FlyBase Allele Report: Dmel\Bx[EP1306]

General Information

Symbol

Dmel\Bx^EP1306

Species

D. melanogaster

Name

FlyBase ID

FBal0093492

Feature type

allele

Associated gene

Dmel\Bx

Associated Insertion(s)

P{EP}Bx^EP1306

Carried in Construct

Also Known As

EP1306, UAS-dLMO

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(Milan and Cohen, 2003, Pena-Rangel et al., 2002, Gene Disruption Project members, 2001-, Abdelilah-Seyfried et al., 2000, Milan and Cohen, 1999, Milan et al., 1998, Zeng et al., 1998)

Progenitor genotype

Associated Insertion(s)

P{EP}Bx^EP1306

Cytology

Description

Insertion of a P{EP} element 235bp upstream of the 5' end of exon 1a. The P{EP} element is inserted so that a sense Bx transcript is produced by transcription from the promoter in the P{EP} element.

(Milan et al., 1998)

Insertion of a P{EP} element upstream of the 5' end of the Bxa transcript.

(Zeng et al., 1998)

Allele components

Component

Use(s)

Inserted element

P{EP}

misexpression element

Regulatory region(s)

UAS

binary expression system - regulatory region

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

X:18,534,638..18,534,638 [+]

(Lasek et al., 2011, Asmar et al., 2008, Hari et al., 2008, Tsai et al., 2004, Milan and Cohen, 2003, Pena-Rangel et al., 2002, Gene Disruption Project members, 2001-, Abdelilah-Seyfried et al., 2000, Milan and Cohen, 1999, Milan et al., 1998, Zeng et al., 1998)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

AJ010389

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

dorsocentral bristle, with Scer\GAL4^sca-537.4

(Abdelilah-Seyfried et al., 2000)

scutellar bristle, with Scer\GAL4^sca-537.4

(Abdelilah-Seyfried et al., 2000)

scutum, with Scer\GAL4^ptc-559.1

(Zeng et al., 1998)

wing, with Scer\GAL4^ap-md544

(Milan and Cohen, 1999)

wing, with Scer\GAL4^CCAP.PP

(Hari et al., 2008)

wing, with Scer\GAL4^CCAP.PP, Scer\GAL80^ts.αTub84B

(Hari et al., 2008)

wing, with Scer\GAL4^ptc-559.1

(Zeng et al., 1998)

wing | dorsal, with Scer\GAL4^bi-omb-Gal4

(Milan et al., 1998)

wing | ectopic, with Scer\GAL4^ptc-559.1

(Zeng et al., 1998)

wing disc | somatic clone, with Scer\GAL4^Act5C.PP

(Milan and Cohen, 2003)

wing margin, with Scer\GAL4^ap-md544

(Milan and Cohen, 1999)

wing margin, with Scer\GAL4^bi-omb-Gal4

(Milan et al., 1998)

wing margin | ectopic, with Scer\GAL4^ap-md544

(Milan and Cohen, 1999)

wing margin | ectopic, with Scer\GAL4^bi-omb-Gal4

(Milan et al., 1998)

Detailed Description

Statement

Reference

Mutant flies show increased sensitivity to ethanol sedation compared to control flies.

(Lasek et al., 2011)

Expression of Bx^EP1306 under the control of Scer\GAL4^Ccap.PP gives rise to flies that fail to expand their wings after eclosion.

Mis-expression of Bx^EP1306 under the control of Scer\GAL4^Ccap.PP for the entire pupal period (using Scer\GAL80^ts.αTub84B at 25[o]C to block expression up to the pupal period) leads to a fully penetrant wing expansion defect. Mis-expression 40 hours after pupal formation, however, does not affect post-eclosion behavior.

(Hari et al., 2008)

Mutant animals exhibit an increased sensitivity to cocaine. Mutants exposed to 75ug of cocaine behave similarly to control flies exposed to 100ug of the drug. The number and detailed morphology of LN_v neurons is normal in mutant animals. Mutant adults exhibit a less robust locomotor rhythm in constant darkness than control flies.

(Tsai et al., 2004)

When clones are made in the wing disc that express Bx^EP1306 (driven by Scer\GAL4^Act5C.PP), they are round in shape with smooth borders and sort into the ventral compartment. When clones are made in the wing disc expressing Bx^EP1306, they do not respect the dorsal ventral boundary.

(Milan and Cohen, 2003)

Expression of Bx^EP1306 under the control of Scer\GAL4^sca-537.4 results in the loss of several scutellar and dorsocentral macrochaetae.

(Abdelilah-Seyfried et al., 2000)

Flies expressing Bx^EP1306 under the control of Scer\GAL4^ap-md544 show loss of the normal wing margin and sporadic patches of ectopic wing margin, which promote local overgrowth.

(Milan and Cohen, 1999)

Flies expressing Bx^EP1306 under the control of Scer\GAL4^bi-omb-Gal4 lack most of the normal wing margin and have irregular pieces of ectopic margin associated with overgrowths in the dorsal surface of the wing.

(Milan et al., 1998)

Flies expressing Bx^EP1306 under the control of Scer\GAL4^ptc-559.1 show ectopic wing formation on the wing or notum.

(Zeng et al., 1998)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Bx^EP1306, Scer\GAL4^CCAP.PP has abnormal eclosion phenotype, suppressible by Chi^{FL.UAS.Tag:MYC}, Scer\GAL4^CCAP.PP

(Hari et al., 2008)

Bx^EP1306, Scer\GAL4^CCAP.PP has abnormal eclosion phenotype, suppressible by tup^UAS.cTa, Scer\GAL4^CCAP.PP

(Hari et al., 2008)

NOT suppressed by

Statement

Reference

Bx^EP1306, Scer\GAL4^CCAP.PP has abnormal eclosion phenotype, non-suppressible by dimm^UAS.Tag:MYC, Scer\GAL4^CCAP.PP

(Hari et al., 2008)

Bx^EP1306, Scer\GAL4^CCAP.PP has abnormal eclosion phenotype, non-suppressible by Lim3^UAS.cTa, Scer\GAL4^CCAP.PP

(Hari et al., 2008)

Enhancer of

Statement

Reference

Bx^EP1306/Scer\GAL4^pnr-MD237 is an enhancer of visible phenotype of pnr^MD237

(Pena-Rangel et al., 2002)

Other

Statement

Reference

Bx^EP1306, Scer\GAL4^ap-md544, ap::Chi^ChAp.UAS has visible phenotype

(Milan and Cohen, 1999)

Phenotype Manifest In

Suppressed by

Statement

Reference

Bx^EP1306, Scer\GAL4^CCAP.PP has wing phenotype, suppressible by Chi^{FL.UAS.Tag:MYC}, Scer\GAL4^CCAP.PP

(Hari et al., 2008)

Bx^EP1306, Scer\GAL4^CCAP.PP has wing phenotype, suppressible by tup^UAS.cTa, Scer\GAL4^CCAP.PP

(Hari et al., 2008)

Bx^EP1306, Scer\GAL4^Act5C.PP has wing disc | somatic clone phenotype, suppressible | partially by N^ECN.UAS.cGa, Scer\GAL4^Act5C.PP

(Milan and Cohen, 2003)

Bx^EP1306, Scer\GAL4^Act5C.PP has wing disc | somatic clone phenotype, suppressible by fng^{UAS.B.Tag:MYC}, Scer\GAL4^Act5C.PP

(Milan and Cohen, 2003)

NOT suppressed by

Statement

Reference

Bx^EP1306, Scer\GAL4^CCAP.PP has wing phenotype, non-suppressible by dimm^UAS.Tag:MYC, Scer\GAL4^CCAP.PP

(Hari et al., 2008)

Bx^EP1306, Scer\GAL4^CCAP.PP has wing phenotype, non-suppressible by Lim3^UAS.cTa, Scer\GAL4^CCAP.PP

(Hari et al., 2008)

Bx^EP1306, Scer\GAL4^ap-md544 has wing margin phenotype, non-suppressible by ap^UAS.cMa, Scer\GAL4^ap-md544

(Milan and Cohen, 1999)

Enhancer of

Statement

Reference

Bx^EP1306/Scer\GAL4^pnr-MD237 is an enhancer of chaeta phenotype of pnr^MD237

(Pena-Rangel et al., 2002)

Suppressor of

Statement

Reference

Bx^EP1306, Scer\GAL4^Act5C.PP is a suppressor of wing disc | somatic clone phenotype of Scer\GAL4^Act5C.PP, fng^{UAS.B.Tag:MYC}

(Milan and Cohen, 2003)

Other

Statement

Reference

Bx^EP1306, Scer\GAL4^pnr-MD237, pnr^MD237/pnr[+] has scutellar bristle | increased number phenotype

(Pena-Rangel et al., 2002)

Bx^EP1306, Scer\GAL4^pnr-MD237, pnr^MD237 has scutellar bristle | increased number phenotype

(Pena-Rangel et al., 2002)

Bx^EP1306, Scer\GAL4^ap-md544, ap::Chi^ChAp.UAS has wing phenotype

(Milan and Cohen, 1999)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of Chi^{FL.Scer\UAS.T:Hsap\MYC} with Bx^EP1306, both under the control of Scer\GAL4^Ccap.PP, suppresses the wing expansion defect found upon expression of Bx^EP1306 alone.

Expression of tup^Scer\UAS.cTa with Bx^EP1306, both under the control of Scer\GAL4^Ccap.PP, suppresses the wing expansion defect found upon expression of Bx^EP1306 alone.

Expression of dimm^{Scer\UAS.T:Hsap\MYC} with Bx^EP1306, both under the control of Scer\GAL4^Ccap.PP, fails to suppress the wing expansion defect found upon expression of Bx^EP1306 alone.

Expression of Lim3^Scer\UAS.cTa with Bx^EP1306, both under the control of Scer\GAL4^Ccap.PP, fails to suppress the wing expansion defect found upon expression of Bx^EP1306 alone.

(Hari et al., 2008)

When clones are made expressing both Bx^EP1306 and N^{ECN.Scer\UAS.cGa} or fng^{Scer\UAS.B.T:Hsap\MYC} are made some of them are round in shape, others more irregular and do not sort into the ventral compartment.

(Milan and Cohen, 2003)

The phenotype of flies expressing Bx^EP1306 under the control of Scer\GAL4^ap-md544 is not suppressed by coexpression of ap^Scer\UAS.cMa. Flies coexpressing Bx^EP1306 and ap::Chi^{ChAp.Scer\UAS} under the control of Scer\GAL4^ap-md544 have a normal wing margin and resemble the Bx^- mutant wing.

(Milan and Cohen, 1999)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Bx^EP1306 is rescued by Bx^EP1306/Scer\GAL4^P2.4.Pdf

(Tsai et al., 2004)

Bx^EP1306 is rescued by Bx^EP1306/Scer\GAL4^hs.PB

(Tsai et al., 2004)

Rescues

Bx^EP1306/Scer\GAL4^P2.4.Pdf rescues Bx^EP1306

(Tsai et al., 2004)

Bx^EP1306/Scer\GAL4^hs.PB rescues Bx^EP1306

(Tsai et al., 2004)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Selected as: a P{EP} insertion line that modifies the pnr^MD237/+ phenotype when expressed using Scer\GAL4^pnr-MD237.

(Pena-Rangel et al., 2002)

Comments

The cocaine sensitivity phenotype is revertable by excision of the P{EP} insertion.

(Tsai et al., 2004)

External Crossreferences and Linkouts ( 1 )

Crossreferences

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

AJ010389

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

Bx^EP1306

EP(X)1306

(Abdelilah-Seyfried et al., 2000)

EP1306

(Milan et al., 1998, Zeng et al., 1998)

hdp^EP1306

(Asmar et al., 2008)

Name Synonyms

Secondary FlyBase IDs

References (12)

Report Sections

Open Close