FB2024_03 , released June 25, 2024
Allele: Dmel\taraEP3463
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General Information
Symbol
Dmel\taraEP3463
Species
D. melanogaster
Name
FlyBase ID
FBal0103520
Feature type
allele
Associated gene
Dmel\tara
Associated Insertion(s)
P{EP}taraEP3463
Carried in Construct
Also Known As
EP(3)3463, EP3463
Key Links
Genomic Maps

Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
P-element activity
(Calgaro et al., 2002, Pena-Rangel et al., 2002, Gene Disruption Project members, 2001-, Abdelilah-Seyfried et al., 2000, Bourbon, 2000.1.21, Fernandez-Funez et al., 2000)
Progenitor genotype
Associated Insertion(s)
P{EP}taraEP3463
Cytology
Description

P{EP} insertion at position 18450 within the tara genomic sequence AF227213, insertion is near exon 1B.

(Calgaro et al., 2002)

The P{EP} insertion is in an intron about 16.3kb downstream of the first ATG, but only -553 with respect to second ATG. It is in the same orientation to the transcription unit.

(Fernandez-Funez et al., 2000)
Allele components
Component
Use(s)
Inserted element
P{EP}
Regulatory region(s)
UAS
Mutations Mapped to the Genome
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 1 )
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expression of taraEP3463 under the control of Scer\GAL4GMR.PF does not result in a rough eye phenotype.

      (Shulman and Feany, 2003)

      100% of homozygotes have held-out wings, 15% show truncation of wing vein L5 and 5% have ectopic ventral abdominal bristles.

      (Calgaro et al., 2002)

      Expression of taraEP3463 under the control of Scer\GAL4sca-537.4 results in the loss of external support cells of the scutellar macrochaetae without loss of the sheath cell. Sensory organs with no socket and one shaft, dot-like shaft morphology and large flat socket cells are seen.

      (Abdelilah-Seyfried et al., 2000)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      NOT Enhanced by
      Statement
      Reference

      Scer\GAL4sca-537.4, taraEP3463 has visible phenotype, non-enhanceable by H[+]/HE21

      (Abdelilah-Seyfried et al., 2000)

      Scer\GAL4sca-537.4, taraEP3463 has visible phenotype, non-enhanceable by N[+]/N55e11

      (Abdelilah-Seyfried et al., 2000)
      NOT suppressed by
      Statement
      Reference

      Scer\GAL4sca-537.4, taraEP3463 has visible phenotype, non-suppressible by H[+]/HE21

      (Abdelilah-Seyfried et al., 2000)

      Scer\GAL4sca-537.4, taraEP3463 has visible phenotype, non-suppressible by N[+]/N55e11

      (Abdelilah-Seyfried et al., 2000)
      Enhancer of
      Statement
      Reference

      taraEP3463/Scer\GAL4pnr-MD237 is an enhancer of visible phenotype of pnrMD237

      (Pena-Rangel et al., 2002)
      NOT Enhancer of
      Statement
      Reference

      taraEP3463, Scer\GAL4GMR.PF is a non-enhancer of visible phenotype of Hsap\MAPTV337M.UAS, Scer\GAL4GMR.PF

      (Shulman and Feany, 2003)
      Suppressor of
      Statement
      Reference

      taraEP3463/Scer\GAL4ap-md544 is a suppressor of visible phenotype of Bx1

      (Bejarano et al., 2008)
      NOT Suppressor of
      Statement
      Reference

      taraEP3463, Scer\GAL4GMR.PF is a non-suppressor of visible phenotype of Hsap\MAPTV337M.UAS, Scer\GAL4GMR.PF

      (Shulman and Feany, 2003)
      Phenotype Manifest In
      Enhancer of
      NOT Enhancer of
      Suppressor of
      Statement
      Reference

      taraEP3463/Scer\GAL4ap-md544 is a suppressor of wing margin phenotype of Bx1

      (Bejarano et al., 2008)
      NOT Suppressor of
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      pnrMD237/+ adults expressing taraEP3463 under the control of Scer\GAL4pnr-MD237 have a strong thoracic cleft.

      (Pena-Rangel et al., 2002)
      Xenogenetic Interactions
      Statement
      Reference

      Expression of taraEP3463 enhances the retinal phenotype seen when Hsap\HTT128Q.1-336.Scer\UAS is expressed under the control of Scer\GAL4GMR.PU.

      Expression of taraEP3463 enhances the retinal phenotype seen when Hsap\ATXN182Q.Scer\UAS is expressed under the control of Scer\GAL4GMR.PU.

      (Branco et al., 2008)

      The rough eye phenotype caused by expression of Hsap\MAPTV337M.Scer\UAS under the control of Scer\GAL4GMR.PF is not modified if the flies are also carrying taraEP3463.

      (Shulman and Feany, 2003)
      Complementation and Rescue Data
      Complements

      taraEP3427

      (Calgaro et al., 2002)
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer

      Selected as: a P{EP} insertion line that modifies the pnrMD237/+ phenotype when expressed using Scer\GAL4pnr-MD237.

      (Pena-Rangel et al., 2002)
      External Crossreferences and Linkouts ( 1 )
      Crossreferences
      GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      EP(3)3463
      (Shulman and Feany, 2003, Abdelilah-Seyfried et al., 2000, Bourbon, 2000.1.21)
      taraEP3463
      (Afonso et al., 2015)
      Name Synonyms
      Secondary FlyBase IDs
        References (11)