FlyBase Allele Report: Dmel\Ser[UAS.cGa]

General Information

Symbol

Dmel\Ser^UAS.cGa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Gu

FlyBase ID

FBal0042742

Feature type

allele

Associated gene

Dmel\Ser

Associated Insertion(s)

Carried in Construct

P{UAS-Ser.G}

Also Known As

UAS-Ser

Key Links

Transgenic product class

wild_type

(Gu et al., 1995)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Gu et al., 1995)

Mutagen

in vitro construct

(Jacobsen et al., 1998, Gu et al., 1995)

Progenitor genotype

Carried in construct

P{UAS-Ser.G}

Cytology

Description

UASt regulatory sequences drive expression of a Ser minigene comprised of cDNA sequence up to position 4037, fused to a 1.2kb genomic fragment encoding the remaining 3' region of the gene.

(Gu et al., 1995)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Ser

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

anterior dorsocentral bristle & trichogen cell, with Scer\GAL4^{l(3)31-1-31-1}

(Jacobsen et al., 1998)

anterior notopleural bristle & trichogen cell, with Scer\GAL4^{l(3)31-1-31-1}

(Jacobsen et al., 1998)

anterior postalar bristle & trichogen cell, with Scer\GAL4^{l(3)31-1-31-1}

(Jacobsen et al., 1998)

anterior scutellar bristle & trichogen cell, with Scer\GAL4^{l(3)31-1-31-1}

(Jacobsen et al., 1998)

anterior supraalar bristle & trichogen cell, with Scer\GAL4^{l(3)31-1-31-1}

(Jacobsen et al., 1998)

dorsal bridge, with Scer\GAL4^arm.PS

(Wiellette and McGinnis, 1999)

dorsal pouch, with Scer\GAL4^arm.PS

(Wiellette and McGinnis, 1999)

embryonic/larval digestive system | embryonic stage, with Scer\GAL4^arm.PS

(Wiellette and McGinnis, 1999)

embryonic/larval proventriculus | embryonic stage, with Scer\GAL4^arm.PS

(Wiellette and McGinnis, 1999)

hub cell | first instar larval stage | increased number, with Scer\GAL4^{VP16.nanos.UTR}

(Okegbe and DiNardo, 2011)

lateral process of cephalopharyngeal skeleton, with Scer\GAL4^arm.PS

(Wiellette and McGinnis, 1999)

microchaeta, with Scer\GAL4^{l(3)31-1-31-1}

(Jacobsen et al., 1998)

mouth hook, with Scer\GAL4^arm.PS

(Wiellette and McGinnis, 1999)

neuron, with Scer\GAL4^hs.PB

(Fleming et al., 1997)

posterior dorsocentral bristle & trichogen cell, with Scer\GAL4^{l(3)31-1-31-1}

(Jacobsen et al., 1998)

posterior notopleural bristle & trichogen cell, with Scer\GAL4^{l(3)31-1-31-1}

(Jacobsen et al., 1998)

posterior postalar bristle & trichogen cell, with Scer\GAL4^{l(3)31-1-31-1}

(Jacobsen et al., 1998)

posterior supraalar bristle & trichogen cell, with Scer\GAL4^{l(3)31-1-31-1}

(Jacobsen et al., 1998)

presumptive embryonic/larval nervous system, with Scer\GAL4^hs.PB

(Hukriede et al., 1997)

presutural bristle & trichogen cell, with Scer\GAL4^{l(3)31-1-31-1}

(Jacobsen et al., 1998)

scutum & macrochaeta & trichogen cell, with Scer\GAL4^{l(3)31-1-31-1}

(Jacobsen et al., 1998)

wing disc ventral compartment, with Scer\GAL4^ptc-559.1

(Fleming et al., 1997)

Detailed Description

Statement

Reference

Expression of Ser^Scer\UAS.cGa under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16} results in an increase in the number of hub cells in the gonad in male larvae compared to controls.

(Okegbe and DiNardo, 2011)

Ser^Scer\UAS.cGa third instar nota clones (under the regulation of Scer\GAL4^{mat.αTub67C.T:Hsim\VP16}) do not affect sensory organ numbers.

(Pitsouli and Delidakis, 2005)

Ubiquitous expression of Ser^Scer\UAS.cGa under the control of Scer\GAL4^arm.PS or Scer\GAL4^64B produces no change in the shape, size or pattern of denticles. Ubiquitous expression of Ser^Scer\UAS.cGa under the control of Scer\GAL4^arm.PS in a Ser^RX106/Df(3R)Bd background results in severe morphological defects; the mouth hooks develop additional sclerotic material at the middle and base and about a quarter of the embryos show duplication of the mouth hook tips.

(Wiellette and McGinnis, 1999)

Flies expressing Ser^Scer\UAS.cGa under the control of Scer\GAL4^{l(3)31-1-31-1} show transformation of the trichogen to a tormogen fate in the notal macrochaetae. The transformation does not affect all notal macrochaetae equally. Some transformation of the microchaetae is seen in these flies.

(Jacobsen et al., 1998)

Scer\GAL4^ptc-559.1-mediated expression causes cell proliferation in the ventral compartment of the wing imaginal disc. Scer\GAL4^Ser.PGF-mediated expression allows nearly normal wing development, adult wings are largely normal with the exception of the distal tip region that shows some double margin formation and ventral overgrowth.

(Fleming et al., 1997)

When expressed via Scer\GAL4^hs.PB in embryos, suppression of neuronal differentiation occurs due to excessive N activity. When expressed via Scer\GAL4^e22c, animals die at approximately the first larval instar but with no gross morphological abnormalities.

(Hukriede et al., 1997)

Scer\GAL4^sca-537.4-mediated expression (causing activation of N) leads to loss of R8 cells (inhibition R8 cell differentiation) and of other photoreceptor cells with incomplete penetrance.

(Baker et al., 1996)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Scer\GAL4^{l(3)31-1-31-1}, Ser^UAS.cGa has visible phenotype, enhanceable by Df(3R)Delta-M2

(Jacobsen et al., 1998)

Suppressed by

Statement

Reference

Scer\GAL4^{l(3)31-1-31-1}, Ser^UAS.cGa has visible phenotype, suppressible by Tp(3;3)bxd¹¹⁰

(Jacobsen et al., 1998)

Scer\GAL4^{l(3)31-1-31-1}, Ser^UAS.cGa has visible phenotype, suppressible by Scer\GAL4^{l(3)31-1-31-1}/fng^UAS.cKa

(Jacobsen et al., 1998)

Scer\GAL4^hs.PB, Ser^UAS.cGa has lethal phenotype, suppressible by fng^UAS.cKa/Scer\GAL4^hs.PB

(Fleming et al., 1997)

Suppressor of

Statement

Reference

Scer\GAL4^bbg-C96/Ser^UAS.cGa is a suppressor of visible phenotype of Scer\GAL4^bbg-C96, mam^N.UAS

(Helms et al., 1999)

Other

Statement

Reference

Scer\GAL4^hs.PB, Ser^UAS.cGa, fng^UAS.cKa has viable phenotype

(Fleming et al., 1997)

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^{l(3)31-1-31-1}, Ser^UAS.cGa has posterior notopleural bristle & trichogen cell phenotype, enhanceable by Df(3R)Delta-M2

(Jacobsen et al., 1998)

Scer\GAL4^{l(3)31-1-31-1}, Ser^UAS.cGa has anterior supraalar bristle & trichogen cell phenotype, enhanceable by Df(3R)Delta-M2

(Jacobsen et al., 1998)

Scer\GAL4^{l(3)31-1-31-1}, Ser^UAS.cGa has posterior supraalar bristle & trichogen cell phenotype, enhanceable by Df(3R)Delta-M2

(Jacobsen et al., 1998)

Scer\GAL4^{l(3)31-1-31-1}, Ser^UAS.cGa has anterior postalar bristle & trichogen cell phenotype, enhanceable by Df(3R)Delta-M2

(Jacobsen et al., 1998)

Scer\GAL4^{l(3)31-1-31-1}, Ser^UAS.cGa has anterior scutellar bristle & trichogen cell phenotype, enhanceable by Df(3R)Delta-M2

(Jacobsen et al., 1998)

Scer\GAL4^{l(3)31-1-31-1}, Ser^UAS.cGa has posterior scutellar bristle & trichogen cell phenotype, enhanceable by Df(3R)Delta-M2

(Jacobsen et al., 1998)

Suppressed by

Statement

Reference

Scer\GAL4^{l(3)31-1-31-1}, Ser^UAS.cGa has presutural bristle & trichogen cell phenotype, suppressible by Tp(3;3)bxd¹¹⁰

(Jacobsen et al., 1998)

Scer\GAL4^{l(3)31-1-31-1}, Ser^UAS.cGa has anterior scutellar bristle & trichogen cell phenotype, suppressible by Tp(3;3)bxd¹¹⁰

(Jacobsen et al., 1998)

Scer\GAL4^{l(3)31-1-31-1}, Ser^UAS.cGa has posterior scutellar bristle & trichogen cell phenotype, suppressible by Tp(3;3)bxd¹¹⁰

(Jacobsen et al., 1998)

Scer\GAL4^{l(3)31-1-31-1}, Ser^UAS.cGa has presutural bristle & trichogen cell phenotype, suppressible by Scer\GAL4^{l(3)31-1-31-1}/fng^UAS.cKa

(Jacobsen et al., 1998)

Scer\GAL4^{l(3)31-1-31-1}, Ser^UAS.cGa has posterior notopleural bristle & trichogen cell phenotype, suppressible by Scer\GAL4^{l(3)31-1-31-1}/fng^UAS.cKa

(Jacobsen et al., 1998)

Scer\GAL4^{l(3)31-1-31-1}, Ser^UAS.cGa has anterior supraalar bristle & trichogen cell phenotype, suppressible by Scer\GAL4^{l(3)31-1-31-1}/fng^UAS.cKa

(Jacobsen et al., 1998)

Scer\GAL4^{l(3)31-1-31-1}, Ser^UAS.cGa has posterior supraalar bristle & trichogen cell phenotype, suppressible by Scer\GAL4^{l(3)31-1-31-1}/fng^UAS.cKa

(Jacobsen et al., 1998)

Scer\GAL4^{l(3)31-1-31-1}, Ser^UAS.cGa has anterior postalar bristle & trichogen cell phenotype, suppressible by Scer\GAL4^{l(3)31-1-31-1}/fng^UAS.cKa

(Jacobsen et al., 1998)

Scer\GAL4^{l(3)31-1-31-1}, Ser^UAS.cGa has anterior dorsocentral bristle & trichogen cell phenotype, suppressible by Scer\GAL4^{l(3)31-1-31-1}/fng^UAS.cKa

(Jacobsen et al., 1998)

Scer\GAL4^{l(3)31-1-31-1}, Ser^UAS.cGa has posterior dorsocentral bristle & trichogen cell phenotype, suppressible by Scer\GAL4^{l(3)31-1-31-1}/fng^UAS.cKa

(Jacobsen et al., 1998)

Scer\GAL4^{l(3)31-1-31-1}, Ser^UAS.cGa has anterior notopleural bristle & trichogen cell phenotype, suppressible by Tp(3;3)bxd¹¹⁰

(Jacobsen et al., 1998)

Scer\GAL4^{l(3)31-1-31-1}, Ser^UAS.cGa has anterior scutellar bristle & trichogen cell phenotype, suppressible by Scer\GAL4^{l(3)31-1-31-1}/fng^UAS.cKa

(Jacobsen et al., 1998)

Scer\GAL4^{l(3)31-1-31-1}, Ser^UAS.cGa has posterior scutellar bristle & trichogen cell phenotype, suppressible by Scer\GAL4^{l(3)31-1-31-1}/fng^UAS.cKa

(Jacobsen et al., 1998)

Scer\GAL4^{l(3)31-1-31-1}, Ser^UAS.cGa has posterior notopleural bristle & trichogen cell phenotype, suppressible by Tp(3;3)bxd¹¹⁰

(Jacobsen et al., 1998)

Scer\GAL4^{l(3)31-1-31-1}, Ser^UAS.cGa has anterior supraalar bristle & trichogen cell phenotype, suppressible by Tp(3;3)bxd¹¹⁰

(Jacobsen et al., 1998)

Scer\GAL4^{l(3)31-1-31-1}, Ser^UAS.cGa has posterior supraalar bristle & trichogen cell phenotype, suppressible by Tp(3;3)bxd¹¹⁰

(Jacobsen et al., 1998)

Scer\GAL4^{l(3)31-1-31-1}, Ser^UAS.cGa has anterior postalar bristle & trichogen cell phenotype, suppressible by Tp(3;3)bxd¹¹⁰

(Jacobsen et al., 1998)

Scer\GAL4^{l(3)31-1-31-1}, Ser^UAS.cGa has posterior postalar bristle & trichogen cell phenotype, suppressible by Tp(3;3)bxd¹¹⁰

(Jacobsen et al., 1998)

Scer\GAL4^{l(3)31-1-31-1}, Ser^UAS.cGa has anterior dorsocentral bristle & trichogen cell phenotype, suppressible by Tp(3;3)bxd¹¹⁰

(Jacobsen et al., 1998)

Scer\GAL4^{l(3)31-1-31-1}, Ser^UAS.cGa has posterior dorsocentral bristle & trichogen cell phenotype, suppressible by Tp(3;3)bxd¹¹⁰

(Jacobsen et al., 1998)

Enhancer of

Statement

Reference

Ser^UAS.cGa/Scer\GAL4^{VP16.mat.αTub67C} is an enhancer of sensory mother cell | third instar larval stage | ectopic phenotype of Delta^UAS.cLa

(Pitsouli and Delidakis, 2005)

Suppressor of

Statement

Reference

Ser^UAS.cGa/Scer\GAL4^{VP16.mat.αTub67C} is a suppressor of sensory mother cell | ectopic | somatic clone | third instar larval stage phenotype of Delta^RevF10

(Pitsouli and Delidakis, 2005)

Additional Comments

Genetic Interactions

Statement

Reference

Dl^Scer\UAS.cLa, Ser^Scer\UAS.cGa double-mutant third instar nota clones (under the regulation of Scer\GAL4^{mat.αTub67C.T:Hsim\VP16}) typically contain ten or more ectopic sensory organ precursors. Addition of the Ser^Scer\UAS.cGa transgene, to Dl^RevF10 Ser^RX106 double mutant third instar nota clones, under the control of Scer\GAL4^{mat.αTub67C.T:Hsim\VP16}, partially rescues the ectopic SOP phenotype, with approximately 26% of SOP positions exhibiting between one and four SOPs.

(Pitsouli and Delidakis, 2005)

Weakly suppresses the wing nicking phenotype seen in mam^N.Scer\UAS, Scer\GAL4^C96 flies.

(Helms et al., 1999)

The transformation of the trichogen to a tormogen fate in the notal macrochaetae caused by Ser^Scer\UAS.cGa expressed under the control of Scer\GAL4^{l(3)31-1-31-1} is enhanced by Df(3R)Dl-M2 and suppressed by Tp(3;3)bxd¹¹⁰. The bristle transformation phenotype seen when both Ser^Scer\UAS.cGa and Dl^Scer\UAS.cJa are expressed under the control of Scer\GAL4^{l(3)31-1-31-1} appears to be additive. The transformation of the trichogen to a tormogen fate in the notal macrochaetae caused by Ser^Scer\UAS.cGa expressed under the control of Scer\GAL4^{l(3)31-1-31-1} is suppressed by co-expression of fng^Scer\UAS.cKa.

(Jacobsen et al., 1998)

Expression in a fng^- background causes dorsal cell proliferation (displacing the dorsal/ventral margin). Scer\GAL4^hs.PB-mediated expression causes death prior to the end of embryogenesis. Mutants display severely reduced neuronal differentiation. Simultaneous expression with fng^Scer\UAS.cKa reduces lethality associated with ectopic expression of Ser and restores normal neuronal differentiation, flies develop into phenotypically wild type adults.

(Fleming et al., 1997)

When expressed via Scer\GAL4^hs.PB in a Df(3R)Dl-BX6 background, neuronal hyperplasia is reduced.

(Hukriede et al., 1997)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

Scer\GAL4^Ser.6.PY/Ser^UAS.cGa rescues Ser^Bd-3/Ser^+r83k

(Yan et al., 2004)

Scer\GAL4^Ser.7.PY/Ser^UAS.cGa rescues Ser^Bd-3/Ser^+r83k

(Yan et al., 2004)