-enhancer of abnormal sex-determination phenotype of Sxl^F1.hs, Sxl^f1, tra¹

(Cline et al., 1999)

snf^+t5.5 is a non-enhancer of male sterile phenotype of Sxl^M12, noi²/noi^D

(Cline et al., 1999)

snf^+t5.5 is a non-enhancer of abnormal developmental rate | male phenotype of Sxl^M12, noi²/noi^D

(Cline et al., 1999)

Sxl^F1.hs, tra¹, Sxl^f1, snf^+t5.5 is a non-enhancer of abnormal sex-determination phenotype of snf^+t5.5

(Cline et al., 1999)

Sxl^F1.hs, tra¹, Sxl^f1, snf^+t5.5 is a non-enhancer of lethal | male phenotype of snf^+t5.5

(Cline et al., 1999)

NOT Suppressor of

Statement

Reference

snf^+t5.5 is a non-suppressor of male sterile phenotype of Sxl^M12, noi²/noi^D

(Cline et al., 1999)

snf^+t5.5 is a non-suppressor of abnormal developmental rate | male phenotype of Sxl^M12, noi²/noi^D

(Cline et al., 1999)

Other

Statement

Reference

Sxl^M12, snf^+t5.5 has lethal phenotype

(Cline et al., 1999)

Sxl^Mf1, snf^+t5.5 has visible phenotype

(Cline et al., 1999)

Dp(1;1)jnR1-A, snf^+t5.5 has wild-type | male phenotype

(Cline et al., 1999)

Sxl^f7,M1, Tp(1;3)sn^13a1, snf^+t5.5 has lethal | male phenotype

(Cline et al., 1999)

Sxl^f7,M1, snf^+t5.5 has wild-type | male phenotype

(Cline et al., 1999)

Sxl^Mf1, noi²/noi^D, snf^+t5.5 has lethal | male phenotype

(Cline et al., 1999)

Phenotype Manifest In

Enhanced by

Statement

Reference

Sxl^Mf1, snf^+t5.5 has sex comb phenotype, enhanceable by noi²/noi^D

(Cline et al., 1999)

Sxl^Mf1, snf^+t5.5 has sex comb phenotype, enhanceable by snRNP-U1-70K⁶²/snRNP-U1-70K⁶²

(Cline et al., 1999)

NOT Enhanced by

Statement

Reference

Sxl^Mf1, snf^+t5.5 has sex comb phenotype, non-enhanceable by noi^+t3.6

(Cline et al., 1999)

Suppressed by

Statement

Reference

Sxl^Mf1, snf^+t5.5 has sex comb phenotype, suppressible by noi²/noi^D

(Cline et al., 1999)

NOT suppressed by

Statement

Reference

Sxl^Mf1, snf^+t5.5 has sex comb phenotype, non-suppressible by noi^+t3.6

(Cline et al., 1999)

Enhancer of

Statement

Reference

snf^+t5.5 is an enhancer of sex comb phenotype of Sxl^Mf1

(Cline et al., 1999)

snf^+t5.5 is an enhancer of eye phenotype of Sxl^Mf1

(Cline et al., 1999)

snf^+t5.5 is an enhancer of macrochaeta phenotype of Sxl^Mf1

(Cline et al., 1999)

snf^+t5.5 is an enhancer of imaginal disc phenotype of Sxl^Mf1

(Cline et al., 1999)

Other

Statement

Reference

Sxl^Mf1, snf^+t5.5 has eye phenotype

(Cline et al., 1999)

Sxl^Mf1, snf^+t5.5 has imaginal disc phenotype

(Cline et al., 1999)

Sxl^Mf1, snf^+t5.5 has sex comb phenotype

(Cline et al., 1999)

Sxl^Mf1, noi²/noi^D, snf^+t5.5 has sex comb phenotype

(Cline et al., 1999)

Sxl^Mf1, snf^+t5.5 has macrochaeta phenotype

(Cline et al., 1999)

Additional Comments

Genetic Interactions

Statement

Reference

If up to four copies of P{snf⁺,dhd⁺} are added to Sxl^f7,M1/Y, males are fully viable. If up to four copies of P{snf⁺,dhd⁺}

are added to flies with Dp(1;1)jnR1-A ( which contains a duplication

of Sxl⁺), males are fully viable. If up to 10 copies of P{snf⁺,dhd⁺}

are added to wild-type flies males are fully viable and somatically

wild-type.

However, the combination of Sxl^f7,M1/Y, Tp(1;3)sn^13a1 ( which

contains a duplication of Sxl⁺) and one copy of snf⁺ in the

transgene P{snf⁺,dhd⁺}, leads to 75% of males dying, and two

extra copies leads to complete lethality.

The addition of up to three copies of P{snf⁺,dhd⁺} to Sxl^f1/Y;Sxl^F1.hs,

tra¹ partially feminised males does not enhance the feminisation

or lethality phenotypes seen in these flies.

The addition of one copy of P{snf⁺,dhd⁺} to Sxl^Mf1/Y males

reduces the viability of males to about 50% of the expected number.

The addition of two copies reduces the survival rate to about 3%.

The survivors exhibit a wide range of imaginal discs defects, including

thin or missing bristles, as well as small and rough eyes. Also feminizing

effects are also seen in the sex combs, the majority of flies show

some loss of sex combs, and only 1% had wild-type male sex combs.

Intersexuality is predominantly of the "mosaic" type in which individual

cells are either male or female rather than intermediate.

The addition of noi^+t3.6 to Sxl^Mf1/Y males with two copies

of P{snf⁺,dhd⁺} has no significant effect on the foreleg feminisation

phenotype seen in those flies.

There is a slight increase in the foreleg feminisation phenotype seen

in Sxl^Mf1/Y males carrying two copies of P{snf⁺,dhd⁺}, when

you compare the addition of homozygous snRNP70K⁶², to heterozygous

snRNP70K⁶².

The removal of noi function (by adding noi²/noi^D) leads to

an almost complete suppression of the foreleg feminisation phenotype

seen in Sxl^Mf1/Y flies with two copies of P{snf⁺,dhd⁺}.

The addition of two copies of P{snf⁺,dhd⁺} to Sxl^Mf1/Y males

that also carry noi²/noi^D, leads to feminisation of the foreleg

and a reduction in viability.

Sxl^Mf1/Y males with a single extra copy of P{snf⁺,dhd⁺},

are considerably more masculine after the addition of Df(1)J210 (which

causes snf^J210).

The addition of P{snf⁺,dhd⁺} to snf¹, Sxl^M6/Y males,

completely removes the suppression of the Sxl^M6 lethality phenotype

seen when snf¹ is added alone.

The addition of homozygous snRNP70K⁶² does not suppress the snf^+t5.5

dose effects on Sxl^M12/Y male viability. Extra copies of P{snf⁺,dhd⁺}

do not suppress the homozygous snRNP70K⁶² phenotype.

The addition of noi²/noi^D does not suppress the snf^+t5.5

dose effects on Sxl^M12/Y male viability.

The addition of P{snf⁺,dhd⁺} has no effect on the male sterility

and developmental delay phenotypes seen in noi²/noi^D, Sxl^M12/Y

males.

(Cline et al., 1999)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

snf^+t5.5 rescues snf^J210

(Larochelle et al., 1998)

Comments

Images (0)

Mutant

Wild-type

Stocks (3)

Bloomington

4557

Df(1)JB254, P{snf⁺,dhd⁺}SL2, w^*/FM7c

58746

w¹ cm¹ Sxl^f1 ct⁶/C(1)DX, P{hs-hid}7, y¹ w¹ f¹/Y; P{snf⁺,dhd⁺}J, P{snf⁺,dhd⁺}C, P{snf⁺,dhd⁺}P

Kyoto

107796

Df(1)JB254, P{snf⁺,dhd⁺}SL2, w^*/FM7c

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

snf^+t5.5

snf^+tSa

Name Synonyms

Secondary FlyBase IDs

FBal0086332
FBal0086331

References (6)

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