FlyBase Allele Report: Dmel\Egfr::tor[act.UAS]

General Information

Symbol

Dmel\Egfr::tor^act.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0093398

Feature type

allele

Associated gene

Egfr::tor

Associated Insertion(s)

Carried in Construct

P{UAS-tor⁴⁰²¹-EGFR}

Also Known As

UAS-Egfr^act, UAS-tor⁴⁰²¹-EGFR

Key Links

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Dominguez et al., 1998)

Progenitor genotype

Carried in construct

P{UAS-tor⁴⁰²¹-EGFR}

Cytology

Description

An activated form of Egfr is expressed under the control of UAS regulatory sequences.

(Dominguez et al., 1998)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Egfr::tor

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

adult gut, with Scer\GAL4^NP0001

(Buchon et al., 2010)

adult gut, with Scer\GAL4^NP0001, Scer\GAL80^ts.αTub84B

(Buchon et al., 2010)

dorsocentral bristle, with Scer\GAL4^C-765

(Mao and Freeman, 2009)

embryonic/larval brain | third instar larval stage, with Scer\GAL4^C855a

(Morante et al., 2013)

embryonic/larval ganglionic tracheal branch 1 | embryonic stage, with Scer\GAL4^bs-23.26

(Gallio et al., 2004)

embryonic segment, with Scer\GAL4^en-e16E

(Parker, 2006)

enterocyte | somatic clone, with Scer\GAL4^unspecified

(Buchon et al., 2010)

eye, with Scer\GAL4^hs.2sev

(Almudi et al., 2009)

eye disc, with Scer\GAL4^Act5C.PI

(Dominguez et al., 1998)

eye disc morphogenetic furrow, with Scer\GAL4^Act5C.PI

(Dominguez et al., 1998)

eye disc morphogenetic furrow | cell non-autonomous | ectopic | somatic clone, with Scer\GAL4^Act5C.PI

(Frankfort and Mardon, 2004)

eye disc morphogenetic furrow | somatic clone, with Scer\GAL4^Act5C.PI

(Brown et al., 2006)

larval neuroblast | ectopic | third instar larval stage, with Scer\GAL4^C855a

(Morante et al., 2013)

midgut, with Scer\GAL4^NP5130, Scer\GAL80^ts.αTub84B

(Buchon et al., 2010)

ommatidial precursor cluster | somatic clone, with Scer\GAL4^Act5C.PI

(Brown et al., 2006)

photoreceptor | cell autonomous | ectopic | somatic clone, with Scer\GAL4^Act5C.PI

(Frankfort and Mardon, 2004)

photoreceptor cell R7 | increased number, with Scer\GAL4^hs.2sev

(Almudi et al., 2009)

photoreceptor neuron, with Scer\GAL4^Act5C.PI

(Dominguez et al., 1998)

wing, with Scer\GAL4^Bx-MS1096

(Feldmann et al., 1999)

Detailed Description

Statement

Reference

Scer\GAL4^C855a-mediated expression of Egfr::tor^act.Scer\UAS results in overgrown larval L3 neuroepithelium and ectopic neuroblasts.

(Morante et al., 2013)

Overexpression of Egfr::tor^act.Scer\UAS in intestinal stem cells (ISCs) under the control of Scer\GAL4^esg-NP5130 and Scer\GAL80^ts.αTub84B is sufficient to induce proliferation along the gut in the absence of infection, although the resulting cells are abnormally shaped and do not appear as fully differentiated enterocytes.

Expression of Egfr::tor^act.Scer\UAS in enterocytes under the control of Scer\GAL4^{Myo31DF-NP0001} results in a adult flies with guts two times shorter and three times wider than wild-type fly guts.

Guts of flies overexpressing Egfr::tor^act.Scer\UAS under the control of Scer\GAL4^{Myo31DF-NP0001} and Scer\GAL80^ts.αTub84B are shorter compared with guts wild-type flies 48 hours post-infection with Erwinia carotovora carotovora 15 (Ecc15).

Guts of flies expressing Egfr::tor^act.Scer\UAS in enterocytes under the control of Scer\GAL4^{Myo31DF-NP0001} and Scer\GAL80^ts.αTub84B do not elongate upon infection with Erwinia carotovora carotovora 15 (Ecc15). After a recovery phase, the guts are 40% shorter than their wild-type counterparts.

Overexpression of Egfr::tor^act.Scer\UAS in enterocytes under the control of Scer\GAL4^{Myo31DF-NP0001} and Scer\GAL80^ts.αTub84B induces hight levels of cell delamination in the absence of Erwinia carotovora carotovora 15 (Ecc15) infection.

Clones of enterocytes overexpressing Egfr::tor^act.Scer\UAS under the control of Scer\GAL4^{Myo31DF-NP0001} and Scer\GAL80^ts.αTub84B rapidly disappear from the epithelium compared with wild-type clones suggesting an increased rate of delamination. The detached cells in these guts differ from those in wild-type flies in that they do not undergo cell death, have normal nuclei and do not contain enlarged vacuoles.

(Buchon et al., 2010)

Expression of Egfr::tor^act.Scer\UAS under the control of Scer\GAL4^hs.2sev results in a rough eye phenotype. 20% of ommatidia have an extra R7 photoreceptor cell.

(Almudi et al., 2009)

Expression of Egfr::tor^act.Scer\UAS in the adult notum under the control of Scer\GAL4^C-765 results in extra dorsocentral bristles.

(Mao and Freeman, 2009)

Clones that express Egfr::tor^act.Scer\UAS under the control of Scer\GAL4^Act5C.PI form large, disorganised clusters in morphogenetic furrow. The cells of the furrow appear to be disrupted in these clones.

(Brown et al., 2006)

Expression of Egfr::tor^act.Scer\UAS, under the control of Scer\GAL4^en-e16E, in the posterior compartment cells if embryos leads to a reduction in the level of apoptosis of these cells, but has no effect on mitosis. Cells are of an increased size leading to an increase in compartment size of 18%.

(Parker, 2006)

In late 3rd instar eye discs, Egfr::tor^act.Scer\UAS; Scer\GAL4^Act5C.PI somatic clones exhibit ectopic neuronal differentiation, but photoreceptors in these clones fail to express R8 markers. When the clones fall anterior to the morphogenetic furrow, precocious neural differentiation occurs within the clone which also non-autonomously induces the formation of ectopic furrows.

(Frankfort and Mardon, 2004)

Ganglionic branch 1s turn prematurely before reaching the midline in Egfr::tor^act.Scer\UAS; Scer\GAL4^bs-23.26 embryos.

(Gallio et al., 2004)

Clones of Egfr::tor^act.Scer\UAS (driven by Scer\GAL4^αTub84B.PC) in the adult abdomen do not show any consistent alterations of normal polarity.

(Lawrence et al., 2002)

Expression of Egfr::tor^act.Scer\UAS under the control of Scer\GAL4^btl.PS does not confer dorsal trunk or visceral branch features to other tracheal branches.

(Llimargas, 2000)

Flies expressing Egfr::tor^act.Scer\UAS under the control of Scer\GAL4^Bx-MS1096 do not eclose at 25^oC, at 18^oC the flies eclose but have abnormal wings.

(Feldmann et al., 1999)

Clones in the eye disc expressing Egfr::tor^act.Scer\UAS under the control of Scer\GAL4^Act5C.PI posterior to the morphogenetic furrow show extra photoreceptor cell recruitment. Ectopic morphogenetic furrow formation is seen.

(Dominguez et al., 1998)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Egfr::tor^act.UAS, Scer\GAL4^NP5130 has increased occurrence of cell division | adult stage phenotype, suppressible | partially by dome^ΔTMCYT.UAS, Scer\GAL4^NP5130

(Buchon et al., 2010)

Egfr::tor^act.UAS, Scer\GAL4^NP5130 has increased occurrence of cell division | adult stage phenotype, suppressible | partially by Socs36E^UAS.cCa, Scer\GAL4^NP5130

(Buchon et al., 2010)

Egfr::tor^act.UAS, Scer\GAL4^NP5130 has increased occurrence of cell division | adult stage phenotype, suppressible | partially by Stat92E^NIG.cUa, Scer\GAL4^NP5130

(Buchon et al., 2010)

NOT suppressed by

Statement

Reference

Egfr::tor^act.UAS, Scer\GAL4^hs.2sev has visible phenotype, non-suppressible by Socs36E^UASp.cCa, Scer\GAL4^hs.2sev

(Almudi et al., 2009)

Egfr::tor^act.UAS has abnormal size phenotype, non-suppressible by Scer\GAL4^en-e16E/CycE^UAS.cLa

(Parker, 2006)

Suppressor of

Statement

Reference

Egfr::tor^act.UAS, Scer\GAL4^en-e16E is a suppressor of increased cell death | embryonic stage phenotype of CycE^UAS.cLa, Scer\GAL4^en-e16E

(Parker, 2006)

Phenotype Manifest In

Suppressed by

Statement

Reference

Egfr::tor^act.UAS, Scer\GAL4^NP5130, Scer\GAL80^ts.αTub84B has midgut phenotype, suppressible | partially by Socs36E^UAS.cCa, Scer\GAL4^NP5130, Scer\GAL80^ts.αTub84B

(Buchon et al., 2010)

Egfr::tor^act.UAS, Scer\GAL4^NP5130, Scer\GAL80^ts.αTub84B has midgut phenotype, suppressible | partially by Stat92E^NIG.cUa, Scer\GAL4^NP5130, Scer\GAL80^ts.αTub84B

(Buchon et al., 2010)

Egfr::tor^act.UAS, Scer\GAL4^NP5130, Scer\GAL80^ts.αTub84B has midgut phenotype, suppressible | partially by dome^ΔTMCYT.UAS, Scer\GAL4^NP5130, Scer\GAL80^ts.αTub84B

(Buchon et al., 2010)

Egfr::tor^act.UAS, Scer\GAL4^Act5C.PI has eye disc morphogenetic furrow | ectopic | somatic clone | cell non-autonomous phenotype, suppressible by sens^UAS.cNa, Scer\GAL4^Act5C.PI

(Frankfort and Mardon, 2004)

Egfr::tor^act.UAS, Scer\GAL4^Act5C.PI has photoreceptor | ectopic | somatic clone | cell autonomous phenotype, suppressible | partially by sens^UAS.cNa, Scer\GAL4^Act5C.PI

(Frankfort and Mardon, 2004)

NOT suppressed by

Statement

Reference

Egfr::tor^act.UAS, Scer\GAL4^hs.2sev has eye phenotype, non-suppressible by Socs36E^UASp.cCa, Scer\GAL4^hs.2sev

(Almudi et al., 2009)

Egfr::tor^act.UAS, Scer\GAL4^hs.2sev has photoreceptor cell R7 | increased number phenotype, non-suppressible by Socs36E^UASp.cCa, Scer\GAL4^hs.2sev

(Almudi et al., 2009)

Suppressor of

Statement

Reference

Egfr::tor^act.UAS/Scer\GAL4^GMR.PF is a suppressor of ommatidial precursor cluster | somatic clone phenotype of ato¹

(Brown et al., 2006)

Egfr::tor^act.UAS, Scer\GAL4^en-e16E is a suppressor of embryonic segment posterior compartment phenotype of CycE^UAS.cLa, Scer\GAL4^en-e16E

(Parker, 2006)

Scer\GAL4^insc-Mz1407/Egfr::tor^act.UAS is a suppressor of embryonic central brain phenotype of sim²

(Page, 2003)

NOT Suppressor of

Statement

Reference

Egfr::tor^act.UAS/Scer\GAL4^αTub84B.PL is a non-suppressor of ommatidial precursor cluster | somatic clone phenotype of pnt^Δ88

(Brown et al., 2006)

Other

Statement

Reference

ato³/Df(3R)p13, Egfr::tor^act.UAS, Scer\GAL4^bi-omb-Gal4 has eye disc & neuron phenotype

(Dominguez et al., 1998)

Egfr::tor^act.UAS, Scer\GAL4^bi-omb-Gal4, ato³ has eye disc & neuron phenotype

(Dominguez et al., 1998)

Additional Comments

Genetic Interactions

Statement

Reference

The mitotic index in the gut of flies overexpressing Egfr::tor^act.Scer\UAS is decreased but not abolished when dome^{ΔTMCYT.Scer\UAS} is co-expressed under the control of Scer\GAL4^esg-NP5130 and Scer\GAL80^ts.αTub84B.

The mitotic index in the gut of flies overexpressing Egfr::tor^act.Scer\UAS is decreased but not abolished when Stat92E^NIG.cUa is co-expressed under the control of Scer\GAL4^esg-NP5130 and Scer\GAL80^ts.αTub84B.

The mitotic index in the gut of flies overexpressing Egfr::tor^act.Scer\UAS is decreased but not abolished when Socs36E^Scer\UAS.cCa is co-expressed under the control of Scer\GAL4^esg-NP5130 and Scer\GAL80^ts.αTub84B.

(Buchon et al., 2010)

The rough eye and extra R7 photoreceptor cell phenotypes caused by expression of Egfr::tor^act.Scer\UAS under the control of Scer\GAL4^hs.2sev are not suppressed by co-expression of Socs36E^{Scer\UAS.P\T.cCa}.

(Almudi et al., 2009)

Expression of Egfr::tor^act.Scer\UAS under the control of Scer\GAL4^αTub84B.PL does not rescue the lack of arcs and rosettes in the morphogenetic furrow of eye discs with pnt^Δ88 Minute clones.

Expression of Egfr::tor^act.Scer\UAS under the control of Scer\GAL4^GMR.PF partially rescues the lack of cluster formation in the morphogenetic furrow of ato¹ eye disc clones.

(Brown et al., 2006)

Coexpression of Egfr::tor^act.Scer\UAS and CycE^Scer\UAS.cLa, under the control of Scer\GAL4^en-e16E, blocks CycE^Scer\UAS.cLa-induced apoptosis in the embryonic posterior compartment cells. There is a reduction in compartment size, similar to that seen when only Ras85D^V12.Scer\UAS is expressed, and cells are of a size similar to that seen when only CycE^Scer\UAS.cLa is expressed.

(Parker, 2006)

Precocious and ectopic neuronal differentiation in Egfr::tor^act.Scer\UAS; Scer\GAL4^Act5C.PI somatic clones in the eye discs is largely suppressed by sens^Scer\UAS.cNa, as is ectopic morphogenetic furrow formation.

(Frankfort and Mardon, 2004)

The addition of Egfr::tor^act.Scer\UAS (driven by Scer\GAL4¹⁴⁰⁷) to sim² suppresses the sim² embryonic brain phenotype. In 55% of embryos, restoration of the midbrain, including ganglia and axons tracts is seen.

(Page, 2003)

Neuronal differentiation is seen in ato³/Df(3R)p13 eye discs expressing Egfr::tor^act.Scer\UAS under the control of Scer\GAL4^bi-omb-Gal4.

(Dominguez et al., 1998)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Egfr::tor^act.Scer\UAS

Egfr::tor^act.UAS

Name Synonyms

Activated Egfr

(Lawrence et al., 2002)

Secondary FlyBase IDs

References (20)

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