FlyBase Allele Report: Dmel\pnt[P2.UAS]

General Information

Symbol

Dmel\pnt^P2.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0035444

Feature type

allele

Associated gene

Dmel\pnt

Associated Insertion(s)

Carried in Construct

P{UAS-pnt.P2}

Also Known As

UAS-pntP2, UAS-pnt.P2, uas-pointedP2

Key Links

Transgenic product class

cDNA

(Brunner et al., 1994)

wild_type

(Brunner et al., 1994)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Brunner et al., 1994)

wild_type

(Brunner et al., 1994)

Mutagen

in vitro construct

(Brunner et al., 1994, Klaes et al., 1994)

Progenitor genotype

Carried in construct

P{UAS-pnt.P2}

Cytology

Description

UASt regulatory sequences drive expression of the pnt P2 cDNA.

(Brunner et al., 1994)

UASt regulatory sequences drive expression of the pnt P2 isoform.

(Klaes et al., 1994)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

pnt

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal planar polarity, with Scer\GAL4^hs.2sev

(Weber et al., 2008)

increased occurrence of cell division | adult stage, with Scer\GAL4^Delta.PU

(Jin et al., 2015)

increased occurrence of cell division | adult stage, with Scer\GAL4^NP5130

(Jin et al., 2015)

viable, with Scer\GAL4^nanos.PG

(Tracey et al., 2000)

Phenotype Manifest In

adult midgut, with Scer\GAL4^Delta.PU

(Jin et al., 2015)

adult midgut, with Scer\GAL4^NP5130

(Jin et al., 2015)

dorsal appendage, with Scer\GAL4^c532

(Deng and Bownes, 1997)

intestinal stem cell | adult stage, with Scer\GAL4^Delta.PU

(Jin et al., 2015)

intestinal stem cell | adult stage, with Scer\GAL4^NP5130

(Jin et al., 2015)

ommatidium, with Scer\GAL4^hs.2sev

(Weber et al., 2008)

photoreceptor cell R7, with Scer\GAL4^hs.2sev

(Brunner et al., 1994)

Detailed Description

Statement

Reference

The expression of pnt^P1.Scer\UAS under the control of Scer\GAL4^prd.PU leads to no significant changes in the number of oenocytes in Prd-positive thoracic or abdominal segments during stage 15 of embryogenesis, as compared to controls.

(Wang et al., 2017)

Expression of pnt^P2.Scer\UAS under the control of either Scer\GAL4^esg-NP5130 or Scer\GAL4^Dl.PU (using tub-Gal80[ts] to limit the time of the transgene expression) results in intestinal stem cell overproliferation in the adult midgut.

(Jin et al., 2015)

Expression of pnt^P2.Scer\UAS under the control of Scer\GAL4^hs.2sev results in 70% of ommatidia being symmetrical.

(Weber et al., 2008)

Overexpression of pnt^P2.Scer\UAS, under the control of Scer\GAL4^en-e16E, induces vein loss in 0% in vein L4 and 89% in vein L5.

(Marenda et al., 2006)

Expression of pnt^P2.Scer\UAS under the control of Scer\GAL4^slbo.2.6 does not affect border cell motility.

(Schober et al., 2005)

Expression of pnt^P2.Scer\UAS driven by Scer\GAL4^twi.PG has no effect on cardioblast development.

(Alvarez et al., 2003)

Expression of pnt^P2.Scer\UAS under the control of Scer\GAL4^ey.PB does not result in homeotic transformation of the eye to antenna.

(Kumar and Moses, 2001)

Expression of pnt^P2.Scer\UAS under the control of Scer\GAL4^dpp.blk1 does not result in ectopic furrow formation in the eye disc.

(Kumar and Moses, 2001)

Expression of pnt^P2.Scer\UAS under the control of Scer\GAL4^nos.PG has no discernible effect on the viability or phenotype of embryos.

(Tracey et al., 2000)

Scer\GAL4^c532-mediated expression causes thinner and shorter dorsal appendages.

(Deng and Bownes, 1997)

Scer\GAL4^55B and Scer\GAL4^c324a mediated expression produces female progeny that lay wild type eggs that develop normally.

(Morimoto et al., 1996)

When expression is driven by Scer\GAL4^hs.2sev, in an otherwise pnt^Δ88/pnt¹²⁷⁷ background, an R7 photoreceptor is present in almost every ommatidium.

(Brunner et al., 1994)

Ectopic expression of the P2 form of pnt driven by either Scer\GAL4^rho.PL or Scer\GAL4^sca-537.4 has no phenotypic effect.

(Klaes et al., 1994)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Scer\GAL4^hs.2sev, pnt^P2.UAS has abnormal planar polarity phenotype, suppressible | partially by Jra^UAS.cEa, Scer\GAL4^hs.2sev

(Weber et al., 2008)

Scer\GAL4^hs.2sev, pnt^P2.UAS has abnormal planar polarity phenotype, suppressible | partially by aop^EP598/Scer\GAL4^hs.2sev

(Weber et al., 2008)

NOT suppressed by

Statement

Reference

Scer\GAL4^hs.2sev, pnt^P2.UAS has abnormal planar polarity phenotype, non-suppressible by Scer\GAL4^hs.2sev/kay^UAS.cEa

(Weber et al., 2008)

NOT Enhancer of

Statement

Reference

pnt^P2.UAS, Scer\GAL4^GMR.PF is a non-enhancer of visible phenotype of Asam\aopP^UAS.Tag:MYC, Scer\GAL4^GMR.PF

(Jones et al., 2012)

Suppressor of

Statement

Reference

Scer\GAL4^ap-md544/pnt^P2.UAS is a suppressor of visible phenotype of Bx¹

(Bejarano et al., 2008)

NOT Suppressor of

Statement

Reference

pnt^P2.UAS, Scer\GAL4^GMR.PF is a non-suppressor of visible phenotype of Asam\aopP^UAS.Tag:MYC, Scer\GAL4^GMR.PF

(Jones et al., 2012)

Phenotype Manifest In

Suppressed by

Statement

Reference

Scer\GAL4^hs.2sev, pnt^P2.UAS has ommatidium phenotype, suppressible | partially by Jra^UAS.cEa, Scer\GAL4^hs.2sev

(Weber et al., 2008)

Scer\GAL4^hs.2sev, pnt^P2.UAS has ommatidium phenotype, suppressible | partially by aop^EP598/Scer\GAL4^hs.2sev

(Weber et al., 2008)

NOT suppressed by

Statement

Reference

Scer\GAL4^hs.2sev, pnt^P2.UAS has ommatidium phenotype, non-suppressible by Scer\GAL4^hs.2sev/kay^UAS.cEa

(Weber et al., 2008)

NOT Enhancer of

Statement

Reference

pnt^P2.UAS, Scer\GAL4^GMR.PF is a non-enhancer of eye phenotype of Asam\aopP^UAS.Tag:MYC, Scer\GAL4^GMR.PF

(Jones et al., 2012)

Suppressor of

Statement

Reference

Scer\GAL4^ap-md544/pnt^P2.UAS is a suppressor of wing margin phenotype of Bx¹

(Bejarano et al., 2008)

NOT Suppressor of

Statement

Reference

pnt^P2.UAS, Scer\GAL4^GMR.PF is a non-suppressor of eye phenotype of Asam\aopP^UAS.Tag:MYC, Scer\GAL4^GMR.PF

(Jones et al., 2012)

Other

Statement

Reference

Raf^UAS.F179, Scer\GAL4^GMR.PY, pnt^P2.UAS has ommatidial precursor cluster phenotype

(Yamada et al., 2003)

Raf^UAS.F179, Scer\GAL4^GMR.PY, pnt^P2.UAS has photoreceptor cell R4 phenotype

(Yamada et al., 2003)

Raf^UAS.F179, Scer\GAL4^GMR.PY, pnt^P2.UAS has photoreceptor cell R3 phenotype

(Yamada et al., 2003)

Raf^UAS.F179, Scer\GAL4^GMR.PY, pnt^P2.UAS has photoreceptor cell R1 phenotype

(Yamada et al., 2003)

Raf^UAS.F179, Scer\GAL4^GMR.PY, pnt^P2.UAS has photoreceptor cell R6 phenotype

(Yamada et al., 2003)

Raf^UAS.F179, Scer\GAL4^en-e16E, pnt^P2.UAS has abdominal lateral pentascolopidial chordotonal organ lch5 phenotype

(Yamada et al., 2003)

Additional Comments

Genetic Interactions

Statement

Reference

The increase in the adult midgut intestinal stem cell proliferation characteristic for flies expressing pnt^P2.Scer\UAS under the control of Scer\GAL4^esg-NP5130 (using tub-Gal80[ts] to limit the time of the transgene expression) is not suppressed by co-expression of cic^{ΔC2.Scer\UAS.T:Ivir\HA1}.

(Jin et al., 2015)

Co-expression of kay^Scer\UAS.cEa has no effect on the symmetrical ommatidia phenotype caused by expression of pnt^P2.Scer\UAS under the control of Scer\GAL4^hs.2sev.

Co-expression of either Jra^Scer\UAS.cEa or aop^EP598 partially suppresses the symmetrical ommatidia phenotype caused by expression of pnt^P2.Scer\UAS under the control of Scer\GAL4^hs.2sev.

(Weber et al., 2008)

Co-expression of pnt^P2.Scer\UAS with msk^Scer\UAS.cLa, both under the control of Scer\GAL4^en-e16E, further enhances the wing vein loss phenotype observed in pnt^P2.Scer\UAS single mutants, with vein loss to 97% in vein L4 and 100% in vein L5.

(Marenda et al., 2006)

When phl^{Scer\UAS.F179} and pnt^P2.Scer\UAS are coexpressed in the embryo under the control of Scer\GAL4^en-e16E, the number of scolopidia in the lch5 organs is reduced from the normal number of 5 to 3 or 4 in 71% of hemisegments. When phl^{Scer\UAS.F179} and pnt^P2.Scer\UAS are coexpressed under the control of Scer\GAL4^GMR.PY in cells posterior to the morphogenetic furrow, most ommatidial clusters in the early stages of ommatidial assembly contain fewer than the normal number of neurons, with the specification of R3, R4, R1 and R6 being severely disrupted.

(Yamada et al., 2003)

Xenogenetic Interactions

Statement

Reference

Co-expression of pnt^P2.Scer\UAS with Zzzz\aopP^{Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^GMR.PF does not alter the eye morphology of Zzzz\aopP^{Scer\UAS.T:Hsap\MYC}-expressing eyes.

(Jones et al., 2012)

Complementation and Rescue Data

Rescues

Scer\GAL4^twi.PG/pnt^P2.UAS rescues pnt^S012309

(Alvarez et al., 2003)

pnt^P2.UAS/Scer\GAL4^sli.PS rescues pnt^Δ88

(Scholz et al., 1997)