FlyBase Allele Report: Dmel\H[UAS.cGa]

General Information

Symbol

Dmel\H^UAS.cGa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Go

FlyBase ID

FBal0090635

Feature type

allele

Associated gene

Dmel\H

Associated Insertion(s)

Carried in Construct

P{UAS-H.G}

Also Known As

UAS-H, UAS-Hairless

Key Links

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Go et al., 1998)

Progenitor genotype

Carried in construct

P{UAS-H.G}

Cytology

Description

The entire H open reading frame is expressed under the control of UASt regulatory sequences.

(Go et al., 1998)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal - all die before end of P-stage, with Scer\GAL4^sca-537.4

(Barolo et al., 2002)

some die during pharate adult stage, with Scer\GAL4^sca-537.4

(Barolo et al., 2002)

visible, with Scer\GAL4^ey.PH

(Lee et al., 2009)

visible, with Scer\GAL4^hs.PB

(Barolo et al., 2000)

visible, with Scer\GAL4^sca-537.4

(Barolo et al., 2002)

visible, with Scer\GAL4^Su(H).ASE5

(Barolo et al., 2002)

visible, with Scer\GAL4^T113

(Go et al., 1998)

visible, with Scer\GAL4^vg.int2.1

(Go et al., 1998)

Phenotype Manifest In

adult thorax, with Scer\GAL4^T113

(Go et al., 1998)

adult thorax & macrochaeta, with Scer\GAL4^T113

(Go et al., 1998)

chaeta, with Scer\GAL4^sca-537.4

(Barolo et al., 2002)

chaeta, with Scer\GAL4^Su(H).ASE5

(Barolo et al., 2002)

embryonic hindgut, with Scer\GAL4^byn-Gal4

(Iwaki and Lengyel, 2002, Takashima et al., 2002)

external sensory organ precursor cell | increased number, with Scer\GAL4^{E(spl)mα-BFM.1.0}

(Castro et al., 2005)

eye, with Scer\GAL4^ey.PH

(Lee et al., 2009, Chao et al., 2004)

eye, with Scer\GAL4^T113

(Go et al., 1998)

eye disc, with Scer\GAL4^T113

(Go et al., 1998)

haltere, with Scer\GAL4^T113

(Go et al., 1998)

tormogen cell, with Scer\GAL4^hs.PB

(Barolo et al., 2000)

tormogen cell, with Scer\GAL4^sca-537.4

(Barolo et al., 2002)

tormogen cell, with Scer\GAL4^Su(H).ASE5

(Barolo et al., 2002)

trichogen cell | ectopic, with Scer\GAL4^{E(spl)mα-BFM.1.0}

(Castro et al., 2005)

trichogen cell | ectopic, with Scer\GAL4^hs.PB

(Barolo et al., 2000)

trichogen cell | ectopic, with Scer\GAL4^sca-537.4

(Barolo et al., 2002)

trichogen cell | ectopic, with Scer\GAL4^Su(H).ASE5

(Barolo et al., 2002)

wing, with Scer\GAL4^T113

(Go et al., 1998)

wing, with Scer\GAL4^vg.int2.1

(Go et al., 1998)

wing disc, with Scer\GAL4^sd-SG29.1

(Koelzer and Klein, 2006)

Detailed Description

Statement

Reference

Expression of H^Scer\UAS.cGa under the control of Scer\GAL4^ey.PH results in complete loss of the eye.

(Lee et al., 2009)

Expression of H^Scer\UAS.cGa throughout the wing disc, under the control of Scer\GAL4^sd-SG29.1, results in wing discs with no clear dorsal/ventral boundary.

(Koelzer and Klein, 2006)

Expression of H^Scer\UAS.cGa in non-sensory organ precursors, driven by Scer\GAL4^mα.hs, converts some of these cells into sensory organ precursors that are either normal or display a double shaft phenotype.

(Castro et al., 2005)

H^Scer\UAS.cGa; Scer\GAL4^ey.PH flies lack eyes.

(Chao et al., 2004)

Expression of H^Scer\UAS.cGa under the control of Scer\GAL4^sca-537.4 results in a bristle socket-to-shaft transformation phenotype. The converted socket cells and normal shaft cells produce shafts of nearly equal length. All bristles lack normal sockets and have ectopic shaft structures in flies expressing H^Scer\UAS.cGa under the control of Scer\GAL4^Su(H).ASE5.

(Barolo et al., 2002)

Embryos expressing H^Scer\UAS.cGa under the control of Scer\GAL4^byn-Gal4 lack hindgut boundary cells.

(Iwaki and Lengyel, 2002)

Expression of H^Scer\UAS.cGa under the control of Scer\GAL4^byn-Gal4 in embryos strongly suppresses boundary cell differentiation in the hindgut.

(Takashima et al., 2002)

Pupae in which H^Scer\UAS.cGa is expressed under the control of Scer\GAL4^hs.PB using heat shock at 37^oC for 2 hours during the tormogen-trichogen cell fate decision show socket-to-shaft cell fate transformations.

(Barolo et al., 2000)

Larvae expressing H^Scer\UAS.cGa under the control of Scer\GAL4^T113 have much smaller eye discs than normal. Adults derived from these larvae have small eyes, and in the most severe cases the eyes are missing. This eye phenotype is not associated with severe eye roughness. The flies also have small wings and halteres, and many extra thoracic bristles. Flies expressing H^Scer\UAS.cGa under the control of Scer\GAL4^vg.int2.1 have wing defects ranging from wing margin notches to rudimentary wings.

(Go et al., 1998)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

H^UAS.cGa, Scer\GAL4^ey.PH has visible phenotype, enhanceable by gro^AA.cLa.UAS, Scer\GAL4^ey.PH

(Lee et al., 2009)

H^UAS.cGa, Scer\GAL4^ey.PH has visible phenotype, enhanceable by gro^UAS.cAa, Scer\GAL4^ey.PH

(Lee et al., 2009)

H^UAS.cGa, Scer\GAL4^ey.PH has visible phenotype, enhanceable by ey^UAS.cHa, Scer\GAL4^ey.PH

(Lee et al., 2009)

Suppressed by

Statement

Reference

H^UAS.cGa, Scer\GAL4^ey.PH has visible phenotype, suppressible | partially by eyg^UAS.cUa, Scer\GAL4^ey.PH

(Lee et al., 2009)

H^UAS.cGa, Scer\GAL4^ey.PH has visible phenotype, suppressible | partially by H^UAS.cGa, Scer\GAL4^ey.PH

(Lee et al., 2009)

H^UAS.cGa, Scer\GAL4^ey.PH has visible phenotype, suppressible | partially by gro^EE.cLa.UAS, Scer\GAL4^ey.PH

(Lee et al., 2009)

H^UAS.cGa, Scer\GAL4^vg.int2.1 has visible phenotype, suppressible by N^ICN.UAS, Scer\GAL4^vg.int2.1

(Go et al., 1998)

Enhancer of

Statement

Reference

H^UAS.cGa, Scer\GAL4^bbg-C96 is an enhancer of visible phenotype of Scer\GAL4^bbg-C96, mam^N.UAS

(Helms et al., 1999)

Suppressor of

Statement

Reference

H^UAS.cGa, Scer\GAL4^ey.PH is a suppressor | partially of visible phenotype of H^UAS.cGa, Scer\GAL4^ey.PH

(Lee et al., 2009)

Phenotype Manifest In

Enhanced by

Statement

Reference

H^UAS.cGa, Scer\GAL4^ey.PH has eye phenotype, enhanceable by gro^AA.cLa.UAS, Scer\GAL4^ey.PH

(Lee et al., 2009)

H^UAS.cGa, Scer\GAL4^ey.PH has eye phenotype, enhanceable by gro^UAS.cAa, Scer\GAL4^ey.PH

(Lee et al., 2009)

H^UAS.cGa, Scer\GAL4^ey.PH has eye phenotype, enhanceable by ey^UAS.cHa, Scer\GAL4^ey.PH

(Lee et al., 2009)

Suppressed by

Statement

Reference

H^UAS.cGa, Scer\GAL4^ey.PH has eye phenotype, suppressible | partially by H^UAS.cGa, Scer\GAL4^ey.PH

(Lee et al., 2009)

H^UAS.cGa, Scer\GAL4^ey.PH has eye phenotype, suppressible | partially by gro^EE.cLa.UAS, Scer\GAL4^ey.PH

(Lee et al., 2009)

H^UAS.cGa, Scer\GAL4^ey.PH has eye phenotype, suppressible | partially by eyg^UAS.cUa, Scer\GAL4^ey.PH

(Lee et al., 2009)

H^UAS.cGa, Scer\GAL4^ey.PH has eye phenotype, suppressible | partially by eyg^UAS.lune, Scer\GAL4^ey.PH

(Chao et al., 2004)

H^UAS.cGa, Scer\GAL4^vg.int2.1 has wing phenotype, suppressible by N^ICN.UAS, Scer\GAL4^vg.int2.1

(Go et al., 1998)

Enhancer of

Statement

Reference

H^UAS.cGa, Scer\GAL4^bbg-C96 is an enhancer of wing margin bristle phenotype of Scer\GAL4^bbg-C96, mam^N.UAS

(Helms et al., 1999)

H^UAS.cGa, Scer\GAL4^bbg-C96 is an enhancer of wing phenotype of Scer\GAL4^bbg-C96, mam^N.UAS

(Helms et al., 1999)

Suppressor of

Statement

Reference

H^UAS.cGa, Scer\GAL4^ey.PH is a suppressor | partially of eye phenotype of H^UAS.cGa, Scer\GAL4^ey.PH

(Lee et al., 2009)

Other

Statement

Reference

H^UAS.cGa, Scer\GAL4^ey.PH, gro^AA.cLa.UAS has adult head phenotype

(Lee et al., 2009)

H^UAS.cGa, Scer\GAL4^ey.PH, gro^UAS.cAa has adult head phenotype

(Lee et al., 2009)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of hipk^{Scer\UAS.T:Ivir\HA1} partially suppresses the complete eye loss caused by expression of H^Scer\UAS.cGa under the control of Scer\GAL4^ey.PH; a small eye is seen.

Co-expression of gro^{EE.cLa.Scer\UAS} partially suppresses the complete eye loss caused by expression of H^Scer\UAS.cGa under the control of Scer\GAL4^ey.PH; a small eye is seen.

Co-expression of gro^{AA.cLa.Scer\UAS} enhances the phenotype caused by expression of H^Scer\UAS.cGa under the control of Scer\GAL4^ey.PH; the eye fails to develop, and head defects are increased, with the entire head being lost in 29% of flies and only the dorsal vertex of the head being present in 41% of the flies.

Co-expression of ey^Scer\UAS.cHa enhances the loss of eye phenotype caused by expression of H^Scer\UAS.cGa under the control of Scer\GAL4^ey.PH.

Co-expression of eyg^Scer\UAS.cUa suppresses the complete eye loss caused by expression of H^Scer\UAS.cGa under the control of Scer\GAL4^ey.PH.

(Lee et al., 2009)

Eye development in H^Scer\UAS.cGa; Scer\GAL4^ey.PH flies is partially rescued by eyg^{Scer\UAS.lune}.

(Chao et al., 2004)

Enhances the wing nicking phenotype seen in mam^N.Scer\UAS, Scer\GAL4^C96 flies.

(Helms et al., 1999)

Strong synergistic effects are seen in flies expressing H^Scer\UAS.cGa under the control of Scer\GAL4^T113 and also carrying Ser^Bd-3. The wing defects of flies expressing H^Scer\UAS.cGa under the control of Scer\GAL4^vg.int2.1 are largely suppressed if they are also carrying N^ICN.Scer\UAS.

(Go et al., 1998)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to rescue

H^UAS.cGa/Scer\GAL4^tin.CΔ4 fails to rescue Df(2L)Exel7007

(Kim et al., 2010)

Comments

Expression of Su(H)^Scer\UAS.cGa under the control of Scer\GAL4^tin.CΔ4 fails to rescue the abnormal cardiac function of Df(2L)Exel7007 flies.

(Kim et al., 2010)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

H^Scer\UAS.cGa

H^UAS.cGa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Go

(Go et al., 1998)

Secondary FlyBase IDs

References (15)

Report Sections

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