FlyBase Allele Report: Dmel\mew[498]

General Information

Symbol

Dmel\mew⁴⁹⁸

Species

D. melanogaster

Name

FlyBase ID

FBal0039652

Feature type

allele

Associated gene

Dmel\mew

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

loss of function allele

amorphic allele - genetic evidence

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

amorphic allele - genetic evidence

(Brower et al., 1995)

loss of function allele

(Roote and Zusman, 1996)

Mutagen

ethyl methanesulfonate

(Brower et al., 1995)

Progenitor genotype

Cytology

Description

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal | recessive

(Hoang and Chiba, 1998, Brower et al., 1995)

Phenotype Manifest In

Detailed Description

Statement

Reference

Stage 14 oocytes carrying mew⁴⁹⁸ somatic clones are much more rounded than wild-type.

(Bateman et al., 2001)

Homozygous embryos have an increased rate of axon errors in the central and peripheral nervous systems compared to wild-type.

(Hoang and Chiba, 1998)

mew⁴⁹⁸ somatic wing clones cause blisters. Scer\GAL4^how-24B-mediated expression of mew^Scer\UAS.cWa or mew^Δ.Scer\UAS rescues the mutant phenotype. Temperature shift experiments for higher levels of Scer\GAL4 expression suggest mew expression is required late in wing development. Clones in the eye disrupt photoreceptor organisation. Scer\GAL4^hs.PB-mediated expression of mew^Scer\UAS.cWa or mew^Δ.Scer\UAS rescues the disorganised phenotype.

(Li et al., 1998)

In 30 and 36 hour after pupariation (AP) dorsal clones are associated with pronounced matrix-filled blisters.

(Brabant et al., 1996)

Mitotic clones in the eye exhibit photoreceptor disorganisation and holes resulting in spaces between and within ommatidial units. Disruption is mild and defects are often restricted to the basal surface of the retina.

(Roote and Zusman, 1996)

Midgut does not elongate into the tubular structure but there are no obvious defects in somatic muscle morphogenesis. Dorsal herniation is not observed in homozygotes or double mutant combinations with if^k27e and if^B4. Large dorsal wing clones cause wing blisters, small clones of approximately 5 cells have no phenotype and clones of 100 cells show a mild phenotype such as wrinkles confined to one end of the clone. Large dorsal wing clones cause wing blisters, small clones of approximately 5 cells have no phenotype and clones of 100 cells show a mild phenotype such as wrinkles confined to one end of the clone. Clones in the eye exhibit photoreceptor disorganisation.

(Brower et al., 1995)

Clonal analysis reveals that lack of mew has no discernible effect on germ cell development. Mutant embryos show a distinct and reproducible separation between the ectodermal and mesodermal tissue layers of the germband, though the separation is not complete. Midgut primordia meet and initiate fusion at least 1 to 2 hours after wild type embryos would. Visceral mesoderm appears normal. Midgut constrictions partially form.

(Roote and Zusman, 1995)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

mew[+]/mew⁴⁹⁸ is a suppressor of visible | dominant phenotype of sog^EP7

(Araujo et al., 2003)

mew[+]/mew⁴⁹⁸ is a suppressor of visible | dominant phenotype of sog^EP11

(Araujo et al., 2003)

Phenotype Manifest In

Suppressed by

Statement

Reference

if^k27e, mew⁴⁹⁸ has midgut primordium phenotype, suppressible by if^Δ.C.UAS/Scer\GAL4^how-24B

(Li et al., 1998)

if^k27e, mew⁴⁹⁸ has midgut primordium phenotype, suppressible by if^Δ.m8.UAS/Scer\GAL4^how-24B

(Li et al., 1998)

mew⁴⁹⁸ has photoreceptor neuron phenotype, suppressible by if^C.UAS/Scer\GAL4^hs.PB

(Li et al., 1998)

mew⁴⁹⁸ has photoreceptor neuron phenotype, suppressible by if^Δ.C.UAS/Scer\GAL4^hs.PB

(Li et al., 1998)

mew⁴⁹⁸ has photoreceptor neuron phenotype, suppressible by if^m8.UAS/Scer\GAL4^hs.PB

(Li et al., 1998)

mew⁴⁹⁸ has photoreceptor neuron phenotype, suppressible by if^Δ.m8.UAS/Scer\GAL4^hs.PB

(Li et al., 1998)

mew⁴⁹⁸ has retina | somatic clone phenotype, suppressible by if^C.UAS/Scer\GAL4^hs.PB

(Roote and Zusman, 1996)

mew⁴⁹⁸ has retina | somatic clone phenotype, suppressible by if^m8.UAS/Scer\GAL4^hs.PB

(Roote and Zusman, 1996)

NOT suppressed by

Statement

Reference

mew⁴⁹⁸ has wing | somatic clone phenotype, non-suppressible by Scer\GAL4⁶⁸⁴/if^C.UAS

(Brabant et al., 1996)

Suppressor of

Statement

Reference

mew[+]/mew⁴⁹⁸ is a suppressor of wing vein phenotype of sog^EP11

(Araujo et al., 2003)

mew[+]/mew⁴⁹⁸ is a suppressor of wing vein phenotype of sog^EP7

(Araujo et al., 2003)

Other

Statement

Reference

Scer\GAL4^how-24B, if^Δ.C.UAS, mew⁴⁹⁸ has midgut primordium phenotype

(Li et al., 1998)

Scer\GAL4^how-24B, if^Δ.m8.UAS, mew⁴⁹⁸ has midgut primordium phenotype

(Li et al., 1998)

if^k27e, mew⁴⁹⁸ has midgut primordium phenotype

(Li et al., 1998)

if^k27e, mew⁴⁹⁸ has somatic muscle cell phenotype

(Roote and Zusman, 1995)

if^B4, mew⁴⁹⁸ has somatic muscle cell phenotype

(Roote and Zusman, 1995)

Additional Comments

Genetic Interactions

Statement

Reference

mew⁴⁹⁸ if^k27e double mutants have no midgut constrictions. Scer\GAL4^how-24B-mediated expression of if^{Δ.C.Scer\UAS} or if^{Δ.m8.Scer\UAS} rescues initiation of midgut constrictions. Scer\GAL4^hs.PB-mediated expression of if^C.Scer\UAS, if^m8.Scer\UAS, if^{Δ.C.Scer\UAS} or if^{Δ.m8.Scer\UAS} also allow some rescue of the eye phenotype of mew⁴⁹⁸ clones.

(Li et al., 1998)

Scer\GAL4⁶⁸⁴ induced expression of if^C.Scer\UAS fails to rescue the blistering phenotype.

(Brabant et al., 1996)

Expression of if^C.Scer\UAS and if^m8.Scer\UAS also rescues the mutant phenotype in most of the clones with similar efficiency to mew^Scer\UAS.cWa.

(Roote and Zusman, 1996)

Double mutants for mew and if have a midgut phenotype as severe as that of mys mutants. Somatic muscle appears normal. Dorsal hole and U-shaped cuticle of mys mutants are not observed in mew or if mutants. Double mutants between mew⁴⁹⁸, mew⁰²³ or mew^M6 and if^k27e or if^B4 do not show the twisted germband, amnioserosal detachment, dorsal movement of germband, dorsal hole and U-shaped embryo phenotypes of mys mutants, but do show early muscle detachments, as seen for mys mutants.

(Roote and Zusman, 1995)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

mew⁴⁹⁸ is rescued by mew^Δ.UAS/Scer\GAL4^hs.PB

(Li et al., 1998)

mew⁴⁹⁸ is rescued by Scer\GAL4^how-24B/mew^Δ.UAS

(Li et al., 1998)

mew⁴⁹⁸ is rescued by Scer\GAL4^how-24B/mew^UAS.cWa

(Li et al., 1998)

mew⁴⁹⁸ is rescued by mew^UAS.cWa/Scer\GAL4^hs.PB

(Li et al., 1998)

mew⁴⁹⁸ is rescued by Scer\GAL4⁶⁸⁴/mew^UAS.cWa

(Brabant et al., 1996)

Comments

Scer\GAL4⁶⁸⁴ induced expression of mew^Scer\UAS.cWa rescues dorsal clones completely.

(Brabant et al., 1996)

Scer\GAL4^hs.PB mediated expression of mew^Scer\UAS.cWa in the clones completely rescues the disorganised photoreceptor phenotype in 85% of the clones and partially in the remaining clones.

(Roote and Zusman, 1996)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

mew⁴⁹⁸

Name Synonyms

Secondary FlyBase IDs

References (8)

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