FlyBase Allele Report: Dmel\pnt[1277]

General Information

Symbol

Dmel\pnt¹²⁷⁷

Species

D. melanogaster

Name

FlyBase ID

FBal0035442

Feature type

allele

Associated gene

Dmel\pnt

Associated Insertion(s)

P{lacW}pnt¹²⁷⁷

Carried in Construct

Also Known As

pnt-lacZ, pntP2-lacZ

Key Links

Genomic Maps

JBrowse

Allele class

hypomorphic allele - genetic evidence

Mutagen

Delta2-3 transposase

Nature of the Allele

Allele class

hypomorphic allele - genetic evidence

(Isaksson et al., 1997, Albagli et al., 1996)

Mutagen

Delta2-3 transposase

(Scholz et al., 1993)

Progenitor genotype

Associated Insertion(s)

P{lacW}pnt¹²⁷⁷

Cytology

Description

Insertion of a P-element.

(Brunner et al., 1994)

12kb P-element transposon insertion into the middle of exon 1 at position +365 of the P2 pnt transcript.

(Scholz et al., 1993)

Allele components

Component

Use(s)

Inserted element

P{lacW}

enhancer trap

Encoded product / tool

lacZ

reporter enzyme

Tagged with

Tag:NLS(P)

nuclear protein localization tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

3R:23,345,398..23,346,162 [-]

(Charroux et al., 2006, Frankfort and Mardon, 2004, Brunner et al., 1994, Scholz et al., 1993)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cell polarity (with pnt^Δ88)

(Brown and Freeman, 2003)

abnormal planar polarity (with pnt¹²³⁰)

(Weber et al., 2008)

abnormal planar polarity (with pnt^Δ88)

(Weber et al., 2008)

viable

(Isaksson et al., 1997, Albagli et al., 1996)

visible (with pnt^Δ88)

(Rendina et al., 2010)

Phenotype Manifest In

eye (with pnt^Δ88)

(Tchankouo-Nguetcheu et al., 2014, Rendina et al., 2010)

eye photoreceptor cell

(Albagli et al., 1996)

eye photoreceptor cell (with pnt^Δ88)

(Rendina et al., 2010)

ommatidium (with pnt¹²³⁰)

(Weber et al., 2008)

ommatidium (with pnt^Δ88)

(Fetting et al., 2009, Weber et al., 2008, Brown and Freeman, 2003, Bai et al., 2001)

photoreceptor (with pnt^Δ88)

(Brown and Freeman, 2003)

photoreceptor cell R7

(Albagli et al., 1996)

photoreceptor cell R7 (with pnt^Δ88)

(Bai et al., 2001)

Detailed Description

Statement

Reference

pnt^Δ88/pnt¹²⁷⁷ flies have weakly rough eyes.

(Tchankouo-Nguetcheu et al., 2014)

pnt^Δ88/pnt¹²⁷⁷ flies have rough eyes and show a loss of photoreceptor cells.

(Rendina et al., 2010)

Many pnt^Δ88/pnt¹²⁷⁷ ommatidia over- or under-rotate; the variance (s.d.) in degree of rotation is greater than in wild type in adult eyes.

(Fetting et al., 2009)

pnt¹²⁷⁷/pnt¹²³⁰ eye discs show polarity defects (10% of ommatidial clusters are inverted and 9% are symmetrical).

pnt¹²⁷⁷/pnt^Δ88 eye discs show polarity defects (6.7% of ommatidial clusters are inverted and 6% are symmetrical).

(Weber et al., 2008)

pnt¹²⁷⁷/pnt^Δ88 flies display a weak rough eye phenotype but pnt¹²⁷⁷/pnt⁰⁷⁸²⁵ flies have normal eyes.

(Charroux et al., 2006)

In pnt¹²⁷⁷/pnt^Δ88 mutant adults defects are seen in the eye. Under-recruitment of phtoreceptors is common, though in those ommatidia that are correctly specified, mis-rotations are seen.

(Brown and Freeman, 2003)

pnt¹²⁷⁷/pnt^Δ88 show reduced number of R7 photoreceptors.

(Bai et al., 2001)

90% of the ommatidia in pnt^Δ88/pnt¹²⁷⁷ flies lack the R7 photoreceptor and/or some outer photoreceptor cells.

(Albagli et al., 1996)

Viable. Heterozygotes with pnt^Δ88 lack one to three photoreceptor cells in most ommatidia. The R7 precursor can be rescued by pnt P2 transcript expressed by sev enhancers but the mutant phenotype is enhanced by pnt^{T151A.Scer\UAS} expressed by sev enhancers.

(Brunner et al., 1994)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

pnt¹²⁷⁷/pnt^Δ88 has visible phenotype, enhanceable by Scer\GAL4^elav.PU/Bap170^C1.UAS

(Rendina et al., 2010)

Suppressed by

Statement

Reference

pnt¹²⁷⁷/pnt^Δ88 has visible phenotype, suppressible by Bap170^hfl1/Bap170[+]

(Rendina et al., 2010)

Suppressor of

Statement

Reference

pnt¹²⁷⁷ is a suppressor of visible phenotype of faf^BX4

(Isaksson et al., 1997)

Phenotype Manifest In

Enhanced by

Statement

Reference

pnt¹²⁷⁷/pnt^Δ88 has eye photoreceptor cell phenotype, enhanceable by Scer\GAL4^elav.PU/Bap170^C1.UAS

(Rendina et al., 2010)

pnt¹²⁷⁷/pnt^Δ88 has eye phenotype, enhanceable by Scer\GAL4^elav.PU/Bap170^C1.UAS

(Rendina et al., 2010)

pnt¹²⁷⁷/pnt^Δ88 has ommatidium phenotype, enhanceable by ed[+]/ed^1X5

(Fetting et al., 2009)

NOT Enhanced by

Statement

Reference

pnt¹²⁷⁷/pnt^Δ88 has eye phenotype, non-enhanceable by Ptp61F[+]/Ptp61F^4-10

(Tchankouo-Nguetcheu et al., 2014)

pnt¹²⁷⁷/pnt^Δ88 has eye phenotype, non-enhanceable by Ptp61F^UAS.cBa/Scer\GAL4^GMR.PU

(Tchankouo-Nguetcheu et al., 2014)

Suppressed by

Statement

Reference

pnt¹²⁷⁷/pnt^Δ88 has eye photoreceptor cell phenotype, suppressible by Bap170^hfl1/Bap170[+]

(Rendina et al., 2010)

pnt¹²⁷⁷/pnt^Δ88 has ommatidium phenotype, suppressible by fred[+]/fred^H24

(Fetting et al., 2009)

pnt¹²⁷⁷/pnt^Δ88 has photoreceptor cell R7 phenotype, suppressible by ed^slH8/ed^1X5

(Bai et al., 2001)

pnt¹²⁷⁷/pnt^Δ88 has ommatidium phenotype, suppressible by ed^slH8/ed^1X5

(Bai et al., 2001)

pnt¹²⁷⁷ has eye photoreceptor cell phenotype, suppressible by Scer\GAL4^hs.2sev/Ggal\ets2^UAS.cAa

(Albagli et al., 1996)

pnt¹²⁷⁷ has eye photoreceptor cell phenotype, suppressible by ALV\v-ets^UAS.cAa/Scer\GAL4^hs.2sev

(Albagli et al., 1996)

NOT suppressed by

Statement

Reference

pnt¹²⁷⁷/pnt^Δ88 has eye phenotype, non-suppressible by Ptp61F[+]/Ptp61F^4-10

(Tchankouo-Nguetcheu et al., 2014)

pnt¹²⁷⁷/pnt^Δ88 has eye phenotype, non-suppressible by Ptp61F^UAS.cBa/Scer\GAL4^GMR.PU

(Tchankouo-Nguetcheu et al., 2014)

Enhancer of

Statement

Reference

pnt¹²⁷⁷/pnt^Δ88 is an enhancer of interommatidial bristle phenotype of Gug^UAS.cCa, Scer\GAL4^GMR.PF

(Charroux et al., 2006)

pnt¹²⁷⁷/pnt⁰⁷⁸²⁵ is an enhancer of ommatidium phenotype of Gug^UAS.cCa, Scer\GAL4^GMR.PF

(Charroux et al., 2006)

Suppressor of

Statement

Reference

pnt¹²⁷⁷ is a suppressor of photoreceptor cell R2 | ectopic phenotype of faf^BX4

(Isaksson et al., 1997)

pnt¹²⁷⁷ is a suppressor of photoreceptor cell R3 | ectopic phenotype of faf^BX4

(Isaksson et al., 1997)

pnt¹²⁷⁷ is a suppressor of photoreceptor cell R4 | ectopic phenotype of faf^BX4

(Isaksson et al., 1997)

pnt¹²⁷⁷ is a suppressor of photoreceptor cell R5 | ectopic phenotype of faf^BX4

(Isaksson et al., 1997)

pnt¹²⁷⁷ is a suppressor of photoreceptor cell R6 | ectopic phenotype of faf^BX4

(Isaksson et al., 1997)

pnt¹²⁷⁷ is a suppressor of photoreceptor cell R1 | ectopic phenotype of faf^BX4

(Isaksson et al., 1997)

pnt¹²⁷⁷ is a suppressor of phenotype of Jra^Asp.hs.sev

(Treier et al., 1995)

Additional Comments

Genetic Interactions

Statement

Reference

Bap170^hfl1/+ suppresses the loss of photoreceptor cells seen in pnt^Δ88/pnt¹²⁷⁷ flies.

Expression of Bap170^C1.Scer\UAS under the control of Scer\GAL4^elav.PU enhances the rough eye phenotype and loss of photoreceptor cells seen in pnt^Δ88/pnt¹²⁷⁷ flies.

(Rendina et al., 2010)

ed^1X5 dominantly enhances the phenotype of mis-rotation of ommatidia that is seen in pnt^Δ88/pnt¹²⁷⁷ animals.

fred^H24 dominantly suppresses the phenotype of mis-rotation of ommatidia that is seen in pnt^Δ88/pnt¹²⁷⁷ animals.

(Fetting et al., 2009)

The rough eye phenotype of flies expressing Gug^Scer\UAS.cCa under the control of Scer\GAL4^GMR.PF is enhanced in both a pnt¹²⁷⁷/pnt^Δ88 and a pnt¹²⁷⁷/pnt⁰⁷⁸²⁵ background.

(Charroux et al., 2006)

The extra outer photoreceptor cell phenotype seen in faf^BX4 homozygotes is suppressed by pnt¹²⁷⁷, with the average number of outer photoreceptor cells per ommatidium being reduced to 6.2.

(Isaksson et al., 1997)

pnt^Δ88/pnt¹²⁷⁷ or pnt¹²³⁰/pnt¹²⁷⁷ completely suppresses the ectopic R7 rough eye phenotype of Jra^Asp.hs.sev.

(Treier et al., 1995)

Xenogenetic Interactions

Statement

Reference

The eye phenotype of pnt^Δ88/pnt¹²⁷⁷ flies is partially rescued when Ggal\ets2^Scer\UAS.cAa or ALV\v-ets^Scer\UAS.cAa is expressed using Scer\GAL4^hs.2sev.

(Albagli et al., 1996)

Complementation and Rescue Data

Partially rescued by

pnt¹²⁷⁷/pnt^Δ88 is partially rescued by Scer\GAL4^hs.2sev/pnt^P1.UAS

(Albagli et al., 1996)

Comments

The eye phenotype of pnt^Δ88/pnt¹²⁷⁷ flies is partially rescued when pnt^P1.Scer\UAS is expressed using Scer\GAL4^hs.2sev at 17^oC.

(Albagli et al., 1996)

The R7 precursor can be rescued by pnt P2 transcript expressed by sev enhancers but the mutant phenotype is enhanced by pnt^{T151A.Scer\UAS} expressed by sev enhancers.

(Brunner et al., 1994)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

837

w¹¹¹⁸; P{lacW}pnt¹²⁷⁷

Notes on Origin

Discoverer

Beermann.

(Scholz et al., 1993)

Comments

Allelic series of pnt allele that affect the P2 transcript: pnt¹²⁷⁷ < pnt¹²⁰² < pnt¹²⁰⁸ < pnt¹²³⁰ <= pnt^T9 < pnt^T5.

(Scholz et al., 1993)

Weak P2-specific pnt mutant.

(Brunner et al., 1994)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

pnt¹²⁷⁷

(Tchankouo-Nguetcheu et al., 2014, Rendina et al., 2010, Fetting et al., 2009, Weber et al., 2008)

Name Synonyms

Secondary FlyBase IDs

References (14)

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