FlyBase Allele Report: Dmel\nmo[c5-1.UAS]

General Information

Symbol

Dmel\nmo^c5-1.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0123768

Feature type

allele

Associated gene

Dmel\nmo

Associated Insertion(s)

Carried in Construct

P{UAS-nmo.c5-1}

Also Known As

UAS-Nemo, UAS-nmo^C5-1e

Key Links

Transgenic product class

cDNA

(Verheyen et al., 2001)

wild_type

(Verheyen et al., 2001)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Verheyen et al., 2001)

wild_type

(Verheyen et al., 2001)

Mutagen

in vitro construct

(Verheyen et al., 2001)

Progenitor genotype

Carried in construct

P{UAS-nmo.c5-1}

Cytology

Description

UASt regulatory sequences drive expression of a full length cDNA corresponding to nmo isoform c5-1.

(Verheyen et al., 2001)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

nmo

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

abdominal tergite & macrochaeta, with Scer\GAL4^69B

(Verheyen et al., 2001)

abdominal tergite & microchaeta, with Scer\GAL4^69B

(Verheyen et al., 2001)

denticle, with Scer\GAL4^69B

(Mirkovic et al., 2002)

embryo | dorsal closure stage, with Scer\GAL4^69B

(Verheyen et al., 2001)

embryonic/first instar larval cuticle, with Scer\GAL4^69B

(Mirkovic et al., 2002, Verheyen et al., 2001)

embryonic head, with Scer\GAL4^69B

(Mirkovic et al., 2002)

posterior crossvein, with Scer\GAL4^32B

(Verheyen et al., 2001)

posterior crossvein, with Scer\GAL4^69B

(Mirkovic et al., 2002, Verheyen et al., 2001)

scutellar bristle, with Scer\GAL4^ap-md544

(Zeng and Verheyen, 2004)

scutum & macrochaeta, with Scer\GAL4^ap-md544

(Zeng and Verheyen, 2004)

ventral denticle belt, with Scer\GAL4^69B

(Verheyen et al., 2001)

wing, with Scer\GAL4^69B

(Mirkovic et al., 2002, Verheyen et al., 2001)

wing, with Scer\GAL4^ap-md544

(Zeng and Verheyen, 2004)

wing | anterior, with Scer\GAL4^69B

(Verheyen et al., 2001)

wing margin, with Scer\GAL4^sd-SG29.1

(Zeng and Verheyen, 2004)

wing vein | ectopic, with Scer\GAL4^69B

(Mirkovic et al., 2002, Verheyen et al., 2001)

Detailed Description

Statement

Reference

nmo^{c5-1.Scer\UAS}; Scer\GAL4^sd-SG29.1 flies have distally notched wings, but nmo^{c5-1.Scer\UAS}; Scer\GAL4^71B flies have normal wings. nmo^{c5-1.Scer\UAS}; Scer\GAL4^ap-md544 flies lack many notum macrochaetae. In nmo^{c5-1.Scer\UAS}; Scer\GAL4^ap-md544 flies, the wing blades do not appose properly resulting in a large blister.

(Zeng and Verheyen, 2004)

Ectopic expression of nmo in epidermal cells of the embryo using the Scer\GAL4^69B with nmo^{c5-1.Scer\UAS} results in severe head malformations and loss of denticle structures and denticle diversity and an increase in embryonic cell death. In Scer\GAL4^69B, nmo^{c5-1.Scer\UAS} adults, the wing blade is smaller and narrower than wild-type and shows extra vein material along L2 and a severe reduction in the size of the posterior cross vein.

(Mirkovic et al., 2002)

Expression of nmo^{c5-1.Scer\UAS} under the control of Scer\GAL4^32B or Scer\GAL4^69B results in loss of posterior crossvein tissue. Expression of nmo^{c5-1.Scer\UAS} under the control of Scer\GAL4^69B results in ectopic vein formation in the anterior part of the wing and the wing blade elongates and is more narrow and pointed than normal. Expression of nmo^{c5-1.Scer\UAS} under the control of Scer\GAL4^69B results in severe patterning defects in the abdomen, including disrupted polarity of microchaetae and macrochaetae in the tergites. Expression of two copies of nmo^{c5-1.Scer\UAS} under the control of Scer\GAL4^69B results in embryonic lethality with variable early phenotypes, including denticle belt defects, failure to produce cuticle and defects in dorsal closure.

(Verheyen et al., 2001)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Scer\GAL4^69B, nmo^c5-1.UAS has visible phenotype, suppressible by Df(2L)flp147E/+

(Mirkovic et al., 2002)

Scer\GAL4^69B, nmo^c5-1.UAS has visible phenotype, suppressible by Jra¹/Jra[+]

(Mirkovic et al., 2002)

Scer\GAL4^69B, nmo^c5-1.UAS has visible phenotype, suppressible by Tak1^K46R.UAS/Scer\GAL4^69B

(Mirkovic et al., 2002)

Scer\GAL4^69B, nmo^c5-1.UAS has abnormal cell death | embryonic stage phenotype, suppressible by BacA\p35^UAS.cHa, Scer\GAL4^69B

(Mirkovic et al., 2002)

Scer\GAL4^69B, nmo^c5-1.UAS has visible phenotype, suppressible by bsk¹/bsk[+]

(Mirkovic et al., 2002)

Scer\GAL4^69B, nmo^c5-1.UAS has visible phenotype, suppressible by msn[+]/msn^j1E2

(Mirkovic et al., 2002)

Suppressor of

Statement

Reference

Scer\GAL4^hs.2sev/nmo^c5-1.UAS is a suppressor | partially of abnormal planar polarity phenotype of Scer\GAL4^hs.2sev, sca^UAS.cEa

(Chou and Chien, 2002)

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^ap-md544, nmo^c5-1.UAS has scutellar bristle phenotype, enhanceable by Axn^A2.UAS, Scer\GAL4^ap-md544

(Zeng and Verheyen, 2004)

Scer\GAL4^69B, nmo^c5-1.UAS has wing vein | ectopic phenotype, enhanceable by Tak1^K46R.UAS/Scer\GAL4^69B

(Mirkovic et al., 2002)

Suppressed by

Statement

Reference

Scer\GAL4^ap-md544, nmo^c5-1.UAS has scutellar bristle phenotype, suppressible by sgg^M11/sgg[+]

(Zeng and Verheyen, 2004)

Scer\GAL4^ap-md544, nmo^c5-1.UAS has scutum & macrochaeta phenotype, suppressible by sgg^M11/sgg[+]

(Zeng and Verheyen, 2004)

Scer\GAL4^ap-md544, nmo^c5-1.UAS has wing phenotype, suppressible | partially by sgg^M11/sgg[+]

(Zeng and Verheyen, 2004)

Scer\GAL4^sd-SG29.1, nmo^c5-1.UAS has wing margin phenotype, suppressible by sgg^M11/sgg[+]

(Zeng and Verheyen, 2004)

Scer\GAL4^69B, nmo^c5-1.UAS has wing vein | ectopic phenotype, suppressible by Jra¹/Jra[+]

(Mirkovic et al., 2002)

Scer\GAL4^69B, nmo^c5-1.UAS has wing vein | ectopic phenotype, suppressible by msn[+]/msn^j1E2

(Mirkovic et al., 2002)

Scer\GAL4^69B, nmo^c5-1.UAS has posterior crossvein phenotype, suppressible by Tak1^K46R.UAS/Scer\GAL4^69B

(Mirkovic et al., 2002)

Scer\GAL4^69B, nmo^c5-1.UAS has posterior crossvein phenotype, suppressible by Df(2L)flp147E/+

(Mirkovic et al., 2002)

Scer\GAL4^69B, nmo^c5-1.UAS has posterior crossvein phenotype, suppressible by Jra¹/Jra[+]

(Mirkovic et al., 2002)

Scer\GAL4^69B, nmo^c5-1.UAS has posterior crossvein phenotype, suppressible by bsk¹/bsk[+]

(Mirkovic et al., 2002)

Scer\GAL4^69B, nmo^c5-1.UAS has posterior crossvein phenotype, suppressible by msn[+]/msn^j1E2

(Mirkovic et al., 2002)

Scer\GAL4^69B, nmo^c5-1.UAS has wing vein | ectopic phenotype, suppressible by bsk¹/bsk[+]

(Mirkovic et al., 2002)

Scer\GAL4^69B, nmo^c5-1.UAS has wing vein | ectopic phenotype, suppressible by Df(2L)flp147E/+

(Mirkovic et al., 2002)

Suppressor of

Statement

Reference

Scer\GAL4^71B/nmo^c5-1.UAS is a suppressor | partially of wing vein phenotype of Scer\GAL4^71B, arm^{ΔN.UAS.Tag:HA,Tag:Myr(Unk)}

(Zeng and Verheyen, 2004)

Scer\GAL4^71B/nmo^c5-1.UAS is a suppressor | partially of wing phenotype of Scer\GAL4^71B, arm^{ΔN.UAS.Tag:HA,Tag:Myr(Unk)}

(Zeng and Verheyen, 2004)

Scer\GAL4^hs.2sev/nmo^c5-1.UAS is a suppressor | partially of ommatidium phenotype of Scer\GAL4^hs.2sev, sca^UAS.cEa

(Chou and Chien, 2002)

Additional Comments

Genetic Interactions

Statement

Reference

Notching of the distal wing margin in nmo^{c5-1.Scer\UAS}; Scer\GAL4^sd-SG29.1 flies is completely suppressed by sgg^M11/+. Loss of notum macrochaetae in nmo^{c5-1.Scer\UAS}; Scer\GAL4^ap-md544 flies is suppressed by sgg^M11/+ and enhanced by Axn^A2.Scer\UAS, leading to the loss of all scutellar bristles. Severe blistering of the wing in nmo^{c5-1.Scer\UAS}; Scer\GAL4^ap-md544 flies is suppressed by sgg^M11/+, resulting in almost wild-type wings. The wing phenotype of arm^{Δ.Scer\UAS.T:Ivir\HA1}; Scer\GAL4^71B flies is almost completely suppressed by nmo^{c5-1.Scer\UAS}.

(Zeng and Verheyen, 2004)

The under-rotation defect exhibited by the ommatidia in the eye discs of sca^Scer\UAS.cEa; Scer\GAL4^hs.2sev larvae, is partially suppressed by nmo^{c5-1.Scer\UAS}.

(Chou and Chien, 2002)

The increase in cell death seen in Scer\GAL4^69B with nmo^{c5-1.Scer\UAS} embryos is supressed by co-expression of BacA\p35 from BacA\p35^Scer\UAS.cHa. The cuticles of these embryos are indistinguishable from those due to expression of BacA\p35 alone (BacA\p35^Scer\UAS.cHa; Scer\GAL4^69B) Heterozygosity for bsk¹ can suppress the wing-vein defects, as well as wing shape and size defects seen in Scer\GAL4^69B, nmo^{c5-1.Scer\UAS} adults. Heterozygosity for Jra¹ can suppress the wing-vein defects, as well as wing shape and size defects seen in Scer\GAL4^69B, nmo^{c5-1.Scer\UAS} adults. Heterozygosity for msn^j1E2 can suppress the wing-vein defects, as well as wing shape and size defects seen in Scer\GAL4^69B, nmo^{c5-1.Scer\UAS} adults. Heterozygosity for Df(2L)flp147E can suppress the wing-vein defects, and partially suppress the wing shape and size defects seen in Scer\GAL4^69B, nmo^{c5-1.Scer\UAS} adults. Tak1^{K46R.Scer\UAS} rescues the posterior cross vein, wing shape and size defects seen in Scer\GAL4^69B, nmo^{c5-1.Scer\UAS} adults, but enhances the L2 extra vein phenotype.

(Mirkovic et al., 2002)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

nmo^c5-1.UAS/Scer\GAL4^bs-1348 rescues nmo^adk2/nmo^adk1

(Verheyen et al., 2001)

Comments

Expression of nmo^{c5-1.Scer\UAS} under the control of Scer\GAL4^bs-1348 rescues the extra wing vein phenotype seen in nmo^adk1/nmo^adk2 flies.

(Verheyen et al., 2001)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

nmo^{c5-1.Scer\UAS}

nmo^c5-1.UAS

Name Synonyms

Secondary FlyBase IDs

References (7)

Report Sections

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