FlyBase Allele Report: Dmel\S[UAS.cGb]

General Information

Symbol

Dmel\S^UAS.cGb

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct b of Guichard

FlyBase ID

FBal0098828

Feature type

allele

Associated gene

Dmel\S

Associated Insertion(s)

Carried in Construct

P{UAS-Star.G}

Also Known As

UAS-Star

Key Links

Transgenic product class

cDNA

(Guichard et al., 1999)

wild_type

(Guichard et al., 1999)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Guichard et al., 1999)

wild_type

(Guichard et al., 1999)

Mutagen

in vitro construct

(Guichard et al., 1999)

Progenitor genotype

Carried in construct

P{UAS-Star.G}

Cytology

Description

The full length cDNA is expressed under the control of UASt regulatory sequences.

(Guichard et al., 1999)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

visible, with Scer\GAL4^Bx-MS1096

(Guichard et al., 1999)

Phenotype Manifest In

wing, with Scer\GAL4^Bx-MS1096

(Guichard et al., 1999)

wing vein | ectopic, with Scer\GAL4^Bx-MS1096

(Guichard et al., 2002)

Detailed Description

Statement

Reference

S^Scer\UAS.cGb; Scer\GAL4^Bx-MS1096 have faint ectopic vein material near longitudinal veins.

(Guichard et al., 2002)

Expression of S^Scer\UAS.cGb when driven by Scer\GAL4^Bx-MS1096 has no detectable effect.

(Guichard et al., 2000)

Strong ectopic expression of S^Scer\UAS.cGb directed by Scer\GAL4^Bx-MS1096 induces faint extravein material located near longitudinal veins.

(Guichard et al., 1999)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhanced by

Statement

Reference

S^UAS.cGb, Scer\GAL4^Bx-MS1096 has visible phenotype, non-enhanceable by spi^hs.PG

(Guichard et al., 1999)

NOT suppressed by

Statement

Reference

S^UAS.cGb, Scer\GAL4^Bx-MS1096 has visible phenotype, non-suppressible by spi^hs.PG

(Guichard et al., 1999)

NOT Enhancer of

Statement

Reference

rho^UAS.cBa/S^UAS.cGb, Scer\GAL4^Bx-MS1096 is a non-enhancer of visible phenotype of Egfr^DN.UAS.cBa, Scer\GAL4^Bx-MS1096

(Guichard et al., 1999)

NOT Suppressor of

Statement

Reference

rho^UAS.cBa/S^UAS.cGb, Scer\GAL4^Bx-MS1096 is a non-suppressor of visible phenotype of Egfr^DN.UAS.cBa, Scer\GAL4^Bx-MS1096

(Guichard et al., 1999)

Other

Statement

Reference

S^UAS.cGb, Scer\GAL4^kni.L2, rho^UAS.cBa has visible phenotype

(Lunde et al., 2003)

Phenotype Manifest In

Enhancer of

Statement

Reference

S^UAS.cGb, Scer\GAL4^Bx-MS1096 is an enhancer of wing vein | ectopic phenotype of Scer\GAL4^Bx-MS1096, stet^UAS.cGa

(Guichard et al., 2000)

NOT Enhancer of

Statement

Reference

S^UAS.cGb, Scer\GAL4^Bx-MS1096 is a non-enhancer of wing phenotype of Egfr^DN.UAS.cBa, Scer\GAL4^Bx-MS1096, rho^UAS.cBa

(Guichard et al., 1999)

S^UAS.cGb, Scer\GAL4^Bx-MS1096 is a non-enhancer of wing vein phenotype of Egfr^DN.UAS.cBa, Scer\GAL4^Bx-MS1096, rho^UAS.cBa

(Guichard et al., 1999)

S^UAS.cGb, Scer\GAL4^Bx-MS1096 is a non-enhancer of wing vein L3 phenotype of Egfr^DN.UAS.cBa, Scer\GAL4^Bx-MS1096, rho^UAS.cBa

(Guichard et al., 1999)

S^UAS.cGb, Scer\GAL4^Bx-MS1096 is a non-enhancer of wing vein L4 phenotype of Egfr^DN.UAS.cBa, Scer\GAL4^Bx-MS1096, rho^UAS.cBa

(Guichard et al., 1999)

S^UAS.cGb, Scer\GAL4^Bx-MS1096 is a non-enhancer of wing vein L5 phenotype of Egfr^DN.UAS.cBa, Scer\GAL4^Bx-MS1096, rho^UAS.cBa

(Guichard et al., 1999)

NOT Suppressor of

Statement

Reference

S^UAS.cGb, Scer\GAL4^Bx-MS1096 is a non-suppressor of wing phenotype of Egfr^DN.UAS.cBa, Scer\GAL4^Bx-MS1096, rho^UAS.cBa

(Guichard et al., 1999)

S^UAS.cGb, Scer\GAL4^Bx-MS1096 is a non-suppressor of wing vein phenotype of Egfr^DN.UAS.cBa, Scer\GAL4^Bx-MS1096, rho^UAS.cBa

(Guichard et al., 1999)

S^UAS.cGb, Scer\GAL4^Bx-MS1096 is a non-suppressor of wing vein L3 phenotype of Egfr^DN.UAS.cBa, Scer\GAL4^Bx-MS1096, rho^UAS.cBa

(Guichard et al., 1999)

S^UAS.cGb, Scer\GAL4^Bx-MS1096 is a non-suppressor of wing vein L4 phenotype of Egfr^DN.UAS.cBa, Scer\GAL4^Bx-MS1096, rho^UAS.cBa

(Guichard et al., 1999)

S^UAS.cGb, Scer\GAL4^Bx-MS1096 is a non-suppressor of wing vein L5 phenotype of Egfr^DN.UAS.cBa, Scer\GAL4^Bx-MS1096, rho^UAS.cBa

(Guichard et al., 1999)

Scer\GAL4^Bx-MS1096/S^UAS.cGb is a non-suppressor of phenotype of rho⁶

(Guichard et al., 1999)

Other

Statement

Reference

S^UAS.cGb, Scer\GAL4^kni.L2, rho^UAS.cBa has wing phenotype

(Lunde et al., 2003)

S^UAS.cGb, Scer\GAL4^kni.L2, rho^UAS.cBa has wing vein | ectopic phenotype

(Lunde et al., 2003)

S^UAS.cGb, Scer\GAL4^Bx-MS1096, grk^UAS.cQa has wing vein | ectopic phenotype

(Guichard et al., 2000)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of S^Scer\UAS.cGb and rho^Scer\UAS.cBa under the control of Scer\GAL4^kni.L2 results in ectopic veins that are displaced from the endogenous L2 wing vein by a few cells.

(Lunde et al., 2003)

Coexpression of S^Scer\UAS.cGb and stet^Scer\UAS.cGa when driven by Scer\GAL4^Bx-MS1096 during wing development gives a highly penetrant pupal lethality. Despite the lethality, fully differentiated wings can be dissected from pupal cases, revealing a strong ectopic vein phenotype. much stronger than seen in stet^Scer\UAS.cGa,Scer\GAL4^Bx-MS1096 wings alone. The addition of grk^Scer\UAS.cQa to S^Scer\UAS.cGb, Scer\GAL4^Bx-MS1096 flies causes an ectopic wing vein phenotype seen in these flies.

(Guichard et al., 2000)

Expression of S^Scer\UAS.cGb directed by Scer\GAL4^Bx-MS1096 in homozygous rho⁶ clones does not suppress the rho⁶ phenotype. Co-expression of Egfr^{DN.Scer\UAS.cBa}, S^Scer\UAS.cGb and rho^Scer\UAS.cBa driven by Scer\GAL4^Bx-MS1096, does not suppress the Egfr^{DN.Scer\UAS.cBa} induces loss of vein phenotype nor restore the rho^Scer\UAS.cBa induced ectopic vein phenotype. Expression of spi^hs.PG has no observable effect on S^Scer\UAS.cGb driven by Scer\GAL4^Bx-MS1096.

(Guichard et al., 1999)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

Scer\GAL4^Bx-MS1096/S^UAS.cGb rescues S^DN.UAS

(Guichard et al., 2002)

Comments

The vein truncation phenotype seen in S^DN.Scer\UAS; Scer\GAL4^Bx-MS1096 flies is rescued by S^Scer\UAS.cGb.

(Guichard et al., 2002)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

S^Scer\UAS.cGb

S^UAS.cGb

Name Synonyms

Saccharomyces cerevisiae UAS construct b of Guichard

(Guichard et al., 1999)

Secondary FlyBase IDs

References (4)

Report Sections

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