FB2024_03 , released June 25, 2024
Allele: Dmel\dlg1UAS.cBa
Open Close
General Information
Symbol
Dmel\dlg1UAS.cBa
Species
D. melanogaster
Name
Saccharomyces cerevisiae UAS construct a of Budnik
FlyBase ID
FBal0057607
Feature type
allele
Associated gene
Dmel\dlg1
Associated Insertion(s)
Carried in Construct
P{UAS-dlg1.B}
Also Known As
UAS-Dlg
Key Links
Transgenic product class
cDNA
(Budnik et al., 1996, Woods et al., 1996)
wild_type
(Budnik et al., 1996, Woods et al., 1996)
Mutagen
Nature of the Allele
Transgenic product class
cDNA
(Budnik et al., 1996, Woods et al., 1996)
wild_type
(Budnik et al., 1996, Woods et al., 1996)
Mutagen
in vitro construct
(Budnik et al., 1996, Woods et al., 1996)
Progenitor genotype
Carried in construct
P{UAS-dlg1.B}
Cytology
Description

UASt regulatory sequences drive expression of a complete dlg1 cDNA (coding sequence for the A transcript).

(Budnik et al., 1996)

UASt regulatory sequences drive expression of a dlg1A cDNA fragment that contains the complete coding region plus 143bp of 5' and 40bp of 3' untranslated sequence.

(Woods et al., 1996)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
dlg1
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      The subsynaptic reticulum underlying larval neuromuscular junctions is expanded in dlg1Scer\UAS.cBa; Scer\GAL4BG487.

      (Gorczyca et al., 2007)

      Scer\GAL4BG487 mediated expression in wild type causes overdevelopment of the subsynaptic reticulum (SSR) and results in a larger than normal immunoreactive area around type I boutons.

      (Budnik et al., 1996)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Suppressed by
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      The expansion of the subsynaptic reticulum of larval neuromuscular junctions due to dlg1Scer\UAS.cBa; Scer\GAL4BG487 is partially suppressed by co-expression of Syx18Scer\UAS.cGa.

      (Gorczyca et al., 2007)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescues

      dlg1UAS.cBa/Scer\GAL4BG487 rescues dlg114

      (Tejedor et al., 1997)

      Scer\GAL4sca.PC/dlg1UAS.cBa rescues dlg114

      (Budnik et al., 1996)

      Scer\GAL4BG380/dlg1UAS.cBa rescues dlg114

      (Budnik et al., 1996)

      Scer\GAL432B/dlg1UAS.cBa rescues dlg112

      (Woods et al., 1996)

      Scer\GAL432B/dlg1UAS.cBa rescues dlg114

      (Woods et al., 1996)

      Scer\GAL432B/dlg1UAS.cBa rescues dlg17

      (Woods et al., 1996)
      Partially rescues

      dlg1UAS.cBa/Scer\GAL4BG487 partially rescues dlg114/Df(1)N71

      (Karunanithi et al., 2002)

      Scer\GAL4BG380/dlg1UAS.cBa partially rescues dlg114/Df(1)N71

      (Karunanithi et al., 2002)

      Scer\GAL4unspecified/dlg1UAS.cBa partially rescues dlg17

      (Thomas et al., 1997)

      dlg1UAS.cBa/Scer\GAL4BG487 partially rescues dlg114

      (Budnik et al., 1996)

      dlg1UAS.cBa/Scer\GAL4C57 partially rescues dlg114

      (Budnik et al., 1996)
      Comments

      Expression of dlg1Scer\UAS.cBa, driven by either Scer\GAL4BG380 or Scer\GAL4BG487, rescues the neurological phenotype of dlg114/Df(1)N71 mutants, leading to wild-type vesicle and quantal size. The synaptic area is reduced to a wild-type size in type 1s boutons, while in type 1b boutons, the rescued synaptic area is actually smaller than wild-type controls.

      (Karunanithi et al., 2002)

      Scer\GAL4BG487 or Scer\GAL4C57 mediated postsynaptic expression partially rescues SSR mutant phenotype, Scer\GAL4sca.PC or Scer\GAL4BG380 mediated presynaptic expression fully rescues.

      (Budnik et al., 1996)

      Rescues lethality of dlg114, dlg112 and dlg17 when expressed from Scer\GAL432B.

      (Woods et al., 1996)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      dlg1Scer\UAS.cBa
      dlg1UAS.cBa
      Name Synonyms
      Saccharomyces cerevisiae UAS construct a of Budnik
      (Budnik et al., 1996)
      Secondary FlyBase IDs
        References (8)