FlyBase Allele Report: Dmel\hh[N.UAS]

General Information

Symbol

Dmel\hh^N.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0045882

Feature type

allele

Associated gene

Dmel\hh

Associated Insertion(s)

Carried in Construct

P{UAS-hh.N}

Also Known As

UAS-hhN, UAS-hh-N, UAS-Hh^N

Key Links

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Forbes et al., 1996, Porter et al., 1996, Therond et al., 1996, Porter et al., 1995)

Progenitor genotype

hh^UAS.cAa

(Porter et al., 1996)

Carried in construct

P{UAS-hh.N}

Cytology

Description

UAS regulatory sequences drive expression of an N-terminal fragment (amino acid residues 1-257) of hh.

(Porter et al., 1995)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal - all die during embryonic stage, with Scer\GAL4^en-e16E

(Porter et al., 1996)

some die during embryonic stage, with Scer\GAL4^en-e16E

(Porter et al., 1996)

Phenotype Manifest In

dorsal thick hair, with Scer\GAL4^en-e16E

(Lum, 2003)

embryonic/larval cuticle, with Scer\GAL4^en-e16E

(Lum, 2003)

embryonic dorsal epidermis, with Scer\GAL4^en-e16E

(Porter et al., 1996)

embryonic ventral epidermis, with Scer\GAL4^en-e16E

(Porter et al., 1996)

follicle cell, with Scer\GAL4^hs.PB

(Forbes et al., 1996)

germarium region 2a, with Scer\GAL4^hs.PB

(Forbes et al., 1996)

germarium region 2b, with Scer\GAL4^hs.PB

(Forbes et al., 1996)

oocyte, with Scer\GAL4^hs.PB

(Forbes et al., 1996)

ovariole, with Scer\GAL4^hs.PB

(Forbes et al., 1996)

polar follicle cell, with Scer\GAL4^hs.PB

(Forbes et al., 1996)

scutellar bristle, with Scer\GAL4^en-e16E

(Porter et al., 1996)

ventral denticle belt, with Scer\GAL4^en-e16E

(Porter et al., 1996)

wing | anterior, with Scer\GAL4^unspecified

(Ramirez-Weber et al., 2001)

wing disc, with Scer\GAL4^en-e16E

(Aza-Blanc et al., 1997)

wing vein, with Scer\GAL4^en-e16E

(Porter et al., 1996)

wing vein, with Scer\GAL4^unspecified

(Ramirez-Weber et al., 2001)

wing vein L3, with Scer\GAL4^unspecified

(Ramirez-Weber et al., 2001)

wing vein L4, with Scer\GAL4^unspecified

(Ramirez-Weber et al., 2001)

Detailed Description

Statement

Reference

Overexpression of hh^N.Scer\UAS (under the regulation of Scer\GAL4^en-e16E) results in conversion of tertiary fates (thick, pigmented hair) to ectopic secondary fates (smooth cuticle) on the dorsal epidermis of larvae.

(Lum, 2003)

When hh^N.Scer\UAS is driven by Scer\GAL4^unspecified the anterior wing is affected. Morphology and vein patterns are affected.

(Ramirez-Weber et al., 2001)

Scer\GAL4^en-e16E-mediated expression causes grossly enlarged and misshapen wing discs.

(Aza-Blanc et al., 1997)

Ectopic expression of hh^N.Scer\UAS results in very similar phenotypes to those caused by ectopic expression of the full length protein, from hh^hs.PI.

(Forbes et al., 1996)

When expression is driven by Scer\GAL4^en-e16E, ventral cuticle defects include a rectangular rather than a trapezoidal shape for the denticle belts and a loss of denticle diversity, and dorsal cuticle defects include a loss of tertiary and an expansion of secondary cell types. The phenotype is essentially identical to that caused by ubiquitous expression of a full length or N-truncated hh gene product.

(Porter et al., 1996)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Suppressed by

Statement

Reference

Scer\GAL4^en-e16E, hh^N.UAS has dorsal thick hair phenotype, suppressible by cos²

(Lum, 2003)

Scer\GAL4^en-e16E, hh^N.UAS has embryonic/larval cuticle phenotype, suppressible by cos²

(Lum, 2003)

Scer\GAL4^unspecified, hh^N.UAS has wing vein phenotype, suppressible by fu¹⁴

(Ramirez-Weber et al., 2001)

Scer\GAL4^unspecified, hh^N.UAS has wing | anterior phenotype, suppressible by fu¹⁴

(Ramirez-Weber et al., 2001)

Additional Comments

Genetic Interactions

Statement

Reference

The reduction in eye size seen in upd1^unspecified/Df(1)os1A2 flies is increased by expression of hh^N.Scer\UAS under the control of Scer\GAL4^ey.PH and extra bristles are seen in the eye.

(Bach et al., 2003)

Overexpression of hh^N.Scer\UAS under the regulation of Scer\GAL4^en-e16E, in cos² mutants fails to convert tertiary fates (thick, pigmented hair) to ectopic secondary fates (smooth cuticle) on the dorsal epidermis of larvae.

(Lum, 2003)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Feitz et al.

(Forbes et al., 1996)

Comments

Expressed in S2 cells and used to produce conditioned medium containing hh protein to study its effect on ci-mediated transcriptional activation.

(Chen et al., 1999)

Carried in a plasmid and expressed in S2 cells. Used to produce a conditioned medium, which has been used to study the response of cl-8 cells to hh signalling.

(Chen et al., 1999)

Expressed in S2 cells.

(Nybakken et al., 2002)

Carried in a plasmid and transfected into S2 cells.

(Zhu et al., 2003)

Carried in a plasmid and expressed in Schneider line 2 cells. The N cleavage product, in the absence of the C cleavage product.

(Porter et al., 1995)

Carried in plasmid "Hh-N", expressed in Schneider S2 cells that normally express fu and not hh to test the signalling from hh to fu in cultured cells.

(Therond et al., 1996)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Hh-N

(Chen et al., 1999)

hh^N.Scer\UAS

hh^N.UAS

Name Synonyms

Secondary FlyBase IDs

References (24)

Report Sections

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