FlyBase Allele Report: Dmel\Dr[UAS.cIa]

General Information

Symbol

Dmel\Dr^UAS.cIa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Isshiki

FlyBase ID

FBal0063863

Feature type

allele

Associated gene

Dmel\Dr

Associated Insertion(s)

Carried in Construct

P{UAS-msh}

Also Known As

UAS-msh

Key Links

Transgenic product class

cDNA

(Isshiki et al., 1997)

wild_type

(Isshiki et al., 1997)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Isshiki et al., 1997)

wild_type

(Isshiki et al., 1997)

Mutagen

in vitro construct

(Isshiki et al., 1997)

Progenitor genotype

Carried in construct

P{UAS-msh}

Cytology

Description

An Dr cDNA containing the entire open reading frame together with 236bp of 5' and 124bp of 3' untranslated sequences is expressed under the control of UASt regulatory sequences.

(Isshiki et al., 1997)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal, with Scer\GAL4^GMR.PF

(Mozer, 2001)

lethal, with Scer\GAL4^h-1J3

(Mozer, 2001)

lethal | heat sensitive, with Scer\GAL4^hs.PB

(Mozer, 2001)

lethal - all die before end of pupal stage | somatic clone | heat sensitive, with Scer\GAL4^ap-md544

(Villa-Cuesta and Modolell, 2005)

Minute | somatic clone, with Scer\GAL4^ap-md544

(Villa-Cuesta and Modolell, 2005)

some die during pupal stage | somatic clone | heat sensitive, with Scer\GAL4^ap-md544

(Villa-Cuesta and Modolell, 2005)

viable, with Scer\GAL4^dpp.blk1

(Mozer, 2001)

viable, with Scer\GAL4^ey.PB

(Mozer, 2001)

visible, with Scer\GAL4^ap-md544

(Milan et al., 2001)

visible, with Scer\GAL4^C-765

(Milan et al., 2001)

visible, with Scer\GAL4^ey.PB

(Mozer, 2001)

visible | heat sensitive, with Scer\GAL4^hs.PB

(Mozer, 2001)

Phenotype Manifest In

A1-7 ventral longitudinal muscle cell, with Scer\GAL4^how-24B

(Nose et al., 1998)

alula | ventral, with Scer\GAL4^C-765

(Milan et al., 2001)

cardioblast, with Scer\GAL4^how-24B

(Jagla et al., 2002)

dorsal acute muscle cell, with Scer\GAL4^how-24B

(Nose et al., 1998)

dorsal oblique muscle cell, with Scer\GAL4^how-24B

(Nose et al., 1998)

dorsal transverse muscle cell, with Scer\GAL4^how-24B

(Nose et al., 1998)

embryonic pericardial cell, with Scer\GAL4^how-24B

(Jagla et al., 2002)

eye, with Scer\GAL4^ey.PB

(Mozer, 2001)

eye, with Scer\GAL4^GMR.PF

(Mozer, 2001)

eye disc morphogenetic furrow, with Scer\GAL4^hs.PB

(Mozer, 2001)

heart primordium, with Scer\GAL4^how-24B

(Jagla et al., 2002)

hypodermal muscle cell of larval abdominal 9, with Scer\GAL4^how-24B

(Nose et al., 1998)

interommatidial bristle, with Scer\GAL4^hs.PB

(Mozer, 2001)

larval DA1 motor neuron, with Scer\GAL4^sca-537.4

(Isshiki et al., 1997)

larval fpCC neuron, with Scer\GAL4^sca-537.4

(Isshiki et al., 1997)

larval longitudinal connective, with Scer\GAL4^sca-537.4

(Isshiki et al., 1997)

larval RP2 motor neuron, with Scer\GAL4^sca-537.4

(Isshiki et al., 1997)

lens, with Scer\GAL4^hs.PB

(Mozer, 2001)

mesothoracic tergum | pharate adult stage | somatic clone, with Scer\GAL4^ap-md544

(Villa-Cuesta and Modolell, 2005)

muscle cell of A1-7 dorsal acute muscle 1, with Scer\GAL4^how-24B

(Jagla et al., 2002)

muscle cell of A1-7 dorsal oblique muscle 1 | ectopic, with Scer\GAL4^how-24B

(Jagla et al., 2002)

muscle cell of A1-7 ventral oblique muscle 1, with Scer\GAL4^how-24B

(Nose et al., 1998)

muscle cell of A1-7 ventral oblique muscle 2, with Scer\GAL4^how-24B

(Nose et al., 1998)

muscle cell of abdominal 1 dorsal acute muscle 3, with Scer\GAL4^how-24B

(Nose et al., 1998)

muscle cell of abdominal 1 dorsal oblique muscle 3, with Scer\GAL4^how-24B

(Nose et al., 1998)

muscle cell of abdominal 1 dorsal oblique muscle 4, with Scer\GAL4^how-24B

(Nose et al., 1998)

muscle cell of abdominal 1 dorsal oblique muscle 5, with Scer\GAL4^how-24B

(Nose et al., 1998)

muscle cell of abdominal 1 dorsal transverse muscle 1, with Scer\GAL4^how-24B

(Nose et al., 1998)

muscle cell of abdominal 1 lateral longitudinal muscle 1, with Scer\GAL4^how-24B

(Nose et al., 1998)

muscle cell of abdominal 1 lateral transverse muscle 1, with Scer\GAL4^how-24B

(Nose et al., 1998)

muscle cell of abdominal 1 lateral transverse muscle 2, with Scer\GAL4^how-24B

(Nose et al., 1998)

muscle cell of abdominal 1 lateral transverse muscle 3, with Scer\GAL4^how-24B

(Nose et al., 1998)

muscle cell of abdominal 1 lateral transverse muscle 4, with Scer\GAL4^how-24B

(Nose et al., 1998)

muscle cell of abdominal 2 dorsal acute muscle 3, with Scer\GAL4^how-24B

(Nose et al., 1998)

muscle cell of abdominal 2 dorsal oblique muscle 3, with Scer\GAL4^how-24B

(Nose et al., 1998)

muscle cell of abdominal 2 dorsal oblique muscle 4, with Scer\GAL4^how-24B

(Nose et al., 1998)

muscle cell of abdominal 2 dorsal oblique muscle 5, with Scer\GAL4^how-24B

(Nose et al., 1998)

muscle cell of abdominal 2 dorsal transverse muscle 1, with Scer\GAL4^how-24B

(Nose et al., 1998)

muscle cell of abdominal 2 lateral longitudinal muscle 1, with Scer\GAL4^how-24B

(Nose et al., 1998)

muscle cell of abdominal 2 lateral transverse muscle 1, with Scer\GAL4^how-24B

(Nose et al., 1998)

muscle cell of abdominal 2 lateral transverse muscle 2, with Scer\GAL4^how-24B

(Nose et al., 1998)

muscle cell of abdominal 2 lateral transverse muscle 3, with Scer\GAL4^how-24B

(Nose et al., 1998)

muscle cell of abdominal 2 lateral transverse muscle 4, with Scer\GAL4^how-24B

(Nose et al., 1998)

muscle cell of abdominal 3 dorsal acute muscle 3, with Scer\GAL4^how-24B

(Nose et al., 1998)

muscle cell of abdominal 3 dorsal oblique muscle 3, with Scer\GAL4^how-24B

(Nose et al., 1998)

muscle cell of abdominal 3 dorsal oblique muscle 4, with Scer\GAL4^how-24B

(Nose et al., 1998)

muscle cell of abdominal 3 dorsal oblique muscle 5, with Scer\GAL4^how-24B

(Nose et al., 1998)

muscle cell of abdominal 3 dorsal transverse muscle 1, with Scer\GAL4^how-24B

(Nose et al., 1998)

muscle cell of abdominal 3 lateral longitudinal muscle 1, with Scer\GAL4^how-24B

(Nose et al., 1998)

muscle cell of abdominal 3 lateral transverse muscle 1, with Scer\GAL4^how-24B

(Nose et al., 1998)

muscle cell of abdominal 3 lateral transverse muscle 2, with Scer\GAL4^how-24B

(Nose et al., 1998)

muscle cell of abdominal 3 lateral transverse muscle 3, with Scer\GAL4^how-24B

(Nose et al., 1998)

muscle cell of abdominal 3 lateral transverse muscle 4, with Scer\GAL4^how-24B

(Nose et al., 1998)

muscle cell of abdominal 4 dorsal acute muscle 3, with Scer\GAL4^how-24B

(Nose et al., 1998)

muscle cell of abdominal 4 dorsal oblique muscle 3, with Scer\GAL4^how-24B

(Nose et al., 1998)

muscle cell of abdominal 4 dorsal oblique muscle 4, with Scer\GAL4^how-24B

(Nose et al., 1998)

muscle cell of abdominal 4 dorsal oblique muscle 5, with Scer\GAL4^how-24B

(Nose et al., 1998)

muscle cell of abdominal 4 dorsal transverse muscle 1, with Scer\GAL4^how-24B

(Nose et al., 1998)

muscle cell of abdominal 4 lateral longitudinal muscle 1, with Scer\GAL4^how-24B

(Nose et al., 1998)

muscle cell of abdominal 4 lateral transverse muscle 1, with Scer\GAL4^how-24B

(Nose et al., 1998)

muscle cell of abdominal 4 lateral transverse muscle 2, with Scer\GAL4^how-24B

(Nose et al., 1998)

muscle cell of abdominal 4 lateral transverse muscle 3, with Scer\GAL4^how-24B

(Nose et al., 1998)

muscle cell of abdominal 4 lateral transverse muscle 4, with Scer\GAL4^how-24B

(Nose et al., 1998)

muscle cell of abdominal 5 dorsal acute muscle 3, with Scer\GAL4^how-24B

(Nose et al., 1998)

muscle cell of abdominal 5 dorsal oblique muscle 3, with Scer\GAL4^how-24B

(Nose et al., 1998)

muscle cell of abdominal 5 dorsal oblique muscle 4, with Scer\GAL4^how-24B

(Nose et al., 1998)

muscle cell of abdominal 5 dorsal oblique muscle 5, with Scer\GAL4^how-24B

(Nose et al., 1998)

muscle cell of abdominal 5 dorsal transverse muscle 1, with Scer\GAL4^how-24B

(Nose et al., 1998)

muscle cell of abdominal 5 lateral longitudinal muscle 1, with Scer\GAL4^how-24B

(Nose et al., 1998)

muscle cell of abdominal 5 lateral transverse muscle 1, with Scer\GAL4^how-24B

(Nose et al., 1998)

muscle cell of abdominal 5 lateral transverse muscle 2, with Scer\GAL4^how-24B

(Nose et al., 1998)

muscle cell of abdominal 5 lateral transverse muscle 3, with Scer\GAL4^how-24B

(Nose et al., 1998)

muscle cell of abdominal 5 lateral transverse muscle 4, with Scer\GAL4^how-24B

(Nose et al., 1998)

muscle cell of abdominal 6 dorsal acute muscle 3, with Scer\GAL4^how-24B

(Nose et al., 1998)

muscle cell of abdominal 6 dorsal oblique muscle 3, with Scer\GAL4^how-24B

(Nose et al., 1998)

muscle cell of abdominal 6 dorsal oblique muscle 4, with Scer\GAL4^how-24B

(Nose et al., 1998)

muscle cell of abdominal 6 dorsal oblique muscle 5, with Scer\GAL4^how-24B

(Nose et al., 1998)

muscle cell of abdominal 6 dorsal transverse muscle 1, with Scer\GAL4^how-24B

(Nose et al., 1998)

muscle cell of abdominal 6 lateral longitudinal muscle 1, with Scer\GAL4^how-24B

(Nose et al., 1998)

muscle cell of abdominal 6 lateral transverse muscle 1, with Scer\GAL4^how-24B

(Nose et al., 1998)

muscle cell of abdominal 6 lateral transverse muscle 2, with Scer\GAL4^how-24B

(Nose et al., 1998)

muscle cell of abdominal 6 lateral transverse muscle 3, with Scer\GAL4^how-24B

(Nose et al., 1998)

muscle cell of abdominal 6 lateral transverse muscle 4, with Scer\GAL4^how-24B

(Nose et al., 1998)

muscle cell of abdominal 7 dorsal acute muscle 3, with Scer\GAL4^how-24B

(Nose et al., 1998)

muscle cell of abdominal 7 dorsal oblique muscle 3, with Scer\GAL4^how-24B

(Nose et al., 1998)

muscle cell of abdominal 7 dorsal oblique muscle 4, with Scer\GAL4^how-24B

(Nose et al., 1998)

muscle cell of abdominal 7 dorsal oblique muscle 5, with Scer\GAL4^how-24B

(Nose et al., 1998)

muscle cell of abdominal 7 dorsal transverse muscle 1, with Scer\GAL4^how-24B

(Nose et al., 1998)

muscle cell of abdominal 7 lateral longitudinal muscle 1, with Scer\GAL4^how-24B

(Nose et al., 1998)

muscle cell of abdominal 7 lateral transverse muscle 1, with Scer\GAL4^how-24B

(Nose et al., 1998)

muscle cell of abdominal 7 lateral transverse muscle 2, with Scer\GAL4^how-24B

(Nose et al., 1998)

muscle cell of abdominal 7 lateral transverse muscle 3, with Scer\GAL4^how-24B

(Nose et al., 1998)

muscle cell of abdominal 7 lateral transverse muscle 4, with Scer\GAL4^how-24B

(Nose et al., 1998)

muscle cell of abdominal 8 dorsal transverse muscle 1, with Scer\GAL4^how-24B

(Nose et al., 1998)

muscle cell of mesothoracic dorsal acute muscle 3, with Scer\GAL4^how-24B

(Nose et al., 1998)

muscle cell of mesothoracic dorsal oblique muscle 3, with Scer\GAL4^how-24B

(Nose et al., 1998)

muscle cell of mesothoracic dorsal oblique muscle 4, with Scer\GAL4^how-24B

(Nose et al., 1998)

muscle cell of mesothoracic lateral longitudinal muscle 1, with Scer\GAL4^how-24B

(Nose et al., 1998)

muscle cell of mesothoracic lateral transverse muscle 1, with Scer\GAL4^how-24B

(Nose et al., 1998)

muscle cell of mesothoracic lateral transverse muscle 2, with Scer\GAL4^how-24B

(Nose et al., 1998)

muscle cell of mesothoracic lateral transverse muscle 3, with Scer\GAL4^how-24B

(Nose et al., 1998)

muscle cell of mesothoracic lateral transverse muscle 4, with Scer\GAL4^how-24B

(Nose et al., 1998)

muscle cell of metathoracic dorsal acute muscle 3, with Scer\GAL4^how-24B

(Nose et al., 1998)

muscle cell of metathoracic dorsal oblique muscle 3, with Scer\GAL4^how-24B

(Nose et al., 1998)

muscle cell of metathoracic dorsal oblique muscle 4, with Scer\GAL4^how-24B

(Nose et al., 1998)

muscle cell of metathoracic lateral longitudinal muscle 1, with Scer\GAL4^how-24B

(Nose et al., 1998)

muscle cell of metathoracic lateral transverse muscle 1, with Scer\GAL4^how-24B

(Nose et al., 1998)

muscle cell of metathoracic lateral transverse muscle 2, with Scer\GAL4^how-24B

(Nose et al., 1998)

muscle cell of metathoracic lateral transverse muscle 3, with Scer\GAL4^how-24B

(Nose et al., 1998)

muscle cell of metathoracic lateral transverse muscle 4, with Scer\GAL4^how-24B

(Nose et al., 1998)

muscle cell of prothoracic dorsal oblique muscle 3, with Scer\GAL4^how-24B

(Nose et al., 1998)

muscle cell of prothoracic dorsal oblique muscle 4, with Scer\GAL4^how-24B

(Nose et al., 1998)

muscle cell of ventral oblique muscle 4, with Scer\GAL4^how-24B

(Nose et al., 1998)

muscle cell of ventral oblique muscle 5, with Scer\GAL4^how-24B

(Nose et al., 1998)

muscle cell of ventral oblique muscle 6, with Scer\GAL4^how-24B

(Nose et al., 1998)

pCC neuron, with Scer\GAL4^sca-537.4

(Isshiki et al., 1997)

segment border muscle cell, with Scer\GAL4^how-24B

(Jagla et al., 1999)

symmetrical commissure, with Scer\GAL4^sca-537.4

(Isshiki et al., 1997)

U neuron, with Scer\GAL4^sca-537.4

(Isshiki et al., 1997)

ventral midline of embryo, with Scer\GAL4^sca-537.4

(Isshiki et al., 1997)

wing | dorsal, with Scer\GAL4^ap-md544

(Milan et al., 2001)

wing | pharate adult stage | somatic clone, with Scer\GAL4^ap-md544

(Villa-Cuesta and Modolell, 2005)

wing margin | anterior | ventral, with Scer\GAL4^C-765

(Milan et al., 2001)

wing sensillum | ectopic, with Scer\GAL4^ap-md544

(Milan et al., 2001)

wing vein, with Scer\GAL4^ap-md544

(Milan et al., 2001)

wing vein L3 | ventral, with Scer\GAL4^C-765

(Milan et al., 2001)

Detailed Description

Statement

Reference

Overexpression of Dr^Scer\UAS.cIa, under the regulation of Scer\GAL4^ap-md544 in pharate adults results in reduced and crumpled wings, and a reduced notum. However, no hinge-like sensory organs or other structures could be discerned in the latter. Overexpression of Dr^Scer\UAS.cIa, under the regulation of Scer\GAL4^ap-md544 in the whole dorsal compartment of the disc at 17^oC reduces the size of the notum territory. At 25^oC, it strongly inhibits the development of this territory and individuals die at the pupal stages.

(Villa-Cuesta and Modolell, 2005)

When Dr^Scer\UAS.cIa is driven by Scer\GAL4^how-24B alterations are seen both dorsal muscle and heart cell populations. The number of heart cells is dramatically reduced. The majority of pericardial cells and about 70% of cardioblasts are lacking. The majority of eve expressing DA1 muscle precursors are substituted by supernumerary DO1 muscles.

(Jagla et al., 2002)

The anterior wing margin differentiates dorsal-type bristles arranged in a dorsal-like pattern on both surfaces in flies expressing Dr^Scer\UAS.cIa under the control of Scer\GAL4^C-765. The pattern of the vein L3 is symmetric, with a dorsal corrugation pattern on both surfaces. Few bristles are seen on the ventral surface of the alula, suggesting transformation to a dorsal fate. Expression of Dr^Scer\UAS.cIa under the control of Scer\GAL4^ap-md544 reduces the size of the dorsal wing pouch and induces differentiation of ectopic bristles in the wing blade. Vein differentiation is also affected.

(Milan et al., 2001)

A small fraction of flies expressing Dr^Scer\UAS.cIa under the control of Scer\GAL4^ey.PB show mild roughening of the eye. Expression of Dr^Scer\UAS.cIa under the control of Scer\GAL4^dpp.blk1 has no effect on eye development. Expression of Dr^Scer\UAS.cIa under the control of Scer\GAL4^h-1J3 results in lethality prior to the third larval instar. Expression of Dr^Scer\UAS.cIa under the control of Scer\GAL4^GMR.PF results in lethality prior to eclosion. Rare escapers exhibit severe roughening of the eye. Approximately 25% of eye discs in which Dr^Scer\UAS.cIa is expressed under the control of Scer\GAL4^hs.PB using a 1 hour heat shock (followed by a 4 hour recovery) show an arrest of the morphogenetic furrow.

(Mozer, 2001)

The segmental border muscles are absent and the number of Kr-expressing muscles increases in embryos expressing Dr^Scer\UAS.cIa under the control of Scer\GAL4^how-24B.

(Jagla et al., 1999)

Scer\GAL4^how-24B-mediated expression severely alters muscle morphology, muscle may be completely missing or exhibit abnormal shape and orientation. Less severe phenotype is seen in the dorsal region, muscles are thicker than normal and occasionally contain additional muscles. Ventral internal muscles are also disorganised. Lateral transverse muscles form normally but their morphology is somewhat irregular. Ectopic Dr expression interferes with the correct specification of the progenitors/founders.

(Nose et al., 1998)

Produces severe disruptions of the axon tracts in embryos, when expressed using Scer\GAL4^sca-537.4; the commissures are almost completely absent and the longitudinal connectives are broken and interrupted. Gaps are often seen along the midline in these embryos. The aCC, pCC, CQ, fpCC and RP2 neurons fail to differentiate.

(Isshiki et al., 1997)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Scer\GAL4^ap-md544/Dr^UAS.cIa is a suppressor | partially of visible phenotype of ap^md544/ap^UGO35

(Milan et al., 2001)

Phenotype Manifest In

Suppressor of

Statement

Reference

Scer\GAL4^ap-md544/Dr^UAS.cIa is a suppressor | partially of wing margin phenotype of ap^md544/ap^UGO35

(Milan et al., 2001)

NOT Suppressor of

Statement

Reference

Scer\GAL4^ap-md544/Dr^UAS.cIa is a non-suppressor of wing phenotype of ap^md544/ap^UGO35

(Milan et al., 2001)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of Dr^Scer\UAS.cIa under the control of Scer\GAL4^ap-md544 in ap^md544/ap^UGO35 flies does not restore outgrowth of the wing, although the few margin bristles seen in the dorsal surface of these wings have dorsal identity. Co-expression of fng^EP3082 and Dr^Scer\UAS.cIa under the control of Scer\GAL4^ap-md544 in ap^md544/ap^UGO35 flies results in dorsal differentiation in bristles of the dorsal anterior wing margin.

(Milan et al., 2001)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

Scer\GAL4^how-24B/Dr^UAS.cIa rescues Dr^Δ68

(Nose et al., 1998)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

Dr^Scer\UAS.cIa

Dr^UAS.cIa

msh^Scer\UAS.cIa

(Nose et al., 1998)

msh^UAS.cIa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Isshiki

(Isshiki et al., 1997)

Secondary FlyBase IDs

References (13)

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