FlyBase Allele Report: Dmel\E(spl)m8-HLH[UAS.cTa]

General Information

Symbol

Dmel\E(spl)m8-HLH^UAS.cTa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Tata

FlyBase ID

FBal0052454

Feature type

allele

Associated gene

Dmel\E(spl)m8-HLH

Associated Insertion(s)

Carried in Construct

P{UAS-E(spl)m8-HLH.T}

Also Known As

UAS-E(spl)m8, UAS-m8

Key Links

Transgenic product class

wild_type

(Tata and Hartley, 1995)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Tata and Hartley, 1995)

Mutagen

in vitro construct

(Tata and Hartley, 1995)

Progenitor genotype

Carried in construct

P{UAS-E(spl)m8-HLH.T}

Cytology

Description

Expression of the E(spl)m8-HLH coding region is governed by UASt regulatory sequences.

(Tata and Hartley, 1995)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

E(spl)m8-HLH

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

visible, with Scer\GAL4^dpp.blk1

(Li et al., 2009, Nolo et al., 2000)

visible, with Scer\GAL4^sca-C253

(Barry et al., 2011)

Phenotype Manifest In

campaniform sensillum L3-2 of wing vein L3, with Scer\GAL4^hs.PB

(Tata and Hartley, 1995)

campaniform sensillum L3-3 of wing vein L3, with Scer\GAL4^hs.PB

(Tata and Hartley, 1995)

campaniform sensillum of anterior crossvein, with Scer\GAL4^hs.PB

(Tata and Hartley, 1995)

chaeta, with Scer\GAL4^sca-C253

(Barry et al., 2011)

chemosensory ventral triple row bristle, with Scer\GAL4^hs.PB

(Tata and Hartley, 1995)

chordotonal organ precursor cell & ventral thoracic disc, with Scer\GAL4^sca-109-68

(zur Lage and Jarman, 1999)

dorsal row of triple row of wing sensilla, with Scer\GAL4^hs.PB

(Tata and Hartley, 1995)

eo neuron, with Scer\GAL4^hs.PB

(Tata and Hartley, 1995)

macrochaeta, with Scer\GAL4^hs.PB

(Tata and Hartley, 1995)

microchaeta, with Scer\GAL4^hs.PB

(Tata and Hartley, 1995)

scutellar bristle, with Scer\GAL4^dpp.blk1

(Li et al., 2009, Nolo et al., 2000)

wing vein, with Scer\GAL4^hs.PB

(Tata and Hartley, 1995)

Detailed Description

Statement

Reference

Expression of E(spl)^Scer\UAS.cTa under the control of Scer\GAL4^sca-C253 results in a loss of thoracic bristles in the adult fly.

(Barry et al., 2011)

Flies expressing E(spl)^Scer\UAS.cTa under the control of Scer\GAL4^dpp.blk1 have missing scutellar bristles.

(Li et al., 2009)

Flies expressing E(spl)^Scer\UAS.cTa under the control of Scer\GAL4^dpp.blk1 show loss of scutellar bristles.

(Nolo et al., 2000)

Formation of sensory organ precursors (SOPs) of the femoral chordotonal sense organ is suppressed in leg discs in which E(spl)^Scer\UAS.cTa is expressed under the control of Scer\GAL4^sca-109-68.

(zur Lage and Jarman, 1999)

When expression is driven by Scer\GAL4^hs.PB, with two heat pulses of 45 minutes 8-24hr APF, adult flies display a variety of bristle phenotypes in two categories: bristle organ loss and aberrant bristle structures (e.g. double sockets, split shaft, multiple shafts or stunted/deformed bristle shafts). Earlier heat pulses promote bristle loss, later heat pulses promote aberrant bristles. When expression is driven by Scer\GAL4^hs.PB, induced between 8 and 14 hr APF, microchaetae fail to develop. The neurons in the nota are correspondingly reduced or absent. When expression is driven by Scer\GAL4^hs.PB, heat shock applied to late third instar larvae leads to a marked loss of notum macrochaetae, campaniform sensillae of the anterior crossvein and the third wing vein, and chemosensory bristles on the wing margin. When expression is driven by Scer\GAL4^hs.PB, heat shocked at 15hr APF, gaps appear in the wing veins.

(Tata and Hartley, 1995)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

E(spl)m8-HLH^UAS.cTa, Scer\GAL4^sca-C253 has visible phenotype, suppressible | partially by E(spl)m8-HLH^UAS.cTa, Scer\GAL4^sca-C253

(Barry et al., 2011)

NOT suppressed by

Statement

Reference

E(spl)m8-HLH^UAS.cTa, Scer\GAL4^sca-C253 has visible phenotype, non-suppressible by dgrn^HCAA.UASp, Scer\GAL4^sca-C253

(Barry et al., 2011)

E(spl)m8-HLH^UAS.cTa, Scer\GAL4^sca-C253 has visible phenotype, non-suppressible by dgrn^ΔSIMs.UASp, Scer\GAL4^sca-C253

(Barry et al., 2011)

Suppressor of

Statement

Reference

E(spl)m8-HLH^UAS.cTa, Scer\GAL4^sca-C253 is a suppressor | partially of visible phenotype of E(spl)m8-HLH^UAS.cTa, Scer\GAL4^sca-C253

(Barry et al., 2011)

Phenotype Manifest In

Suppressed by

Statement

Reference

E(spl)m8-HLH^UAS.cTa, Scer\GAL4^sca-C253 has chaeta phenotype, suppressible | partially by E(spl)m8-HLH^UAS.cTa, Scer\GAL4^sca-C253

(Barry et al., 2011)

NOT suppressed by

Statement

Reference

E(spl)m8-HLH^UAS.cTa, Scer\GAL4^sca-C253 has chaeta phenotype, non-suppressible by dgrn^HCAA.UASp, Scer\GAL4^sca-C253

(Barry et al., 2011)

E(spl)m8-HLH^UAS.cTa, Scer\GAL4^sca-C253 has chaeta phenotype, non-suppressible by dgrn^ΔSIMs.UASp, Scer\GAL4^sca-C253

(Barry et al., 2011)

Suppressor of

Statement

Reference

E(spl)m8-HLH^UAS.cTa, Scer\GAL4^sca-C253 is a suppressor | partially of chaeta phenotype of E(spl)m8-HLH^UAS.cTa, Scer\GAL4^sca-C253

(Barry et al., 2011)

E(spl)m8-HLH^UAS.cTa is a suppressor of macrochaeta & wing | ectopic phenotype of Mmus\Neurog1^UAS.cQa, Scer\GAL4^dpp.blk1

(Quan et al., 2004)

E(spl)m8-HLH^UAS.cTa, Scer\GAL4^dpp.blk1 is a suppressor of chaeta phenotype of Scer\GAL4^dpp.blk1, sens^UAS.cNa

(Nolo et al., 2000)

Additional Comments

Genetic Interactions

Statement

Reference

The loss of thoracic bristles seen in adults expressing E(spl)^Scer\UAS.cTa under the control of Scer\GAL4^sca-C253 is suppressed by co-expression of dgrn^{Scer\UAS.P\T.cBa} but is not suppressed by co-expression of either dgrn^{HCAA.Scer\UAS.P\T} or dgrn^{ΔSIMs.Scer\UAS.P\T}.

(Barry et al., 2011)

Expression of both sens^Scer\UAS.cNa and E(spl)^Scer\UAS.cTa under the control of Scer\GAL4^dpp.blk1 results in a strong reduction in supernumerary bristles (seen when sens^Scer\UAS.cNa is expressed alone under the control of Scer\GAL4^dpp.blk1) in most areas and occasionally loss of bristles.

(Nolo et al., 2000)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

26872

w^*; P{UAS-E(spl)m8-HLH.T}3

Notes on Origin

Discoverer

F. Tata.

(de Celis et al., 1996)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

E(spl)^Scer\UAS.cTa

E(spl)^UAS.cTa

E(spl)m8-HLH^Scer\UAS.cTa

E(spl)m8-HLH^UAS.cTa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Tata

(Tata and Hartley, 1995)

Secondary FlyBase IDs

References (13)

Report Sections

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