FlyBase Allele Report: Dmel\sli[UAS.cBa]

General Information

Symbol

Dmel\sli^UAS.cBa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Battye

FlyBase ID

FBal0096689

Feature type

allele

Associated gene

Dmel\sli

Associated Insertion(s)

Carried in Construct

P{UAS-sli.B}

Also Known As

UAS-slit

Key Links

Transgenic product class

cDNA

(Battye et al., 1999)

wild_type

(Battye et al., 1999)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Battye et al., 1999)

wild_type

(Battye et al., 1999)

Mutagen

in vitro construct

(Battye et al., 1999)

Progenitor genotype

Carried in construct

P{UAS-sli.B}

Cytology

Description

Expression of 4.2kb sli cDNA is governed by UASt regulatory sequences.

(Battye et al., 1999)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

sli

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates Alzheimer's disease

modeled by Hsap\MAPT^V337M.UAS

(Shulman et al., 2011)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

axon, with Scer\GAL4^en-e16E

(Battye et al., 1999)

axon, with Scer\GAL4^sim.P3.7

(Battye et al., 1999)

axon, with Scer\GAL4^sli.PS

(Battye et al., 1999)

cardioblast, with Scer\GAL4^eve.eme

(Qian et al., 2005)

cardioblast, with Scer\GAL4^how-24B, Scer\GAL4^twi.PG

(Qian et al., 2005)

central nervous system, with Scer\GAL4^sim.P3.7

(Battye et al., 1999)

central nervous system, with Scer\GAL4^sli.PS

(Battye et al., 1999)

fascicle, with Scer\GAL4^sca-537.4

(Battye et al., 1999)

larval longitudinal connective, with Scer\GAL4^en-e16E

(Battye et al., 1999)

larval longitudinal connective, with Scer\GAL4^sca-537.4

(Battye et al., 1999)

larval longitudinal connective, with Scer\GAL4^sim.P3.7

(Battye et al., 1999)

larval longitudinal connective, with Scer\GAL4^sli.PS

(Battye et al., 1999)

larval ventral nerve cord, with Scer\GAL4^elav-C155

(Battye et al., 2001)

larval ventral nerve cord, with Scer\GAL4^en-e16E

(Battye et al., 2001)

larval ventral nerve cord, with Scer\GAL4^sli.PS

(Battye et al., 2001)

MP1 neuron & fascicle, with Scer\GAL4^en-e16E

(Battye et al., 1999)

SP1 neuron, with Scer\GAL4^sim.P3.7

(Battye et al., 1999)

SP1 neuron & fascicle, with Scer\GAL4^en-e16E

(Battye et al., 1999)

symmetrical commissure, with Scer\GAL4^sim.P3.7

(Battye et al., 1999)

symmetrical commissure, with Scer\GAL4^sli.PS

(Battye et al., 1999)

ventral nerve cord, with Scer\GAL4^sim.P3.7

(Battye et al., 1999)

ventral nerve cord, with Scer\GAL4^sli.PS

(Battye et al., 1999)

vMP2 neuron & fascicle, with Scer\GAL4^en-e16E

(Battye et al., 1999)

Detailed Description

Statement

Reference

Expression of sli^UAS.cBa under the control of Scer\GAL4^ey-OK107 does not lead to any changes in the development of the optic lobes when compared to controls.

(Caipo et al., 2020)

Expression of sli^Scer\UAS.cBa in myocardial cells, driven by Scer\GAL4^tin.CΔ4, does not produce significant cardiac alignment defects. Pan-mesodermal expression of this transgene, driven by both Scer\GAL4^how-24B and Scer\GAL4^twi.PG, produces cardiac alignment defects with low penetrance. In contrast, sli^Scer\UAS.cBa expression in pericardial cells, driven by Scer\GAL4^eve.eme, results in frequent alignment defects.

(Qian et al., 2005)

Embryos expressing sli^Scer\UAS.cBa under the control of Scer\GAL4^tracheal generally have normal dorsal sensory neuron morphology.

(Parsons et al., 2003)

When expression of sli^Scer\UAS.cBa is driven by Scer\GAL4^sli.PS, Scer\GAL4^elav-C155 or Scer\GAL4^en-e16E the ventral nerve cord is disrupted with interruption of intersegmental connections and displacement of the axon tracts away from the source of sli protein.

(Battye et al., 2001)

When expression is driven by Scer\GAL4^sim.P3.7 in an otherwise wild type background, fewer than the normal number of axons cross the midline. Longitudinal tracts are thicker within segments and thinner between segments than normal. The position of the SP1 neuron is irregular. When expression is driven by Scer\GAL4^sli.PS, defasciculation of commissural tracts occurs, some commissures are missing and ectopic lateral axon extension occurs. Expression driven by Scer\GAL4^en-e16E results in breaks in the longitudinal tracts, including the MP fascicle and the later-forming SP1 fascicle. Axons inappropriately cross the midline. Expression driven by Scer\GAL4^sca-537.4 results in a reduction in the number of axons traversing the segment boundary in the longitudinal tracts, thinning and delaying development of fascicles.

(Battye et al., 1999)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

sli^UAS.cBa, Scer\GAL4^GMR.PU is a suppressor of visible | adult stage phenotype of Hsap\MAPT^V337M.UAS, Scer\GAL4^GMR.PU

(Shulman et al., 2011)

Phenotype Manifest In

Suppressor of

Statement

Reference

sli^UAS.cBa, Scer\GAL4^GMR.PU is a suppressor of eye | adult stage phenotype of Hsap\MAPT^V337M.UAS, Scer\GAL4^GMR.PU

(Shulman et al., 2011)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

The moderate rough eye phenotype and reduced adult eye size characteristic for flies expressing Scer\GAL4^GMR.PU under the control of Hsap\MAPT^{V337M.Scer\UAS} is suppressed by co-expression of sli^Scer\UAS.cBa , restoring a near wild-type phenotype.

(Shulman et al., 2011)

Complementation and Rescue Data

Rescues

Scer\GAL4^ey-OK107/sli^UAS.cBa rescues sli²/sli^k04807b

(Caipo et al., 2020)

Partially rescues

Scer\GAL4^GMR.long/sli^UAS.cBa partially rescues sli²/sli^k04807b

(Caipo et al., 2020)

Scer\GAL4^repo/sli^UAS.cBa partially rescues sli²/sli^k04807b

(Caipo et al., 2020)

Scer\GAL4^B2-3-30/sli^UAS.cBa partially rescues sli²

(MacMullin and Jacobs, 2006)