FlyBase Allele Report: Dmel\mira[L44]

General Information

Symbol

Dmel\mira^L44

Species

D. melanogaster

Name

FlyBase ID

FBal0082443

Feature type

allele

Associated gene

Dmel\mira

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

loss of function allele

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

loss of function allele

(Irion et al., 2006, Ikeshima-Kataoka et al., 1997)

Mutagen

ethyl methanesulfonate

(Ikeshima-Kataoka et al., 1997)

Progenitor genotype

Cytology

Description

Nucleotide 544 (G) has been replaced with CAC, which produces a frameshift after amino acid residue 103 (the protein produced contains 33 novel amino acid residues, which are PLRLPVTRIRPPEAEVAPQQRHPLLCIRQPPRP).

(Fuerstenberg et al., 1998)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

delins

3R:19,934,363..19,934,363 [-]

Inserted_sequence:

CAC

Comment:

The G in codon 104 of mira has been replaced with CAC leading to a frameshift and early translation termination after the addition of 33 novel amino acids. The last wild type amino acid is F103.

(Fuerstenberg et al., 1998)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy

(Berger et al., 2010)

lethal | recessive

(Ikeshima-Kataoka et al., 1997)

Phenotype Manifest In

embryonic/larval neuron

(Akiyama-Oda et al., 2000)

glial cell | increased number

(Berger et al., 2010)

larval longitudinal connective

(Ikeshima-Kataoka et al., 1997)

symmetrical commissure

(Ikeshima-Kataoka et al., 1997)

thoracic neuroblast NB6-4

(Akiyama-Oda et al., 2000)

Detailed Description

Statement

Reference

mira^L44 mutants exhibit ectopic gcm expression and an increase in glial number (4-5) accompanied by the lack of the neuronal progeny.

(Berger et al., 2010)

All progeny of NB6-4T adopt a glial cell fate, expressing gcm and repo and moving towards the midline.

(Akiyama-Oda et al., 2000)

Axon tracts of embryos are grossly defective, longitudinal connectives are generally broken or severely reduced in width and commissures are partially or completely fused.

(Ikeshima-Kataoka et al., 1997)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

mira^L44 has abnormal neuroanatomy phenotype, suppressible by Scer\GAL4^sca-109-68/CycE^UAS.cBa

(Berger et al., 2010)

Phenotype Manifest In

Suppressed by

Statement

Reference

mira^L44 has glial cell | increased number phenotype, suppressible by Scer\GAL4^sca-109-68/CycE^UAS.cBa

(Berger et al., 2010)

Enhancer of

Statement

Reference

mira^L44/mira[+] is an enhancer of egg chamber phenotype of cup²¹

(Piccioni et al., 2009)

mira^L44/mira[+] is an enhancer of egg chamber phenotype of cup¹⁵

(Piccioni et al., 2009)

Other

Statement

Reference

mira^L44, pros^m4 has embryonic/larval glial cell phenotype

(Akiyama-Oda et al., 2000)

mira^L44, pros^m4 has thoracic neuroblast NB6-4 phenotype

(Akiyama-Oda et al., 2000)

mira^L44, pros^m4 has neuroblast NB7-4 phenotype

(Akiyama-Oda et al., 2000)

Additional Comments

Genetic Interactions

Statement

Reference

Over-expression of CycE^Scer\UAS.cBa under the control of Scer\GAL4^sca-109-68 in a mira^L44 background is sufficient to confer neuronal fate to the NB6-4t lineage.

(Berger et al., 2010)

The ovarian phenotype of cup¹⁵ and cup²¹ homozygous females is enhanced if they are also heterozygous for mira^L44; a greater fraction of egg chambers arrest development between stages 3 and 5 in the double mutant ovaries compared to in the single mutant ovaries.

(Piccioni et al., 2009)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

mira^RR127

(Ikeshima-Kataoka et al., 1997)

mira^ZZ176

(Ikeshima-Kataoka et al., 1997)

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

66670

w^*; P{neoFRT}82B mira^L44 e^*/TM6B, P{Dfd-EYFP}3, Sb¹ Tb¹ ca¹

Notes on Origin

Discoverer

Comments

One of a group of six alleles isolated in the same screen.

(Ikeshima-Kataoka et al., 1997)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Mir^L44

(Fuerstenberg et al., 1998)

mira^L44

(Wu et al., 2023, Hannaford et al., 2018, Ramat et al., 2017, Bivik et al., 2015, Berger et al., 2010, Piccioni et al., 2009, Griffiths et al., 2007, Betschinger et al., 2006, Irion et al., 2006, Lee et al., 2006)

miranda^L44

(Fuerstenberg et al., 1998)

Name Synonyms

Secondary FlyBase IDs

References (14)

Report Sections

Open Close