FlyBase Allele Report: Dmel\tum[ΔE1E.UAS]

General Information

Symbol

Dmel\tum^ΔE1E.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0122006

Feature type

allele

Associated gene

Dmel\tum

Associated Insertion(s)

Carried in Construct

P{UAS-RacGAP.ΔE1E}

Key Links

Transgenic product class

dominant_negative_variant

(Sotillos and Campuzano, 2000)

inframe_deletion

(Sotillos and Campuzano, 2000)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

dominant_negative_variant

(Sotillos and Campuzano, 2000)

inframe_deletion

(Sotillos and Campuzano, 2000)

Mutagen

in vitro construct

(Sotillos and Campuzano, 2000)

Progenitor genotype

Carried in construct

P{UAS-RacGAP.ΔE1E}

Cytology

Description

UASt governs the expression of a dominant negative form of tum cDNA with a deletion of amino acids 405-407.

(Sotillos and Campuzano, 2000)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

tum

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

sensillum campaniformium & wing vein L3 | supernumerary, with Scer\GAL4^69B

(Sotillos and Campuzano, 2000)

sensillum campaniformium & wing vein L3 | supernumerary, with Scer\GAL4^Bx-MS1096

(Sotillos and Campuzano, 2000)

sensillum campaniformium & wing vein L3 | supernumerary, with Scer\GAL4^en-e16E

(Sotillos and Campuzano, 2000)

sensillum campaniformium & wing vein L4 | supernumerary, with Scer\GAL4^69B

(Sotillos and Campuzano, 2000)

sensillum campaniformium & wing vein L4 | supernumerary, with Scer\GAL4^Bx-MS1096

(Sotillos and Campuzano, 2000)

sensillum campaniformium & wing vein L4 | supernumerary, with Scer\GAL4^en-e16E

(Sotillos and Campuzano, 2000)

sensillum campaniformium & wing vein L5 | supernumerary, with Scer\GAL4^69B

(Sotillos and Campuzano, 2000)

sensillum campaniformium & wing vein L5 | supernumerary, with Scer\GAL4^Bx-MS1096

(Sotillos and Campuzano, 2000)

sensillum campaniformium & wing vein L5 | supernumerary, with Scer\GAL4^en-e16E

(Sotillos and Campuzano, 2000)

wing, with Scer\GAL4^69B

(Sotillos and Campuzano, 2000)

wing, with Scer\GAL4^Bx-MS1096

(Sotillos and Campuzano, 2000)

wing, with Scer\GAL4^en-e16E

(Sotillos and Campuzano, 2000)

wing & cell, with Scer\GAL4^69B

(Sotillos and Campuzano, 2000)

wing & cell, with Scer\GAL4^Bx-MS1096

(Sotillos and Campuzano, 2000)

wing & cell, with Scer\GAL4^en-e16E

(Sotillos and Campuzano, 2000)

wing & trichome, with Scer\GAL4^69B

(Sotillos and Campuzano, 2000)

wing & trichome, with Scer\GAL4^Bx-MS1096

(Sotillos and Campuzano, 2000)

wing & trichome, with Scer\GAL4^en-e16E

(Sotillos and Campuzano, 2000)

wing disc, with Scer\GAL4^en-e16E

(Sotillos and Campuzano, 2000)

wing vein, with Scer\GAL4^69B

(Sotillos and Campuzano, 2000)

wing vein, with Scer\GAL4^Bx-MS1096

(Sotillos and Campuzano, 2000)

wing vein, with Scer\GAL4^en-e16E

(Sotillos and Campuzano, 2000)

Detailed Description

Statement

Reference

When expression is driven by Scer\GAL4^en-e16E wings are notched, wing veins are increased in width and can be fused and extra wing vein material appears connecting neighboring veins. Extra campaniform sensillae develop in association with wing veins L3, L4 and L5. Cells are enlarged (visible as early as the imaginal disc), and trichomes show altered polarity and number. When expression is driven by Scer\GAL4^Bx-MS1096 or Scer\GAL4^69B the result is the same. When expression is driven by Scer\GAL4^en-e16E wing discs show increased levels of apoptosis, and decreased viability as indicated by clonal analysis.

(Sotillos and Campuzano, 2000)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Scer\GAL4^en-e16E, tum^ΔE1E.UAS has visible phenotype, enhanceable by Df(3R)dsx10M

(Sotillos and Campuzano, 2000)

Scer\GAL4^en-e16E, tum^ΔE1E.UAS has visible phenotype, enhanceable by argos[+]/aos^p1

(Sotillos and Campuzano, 2000)

Suppressed by

Statement

Reference

Scer\GAL4^en-e16E, tum^ΔE1E.UAS has visible phenotype, suppressible by Cdc42³

(Sotillos and Campuzano, 2000)

Scer\GAL4^en-e16E, tum^ΔE1E.UAS has increased cell death phenotype, suppressible by CycE^UAS.cLa, Scer\GAL4^en-e16E

(Sotillos and Campuzano, 2000)

Scer\GAL4^en-e16E, tum^ΔE1E.UAS has abnormal mitotic cell cycle phenotype, suppressible by CycE^UAS.cLa, Scer\GAL4^en-e16E

(Sotillos and Campuzano, 2000)

Scer\GAL4^en-e16E, tum^ΔE1E.UAS has increased cell death phenotype, suppressible by BacA\p35^UAS.cHa, Scer\GAL4^en-e16E

(Sotillos and Campuzano, 2000)

Scer\GAL4^en-e16E, tum^ΔE1E.UAS has visible phenotype, suppressible | partially by Pak⁴

(Sotillos and Campuzano, 2000)

Scer\GAL4^en-e16E, tum^ΔE1E.UAS has visible phenotype, suppressible | partially by vn[+]/vn¹/rho^7M43/rho[+]

(Sotillos and Campuzano, 2000)

Enhancer of

Statement

Reference

Scer\GAL4^en-e16E/tum^ΔE1E.UAS is an enhancer of visible phenotype of Rac1^UAS.cLa, Scer\GAL4^sd-SG29.1

(Sotillos and Campuzano, 2000)

Suppressor of

Statement

Reference

tum^ΔE1E.UAS, Scer\GAL4^en-e16E is a suppressor | partially of visible phenotype of Raf^K497M.UAS, Scer\GAL4^en-e16E

(Sotillos and Campuzano, 2000)

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^en-e16E, tum^ΔE1E.UAS has wing vein phenotype, enhanceable by Df(3R)dsx10M

(Sotillos and Campuzano, 2000)

Scer\GAL4^en-e16E, tum^ΔE1E.UAS has wing phenotype, enhanceable by Df(3R)dsx10M

(Sotillos and Campuzano, 2000)

Scer\GAL4^en-e16E, tum^ΔE1E.UAS has sensillum campaniformium & wing vein | supernumerary phenotype, enhanceable by argos[+]/aos^p1

(Sotillos and Campuzano, 2000)

Suppressed by

Statement

Reference

Scer\GAL4^en-e16E, tum^ΔE1E.UAS has wing & cell phenotype, suppressible by CycE^UAS.cLa, Scer\GAL4^en-e16E

(Sotillos and Campuzano, 2000)

Scer\GAL4^69B, tum^ΔE1E.UAS has wing & trichome phenotype, suppressible by CycE^UAS.cLa, Scer\GAL4^69B

(Sotillos and Campuzano, 2000)

Scer\GAL4^en-e16E, tum^ΔE1E.UAS has wing vein phenotype, suppressible by Cdc42³

(Sotillos and Campuzano, 2000)

Scer\GAL4^en-e16E, tum^ΔE1E.UAS has wing vein phenotype, suppressible | partially by Pak⁴

(Sotillos and Campuzano, 2000)

Scer\GAL4^en-e16E, tum^ΔE1E.UAS has wing phenotype, suppressible by Cdc42³

(Sotillos and Campuzano, 2000)

Scer\GAL4^en-e16E, tum^ΔE1E.UAS has wing phenotype, suppressible | partially by Pak⁴

(Sotillos and Campuzano, 2000)

Scer\GAL4^en-e16E, tum^ΔE1E.UAS has wing phenotype, suppressible | partially by vn[+]/vn¹/rho^7M43/rho[+]

(Sotillos and Campuzano, 2000)

NOT suppressed by

Statement

Reference

Scer\GAL4^en-e16E, tum^ΔE1E.UAS has wing vein phenotype, non-suppressible by CycE^UAS.cLa, Scer\GAL4^en-e16E

(Sotillos and Campuzano, 2000)

Enhancer of

Statement

Reference

Scer\GAL4^en-e16E/tum^ΔE1E.UAS is an enhancer of campaniform sensillum phenotype of Rac1^UAS.cLa, Scer\GAL4^sd-SG29.1

(Sotillos and Campuzano, 2000)

Scer\GAL4^en-e16E/tum^ΔE1E.UAS is an enhancer of wing vein phenotype of Rac1^UAS.cLa, Scer\GAL4^sd-SG29.1

(Sotillos and Campuzano, 2000)

Scer\GAL4^en-e16E/tum^ΔE1E.UAS is an enhancer of wing phenotype of Rac1^UAS.cLa, Scer\GAL4^sd-SG29.1

(Sotillos and Campuzano, 2000)

Suppressor of

Statement

Reference

tum^ΔE1E.UAS, Scer\GAL4^en-e16E is a suppressor | partially of wing vein L4 phenotype of Raf^K497M.UAS, Scer\GAL4^en-e16E

(Sotillos and Campuzano, 2000)

tum^ΔE1E.UAS, Scer\GAL4^en-e16E is a suppressor | partially of wing vein L5 phenotype of Raf^K497M.UAS, Scer\GAL4^en-e16E

(Sotillos and Campuzano, 2000)

tum^ΔE1E.UAS, Scer\GAL4^en-e16E is a suppressor | partially of wing phenotype of Raf^K497M.UAS, Scer\GAL4^en-e16E

(Sotillos and Campuzano, 2000)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

tum^ΔE1E.UAS is rescued by Scer\GAL4^en-e16E/tum^UAS.cSa

(Sotillos and Campuzano, 2000)

Partially rescued by

tum^ΔE1E.UAS/Df(2R)CX1 is partially rescued by Scer\GAL4^en-e16E/tum^UAS.cSa

(Sotillos and Campuzano, 2000)

Partially rescues

tum^ΔE1E.UAS/Scer\GAL4^arm.PS partially rescues tum^AR2

(Jones and Bejsovec, 2005)

tum^ΔE1E.UAS/Scer\GAL4^e22c partially rescues tum^AR2

(Jones and Bejsovec, 2005)

Comments

Expression of RacGAP50C^{ΔE1E.Scer\UAS} under the control of either Scer\GAL4^e22c or Scer\GAL4^arm.PS partially rescues the cuticle patterning defects of RacGAP50C^AR2 embryos, although there is no rescue of embryonic lethality.

(Jones and Bejsovec, 2005)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Comments

Effect stronger than for RacGAP50C^{P417Q.Scer\UAS}.

(Sotillos and Campuzano, 2000)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

RacGAP50C^{ΔE1E.Scer\UAS}

RacGAP^{ΔE1E.Scer\UAS}

(Sotillos and Campuzano, 2000)

tum^{ΔE1E.Scer\UAS}

tum^ΔE1E.UAS

Name Synonyms

Secondary FlyBase IDs

References (3)

Report Sections

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