FlyBase Allele Report: Dmel\if[m8.UAS]

General Information

Symbol

Dmel\if^m8.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0051512

Feature type

allele

Associated gene

Dmel\if

Associated Insertion(s)

Carried in Construct

P{UAS-if.m8}

Also Known As

UAS-αPS2m8

Key Links

Transgenic product class

cDNA

(Brabant et al., 1996)

wild_type

(Brabant et al., 1996)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Brabant et al., 1996)

wild_type

(Brabant et al., 1996)

Mutagen

in vitro construct

(Martin-Bermudo et al., 1997, Brabant et al., 1996, Roote and Zusman, 1996)

Progenitor genotype

Carried in construct

P{UAS-if.m8}

Cytology

Description

The m8 form of if (lacking exon 8) is expressed under the control of UASt regulatory sequences.

(Martin-Bermudo et al., 1997)

UASt regulatory sequences drive expression of cDNA sequences encoding the if 'm8' splice variant.

(Brabant et al., 1996)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

visible | heat sensitive, with Scer\GAL4^337Y

(Baker et al., 2002)

Phenotype Manifest In

wing, with Scer\GAL4⁶⁸⁴

(Kiger et al., 2007)

wing | heat sensitive, with Scer\GAL4^337Y

(Baker et al., 2002)

Detailed Description

Statement

Reference

Adult wings of if^m8.Scer\UAS-expressing flies (under the control of Scer\GAL4⁶⁸⁴) exhibit a significant number of cells that fail to delaminate.

(Kiger et al., 2007)

Animals expressing if^m8.Scer\UAS under the control of Scer\GAL4^337Y usually have wing blisters at 25^o.

(Baker et al., 2002)

Scer\GAL4⁶⁸⁴ induced expression of if^m8.Scer\UAS is temperature sensitive, the penetrance and expressivity of the blister phenotype increases with temperature. Temperature shift experiments reveal the critical period for the generation of wing blisters by overexpression of integrin is at about the time the dorsal and ventral wing epithelia first become apposed. By the time re-apposition is occurring in the pupal period the wing is relatively insensitive to overexpression.

(Brabant et al., 1996)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Scer\GAL4^c684, if^m8.UAS has visible phenotype, suppressible by Dsor1^r2

(Baker et al., 2002)

Scer\GAL4^c684, if^m8.UAS has visible phenotype, suppressible by csw^Esev1A-eOP/csw[+]

(Baker et al., 2002)

Scer\GAL4^c684, if^m8.UAS has visible phenotype, suppressible by msk[+]/msk²

(Baker et al., 2002)

Scer\GAL4^c684, if^m8.UAS has visible phenotype, suppressible by msk⁴/msk[+]

(Baker et al., 2002)

Scer\GAL4^c684, if^m8.UAS has visible phenotype, suppressible by msk[+]/msk⁵

(Baker et al., 2002)

Scer\GAL4^c684, if^m8.UAS has visible phenotype, suppressible by Raf¹

(Baker et al., 2002)

Scer\GAL4^c684, if^m8.UAS has visible phenotype, suppressible by msk¹/msk[+]

(Baker et al., 2002)

Suppressor of

Statement

Reference

if^m8.UAS/Scer\GAL4^48Y is a suppressor of lethal phenotype of mew^M6

(Martin-Bermudo et al., 1997)

Phenotype Manifest In

Suppressed by

Statement

Reference

Scer\GAL4^c684, if^m8.UAS has wing phenotype, suppressible by msk[+]/msk²

(Baker et al., 2002)

Scer\GAL4^c684, if^m8.UAS has wing phenotype, suppressible by msk⁴/msk[+]

(Baker et al., 2002)

Scer\GAL4^c684, if^m8.UAS has wing phenotype, suppressible by msk[+]/msk⁵

(Baker et al., 2002)

Scer\GAL4^c684, if^m8.UAS has wing phenotype, suppressible by Raf¹

(Baker et al., 2002)

Scer\GAL4^c684, if^m8.UAS has wing phenotype, suppressible by Dsor1^r2

(Baker et al., 2002)

Scer\GAL4^c684, if^m8.UAS has wing phenotype, suppressible by csw^Esev1A-eOP/csw[+]

(Baker et al., 2002)

Scer\GAL4^c684, if^m8.UAS has wing phenotype, suppressible by msk¹/msk[+]

(Baker et al., 2002)

Suppressor of

Statement

Reference

if^m8.UAS/Scer\GAL4^hs.PB is a suppressor of photoreceptor neuron phenotype of mew⁴⁹⁸

(Li et al., 1998)

if^m8.UAS/Scer\GAL4^hs.PB is a suppressor of retina | somatic clone phenotype of mew⁴⁹⁸

(Roote and Zusman, 1996)

if^m8.UAS/Scer\GAL4^hs.PB is a suppressor of retina | somatic clone phenotype of mew^M6

(Roote and Zusman, 1996)

Additional Comments

Genetic Interactions

Statement

Reference

The wing blistering caused by expression of if^m8.Scer\UAS under the control of Scer\GAL4^337Y is suppressed by msk¹/+, msk²/+, msk⁴/+, msk⁵/+ or csw^Esev1A-eOP/+.

(Baker et al., 2002)

mew⁴⁹⁸ clones in the eye disrupt photoreceptor organisation. Scer\GAL4^hs.PB-mediated expression of if^m8.Scer\UAS allows some rescue of the phenotype.

(Li et al., 1998)

Partially rescues the first instar lethality of mew^M6 homozygotes when expressed using Scer\GAL4^48Y, although the animals do not survive past the second larval instar stage.

(Martin-Bermudo et al., 1997)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

Scer\GAL4^how-24B/if^m8.UAS rescues if^k27e

(Li et al., 1998)

Scer\GAL4^twi.PG, Scer\GAL4^how-24B, if^m8.UAS rescues if^B4

(Martin-Bermudo et al., 1997)

Scer\GAL4^how-24B/if^m8.UAS rescues if^k27e

(Roote and Zusman, 1996)

Comments

The lethality caused by if^k27e can be rescued by Scer\GAL4^how-24B mediated expression of one copy of a copy of both P{UAS-if.C} and P{UAS-if.m8} or two copies of P{UAS-if.m8}. Expression of one copy of P{UAS-if.m8} fails to rescue lethality, embryos die during development and fail to emerge from the egg cases. These embryos exhibit a muscle phenotype.

(Roote and Zusman, 1996)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

if^m8.Scer\UAS

if^m8.UAS

Name Synonyms

Secondary FlyBase IDs

References (6)

Report Sections

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