FlyBase Allele Report: Dmel\eyg[UAS.lune]

General Information

Symbol

Dmel\eyg^UAS.lune

Species

D. melanogaster

Name

FlyBase ID

FBal0150501

Feature type

allele

Associated gene

Dmel\eyg

Associated Insertion(s)

Carried in Construct

P{UAS-eyg.lune}

Also Known As

UAS-eyg

Key Links

Transgenic product class

cDNA

(Jang et al., 2003)

wild_type

(Jang et al., 2003)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Jang et al., 2003)

wild_type

(Jang et al., 2003)

Mutagen

in vitro construct

(Jang et al., 2003)

Progenitor genotype

Carried in construct

P{UAS-eyg.lune}

Cytology

Description

The 'lune' eyg cDNA is driven by UASt sequences.

(Jang et al., 2003)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

eyg

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

hyperplasia, with Scer\GAL4^dpp.blk1

(Chao et al., 2004)

Phenotype Manifest In

adult cuticle | ectopic, with Scer\GAL4^ey.PH

(Jang et al., 2003)

adult head | ventral, with Scer\GAL4^ey.PH

(Jang et al., 2003)

eye, with Scer\GAL4^ey.PH

(Chao et al., 2004)

eye | ectopic, with Scer\GAL4^ey.PH

(Jang et al., 2003)

eye | ectopic, with Scer\GAL4^upd1-E132

(Jang et al., 2003)

eye-antennal disc, with Scer\GAL4^dpp.blk1

(Chao et al., 2004)

eye-antennal disc, with Scer\GAL4^ey.PH

(Jang et al., 2003)

eye-antennal disc | proximal | somatic clone, with Scer\GAL4^Act5C.PI

(Chao et al., 2004)

wing disc | somatic clone | third instar larval stage, with Scer\GAL4^Act5C.PI

(Tanaka-Matakatsu et al., 2015)

Detailed Description

Statement

Reference

Clones expressing Scer\GAL4^Act5C.PI>eyg^{Scer\UAS.lune} are significantly larger than wild-type control clones.

(Tanaka-Matakatsu et al., 2015)

Overgrowth of the eye-antennal disc is seen in eyg^{Scer\UAS.lune}; Scer\GAL4^dpp.blk1 larvae, and in eyg^{Scer\UAS.lune}; Scer\GAL4^Act5C.PI somatic clones falling in proximal regions of the eye-antennal disc. eyg^{Scer\UAS.lune}; Scer\GAL4^ey.PH flies have enlarged eyes.

(Chao et al., 2004)

When eyg^{Scer\UAS.lune} is driven by Scer\GAL4^ey.PH results in ectopic eye formation. The ectopic eyes are almost always located ventral to the endogenous eye on the head. They often appear linked to the endogenous eye, but occasionally are well separated and are surrounded by orbital bristles. The penetrance of extra eye formation is low. Mutant flies also usually have some recognisable defect in the ventral head: the ventral posterior tim of the eye can be reduced and replaced by head cuticle, and/or a few extra bristles. Only 16% of animals have extra ommatidia and only 38% of these have the extra ommatidia as an isolated extra eye.

(Jang et al., 2003)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^dpp.blk1, eyg^UAS.lune has eye disc phenotype, enhanceable by ey^UAS.cHa/Scer\GAL4^dpp.blk1

(Jang et al., 2003)

Scer\GAL4^dpp.blk1, eyg^UAS.lune has eye | ectopic phenotype, enhanceable by ey^UAS.cHa/Scer\GAL4^dpp.blk1

(Jang et al., 2003)

NOT suppressed by

Statement

Reference

Scer\GAL4^upd1-E132, eyg^UAS.lune has eye | ectopic phenotype, non-suppressible by ey²

(Jang et al., 2003)

Enhancer of

Statement

Reference

Scer\GAL4^dpp.blk1/eyg^UAS.lune is an enhancer of eye disc phenotype of Scer\GAL4^dpp.blk1, ey^UAS.cHa

(Jang et al., 2003)

Scer\GAL4^dpp.blk1/eyg^UAS.lune is an enhancer of eye | ectopic phenotype of Scer\GAL4^dpp.blk1, ey^UAS.cHa

(Jang et al., 2003)

Suppressor of

Statement

Reference

eyg^UAS.lune, Scer\GAL4^ey.PH is a suppressor | partially of eye phenotype of N^ECN.UAS.cGa, Scer\GAL4^ey.PH

(Chao et al., 2004)

eyg^UAS.lune, Scer\GAL4^ey.PH is a suppressor | partially of eye phenotype of H^UAS.cGa, Scer\GAL4^ey.PH

(Chao et al., 2004)

Scer\GAL4^ey.PH/eyg^UAS.lune is a suppressor of eye phenotype of Scer\GAL4^ey.PH, fng^UAS.cKa

(Dominguez et al., 2004)

eyg^UAS.lune, Scer\GAL4^ey.PH is a suppressor of eye disc phenotype of N^ECN.UAS, Scer\GAL4^ey.PH

(Dominguez et al., 2004)

Scer\GAL4^dpp.blk1/eyg^UAS.lune is a suppressor | partially of eye phenotype of ey^D/ey²

(Jang et al., 2003)

NOT Suppressor of

Statement

Reference

Scer\GAL4^ey.PH/eyg^UAS.lune is a non-suppressor of eye phenotype of Df(1)os¹

(Chao et al., 2004)

Additional Comments

Genetic Interactions

Statement

Reference

Eye development in N^{ECN.Scer\UAS.cGa}; Scer\GAL4^ey.PH flies is partially rescued by eyg^{Scer\UAS.lune}. The resulting eyes are almost as big as wild-type. Eye development in H^Scer\UAS.cGa; Scer\GAL4^ey.PH flies is partially rescued by eyg^{Scer\UAS.lune}.

The small eye phenotype of Df(1)os¹ homozygotes is not suppressed by eyg^{Scer\UAS.lune}; Scer\GAL4^ey.PH.

(Chao et al., 2004)

Scer\GAL4^ey.PH-mediated expression of eyg^{Scer\UAS.lune} fully rescues the eye size defects caused by Scer\GAL4^ey.PH-mediated expression of fng^Scer\UAS.cKa and of N^ECN.Scer\UAS.

(Dominguez et al., 2004)

When ey^Scer\UAS.cHa and eyg^{Scer\UAS.lune} are driven by Scer\GAL4^dpp.blk1, the ectopic eyes produced are larger than ey^Scer\UAS.cHa or eyg^{Scer\UAS.lune} driven alone. This phenotype is more noticeable in the eye disc.

(Jang et al., 2003)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

Scer\GAL4^upd1-E132/eyg^UAS.lune rescues eyg¹/eyg^M3-12

(Jang et al., 2003)

Scer\GAL4^ey.PH/eyg^UAS.lune rescues eyg¹/eyg^M3-12

(Jang et al., 2003)

Partially rescues

Scer\GAL4^CD/eyg^UAS.lune partially rescues eyg^M3-12

(Wang et al., 2008)

Scer\GAL4^EX/eyg^UAS.lune partially rescues eyg^M3-12

(Wang et al., 2008)

Scer\GAL4^F1/eyg^UAS.lune partially rescues eyg^M3-12

(Wang et al., 2008)

Scer\GAL4^XS/eyg^UAS.lune partially rescues eyg^M3-12

(Wang et al., 2008)

Scer\GAL4^CD, Scer\GAL80^ts.αTub84B, eyg^UAS.lune partially rescues eyg^M3-12

(Wang et al., 2008)

Scer\GAL4^eyg.E2-EX/eyg^UAS.lune partially rescues eyg¹/eyg^M3-12

(Chao et al., 2004)

Scer\GAL4^eyg.E2-EX/eyg^UAS.lune partially rescues eyg^M3-12

(Chao et al., 2004)

Scer\GAL4^ey.PH/eyg^UAS.lune partially rescues eyg^123M11/eyg^PxA1

(Dominguez et al., 2004)

Comments

Expression of eyg^{Scer\UAS.lune} under the control of Scer\GAL4^CD rescues the loss of the eye-antennal disc and headless phenotype of the eyg^M3-12 mutant. In some cases, duplication of the larval eye-antennal disc and adult antenna occurs. The notum of these animals remains grooved.

Expression of eyg^{Scer\UAS.lune} under the control of Scer\GAL4^EX, Scer\GAL4^F1, or Scer\GAL4^XS partially rescues the eyg^M3-12 mutant. In all cases a small head with antennae but without eyes develops. The rescue efficiency of Scer\GAL4^XS (76%) and Scer\GAL4^EX (60%) are both much higher than Scer\GAL4^F1 (7%).

Expression of eyg^{Scer\UAS.lune} under the combined control of Scer\GAL4^EX and Scer\GAL4^XS partially rescues the eyg^M3-12 mutant to a slightly higher percentage (85%) than either driver alone. In this case the same level of rescue is observed, with flies having a small head with antennae but no eyes.

Expression of eyg^{Scer\UAS.lune} under the transient control of Scer\GAL4^CD during stage L2 (expression restricted by Scer\GAL80^ts.αTub84B throughout other stages of development) significantly rescues the eyg^M3-12 eye mutant phenotype. Earlier and later expression are not sufficient to rescue the eye development.

(Wang et al., 2008)

The loss of eyes in eyg^M3-12/eyg¹ and the lack of a head in eyg^M3-12 flies are both partially rescued by eyg^{Scer\UAS.lune}; Scer\GAL4^eyg.E2-EX.

(Chao et al., 2004)

One copy of eyg^{Scer\UAS.lune} under the control of Scer\GAL4^ey.PH rescues eyg^PxA1/eyg^123M11 mutants to healthy adulthood with almost normal-sized eyes.

(Dominguez et al., 2004)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

26809

w^*; P{UAS-eyg.lune}2

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

eyg^{Scer\UAS.lune}

eyg^UAS.lune

Name Synonyms

Secondary FlyBase IDs

References (8)

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