FlyBase Allele Report: Dmel\stet[UAS.cGa]

General Information

Symbol

Dmel\stet^UAS.cGa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Guichard

FlyBase ID

FBal0120374

Feature type

allele

Associated gene

Dmel\stet

Associated Insertion(s)

Carried in Construct

P{UAS-rho-2}

Key Links

Transgenic product class

genomic_DNA

(Guichard et al., 2000)

wild_type

(Guichard et al., 2000)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

genomic_DNA

(Guichard et al., 2000)

wild_type

(Guichard et al., 2000)

Mutagen

in vitro construct

(Guichard et al., 2000)

Progenitor genotype

Carried in construct

P{UAS-rho-2}

Cytology

Description

UASt sequences drive expression of the full genomic sequence of stet.

(Guichard et al., 2000)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

stet

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

dorsal appendage, with Scer\GAL4^CY2

(Guichard et al., 2000)

egg chorion, with Scer\GAL4^CY2

(Ghiglione et al., 2002, Guichard et al., 2000)

wing vein | ectopic, with Scer\GAL4^Bx-MS1096

(Guichard et al., 2000)

Detailed Description

Statement

Reference

Expression of stet^Scer\UAS.cGa in the follicle cells under the control of Scer\GAL4^CY2 results in weak dorsalisation of the eggshell.

(Ghiglione et al., 2002)

stet^Scer\UAS.cGa when driven by Scer\GAL4^Bx-MS1096 causes phenotypes in the wing. An ectopic vein phenotype is seen. When stet^Scer\UAS.cGa is driven by Scer\GAL4^CY2 dorsalisation of the eggshell is seen, resulting in thickened dorsal appendages which are more spread apart in mutant eggs than in wild-type. In more extreme cases white appendage like material fills in between the two appendages.

(Guichard et al., 2000)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Other

Statement

Reference

S^UAS.cBa, Scer\GAL4^CY2, grk^UAS.cGa, stet^UAS.cGa has lethal phenotype

(Ghiglione et al., 2002)

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^CY2, stet^UAS.cGa has egg chorion phenotype, enhanceable by Scer\GAL4^CY2/grk^UAS.cGa

(Ghiglione et al., 2002)

Scer\GAL4^Bx-MS1096, stet^UAS.cGa has wing vein | ectopic phenotype, enhanceable by grk^UAS.cQa/Scer\GAL4^Bx-MS1096

(Guichard et al., 2000)

Scer\GAL4^Bx-MS1096, stet^UAS.cGa has wing vein | ectopic phenotype, enhanceable by S^UAS.cGb, Scer\GAL4^Bx-MS1096

(Guichard et al., 2000)

Suppressed by

Statement

Reference

Scer\GAL4^Bx-MS1096, stet^UAS.cGa has wing vein | ectopic phenotype, suppressible by S^X155, Scer\GAL4^Bx-MS1096

(Guichard et al., 2000)

Enhancer of

Statement

Reference

Scer\GAL4^CY2/stet^UAS.cGa is an enhancer of egg chorion phenotype of Scer\GAL4^CY2, grk^UAS.cGa

(Ghiglione et al., 2002)

NOT Enhancer of

Statement

Reference

stet^UAS.cGa, Scer\GAL4^Bx-MS1096 is a non-enhancer of wing phenotype of Egfr^DN.UAS.cBa, Scer\GAL4^Bx-MS1096

(Guichard et al., 2000)

NOT Suppressor of

Statement

Reference

stet^UAS.cGa, Scer\GAL4^Bx-MS1096 is a non-suppressor of wing phenotype of Egfr^DN.UAS.cBa, Scer\GAL4^Bx-MS1096

(Guichard et al., 2000)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of stet^Scer\UAS.cGa and grk^Scer\UAS.cGa in the follicle cells under the control of Scer\GAL4^CY2 results in strong dorsalisation of the eggshell.

(Ghiglione et al., 2002)

Coexpression of S^Scer\UAS.cGb and stet^Scer\UAS.cGa when driven by Scer\GAL4^Bx-MS1096 during wing development gives a highly penetrant pupal lethality. Despite the lethality, fully differentiated wings can be dissected from pupal cases, revealing a strong ectopic vein phenotype. much stronger than seen in stet^Scer\UAS.cGa,Scer\GAL4^Bx-MS1096 wings alone. The addition of S^X155 to stet^Scer\UAS.cGa, Scer\GAL4^Bx-MS1096 flies almost completely suppresses the ectopic vein phenotype seen in these flies. The addition of grk^Scer\UAS.cQa to stet^Scer\UAS.cGa, Scer\GAL4^Bx-MS1096 flies causes an enhancement of the ectopic wing vein phenotype seen in these flies.

(Guichard et al., 2000)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

rho-2^Scer\UAS.cGa

(Ghiglione et al., 2002)

stet^Scer\UAS.cGa

stet^UAS.cGa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Guichard

(Guichard et al., 2000)

Secondary FlyBase IDs

References (2)

Report Sections

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