FlyBase Allele Report: Dmel\sgg[EP1576]

General Information

Symbol

Dmel\sgg^EP1576

Species

D. melanogaster

Name

FlyBase ID

FBal0123706

Feature type

allele

Associated gene

Dmel\sgg

Associated Insertion(s)

P{EP}sgg^EP1576

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(Parker et al., 2002, Gene Disruption Project members, 2001-, Martinek et al., 2001)

Progenitor genotype

Associated Insertion(s)

P{EP}sgg^EP1576

Cytology

Description

The P{EP} insertion site is 693bp downstream of the sgg10 translation start site, 1022bp upstream of the sggy isoform translation start site.

(Martinek et al., 2001)

Allele components

Component

Use(s)

Inserted element

P{EP}

misexpression element

Regulatory region(s)

UAS

binary expression system - regulatory region

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

X:2,642,700..2,642,700 [+]

(Wu et al., 2022, Petsakou et al., 2015, Stoleru et al., 2007, Wolf et al., 2007, Singh et al., 2005, Stoleru et al., 2005, Wülbeck et al., 2005, Parker et al., 2002, Gene Disruption Project members, 2001-, Martinek et al., 2001)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

Flies expressing sgg^EP1576 under the control of Scer\GAL4^P0.5.Pdf (limited to the adult stages using Scer\GAL80^ts.αTub84B) have a short period behavioural rhythm of 21.4 hours in complete darkness (DD).

(Petsakou et al., 2015)

Approximately 50% of flies expressing sgg^EP1576 under the control of Scer\GAL4^tim.PE show rhythmic locomotor activity under constant light conditions, in contrast to wild-type flies, which are uniformly arrhythmic under these conditions.

Flies expressing sgg^EP1576 under the control of Scer\GAL4^tim.PE in a Scer\GAL80^Pdf.PS background show rhythmic locomotor activity under constant light conditions.

Flies expressing sgg^EP1576 under the control of Scer\GAL4^tim.PE in a Scer\GAL80^cry.PS background are completely arrhythmic for locomotor activity under constant light conditions, as are wild-type flies.

Flies expressing sgg^EP1576 under the control of Scer\GAL4^tim.PE have a locomotor activity period of 20.3 hours under constant darkness conditions.

Flies expressing sgg^EP1576 under the control of Scer\GAL4^P2.4.Pdf are completely arrhythmic for locomotor activity under constant light conditions, as are wild-type flies.

The phase response curve of flies expressing sgg^EP1576 under the control of Scer\GAL4^P2.4.Pdf is indistinguishable from that of controls.

The phase response curve of flies expressing sgg^EP1576 under the control of Scer\GAL4^tim.PE in either a wild-type or Scer\GAL80^Pdf.PS background is altered compared to wild-type, having attenuated delay zone amplitudes and earlier transitions (breakpoints) between the delay and advance zones.

Flies expressing sgg^EP1576 under the control of Scer\GAL4^P2.4.Pdf show robust advancement of morning anticipation under standard light-dark conditions, but show no significant change in the phase of the evening output. If these flies are entrained to a short photoperiod regime (winter-like; light:dark 10:14 hours), the evening and morning activities are advanced. If these flies are entrained to a long photoperiod regime (summer-like; light:dark 14:10 hours), principally the morning activity is enhanced.

Flies expressing sgg^EP1576 under the control of Scer\GAL4^tim.PE in either a wild-type or Scer\GAL80^Pdf.PS background show robust advancement of morning anticipation under standard light-dark conditions and the evening activity is also advanced. If these flies are entrained to a short photoperiod regime (winter-like; light:dark 10:14 hours), only the evening activity is advanced. If these flies are entrained to a long photoperiod regime (summer-like; light:dark 14:10 hours), both the evening and morning activities are advanced.

(Stoleru et al., 2007)

sgg^EP1576 flies show wild-type olfactory habituation.

(Wolf et al., 2007)

Expression of sgg^EP1576 under the control of Scer\GAL4^ey.PH results in a normal looking eye.

(Singh et al., 2005)

sgg^EP1576, Scer\GAL4^tim.PE mutant flies in constant darkness (DD) exhibit a period 3h shorter than control flies. Both the average length of subjective day and the period are reduced. sgg^EP1576, Scer\GAL4^P2.4.Pdf mutant flies in constant darkness (DD) exhibit a period a couple of hours shorter than control flies. The period of evening activity is significantly shorter than controls in these flies. sgg^EP1576, Scer\GAL4^cry.PE mutant flies in constant darkness (DD) has no effect on circadian rhythms.

(Stoleru et al., 2005)

Only 57% of adults expressing sgg^EP1576 under the control of Scer\GAL4^tim.PE have show rhythmic locomotor activity under free-running conditions. Those animals that are rhythmic have a shorter locomotor activity period than normal.

(Wülbeck et al., 2005)

When sgg^EP1576 is driven by Scer\GAL4^tim.Scer\UAS or Scer\GAL4^tim.PE the locomotor activity period is decreased by approximately 3 hours.

(Martinek et al., 2001)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Scer\GAL4^P0.5.Pdf, sgg^EP1576 has abnormal circadian behavior phenotype, suppressible by Rho1^UAS.cMa, Scer\GAL4^P0.5.Pdf

(Petsakou et al., 2015)

Scer\GAL4^tim.PE, sgg^EP1576 has abnormal locomotor rhythm phenotype, suppressible | partially by cry^b

(Stoleru et al., 2007)

Suppressor of

Statement

Reference

sgg^EP1576/Scer\GAL4^GMR.PF is a suppressor of abnormal size phenotype of Scer\GAL4^GMR.PF, egr^UAS.cIa

(Zhang et al., 2015)

sgg^EP1576/Scer\GAL4^GMR.PF is a suppressor of visible phenotype of Scer\GAL4^GMR.PF, egr^UAS.cIa

(Zhang et al., 2015)

sgg^EP1576/Scer\GAL4^GMR.PF is a suppressor of abnormal size phenotype of Scer\GAL4^GMR.PF, hep^CA.UAS

(Zhang et al., 2015)

sgg^EP1576/Scer\GAL4^GMR.PF is a suppressor of visible phenotype of Scer\GAL4^GMR.PF, hep^CA.UAS

(Zhang et al., 2015)

Scer\GAL4^ptc-559.1/sgg^EP1576 is a suppressor of visible phenotype of Scer\GAL4^ptc-559.1, puc^RNAi.UAS.cUa

(Zhang et al., 2015)

sgg^EP1576/sgg[+] is a suppressor of visible phenotype of Hsap\MAPT^GMR.Ex.PJ

(Ambegaokar and Jackson, 2011)

sgg^EP1576/Scer\GAL4^ey.PH is a suppressor of visible | dominant phenotype of L²

(Singh et al., 2005)

Phenotype Manifest In

Suppressor of

Statement

Reference

sgg^EP1576/Scer\GAL4^GMR.PF is a suppressor of eye phenotype of Scer\GAL4^GMR.PF, egr^UAS.cIa

(Zhang et al., 2015)

sgg^EP1576/Scer\GAL4^GMR.PF is a suppressor of eye phenotype of Scer\GAL4^GMR.PF, hep^CA.UAS

(Zhang et al., 2015)

Scer\GAL4^ptc-559.1/sgg^EP1576 is a suppressor of anterior crossvein phenotype of Scer\GAL4^ptc-559.1, puc^RNAi.UAS.cUa

(Zhang et al., 2015)

sgg^EP1576/sgg[+] is a suppressor of eye phenotype of Hsap\MAPT^GMR.Ex.PJ

(Ambegaokar and Jackson, 2011)

sgg^EP1576/Scer\GAL4^ey.PH is a suppressor of eye | ventral phenotype of L²

(Singh et al., 2005)

sgg^EP1576/Scer\GAL4^GMR.PF is a suppressor of eye phenotype of Scer\GAL4^GMR.PF, wg^UAS.cKa

(Parker et al., 2002)

sgg^EP1576/Scer\GAL4^GMR.PF is a suppressor of interommatidial bristle phenotype of wg^sev.PC

(Parker et al., 2002)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of Rho1^Scer\UAS.cMa suppresses the shortening of the behavioural rhythm period seen when sgg^EP1576 is expressed under the control of Scer\GAL4^P0.5.Pdf (limited to the adult stages using Scer\GAL80^ts.αTub84B)in complete darkness (DD).

(Petsakou et al., 2015)

Flies expressing sgg^EP1576 under the control of Scer\GAL4^tim.PE in a cry^b background have a locomotor activity period of 22.6 hours under constant darkness conditions, which is longer (and closer to that of wild type) than the locomotor activity period of 20.3 hours seen under these conditions in flies expressing sgg^EP1576 under the control of Scer\GAL4^tim.PE in wild-type background.

(Stoleru et al., 2007)

sgg^EP1576, Scer\GAL4^tim.PE, Scer\GAL80^Pdf.PS mutant flies in constant darkness (DD) exhibit an evening phase similar to sgg^EP1576, Scer\GAL4^tim.PE animals but have a period of 23.8 hours. The length of subjective day for these animals is about 10.35h. sgg^EP1576, Scer\GAL4^tim.PE, Scer\GAL80^cry.PS mutant flies in constant darkness (DD) exhibit an evening phase similar to controls.

(Stoleru et al., 2005)

More than 80% of adults expressing sgg^EP1576 under the control of Scer\GAL4^tim.PE in a tim^blind background rapidly become arrhythmic for locomotor activity after they are placed in constant darkness conditions. The few animals that are rhythmic have a locomotor activity period that is intermediate between that of tim^blind homozygotes and that of adults expressing sgg^EP1576 under the control of Scer\GAL4^tim.PE in a wild-type background.

(Wülbeck et al., 2005)

Xenogenetic Interactions

Statement

Reference

One copy of sgg^EP1576 suppresses the rough eye phenotype seen in flies expressing Hsap\MAPT^GMR.Ex.PJ.

(Ambegaokar and Jackson, 2011)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

11008

P{EP}sgg^EP1576 w¹¹¹⁸

Notes on Origin

Discoverer

Comments

Excision of the P{EP}sgg^EP1576 insertion reverts the phenotype to wild-type.

(Martinek et al., 2001)

With respect to circadian period length, the following alleles can be ranked from longest to shortest: sgg^EP1576 = sgg^twkr/+ = sgg¹/sgg^EP1576 < sgg^twkr/sgg^twkr < sgg¹/sgg^twkr.

(Wolf et al., 2007)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

EP(X)1576

(Stanewsky, 2003, Parker et al., 2002)

Sgg^EP1576

(Zhang et al., 2015)

sgg^EP1576

(Hendricks and Liebl, 2024, Ambegaokar and Jackson, 2011)

sgg^EP

(Wu et al., 2022)

Name Synonyms

Secondary FlyBase IDs

References (15)

Report Sections

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