FlyBase Allele Report: Dmel\tum[UAS.cSa]

General Information

Symbol

Dmel\tum^UAS.cSa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Sotillos

FlyBase ID

FBal0122004

Feature type

allele

Associated gene

Dmel\tum

Associated Insertion(s)

Carried in Construct

P{UAS-RacGAP.S}

Key Links

Transgenic product class

cDNA

(Sotillos and Campuzano, 2000)

wild_type

(Sotillos and Campuzano, 2000)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Sotillos and Campuzano, 2000)

wild_type

(Sotillos and Campuzano, 2000)

Mutagen

in vitro construct

(Sotillos and Campuzano, 2000)

Progenitor genotype

Carried in construct

P{UAS-RacGAP.S}

Cytology

Description

UASt governs the expression of tum cDNA.

(Sotillos and Campuzano, 2000)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

tum

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

campaniform sensillum, with Scer\GAL4^en-e16E

(Sotillos and Campuzano, 2000)

wing, with Scer\GAL4^ap-md544

(Jones et al., 2010)

wing, with Scer\GAL4^Bx-MS1096

(Sotillos and Campuzano, 2000)

wing, with Scer\GAL4^C-765

(Sotillos and Campuzano, 2000)

wing, with Scer\GAL4^en-e16E

(Sotillos and Campuzano, 2000)

wing vein, with Scer\GAL4^Bx-MS1096

(Sotillos and Campuzano, 2000)

wing vein, with Scer\GAL4^C-765

(Sotillos and Campuzano, 2000)

wing vein, with Scer\GAL4^en-e16E

(Sotillos and Campuzano, 2000)

Detailed Description

Statement

Reference

Overexpression of tum^Scer\UAS.cSa driven by either Scer\GAL4^e22c or Scer\GAL4^ap-md544 has little effect on viability or on embryonic epidermal patterning.

Overexpression of tum^Scer\UAS.cSa driven by Scer\GAL4^ap-md544 produces a decrease in the overall size of the wing.

Overexpression of tum^{ΔPav.Scer\UAS} driven by Scer\GAL4^ap-md544 does not reduce wing size.

(Jones et al., 2010)

When expression is driven by Scer\GAL4^Bx-MS1096 or Scer\GAL4^C-765 the corrugation of the adult vein is eliminated, and the intervein distance decreased. When expression is driven by Scer\GAL4^en-e16E the adult veins are thinned, the wings are reduced in size and the sensory organs reduced in number.

(Sotillos and Campuzano, 2000)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

tum^UAS.cSa, Scer\GAL4^sd-SG29.1 is an enhancer of visible phenotype of Rac1^N17.UAS, Scer\GAL4^sd-SG29.1

(Sotillos and Campuzano, 2000)

Suppressor of

Statement

Reference

tum^UAS.cSa, Scer\GAL4^en-e16E is a suppressor | partially of increased cell death phenotype of Hsap\CDKN1A^UAS.cHa, Scer\GAL4^en-e16E

(Sotillos and Campuzano, 2000)

tum^UAS.cSa, Scer\GAL4^en-e16E is a suppressor | partially of abnormal planar polarity phenotype of Hsap\CDKN1A^UAS.cHa, Scer\GAL4^en-e16E

(Sotillos and Campuzano, 2000)

tum^UAS.cSa, Scer\GAL4^en-e16E is a suppressor | partially of visible phenotype of Hsap\CDKN1A^UAS.cHa, Scer\GAL4^en-e16E

(Sotillos and Campuzano, 2000)

tum^UAS.cSa, Scer\GAL4^sd-SG29.1 is a suppressor of lethal phenotype of Rac1^UAS.cLa, Scer\GAL4^sd-SG29.1

(Sotillos and Campuzano, 2000)

Other

Statement

Reference

Scer\GAL4^ap-md544, pav^UASp.mGFP6, tum^UAS.cSa has lethal | P-stage phenotype

(Jones et al., 2010)

Scer\GAL4^e22c, pav^UASp.mGFP6, tum^UAS.cSa has lethal | embryonic stage phenotype

(Jones et al., 2010)

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^e22c, pav^UASp.mGFP6, tum^UAS.cSa has abdominal ventral denticle belt | embryonic stage phenotype, enhanceable by wg[+]/wg^l-17

(Jones et al., 2010)

Scer\GAL4^ap-md544, tum^UAS.cSa has wing phenotype, enhanceable by Scer\GAL4^ap-md544/pav^UASp.mGFP6

(Jones et al., 2010)

Enhancer of

Statement

Reference

tum^UAS.cSa, Scer\GAL4^sd-SG29.1 is an enhancer of wing phenotype of Rac1^N17.UAS, Scer\GAL4^sd-SG29.1

(Sotillos and Campuzano, 2000)

Suppressor of

Statement

Reference

Scer\GAL4^arm.PS/tum^UAS.cSa is a suppressor | partially of abdominal ventral denticle belt phenotype of Scer\GAL4^arm.PS, arm^{S10.UAS.Tag:MYC}

(Jones et al., 2010)

tum^UAS.cSa, Scer\GAL4^en-e16E is a suppressor | partially of wing & cell phenotype of Hsap\CDKN1A^UAS.cHa, Scer\GAL4^en-e16E

(Sotillos and Campuzano, 2000)

tum^UAS.cSa, Scer\GAL4^en-e16E is a suppressor | partially of wing & trichome phenotype of Hsap\CDKN1A^UAS.cHa, Scer\GAL4^en-e16E

(Sotillos and Campuzano, 2000)

tum^UAS.cSa, Scer\GAL4^en-e16E is a suppressor | partially of wing phenotype of Hsap\CDKN1A^UAS.cHa, Scer\GAL4^en-e16E

(Sotillos and Campuzano, 2000)

Other

Statement

Reference

Scer\GAL4^e22c, pav^UASp.mGFP6, tum^UAS.cSa has abdominal ventral denticle belt | embryonic stage phenotype

(Jones et al., 2010)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of tum^Scer\UAS.cSa with pav^{Scer\UAS.P\T.T:Avic\GFP-m6} in the developing embryonic epidermis driven by Scer\GAL4^e22c results in embryonic lethality.

Co-expression of tum^Scer\UAS.cSa with pav^{Scer\UAS.P\T.T:Avic\GFP-m6} in the developing embryonic epidermis driven by Scer\GAL4^e22c causes reduction of the segmental naked cuticle expanse. The penetrance of this segmentation defect is increased when embryos are heterozygous for wg^l-17.

Co-expression of tum^Scer\UAS.cSa with pav^{Scer\UAS.P\T.T:Avic\GFP-m6} driven by Scer\GAL4^ap-md544 results in a profound decrease in eclosion rate. The few flies that eclose show wings that are reduced in size and blistered.

Co-expression of tum^Scer\UAS.cSa driven by Scer\GAL4^arm.PS partially blocks the cell-fate transformation induced by arm^{S10.Scer\UAS.T:Hsap\MYC} expression and allows some denticle production.

(Jones et al., 2010)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

Scer\GAL4^e22c/tum^UAS.cSa rescues tum^AR2

(Jones et al., 2010)

Scer\GAL4^en-e16E/tum^UAS.cSa rescues tum^ΔE1E.UAS

(Sotillos and Campuzano, 2000)

Partially rescues

Scer\GAL4^e22c/tum^UAS.cSa partially rescues tum^AR2

(Jones and Bejsovec, 2005)

Scer\GAL4^en-e16E/tum^UAS.cSa partially rescues tum^ΔE1E.UAS/Df(2R)CX1

(Sotillos and Campuzano, 2000)

Comments

Expression of tum^Scer\UAS.cSa driven by Scer\GAL4^e22c rescues the epidermal pattern defects of tum^AR2 mutants.

(Jones et al., 2010)

Expression of RacGAP50C^Scer\UAS.cSa under the control of Scer\GAL4^e22c partially rescues the lethality and completely rescues the cuticle patterning defects of RacGAP50C^AR2 embryos.

(Jones and Bejsovec, 2005)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

RacGAP50C^Scer\UAS.cSa

RacGAP^Scer\UAS.cSa

(Sotillos and Campuzano, 2000)

tum^Scer\UAS.cSa

tum^UAS.cSa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Sotillos

(Sotillos and Campuzano, 2000)

Secondary FlyBase IDs

References (3)

Report Sections

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