FlyBase Allele Report: Dmel\Ser[Bd.UAS.Tag:MYC]

General Information

Symbol

Dmel\Ser^{Bd.UAS.Tag:MYC}

Species

D. melanogaster

Name

FlyBase ID

FBal0065007

Feature type

allele

Associated gene

Dmel\Ser

Associated Insertion(s)

Carried in Construct

P{UAS-Bd.H}

Also Known As

UAS-Ser^DN

Key Links

Transgenic product class

dominant_negative_variant

(Hukriede et al., 1997)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

dominant_negative_variant

(Hukriede et al., 1997)

Mutagen

in vitro construct

(Hukriede et al., 1997)

Progenitor genotype

Ser^Bd-3

(Hukriede et al., 1997)

Carried in construct

P{UAS-Bd.H}

Cytology

Description

Expression of coding region of Ser^Bd-3, tagged with Tag:MYC, is governed by UASt regulatory sequences.

(Hukriede et al., 1997)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Ser

Tagged with

Tag:MYC

epitope tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

embryonic epidermis, with Scer\GAL4^hs.PB

(Hukriede et al., 1997)

embryonic epidermis | embryonic stage, with Scer\GAL4^hs.PB

(Hukriede et al., 1997)

embryonic head, with Scer\GAL4^e22c

(Hukriede et al., 1997)

eye | somatic clone, with Scer\GAL4^Act5C.PP

(Chern and Choi, 2002)

eye | ventral, with Scer\GAL4^ey.PH

(Singh et al., 2006)

eye disc, with Scer\GAL4^ey.PH

(Singh and Choi, 2003)

eye disc | dorsal, with Scer\GAL4^bi-omb-Gal4

(Singh et al., 2004)

eye disc | somatic clone, with Scer\GAL4^Act5C.PP

(Chern and Choi, 2002)

eye disc | ventral, with Scer\GAL4^bi-omb-Gal4

(Singh et al., 2004)

eye disc | ventral, with Scer\GAL4^ey.PH

(Singh et al., 2006)

leg, with Scer\GAL4^ptc-559.1

(Hukriede et al., 1997)

mouth hook, with Scer\GAL4^e22c

(Hukriede et al., 1997)

presumptive embryonic/larval nervous system, with Scer\GAL4^hs.PB

(Hukriede et al., 1997)

wing, with Scer\GAL4^ptc-559.1

(Hukriede et al., 1997)

wing, with Scer\GAL4^Ser.PGF

(Hukriede et al., 1997)

wing disc | larval stage, with Scer\GAL4^ptc-559.1

(Hukriede et al., 1997)

wing disc dorsal compartment, with Scer\GAL4^Ser.PGF

(Hukriede et al., 1997)

wing margin, with Scer\GAL4^Ser.PGF

(Hukriede et al., 1997)

Detailed Description

Statement

Reference

Expression of Ser^{Bd.Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^ey.PH results in the loss of the ventral-half of the eye, both at the eye disc and the adult eye level.

(Singh et al., 2006)

Expression of Ser^{Bd.Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^bi-omb-Gal4 results in suppression of eye development on both the ventral and dorsal margins in the eye disc.

(Singh et al., 2004)

Expression of Ser^{Bd.Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^ey.PH during 12-72 hours of development (using a shift to 29^oC from 18^oC) generally results in complete elimination of the eye field in the imaginal disc, although antennal development is not affected in the disc. Some extremely small eye discs with a few photoreceptors are also seen. Expression of Ser^{Bd.Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^ey.PH during 72-96 hours of development (using a shift to 29^oC from 18^oC) shows a significant reduction in the no-eye phenotype along with an increase in frequency of selective suppression of the ventral eye compared to expression during 12-72 hours of development. Expression of Ser^{Bd.Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^ey.PH during 96-168 hours of development (using a shift to 29^oC from 18^oC) shows a sharp increase in the selective loss of ventral eye phenotype.

(Singh and Choi, 2003)

Eye discs that contain clones expressing Ser^{Bd.Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^Act5C.PP are variably reduced. In the most severe cases this results in rudimentary eyes. In some cases a preferential reduction of the ventral eye is seen. Ventral clones in the eye disc expressing Ser^{Bd.Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^Act5C.PP are only rarely recovered.

(Chern and Choi, 2002)

When expressed via Scer\GAL4^e22c, animals die at approximately the first larval instar with head and mouth hook abnormalities remarkably similar to those of Ser^rev6-1. When expressed via Scer\GAL4^Ser.PGF or Scer\GAL4^Ser.PGF flies exhibit wing margin loss phenotypes. The severity of the phenotype is greater for Scer\GAL4^Ser.PGF than for Scer\GAL4^Ser.PGF. The wing margin phenotype for Ser^{Bd.Scer\UAS.T:Hsap\MYC}, Scer\GAL4^Ser.PGF is more severe in the presence of two wild type Ser alleles than is the wing phenotype associated with the Ser^Bd-3 heterozygous for a single Ser⁺ allele. When expressed via Scer\GAL4^Ser.PGF in a Ser^+r83k background the flies have phenotypes indistinguishable from Ser^Bd-3/Ser^+r83k mutants. When expressed via Scer\GAL4^hs.PB in embryos, lethality results, with neuronal hypertrophy at the expense of hypodermal differentiation. When expressed via Scer\GAL4^Ser.PGF, the regions of the wing imaginal disc corresponding to the presumptive wing blade are consistently smaller and misshapen relative to wild type. When expressed via Scer\GAL4^ptc-559.1, flies die during early pupal formation prior to imaginal disc eversion.

(Hukriede et al., 1997)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Scer\GAL4^hs.PB, Ser^{Bd.UAS.Tag:MYC} has lethal phenotype, suppressible | partially by fng^UAS.cKa/Scer\GAL4^hs.PB

(Hukriede et al., 1997)

NOT Suppressor of

Statement

Reference

Ser^{Bd.UAS.Tag:MYC}, Scer\GAL4^ptc-559.1 is a non-suppressor of abnormal size | larval stage phenotype of Delta^UAS.cJa, Scer\GAL4^ptc-559.1

(Hukriede et al., 1997)

Other

Statement

Reference

Scer\GAL4^ptc-559.1, Ser^{Bd.UAS.Tag:MYC}, fng^UAS.cKa has lethal | pharate adult stage phenotype

(Hukriede et al., 1997)

Scer\GAL4^ptc-559.1, Ser^{Bd.UAS.Tag:MYC}, fng^UAS.cKa has abnormal size | pharate adult stage phenotype

(Hukriede et al., 1997)

Phenotype Manifest In

Suppressed by

Statement

Reference

Scer\GAL4^ey.PH, Ser^{Bd.UAS.Tag:MYC} has eye | ventral phenotype, suppressible by BacA\p35^UAS.cHa, Scer\GAL4^ey.PH

(Singh et al., 2006)

Scer\GAL4^ey.PH, Ser^{Bd.UAS.Tag:MYC} has eye disc | ventral phenotype, suppressible by BacA\p35^UAS.cHa, Scer\GAL4^ey.PH

(Singh et al., 2006)

Scer\GAL4^bi-omb-Gal4, Ser^{Bd.UAS.Tag:MYC} has eye disc | ventral phenotype, suppressible | partially by tsh^UAS.cGa/Scer\GAL4^bi-omb-Gal4

(Singh et al., 2004)

Scer\GAL4^bi-omb-Gal4, Ser^{Bd.UAS.Tag:MYC} has eye disc | dorsal phenotype, suppressible | partially by tsh^UAS.cGa/Scer\GAL4^bi-omb-Gal4

(Singh et al., 2004)

NOT Suppressor of

Statement

Reference

Ser^{Bd.UAS.Tag:MYC}, Scer\GAL4^ptc-559.1 is a non-suppressor of wing disc ventral compartment | larval stage phenotype of Delta^UAS.cJa, Scer\GAL4^ptc-559.1

(Hukriede et al., 1997)

Other

Statement

Reference

Scer\GAL4^ptc-559.1, Ser^{Bd.UAS.Tag:MYC}, fng^UAS.cKa has leg | pharate adult stage phenotype

(Hukriede et al., 1997)

Scer\GAL4^ptc-559.1, Ser^{Bd.UAS.Tag:MYC}, fng^UAS.cKa has wing blade | pharate adult stage phenotype

(Hukriede et al., 1997)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of tsh^Scer\UAS.cGa partially rescues the suppression of eye development on both the ventral and dorsal margins of the eye that is seen in animals expressing Ser^{Bd.Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^bi-omb-Gal4.

(Singh et al., 2004)

Expression of Ser^{Bd.Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^ey.PH at 25^oC in eye discs homozygous for pnr^VX6 (generated using the "EGUF" method to remove all eye disc cells except the homozygous pnr^VX6 clone cells) results in a complete loss of the eye in 99% of flies, compared to 50% of flies showing complete loss of the eye or a very small eye when Ser^{Bd.Scer\UAS.T:Hsap\MYC} is expressed under the control of Scer\GAL4^ey.PH at 25^oC in a wild-type background. Expression of Ser^{Bd.Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^ey.PH at 25^oC in eye discs homozygous for Df(3L)iro-DFM3 (generated using the "EGUF" method to remove all eye disc cells except the homozygous Df(3L)iro-DFM3 clone cells) results in a complete loss of the eye in 99% of flies, compared to 50% of flies showing complete loss of the eye or a very small eye when Ser^{Bd.Scer\UAS.T:Hsap\MYC} is expressed under the control of Scer\GAL4^ey.PH at 25^oC in a wild-type background.

(Singh and Choi, 2003)

When expressed via Scer\GAL4^hs.PB in a Df(3R)Dl-BX6 background, no reduction in the severity of the neurogenic phenotype is observed. Coexpression of Dl^Scer\UAS.cJa and Ser^{Bd.Scer\UAS.T:Hsap\MYC} via Scer\GAL4^Ser.PGF did not alter the extent of cell proliferation in the dorsal wing compartment in discs or adult wing blades. Coexpression of Dl^Scer\UAS.cJa and Ser^{Bd.Scer\UAS.T:Hsap\MYC} via Scer\GAL4^ptc-559.1 leads to variable but consistent reduction in the extent of ventral compartment cell proliferation as compared to that produced by Dl^Scer\UAS.cJa alone. The antimorphic effects of Ser^{Bd.Scer\UAS.T:Hsap\MYC} (therefore Ser^Bd-3) are compartmentally restricted in the wing disc. Coexpression of Ser^{Bd.Scer\UAS.T:Hsap\MYC} and fng^Scer\UAS.cKa via Scer\GAL4^hs.PB results in animals with apparently normal CNS development, and apparently improved viability when compared to Ser^{Bd.Scer\UAS.T:Hsap\MYC}, Scer\GAL4^hs.PB alone. When co-expressed with fng^Scer\UAS.cKa via Scer\GAL4^ptc-559.1, flies survive longer, to late pupal stages, after disc eversion and as pharate adults. The flies cannot eclose but exhibit complete though shortened legs and no wing blades.

(Hukriede et al., 1997)

Xenogenetic Interactions

Statement

Reference

Expression of BacA\p35^Scer\UAS.cHa under the control of Scer\GAL4^ey.PH significantly rescues the loss of the ventral-half of they eye in 32% Scer\GAL4^ey.PH>Ser^{Bd.Scer\UAS.T:Hsap\MYC} flies.

(Singh et al., 2006)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Ser^{Bd.Scer\UAS.T:Hsap\MYC}

Ser^Bd.UAS.T:Myc

Ser^{Bd.UAS.Tag:MYC}

Name Synonyms

Secondary FlyBase IDs

References (6)

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