FlyBase Allele Report: Dmel\Cul3[06430]

(Kaplan et al., 2010, Djagaeva and Doronkin, 2009, Arama et al., 2007, Mistry et al., 2004, Ashburner et al., 1999, Ashburner et al., 1999, Ashburner et al., 1999.10.12, Spradling et al., 1999, Roote, 1995.5.4, BDGP Project Members, 1994-1999)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal | germline clone | maternal effect | recessive

(Mistry et al., 2004)

lethal | larval stage | recessive

(Mistry et al., 2004)

lethal | recessive

(Ashburner et al., 1999, Spradling et al., 1999, Roote, 1995.5.15)

lethal - all die before end of larval stage (with Df(2L)TE35D-15)

(Mistry et al., 2004)

male fertile (with Cul3^mds1)

(Arama et al., 2007)

male fertile (with Cul3^mds2)

(Arama et al., 2007)

male fertile (with Cul3^mds3)

(Arama et al., 2007)

male fertile (with Cul3^mds4)

(Arama et al., 2007)

male fertile (with Cul3^mds5)

(Arama et al., 2007)

some die during larval stage (with Df(2L)TE35D-15)

(Mistry et al., 2004)

visible | recessive | somatic clone

(Mistry et al., 2004)

Phenotype Manifest In

campaniform sensillum | ectopic | somatic clone

(Mistry et al., 2004)

campaniform sensillum of dorsal radius | ectopic | somatic clone

(Mistry et al., 2004)

dorsal appendage | germline clone | maternal effect

(Mistry et al., 2004)

egg | germline clone | maternal effect

(Mistry et al., 2004)

external sensory organ | ectopic | somatic clone

(Mistry et al., 2004)

macrochaeta | somatic clone

(Mistry et al., 2004)

microchaeta | somatic clone

(Mistry et al., 2004)

sensory mother cell | cell autonomous | ectopic | somatic clone

(Mistry et al., 2004)

trichogen cell | ectopic | somatic clone

(Mistry et al., 2004)

wing | somatic clone

(Mistry et al., 2004)

wing disc | somatic clone

(Mistry et al., 2004)

wing hair | somatic clone

(Mistry et al., 2004)

wing vein | somatic clone

(Mistry et al., 2004)

wing vein L3 | somatic clone

(Mistry et al., 2004)

Detailed Description

Statement

Reference

Cul3⁰⁶⁴³⁰ mutant clones in sensory neurons in adult wing do not display any defects in injury-induced axon degeneration (following an axotomy, the severed axons are cleared away normally).

(Neukomm et al., 2017)

Homozygous and gft⁰⁶⁴³⁰/Df(2L)TE35D-15 animals hatch, but die during the second larval instar. Females carrying homozygous germ-line clones produce few eggs and those which are laid are about half the size of wild-type and have fused dorsal appendages. No discernible embryonic structure can be detected interior to the chorion in these eggs. Large homozygous clones in the wing show a number of defects, including defects in the overall shape of the wing and in formation and position of the wing veins. Large clones in the wing often lack trichomes in the centre of the clone. Clones that cover the L3 vein often result in it shifting posteriorly. Clones that cover wing veins are associated with loss of vein tissue. Clones in the wing also result in ectopic sensory organ formation; clones that encompass a large part of the L3 vein have ectopic campaniform sensilla, ectopic campaniform sensilla also arise between wing vein L2 and the anterior wing margin and ectopic bristles arise distally between L2 and L3. Clones in the notum are associated with significant tufting of both micro- and macrochaetae, with the tufts being made up of ectopic fully formed external sensory organs as well as individual sensory organs that contain multiple shafts within a single socket. Homozygous clones in the wing disc show a dramatic increase in the number of sensory organ precursor (SOP) cells, while SOP development outside the clone is normal.

(Mistry et al., 2004)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Cul3[+]/Cul3⁰⁶⁴³⁰ is a suppressor | partially of abnormal size phenotype of Scer\GAL4^GMR.PS, klhl10^UAS.cKa

(Aram et al., 2016)

Cul3[+]/Cul3⁰⁶⁴³⁰ is a suppressor of abnormal size | adult stage phenotype of Cul5^EP3390, Scer\GAL4^sd-SG29.1

(Ayyub et al., 2005)

gft[+]/Cul3⁰⁶⁴³⁰ is a suppressor of visible phenotype of Gαs^Q215L.UAS, Scer\GAL4^OK10

(Mistry et al., 2004)

NOT Suppressor of

Statement

Reference

Cul3⁰⁶⁴³⁰ is a non-suppressor of abnormal neuroanatomy | somatic clone | adult stage phenotype of Sarm^{ΔARM.UAS.Tag:MYC}, Scer\GAL4^VGlut-OK371

(Neukomm et al., 2017)

Phenotype Manifest In

Suppressor of

Statement

Reference

Cul3[+]/Cul3⁰⁶⁴³⁰ is a suppressor | partially of eye phenotype of Scer\GAL4^GMR.PS, klhl10^UAS.cKa

(Aram et al., 2016)

Cul3⁰⁶⁴³⁰/Cul-3[+] is a suppressor of eye phenotype of Dref^UAS.cSa

(Ida et al., 2009)

Cul3⁰⁶⁴³⁰/Cul-3[+] is a suppressor of wing blade | heat sensitive phenotype of Cul5^EP3390, Scer\GAL4^sd-SG29.1

(Ayyub et al., 2005)

gft[+]/Cul3⁰⁶⁴³⁰ is a suppressor of wing phenotype of Gαs^Q215L.UAS, Scer\GAL4^OK10

(Mistry et al., 2004)

NOT Suppressor of

Statement

Reference

Cul3⁰⁶⁴³⁰ is a non-suppressor of sensory neuron | somatic clone | adult stage phenotype of Sarm^{ΔARM.UAS.Tag:MYC}, Scer\GAL4^VGlut-OK371

(Neukomm et al., 2017)

Cul3⁰⁶⁴³⁰ is a non-suppressor of axon | somatic clone | adult stage phenotype of Sarm^{ΔARM.UAS.Tag:MYC}, Scer\GAL4^VGlut-OK371

(Neukomm et al., 2017)

Additional Comments

Genetic Interactions

Statement

Reference

The axon degeneration in sensory neurons clones in the adult wing expressing Ect4^{ΔARM.Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^VGlut-OK371 can be suppressed by combination with Cul3⁰⁶⁴³⁰.

(Neukomm et al., 2017)

Cul3⁰⁶⁴³⁰/+ partially suppresses the reduced eye phenotype of flies expressing klhl10^Scer\UAS.cKa under the control of Scer\GAL4^GMR.PS.

(Aram et al., 2016)

The wing blade defects observed in animals expressing Cul-5^EP3390 in the wing and haltere disc under the control of Scer\GAL4^sd-SG29.1 is partially rescued in a Cul-3⁰⁶⁴³⁰/+ mutant background.

(Ayyub et al., 2005)

gft⁰⁶⁴³⁰/+ moderately suppresses the phenotype caused by expression of G-sα60A^{Q215L.Scer\UAS} under the control of Scer\GAL4^OK10; wing blistering is reduced.

(Mistry et al., 2004)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Complements

Cul3^mds5

(Arama et al., 2007)

Cul3^mds4

(Arama et al., 2007)

Cul3^mds3

(Arama et al., 2007)

Cul3^mds2

(Arama et al., 2007)

Cul3^mds1

(Arama et al., 2007)

Fails to complement

Cul3^gft8

(Zhu et al., 2005)

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

23865

w^*; P{PZ}Cul3⁰⁶⁴³⁰ P{neoFRT}40A/CyO, P{hs-hid}4; P{lArB}neur^A101/TM6B, Tb¹

Notes on Origin

Discoverer

A. Spradling.

(Roote, 1995.5.4)

Comments

Precise excision of the insertion reverts the ability of gft⁰⁶⁴³⁰ to modify the phenotype caused by expression of G-sα60A^{Q215L.Scer\UAS} under the control of Scer\GAL4^OK10.

(Mistry et al., 2004)

Complements: CycE⁰⁵²⁰⁶. Complements: wek^05271a. Complements: CycE⁰⁵²⁷⁷. Complements: CycE⁰⁵²⁷⁸. Complements: CG15269⁰⁵⁴⁴¹. Complements: esg⁰⁵⁷³⁰. Complements: esg⁰⁷⁰⁸². Complements: l(2)35Bd¹⁰⁴⁰⁸. Complements: CycE¹⁰⁴²⁷.

(BDGP Project Members, 1994-1999)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (16)

Reported As

Symbol Synonym

Cul-3⁰⁶⁴³⁰

Cul3⁰⁶⁴³⁰

(Rencus-Lazar et al., 2008, Wu et al., 2005, Zhu et al., 2005, Ou et al., 2002)

cul-3⁰⁶⁴³⁰

cul-3^gft<up>06430</up>

(Arama et al., 2007)

cul3_Soma

(Kaplan et al., 2010)

cul3⁰⁶⁴³⁰

(Neukomm et al., 2017, Djagaeva and Doronkin, 2009)

cul3^P

(Djagaeva and Doronkin, 2009)

cul3^gft06430

(Aram et al., 2016, Kaplan et al., 2010)

cul3^gft<up>06430</up>

(Arama et al., 2007)

gft⁰⁶⁴³⁰

(Ida et al., 2009, Pasyukova et al., 2004)

gft⁶⁴³⁰

(Ayyub et al., 2005, Heriche et al., 2003)

gft^PZ06430

(Mistry et al., 2004, Ashburner et al., 1999)

l(2)06430

(Spradling et al., 1999)

l(2)06430⁰⁶⁴³⁰

l(2)35Cd^PZ06430

(Mistry et al., 2004)

l(2)35Cd^PZ17

(Roote, 1995.5.15)

Name Synonyms

Secondary FlyBase IDs

References (22)

Report Sections

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