FlyBase Allele Report: Dmel\hh[UAS.cAa]

General Information

Symbol

Dmel\hh^UAS.cAa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Azpiazu

FlyBase ID

FBal0057274

Feature type

allele

Associated gene

Dmel\hh

Associated Insertion(s)

Carried in Construct

P{UAS-hh.A}

Also Known As

UAS-hh

Key Links

Transgenic product class

cDNA

(Azpiazu et al., 1996)

wild_type

(Azpiazu et al., 1996)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Azpiazu et al., 1996)

wild_type

(Azpiazu et al., 1996)

Mutagen

in vitro construct

(Hazelett et al., 1998, Azpiazu et al., 1996, Porter et al., 1996)

Progenitor genotype

Carried in construct

P{UAS-hh.A}

Cytology

Description

UASt regulatory sequences drive expression of hh cDNA 11.

(Azpiazu et al., 1996)

Expression of full length hh protein is governed by UAS regulatory sequences.

(Porter et al., 1996)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

UASt

binary expression system - regulatory region

Encoded product / tool

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy, with Scer\GAL4^sim.P3.7

(Watson et al., 2011)

visible, with Scer\GAL4^71B

(Su et al., 2007)

Phenotype Manifest In

embryonic dorsal epidermis, with Scer\GAL4^en-e16E

(Porter et al., 1996)

embryonic ventral epidermis, with Scer\GAL4^en-e16E

(Porter et al., 1996)

follicle cell, with Scer\GAL4^C587

(Eliazer et al., 2014)

glial cell & eye disc | somatic clone | cell non-autonomous, with Scer\GAL4^Act5C.PP

(Rangarajan et al., 2001)

midline glial cell, with Scer\GAL4^sim.P3.7

(Watson et al., 2011)

scutellar bristle, with Scer\GAL4^en-e16E

(Porter et al., 1996)

stalk follicle cell | increased number, with Scer\GAL4^hs.PB

(King et al., 2001)

wing, with Scer\GAL4^71B

(Su et al., 2007)

wing vein, with Scer\GAL4^en-e16E

(Porter et al., 1996)

Detailed Description

Statement

Reference

Expressing hh^UAS.cAa under the control of Scer\GAL4^C587 leads to follicle cell defects.

(Eliazer et al., 2014)

Midline glia do not completely ensheath the axon commissures, and there is an absence of membrane projections into the commissures in stage 15 embryos expressing hh^Scer\UAS.cAa under the control of Scer\GAL4^sim.P3.7.

(Watson et al., 2011)

Expression of hh^Scer\UAS.cAa under the control of Scer\GAL4^71B results in merging of wing veins L2 and L3.

(Su et al., 2007)

Females with hh^Scer\UAS.cAa, driven by Scer\GAL4^hs.PB (and heatshocked) have an increased number of stalk cells (six-fold more than wild-type).

(King et al., 2001)

When hh^Scer\UAS.cAa is expressed in clones in the developing eye (under the control of Scer\GAL4^Act5C.PP) a normal number of glial cells is seen in the eye disc and the optic stalk. However glial cells that overshoot past the axonal boundary are seen, usually in streams a few cells wide, and without regard to the position of the clone.

(Rangarajan et al., 2001)

When expression is driven by Scer\GAL4^en-e16E, only slight differences from wild type are observed in the ventral and dorsal embryonic cuticle pattern. Embryos go on to hatch and form adults that are normal except for wing venation defects and supernumerary scutellar bristles.

(Porter et al., 1996)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Other

Statement

Reference

Scer\GAL4^bi-omb-Gal4, dpp^UAS.cSa, ey^UAS.cHa, hh^UAS.cAa has lethal | larval stage phenotype

(Kango-Singh et al., 2003)

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^Act5C.PI, ey^UAS.cHa, hh^UAS.cAa has wing disc | somatic clone phenotype, enhanceable by dpp^UAS.cSa/Scer\GAL4^Act5C.PI

(Kango-Singh et al., 2003)

NOT Suppressor of

Statement

Reference

hh^UAS.cAa/Scer\GAL4^dpp.blk1 is a non-suppressor of eye disc morphogenetic furrow phenotype of Scer\GAL4^dpp.blk1, wg^wg.3'UTR.UAS

(Cadigan et al., 2002)

hh^UAS.cAa/Scer\GAL4^ey.PH is a non-suppressor of photoreceptor neuron phenotype of kto^T631

(Treisman, 2001)

hh^UAS.cAa/Scer\GAL4^ey.PH is a non-suppressor of eye disc phenotype of kto^T631

(Treisman, 2001)

hh^UAS.cAa/Scer\GAL4^ey.PH is a non-suppressor of eye disc phenotype of skd^T13

(Treisman, 2001)

hh^UAS.cAa/Scer\GAL4^ey.PH is a non-suppressor of photoreceptor neuron phenotype of skd^T13

(Treisman, 2001)

hh^UAS.cAa/Scer\GAL4^ey.PH is a non-suppressor of photoreceptor neuron phenotype of kto^T241

(Treisman, 2001)

hh^UAS.cAa/Scer\GAL4^ey.PH is a non-suppressor of eye disc phenotype of kto^T241

(Treisman, 2001)

hh^UAS.cAa/Scer\GAL4^ey.PH is a non-suppressor of eye disc phenotype of skd^T413

(Treisman, 2001)

hh^UAS.cAa/Scer\GAL4^ey.PH is a non-suppressor of photoreceptor neuron phenotype of skd^T413

(Treisman, 2001)

hh^UAS.cAa/Scer\GAL4^ey.PH is a non-suppressor of photoreceptor neuron phenotype of kto^T555

(Treisman, 2001)

hh^UAS.cAa/Scer\GAL4^ey.PH is a non-suppressor of eye disc phenotype of kto^T555

(Treisman, 2001)

hh^UAS.cAa/Scer\GAL4^ey.PH is a non-suppressor of eye disc phenotype of skd^T606

(Treisman, 2001)

hh^UAS.cAa/Scer\GAL4^ey.PH is a non-suppressor of photoreceptor neuron phenotype of skd^T606

(Treisman, 2001)

Other

Statement

Reference

Scer\GAL4^bi-omb-Gal4, ci^{Ce-2.UAS.Tag:HA}, hh^UAS.cAa has wing disc phenotype

(Kango-Singh et al., 2003)

Scer\GAL4^bi-omb-Gal4, ci^{Ce-2.UAS.Tag:HA}, hh^UAS.cAa has eye disc morphogenetic furrow | ectopic phenotype

(Kango-Singh et al., 2003)

Scer\GAL4^Act5C.PI, dpp^UAS.cSa, ey^UAS.cHa, hh^UAS.cAa has wing disc | ectopic | somatic clone phenotype

(Kango-Singh et al., 2003)

Scer\GAL4^Act5C.PI, ey^UAS.cHa, hh^UAS.cAa has eye | ectopic | somatic clone phenotype

(Kango-Singh et al., 2003)

Scer\GAL4^Act5C.PI, ey^UAS.cHa, hh^UAS.cAa has wing disc | somatic clone phenotype

(Kango-Singh et al., 2003)

Scer\GAL4^30A, ey^UAS.cHa, hh^UAS.cAa has eye | ectopic phenotype

(Kango-Singh et al., 2003)

Scer\GAL4^dpp.blk1, hh^UAS.cAa, wg^wg.3'UTR.UAS has eye disc morphogenetic furrow | ectopic phenotype

(Cadigan et al., 2002)

Scer\GAL4^dpp.blk1, hh^UAS.cAa, wg^wg.3'UTR.UAS has eye disc morphogenetic furrow phenotype

(Cadigan et al., 2002)

Additional Comments

Genetic Interactions

Statement

Reference

When hh^Scer\UAS.cAa and ey^Scer\UAS.cHa are expressed in clones (under the control of Scer\GAL4^Act5C.PI) in the anterior compartment of the wing disc, 46% of clones cause ectopic eye formation. When hh^Scer\UAS.cAa and ey^Scer\UAS.cHa are expressed under the control of Scer\GAL4^30A in the anterior compartment of the wing disc, ectopic eye formation is seen at 100% penetrance, although the size of these ectopic eyes varies from disc to disc. When ci^{Ce-2.Scer\UAS.T:Ivir\HA1} and hh^Scer\UAS.cAa co-expressed (driven by Scer\GAL4^bi-omb-Gal4) in the wing disc leads to overgrowth and ectopic morphogenetic furrow initiation. When dpp^Scer\UAS.cSa, hh^Scer\UAS.cAa and ey^Scer\UAS.cHa are co-expressed in clones (driven by Scer\GAL4^Act5C.PI), very strong overgrowth and distorted morphology is seen in the wing disc. Co-expression of dpp^Scer\UAS.cSa, hh^Scer\UAS.cAa and ey^Scer\UAS.cHa driven by Scer\GAL4^bi-omb-Gal4, causes a high larval lethality at 25^oC.

(Kango-Singh et al., 2003)

Co-expression of wg^{wg.3'UTR.Scer\UAS} and hh^Scer\UAS.cAa under the control of Scer\GAL4^dpp.blk1 does not rescue morphogenetic furrow progression from the posterior edge of the eye disc, but usually results in one (or occasionally more) ectopic morphogenetic furrows initiating in the interior of the eye primordium and progressing concentrically.

(Cadigan et al., 2002)

eyg¹/Df(3L)iro-2 hemizygous eye discs completely lack photoreceptors. Photoreceptor development in these discs is not rescued by hh^Scer\UAS.cAa expressed under the control of Scer\GAL4^dpp.PH. The lack of photoreceptor phenotype of eya¹ eye discs is not rescued by hh^Scer\UAS.cAa expressed under the control of Scer\GAL4^ey.PH.

(Hazelett et al., 1998)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

hh^UAS.cAa/Scer\GAL4^GMR.PF rescues hh^bar3

(Chou and Chien, 2002)

Scer\GAL4^en-e16E/hh^UAS.cAa rescues hh^ts2

(Chamoun et al., 2001)

Comments

hh^Scer\UAS.cAa driven with Scer\GAL4^GMR.PF, rescues morphogenetic furrow progression and ommatidial over-rotation defects in hh^bar3 mutants.

(Chou and Chien, 2002)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

98448

y¹ w^*; P{UAS-hh.A}b

Notes on Origin

Discoverer

Comments

Carried in a plasmid and transfected into S2R+ cells.

(Zhang et al., 2004)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

hh^Scer\UAS.cAa

hh^UAS.cAa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Azpiazu

(Azpiazu et al., 1996)

Secondary FlyBase IDs

FBal0051517

References (33)

Report Sections

Open Close