FlyBase Allele Report: Dmel\Cul5[EP3390]

General Information

Symbol

Dmel\Cul5^EP3390

Species

D. melanogaster

Name

FlyBase ID

FBal0119631

Feature type

allele

Associated gene

Dmel\Cul5

Associated Insertion(s)

P{EP}Cul5^EP3390

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(Pena-Rangel et al., 2002, Tseng and Hariharan, 2002, Gene Disruption Project members, 2001-, Abdelilah-Seyfried et al., 2000)

Progenitor genotype

Associated Insertion(s)

P{EP}Cul5^EP3390

Cytology

Description

Allele components

Component

Use(s)

Inserted element

P{EP}

misexpression element

Regulatory region(s)

UAS

binary expression system - regulatory region

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

3R:29,063,851..29,063,851 [+]

(Ayyub et al., 2005, Pena-Rangel et al., 2002, Tseng and Hariharan, 2002, Gene Disruption Project members, 2001-, Abdelilah-Seyfried et al., 2000)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cell number | adult stage, with Scer\GAL4^sca-P309

(Ayyub et al., 2005)

abnormal size | adult stage, with Scer\GAL4^Dll-981

(Ayyub et al., 2005)

abnormal size | adult stage, with Scer\GAL4^GMR.PU

(Ayyub et al., 2005)

abnormal size | adult stage, with Scer\GAL4^ptc-559.1

(Ayyub et al., 2005)

abnormal size | adult stage, with Scer\GAL4^sd-SG29.1

(Ayyub et al., 2005)

increased cell number, with Scer\GAL4^sca-P309

(Ayyub et al., 2005)

increased occurrence of cell division | third instar larval stage, with Scer\GAL4^sd-SG29.1

(Ayyub et al., 2005)

lethal, with Scer\GAL4^hs.2sev

(Tseng and Hariharan, 2002)

lethal - all die before end of larval stage, with Scer\GAL4^arm.PS

(Ayyub et al., 2005)

lethal - all die before end of larval stage, with Scer\GAL4^elav.PU

(Ayyub et al., 2005)

lethal - all die before end of larval stage | conditional, with Scer\GAL4^hs.PB

(Ayyub et al., 2005)

some die during larval stage, with Scer\GAL4^arm.PS

(Ayyub et al., 2005)

some die during larval stage, with Scer\GAL4^elav.PU

(Ayyub et al., 2005)

some die during larval stage | conditional, with Scer\GAL4^hs.PB

(Ayyub et al., 2005)

visible, with Scer\GAL4^Dll-981

(Ayyub et al., 2005)

visible, with Scer\GAL4^ey.PH

(Tseng and Hariharan, 2002)

visible, with Scer\GAL4^GMR.PU

(Ayyub et al., 2005)

visible, with Scer\GAL4^ptc-559.1

(Ayyub et al., 2005)

visible, with Scer\GAL4^sca-537.4

(Abdelilah-Seyfried et al., 2000)

visible, with Scer\GAL4^sca-P309

(Ayyub et al., 2005)

visible, with Scer\GAL4^sd-SG29.1

(Ayyub et al., 2005)

visible, with Scer\GAL4^wg.PM

(Ayyub et al., 2005)

Phenotype Manifest In

adult thorax, with Scer\GAL4^ptc-559.1

(Ayyub et al., 2005)

antenna, with Scer\GAL4^ptc-559.1

(Ayyub et al., 2005)

antenna | increased number, with Scer\GAL4^wg.PM

(Ayyub et al., 2005)

arista, with Scer\GAL4^Dll-981

(Ayyub et al., 2005)

external sensory organ precursor cell IIa, with Scer\GAL4^sca-P309

(Ayyub et al., 2005)

external sensory organ precursor cell IIb | increased number, with Scer\GAL4^sca-P309

(Ayyub et al., 2005)

eye, with Scer\GAL4^ey.PH

(Tseng and Hariharan, 2002)

eye, with Scer\GAL4^GMR.PU

(Ayyub et al., 2005)

haltere | heat sensitive, with Scer\GAL4^sd-SG29.1

(Ayyub et al., 2005)

macrochaeta, with Scer\GAL4^sca-537.4

(Abdelilah-Seyfried et al., 2000)

macrochaeta, with Scer\GAL4^sca-P309

(Ayyub et al., 2005)

microchaeta, with Scer\GAL4^sca-537.4

(Abdelilah-Seyfried et al., 2000)

NMJ bouton, with Scer\GAL4^elav.PU

(Ayyub et al., 2005)

NMJ bouton, with Scer\GAL4^pros.PMG

(Ayyub et al., 2005)

sensory organ cell | increased number, with Scer\GAL4^sca-P309

(Ayyub et al., 2005)

tarsal segment, with Scer\GAL4^Dll-981

(Ayyub et al., 2005)

thecogen cell | ectopic, with Scer\GAL4^sca-537.4

(Abdelilah-Seyfried et al., 2000)

trichogen cell, with Scer\GAL4^sca-537.4

(Abdelilah-Seyfried et al., 2000)

wing, with Scer\GAL4^wg.PM

(Ayyub et al., 2005)

wing blade | heat sensitive, with Scer\GAL4^sd-SG29.1

(Ayyub et al., 2005)

wing disc | third instar larval stage, with Scer\GAL4^sd-SG29.1

(Ayyub et al., 2005)

Detailed Description

Statement

Reference

Expression of Cul-5^EP3390 in the wing and haltere disc under the control of Scer\GAL4^sd-SG29.1 at 29[o]C results in a striking reduction in the size of the wing blade and haltere, and short blistered wings form. Wing discs from third instar larvae show increased proliferation of the wing pouch region.

Expression of Cul-5^EP3390 in the distal epidermal segments under the control of Scer\GAL4^Dll-981 results in a loss of terminal structures including the arista on the antennal funiculus and the tarsi on the adult legs.

Ectopic expression of Cul-5^EP3390 under the control of Scer\GAL4^sca-P309 results in a reduced number of mechanosensory bristles (macrochaete) on the notum. 30% of sensory organ progenitors develop two PIIb-like cells and no PIIa lineage is observed. Sensory organ cluster cell number is variable in these animals, and often composed of more than 4 cells.

Flies expressing Cul-5^EP3390 under the control of Scer\GAL4^ptc-559.1 have an indented thorax, with multiple hairs from a single socket on the notum, and reduction in size of the third segment of the antenna.

Expression of Cul-5^EP3390 in the eye under the control of Scer\GAL4^GMR.PU results in adults with small eyes.

Expression of Cul-5^EP3390 under the control of Scer\GAL4^wg.PM results in flies with wing defects and duplicated antennae.

Expression of Cul-5^EP3390 in all neurons under the control of Scer\GAL4^elav.PU, or in motorneurons under the control of Scer\GAL4^pros.PMG, both results in a significant increase in the number of boutons at the neuromuscular junction of larval body wall muscles.

(Ayyub et al., 2005)

Expression of EP3390^EP3390 under the control of Scer\GAL4^ey.PH results in eyes that are slightly reduced in size. Flies expressing EP3390^EP3390 under the control of Scer\GAL4^en-e16E have wild-type wings.

(Tseng and Hariharan, 2002)

Expression of EP3390^EP3390 under the control of Scer\GAL4^sca-537.4 results in loss of external support cells of the macrochaetae and microchaetae on the notum and duplication of sheath cells (in rare cases up to 4 sheath cells are present). Shafts with a branched tip are seen.

(Abdelilah-Seyfried et al., 2000)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhanced by

Statement

Reference

Cul5^EP3390, Scer\GAL4^sca-537.4 has visible phenotype, non-enhanceable by H[+]/H^E21

(Abdelilah-Seyfried et al., 2000)

Cul5^EP3390, Scer\GAL4^sca-537.4 has visible phenotype, non-enhanceable by N[+]/N^55e11

(Abdelilah-Seyfried et al., 2000)

Suppressed by

Statement

Reference

Cul5^EP3390, Scer\GAL4^sd-SG29.1 has abnormal size | adult stage phenotype, suppressible by Cul3[+]/Cul3⁰⁶⁴³⁰

(Ayyub et al., 2005)

NOT suppressed by

Statement

Reference

Cul5^EP3390, Scer\GAL4^sca-537.4 has visible phenotype, non-suppressible by H[+]/H^E21

(Abdelilah-Seyfried et al., 2000)

Cul5^EP3390, Scer\GAL4^sca-537.4 has visible phenotype, non-suppressible by N[+]/N^55e11

(Abdelilah-Seyfried et al., 2000)

Enhancer of

Statement

Reference

Scer\GAL4^pnr-MD237/Cul5^EP3390 is an enhancer of visible phenotype of pnr^MD237

(Pena-Rangel et al., 2002)

Phenotype Manifest In

Suppressed by

Statement

Reference

Cul5^EP3390, Scer\GAL4^sd-SG29.1 has wing blade | heat sensitive phenotype, suppressible by wg^l-12.UAS, Scer\GAL4^sd-SG29.1

(Ayyub et al., 2005)

Cul5^EP3390, Scer\GAL4^sd-SG29.1 has wing blade | heat sensitive phenotype, suppressible by Cul3⁰⁶⁴³⁰/Cul-3[+]

(Ayyub et al., 2005)

Enhancer of

Statement

Reference

Scer\GAL4^pnr-MD237/Cul5^EP3390 is an enhancer of chaeta phenotype of pnr^MD237

(Pena-Rangel et al., 2002)

NOT Enhancer of

Statement

Reference

Cul5^EP3390/Scer\GAL4^sca-537.4 is a non-enhancer of phenotype of N^55e11

(Abdelilah-Seyfried et al., 2000)

Cul5^EP3390/Scer\GAL4^sca-537.4 is a non-enhancer of phenotype of H^E21

(Abdelilah-Seyfried et al., 2000)

NOT Suppressor of

Statement

Reference

Cul5^EP3390/Scer\GAL4^sca-537.4 is a non-suppressor of phenotype of H^E21

(Abdelilah-Seyfried et al., 2000)

Other

Statement

Reference

Cul5^EP3390, Scer\GAL4^pnr-MD237, pnr^MD237/pnr[+] has scutellar bristle | increased number phenotype

(Pena-Rangel et al., 2002)

Cul5^EP3390, Scer\GAL4^pnr-MD237, pnr^MD237/pnr[+] has dorsocentral bristle | increased number phenotype

(Pena-Rangel et al., 2002)

Cul5^EP3390, Scer\GAL4^pnr-MD237, pnr^MD237 has scutellar bristle | increased number phenotype

(Pena-Rangel et al., 2002)

Cul5^EP3390, Scer\GAL4^pnr-MD237, pnr^MD237 has dorsocentral bristle | increased number phenotype

(Pena-Rangel et al., 2002)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of wg^{l-12.Scer\UAS} in animals expressing Cul-5^EP3390 in the wing and haltere disc under the control of Scer\GAL4^sd-SG29.1 partially rescues the wing-blade defects observed at 29[o]C.

The wing blade defects observed in animals expressing Cul-5^EP3390 in the wing and haltere disc under the control of Scer\GAL4^sd-SG29.1 is partially rescued in a Cul-3⁰⁶⁴³⁰/+ mutant background.

(Ayyub et al., 2005)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Selected as: a P{EP} insertion line that modifies the pnr^MD237/+ phenotype when expressed using Scer\GAL4^pnr-MD237.

(Pena-Rangel et al., 2002)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

Cul-5^EP3390

Cul5^EP3390

EP(3)3390

(Abdelilah-Seyfried et al., 2000)

EP3390^EP3390

Name Synonyms

Secondary FlyBase IDs

References (5)

Report Sections

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